1. In Vitro Method of Studying the Angiotensin Activity of Chemical Compounds
- Author
-
Alexander A. Spasov, T. M. Bukatina, D. S. Yakovlev, and A. A. Brigadirova
- Subjects
Male ,Angiotensin receptor ,Drug Evaluation, Preclinical ,In Vitro Techniques ,Pharmacology ,Losartan ,Muscle, Smooth, Vascular ,General Biochemistry, Genetics and Molecular Biology ,Inhibitory Concentration 50 ,chemistry.chemical_compound ,Renin–angiotensin system ,medicine ,Animals ,Rats, Wistar ,IC50 ,Angiotensin II receptor type 1 ,biology ,Portal Vein ,Antagonist ,Angiotensin-converting enzyme ,General Medicine ,chemistry ,Vasoconstriction ,biology.protein ,Female ,Saralasin ,Angiotensin II Type 1 Receptor Blockers ,Muscle Contraction ,medicine.drug - Abstract
We developed and tested an experimental model to study in vitro the type 1 angiotensin antagonistic activity of compounds on the isolated portal vein of rats. The reliability of this method was confirmed in tests with saralasin (nonselective antagonist of angiotensin receptors) and losartan (selective antagonist of type 1 angiotensin receptors) in concentrations of 10(-9)-10(-5) mol/liter. The half-maximal inhibitory concentrations (IC50) of these substances were calculated.
- Published
- 2014