1. Transcriptional Activity of Human Endogenous Retroviruses in Human Peripheral Blood Mononuclear Cells
- Author
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Claudia Matteucci, Ayele Argaw-Denboba, Ilaria Bucci, Paola Sinibaldi-Vallebona, Chiara Cipriani, Francesca Pica, R Sorrentino, Emanuela Balestrieri, and Rossella Zenobi
- Subjects
Adult ,Male ,Transcriptional Activation ,Aging ,Article Subject ,Adolescent ,Transcription, Genetic ,viruses ,Population ,lcsh:Medicine ,Endogenous retrovirus ,Disease ,Biology ,Peripheral blood mononuclear cell ,General Biochemistry, Genetics and Molecular Biology ,Young Adult ,Basal (phylogenetics) ,Transcription (biology) ,Humans ,Child ,education ,Cells, Cultured ,Aged ,Aged, 80 and over ,Genetics ,education.field_of_study ,General Immunology and Microbiology ,Endogenous Retroviruses ,lcsh:R ,Infant ,General Medicine ,Middle Aged ,Settore MED/07 - Microbiologia e Microbiologia Clinica ,3. Good health ,Real-time polymerase chain reaction ,Child, Preschool ,embryonic structures ,Immunology ,Leukocytes, Mononuclear ,Female ,Human Pathology ,Research Article - Abstract
Human endogenous retroviruses (HERVs) have been implicated in human physiology and in human pathology. A better knowledge of the retroviral transcriptional activity in the general population and during the life span would greatly help the debate on its pathologic potential. The transcriptional activity of four HERV families (H, K, W, and E) was assessed, by qualitative and quantitative PCR, in PBMCs from 261 individuals aged from 1 to 80 years. Our results show that HERV-H, HERV-K, and HERV-W, but not HERV-E, are transcriptionally active in the test population already in the early childhood. In addition, the transcriptional levels of HERV-H, HERV-K, and HERV-W change significantly during the life span, albeit with distinct patterns. Our results, reinforce the hypothesis of a physiological correlation between HERVs activity and the different stages of life in humans. Studies aiming at identifying the factors, which are responsible for these changes during the individual’s life, are still needed. Although the observed phenomena are presumably subjected to great variability, the basal transcriptional activity of each individual, also depending on the different ages of life, must be carefully considered in all the studies involving HERVs as causative agents of disease.
- Published
- 2015