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1. Clinical and economic consequences of switching from omalizumab to mepolizumab in uncontrolled severe eosinophilic asthma

Author: M D'Amato, Giovanna Elisiana Carpagnano, Emanuela Resta, Nunzio Crimi, Massimiliano Povero, Girolamo Pelaia, Cecilia Calabrese, Onofrio Resta, Corrado Pelaia, Nicola Scichilone, Giulia Scioscia, Maria Pia Foschino Barbaro, Carpagnano G.E., Resta E., Povero M., Pelaia C., D'Amato M., Crimi N., Scichilone N., Scioscia G., Resta O., Calabrese C., Pelaia G., Barbaro M.P.F., Carpagnano, G. E., Resta, E., Povero, M., Pelaia, C., D'Amato, M., Crimi, N., Scichilone, N., Scioscia, G., Resta, O., Calabrese, C., Pelaia, G., and Barbaro, M. P. F.
Subjects: Spirometry, Male, medicine.medical_specialty, Exacerbation, Science, health status, Omalizumab, Settore MED/10 - Malattie Dell'Apparato Respiratorio, Antibodies, Monoclonal, Humanized, Article, Pulmonary function testing, 03 medical and health sciences, 0302 clinical medicine, Pharmacotherapy, Medical research, Internal medicine, medicine, Humans, Anti-Asthmatic Agent, 030212 general & internal medicine, Anti-Asthmatic Agents, Pulmonary Eosinophilia, Aged, Retrospective Studies, Respiratory tract diseases, Multidisciplinary, medicine.diagnostic_test, business.industry, Health care, Health care economics, Middle Aged, Asthma, Discontinuation, 030228 respiratory system, Exhaled nitric oxide, Medicine, Female, business, Mepolizumab, medicine.drug, Human
Abstract: Severe asthma is burdened by frequent exacerbations and use of oral corticosteroids (OCS), which worsen patients’ health and increase healthcare spending. The aim of this study was to assess the clinical and economic impact of switching from omalizumab (OMA) to mepolizumab (MEP) in patients eligible for both biologics, but not optimally controlled by omalizumab. We retrospectively enrolled uncontrolled severe asthmatic patients who switched from OMA to MEP during the last two years. Information included blood eosinophil count, asthma control test (ACT), spirometry, serum IgE, fractional exhaled nitric oxide (FeNO), OCS intake, drugs, exacerbations/hospitalizations, visits and diagnostic exams. Within the perspective of Italian National Health System, a pre- and post-MEP 12-month standardized total cost per patient was calculated. 33 patients were enrolled: five males, mean age 57 years, disease onset 24 years. At OMA discontinuation, 88% were OCS-dependent with annual mean rate of 4.0 clinically significant exacerbations, 0.30 exacerbations needing emergency room visits or hospitalization; absenteeism due to disease was 10.4 days per patient. Switch to MEP improved all clinical outcomes, reducing total exacerbation rate (RR = 0.06, 95% CI 0.03–0.14), OCS-dependent patients (OR = 0.02, 95% CI 0.005–0.08), and number of lost working days (Δ = − 7.9, 95% CI − 11.2 to − 4.6). Pulmonary function improved, serum IgE, FeNO and eosinophils decreased. Mean annual costs were €12,239 for OMA and €12,639 for MEP (Δ = €400, 95% CI − 1588–2389); the increment due to drug therapy (+ €1,581) was almost offset by savings regarding all other cost items (− €1,181). Patients with severe eosinophilic asthma, not controlled by OMA, experienced comprehensive benefits by switching to MEP with only slight increases in economic costs.
Published: 2021

2. Hydrogen sulfide and miR21 are suitable biomarkers of hypoxic exposure

Author: Selina M. Garcia, Benjamin Matheson, Juan H. Morales-Loredo, Nikki L. Jernigan, Nancy L. Kanagy, Thomas C. Resta, Ross M. Clark, Reza Shekarriz, and Laura V. Gonzalez Bosc
Subjects: Male, Oxygen, MicroRNAs, Endothelin-1, Physiology, Physiology (medical), Animals, Hydrogen Sulfide, Hypoxia, Biomarkers, Rats
Abstract: Hypoxia is the reduction of alveolar partial pressure of oxygen ([Formula: see text]). Military members and people who practice recreational activities from moderate to high altitudes are at risk for hypoxic exposure. Hypoxemia’s signs and symptoms vary from asymptomatic to severe responses, such as excessive hypoxic ventilatory responses and residual neurobehavioral impairment. Therefore, it is essential to identify hypoxia-induced biomarkers to indicate people with exposure to hypoxia. Advances have been made in understanding physiological responses to hypoxia, including elevations in circulating levels of endothelin 1 (ET-1) and microRNA 21 (miR-21) and reduction in circulating levels of hydrogen sulfide (H2S). Although the levels of these factors change upon exposure to hypoxia, it is unclear if these changes are sustained on return to normoxia. We hypothesize that hypoxia-induced ET-1 and miR-21 remain elevated, whereas hypoxia-reduction in H2S sustains after returning to normoxic conditions. To test this hypothesis, we exposed male rats to 6 h of 12% O2 and measured circulating levels of ET-1 and miR-21, pre, during, and posthypoxia. We found that ET-1 plasma levels increased in response to hypoxia but returned to normal levels within 30 min after the restoration of normoxia. miR-21 plasma levels and transdermal H2S emissions decreased in response to hypoxia, remaining decreased on return to normoxia, thus following the biomarker criteria. Therefore, this study supports a unique role for plasma miR21 and transdermal H2S as hypoxia biomarkers that could be used to identify individuals after exposure to hypoxia.
Published: 2023

3. Practice resource‐focused revision: Standardized pedigree nomenclature update centered on sex and gender inclusivity: A practice resource of the National Society of Genetic Counselors

Author: Robin L. Bennett, Kathryn Steinhaus French, Robert G. Resta, and Jehannine Austin
Subjects: Male, Counselors, Infant, Newborn, Humans, Gender Identity, Female, Societies, Transgender Persons, Genetics (clinical), Pedigree
Abstract: This focused revision builds on the expert opinions from the original publications of 'Recommendations for human standardized pedigree nomenclature' published in 1995 and updated in 2008. Our review of medical publications since 2008 did not identify any fundamental systematic alternative pedigree nomenclature. These findings attest to the relevance of most of the nomenclature with the critical exception of the nomenclature used to denote sex assigned at birth and gender. While we are not recommending the creation of any new pedigree symbols, a major focus of this publication is clarification of the use of symbols and language in the description of the distinction between sex and gender, with a view to ensuring safe and inclusive practice for people who are gender-diverse or transgender. In addition, we recommend modifications to the way that carrier status is depicted. Our goal is to respect individual differences and identities while maintaining biologically, clinically, and genetically meaningful information.
Published: 2022
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4. Impact of smoking, COPD and comorbidities on the mortality of COVID-19 patients

Author: Eugenio Sabato, Giovanna Elisiana Carpagnano, Onofrio Resta, Emanuela Resta, Carla Santomasi, Paolo Fuso, Franco Mastroianni, Donato Lacedonia, Maria Pia Foschino Barbaro, Andrea Portacci, Giulia Scioscia, Giovanni Larizza, and Emanuela Profilo
Subjects: Male, medicine.medical_specialty, Science, Comorbidity, Pathogenesis, Microbiology, Article, Cohort Studies, Pulmonary Disease, Chronic Obstructive, Intensive care, Internal medicine, medicine, Prevalence, Humans, Mortality, Signs and symptoms, Aged, COPD, Multidisciplinary, business.industry, Smoking, COVID-19, Middle Aged, Former Smoker, medicine.disease, Prognosis, respiratory tract diseases, Hospitalization, Pneumonia, Respiratory failure, Italy, Risk factors, Cohort, behavior and behavior mechanisms, Medicine, business, Cohort study
Abstract: The prognosis of the coronavirus disease 2019 (COVID-19) patients is variable and depends on several factors. Current data about the impact of chronic obstructive pulmonary disease (COPD) and smoking on the clinical course of COVID-19 are still controversial. This study evaluated the prevalence and the prognosis of COPD patients and smokers in a cohort of 521 patients admitted to four intermediate Respiratory Intensive Care Units (Puglia, Italy) with respiratory failure due to COVID-19 pneumonia. The prevalence of COPD and current smokers was 14% and 13%, respectively. COPD patients had a higher 30-day all-cause mortality than non-COPD patients. Former smokers compared to never smokers and current smokers had higher 30-day all-cause mortality. COPD patients and former smokers had more comorbidities. This study described the prevalence and the outcomes of COPD patients and smokers in a homogenous cohort of COVID-19 patients. The study showed that the prevalence of COPD and current smokers was not high, suggesting that they were not at increased risk of getting the infection. However, when SARS-CoV-2 infection occurred, COPD patients and former smokers were those with the highest all-cause mortality, which seemed to be mainly related to the presence of comorbidities and not to COPD and smoking itself.
Published: 2021

5. Suicidal behaviour in older age: A systematic review of risk factors associated to suicide attempts and completed suicides

Author: Cesare Cerri, Giancarlo Logroscino, Giuseppe Berardino, Antonello Bellomo, Massimiliano Beghi, Mario Altamura, Francesca Febbo, Emanuela Resta, Cesare Maria Cornaggia, Francesco Panza, Daniele Piscitelli, Anita Mollica, Elisa Butera, Antonio Daniele, Madia Lozupone, Beghi, M, Butera, E, Cerri, C, Cornaggia, C, Febbo, F, Mollica, A, Berardino, G, Piscitelli, D, Resta, E, Logroscino, G, Daniele, A, Altamura, M, Bellomo, A, Panza, F, and Lozupone, M
Subjects: Male, medicine.medical_specialty, Psychotropic drugs, Bipolar Disorder, Completed, Cognitive Neuroscience, Population, Psychological intervention, Suicide, Attempted, Late-life depression, Suicide prevention, Psychological factors, Suicidal Ideation, 03 medical and health sciences, Behavioral Neuroscience, 0302 clinical medicine, Risk Factors, Psychotropic drug, Stressful event, Suicide, Completed, Suicide attempt, medicine, Humans, 0501 psychology and cognitive sciences, Psychological factor, 050102 behavioral science & comparative psychology, Methods to self-harm, Psychiatry, education, Stressful events, Depression (differential diagnoses), Attempted, Aged, education.field_of_study, business.industry, 05 social sciences, Completed suicide, Late life depression, Suicide, Settore MED/26 - NEUROLOGIA, Neuropsychology and Physiological Psychology, Older age suicide, Anxiety, medicine.symptom, business, 030217 neurology & neurosurgery
Abstract: In older age, several observational studies investigated risk factors for suicide attempts/completed suicides; however, contrasting evidence came from population-based setting. In the present systematic review, we described through a narrative synthesis the significant associations existing among risk factors and suicide attempts/completed suicides in subjects aged > 65 years. From the 39 population-based studies selected in six different databases until February 15, 2021, we analyzed the most frequent 28 risk factors for suicidal behaviour. The risk factors more associated to suicide attempts than other variables frequently related to suicidal behavior in older age were: depressive disorders, methods employed to self-harm (particularly poisoning), and psychotropic drug utilization followed by psychological factors and disability. Moreover, male sex, violent methods to self-harm, any psychiatric disorder (depression, anxiety and bipolar disorders), a poor medical condition, stressors/bereavement, and living alone appeared to be more significant for predicting completed suicides in late life. In older age, efforts for suicide prevention should be based on strategies to assess and treat psychiatric disorders along with psychological interventions, particularly in males.
Published: 2021
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6. Influence of the Weekly and Match-play Load on Muscle Injury in Professional Football Players

Author: Víctor, Moreno-Perez, Miguel Angel, Campos-Vazquez, Javier, Toscano, Victor Javier, Sotos-Martinez, Roberto, López-Del Campo, Ricardo, Resta, and Juan, Del Coso
Subjects: Male, Muscles, Soccer, Football, Humans, Orthopedics and Sports Medicine, Physical Therapy, Sports Therapy and Rehabilitation, Athletic Performance, Running
Abstract: The aim of this investigation was to examine the impact of the weekly training load and the match running patterns prior to a muscle injury as potential risk factors of muscle injury in professional football players. Forty male professional football players participated in the investigation. Running distances at different intensities 5 min and 15 min prior to the injury were compared to the same time-points in official matches of the same player with no injury events. Furthermore, the cummulative session rating of perceived exertion (sRPE) and training load of the week prior to the injury were compared to a control week (mean value of training weeks without injury). Nineteen players suffered 31 non-contact muscle injuries during matches. The distance covered at 21–24 km/h (p24 km/h (p=0.004; ES=0.51) over the 5-min period prior to the injury was greater than in matches without injury. The cumulative sRPE (p=0.014; ES=1.33) and training volume (p=0.002; ES=2.45) in the week prior to the injury was higher than in a control week. The current data suggest that the combination of a training week with a high load and a short period of high intensity running during the match might increase the risk of muscle injury in professional footballers.
Published: 2022
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7. Justice beliefs and cultural values predict support for COVID-19 vaccination and quarantine behavioral mandates

Author: Todd, Lucas, Mark, Manning, Peter, Strelan, Catalina, Kopetz, Maximilian, Agostini, Bélanger, Jocelyn J., Ben, Gützkow, Jannis, Kreienkamp, Georgios, Abakoumkin, Jamilah Hanum Abdul Khaiyom, Vjollca, Ahmedi, Handan, Akkas, Almenara, Carlos A., Mohsin, Atta, Sabahat Cigdem Bagci, Sima, Basel, Edona Berisha Kida, Bernardo, Allan B. I., Buttrick, Nicholas R., Phatthanakit, Chobthamkit, Hoon-Seok, Choi, Mioara, Cristea, Sára, Csaba, Kaja, Damnjanovic, Ivan, Danyliuk, Arobindu, Dash, DI SANTO, Daniela, Douglas, Karen M., Violeta, Enea, Daiane Gracieli Faller, Gavan, Fitzsimons, Alexandra, Gheorghiu, Ángel, Gómez, Joanna, Grzymala-Moszczynska, Ali, Hamaidia, Qing, Han, Mai, Helmy, Joevarian, Hudiyana, Jeronimus, Bertus F., Ding-Yu, Jiang, Veljko, Jovanović, Željka, Kamenov, Anna, Kende, Shian-Ling, Keng, Tra Thi Thanh Kieu, Yasin, Koc, Kamila, Kovyazina, Inna, Kozytska, Joshua, Krause, Kruglanski, Arie W., Anton, Kurapov, Maja, Kutlaca, Nóra Anna Lantos, Edward, P. Lemay Jr., Cokorda Bagus Jaya Lesmana, Louis, Winnifred R., Adrian, Lueders, Najma Iqbal Malik, Anton, Martinez, Mccabe, Kira O., Jasmina, Mehulić, Mirra Noor Milla, Idris, Mohammed, Erica, Molinario, Manuel, Moyano, Hayat, Muhammad, Silvana, Mula, Hamdi, Muluk, Solomiia, Myroniuk, Reza, Najafi, Nisa, Claudia F., Boglárka, Nyúl, O’Keefe, Paul A., Jose Javier Olivas Osuna, Osin, Evgeny N., Joonha, Park, Gennaro, Pica, Antonio, Pierro, Jonas, Rees, Anne Margit Reitsema, Elena, Resta, Marika, Rullo, Ryan, Michelle K., Adil, Samekin, Pekka, Santtila, Edyta, Sasin, Schumpe, Birga M., Selim, Heyla A., Michael Vicente Stanton, Wolfgang, Stroebe, Sutton, Robbie M., Eleftheria, Tseliou, Akira, Utsugi, Jolien Anne van Breen, Van Lissa, Caspar J., Kees Van Veen, Vandellen, Michelle R., Alexandra, Vázquez, Robin, Wollast, Victoria Wai-lan Yeung, Somayeh, Zand, Iris Lav Žeželj, Bang, Zheng, Andreas, Zick, Claudia, Zúñiga, Pontus Leander, N., Social Psychology, Developmental Psychology, and Organizational Psychology
Subjects: Adult, Male, COVID-19 Vaccines, vaccination, justice, cultural values, COVID-19, SARS-CoV-2, Brief Report, Vaccination, Values, AcademicSubjects/SCI02170, Just world beliefs, Behavioral Neuroscience, Cultural dimensions, Social Justice, Quarantine, Humans, AcademicSubjects/MED00860, Applied Psychology, covid-19, quarantine, just world beliefs, values, cultural dimensions
Abstract: Understanding how individual beliefs and societal values influence support for measures to prevent SARS-CoV-2 transmission is vital to developing and implementing effective prevention policies. Using both Just World Theory and Cultural Dimensions Theory, the present study considered how individual-level justice beliefs and country-level social values predict support for vaccination and quarantine policy mandates to reduce SARS-CoV-2 transmission. Data from an international survey of adults from 46 countries (N = 6424) were used to evaluate how individual-level beliefs about justice for self and others, as well as national values—that is, power distance, individualism, masculinity, uncertainty avoidance, long-term orientation, and indulgence—influence support for vaccination and quarantine behavioral mandates. Multilevel modeling revealed that support for vaccination and quarantine mandates were positively associated with individual-level beliefs about justice for self, and negatively associated with country-level uncertainty avoidance. Significant cross-level interactions revealed that beliefs about justice for self were associated more strongly with support for mandatory vaccination in countries high in individualism, whereas beliefs about justice for others were more strongly associated with support for vaccination and quarantine mandates in countries high in long-term orientation. Beliefs about justice and cultural values can independently and also interactively influence support for evidence-based practices to reduce SARS-CoV-2 transmission, such as vaccination and quarantine. Understanding these multilevel influences may inform efforts to develop and implement effective prevention policies in varied national contexts.
Published: 2022
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8. More skilled clinical management of COVID-19 patients modified mortality in an intermediate respiratory intensive care unit in Italy

Author: Onofrio Resta, Salvatore Grasso, Vito Procacci, Giovanni Migliore, Francesco Panza, Emanuela Resta, and Giovanna Elisiana Carpagnano
Subjects: Male, medicine.medical_specialty, Time Factors, Coronavirus disease 2019 (COVID-19), Survival, 030204 cardiovascular system & hematology, Severity of Illness Index, Assisted ventilation, law.invention, 03 medical and health sciences, Patient Admission, 0302 clinical medicine, law, Epidemiology, Pandemic, Severity of illness, medicine, Humans, Hospital Mortality, 030212 general & internal medicine, Respiratory system, Aged, Retrospective Studies, Aged, 80 and over, lcsh:RC705-779, business.industry, Research, COVID-19, Retrospective cohort study, lcsh:Diseases of the respiratory system, Length of Stay, Middle Aged, Intensive care unit, Intensive Care Units, Critical care, Treatment Outcome, Italy, Emergency medicine, Female, Observational study, Clinical Competence, business, Intermediate RICU
Abstract: BackgroundSome studies investigated epidemiological and clinical features of laboratory-confirmed patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) the virus causing coronavirus disease 2019 (COVID-19), but limited attention has been paid to the follow-up of hospitalized patients on the basis of clinical setting and the expertise of clinical management.MethodsIn the present single-centered, retrospective, observational study, we reported findings from 87 consecutive laboratory-confirmed COVID-19 patients with moderate-to-severe acute respiratory syndrome hospitalized in an intermediate Respiratory Intensive Care Unit (RICU), subdividing the patients in two groups according to the admission date (before and after March 29, 2020).ResultsWith improved skills in the clinical management of COVID-19, we observed a significant lower mortality in the T2 group compared with the T1 group and a significantly difference in terms of mortality among the patients transferred in Intensive Care Unit (ICU) from our intermediate RICU (100% in T1 group vs. 33.3% in T2 group). The average length of stay in intermediate RICU of ICU-transferred patients who survived in T1 and T2 was significantly longer than those who died (who died 3.3 ± 2.8 daysvs. who survived 6.4 ± 3.3 days). TConclusionsThe present findings suggested that an intermediate level of hospital care may have the potential to modify survival in COVID-19 patients, particularly in the present phase of a more skilled clinical management of the pandemic.
Published: 2021

9. Bilevel and continuous positive airway pressure and factors linked to all-cause mortality in COVID-19 patients in an intermediate respiratory intensive care unit in Italy

Author: Giovanni Migliore, Maria Luisa De Candia, Onofrio Resta, Valentina Di Lecce, Vincenzo Solfrizzi, Emanuela Resta, Enrico Buonamico, Francesco Panza, and Giovanna Elisiana Carpagnano
Subjects: Adult, Male, Pulmonary and Respiratory Medicine, 2019-20 coronavirus outbreak, medicine.medical_specialty, Critical Care, Coronavirus disease 2019 (COVID-19), medicine.medical_treatment, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Comorbidity, assisted ventilation, 03 medical and health sciences, intermediate RICU, 0302 clinical medicine, respiratory infection, Risk Factors, Cause of Death, medicine, Humans, Immunology and Allergy, Hospital Mortality, 030212 general & internal medicine, Continuous positive airway pressure, Original Research, Aged, Retrospective Studies, Aged, 80 and over, Respiratory Distress Syndrome, Respiratory intensive care unit, Continuous Positive Airway Pressure, SARS-CoV-2, business.industry, Public Health, Environmental and Occupational Health, COVID-19, Respiratory infection, Middle Aged, humanities, Hospitalization, Oxygen, Intensive Care Units, Italy, 030228 respiratory system, Emergency medicine, Female, Observational study, viral infection, business, All cause mortality, Research Article
Abstract: Objectives: In the present single-centered, retrospective, observational study, we reported findings from 78 consecutive laboratory-confirmed COVID-19 patients with moderate-to-severe acute respiratory distress syndrome (ARDS) hospitalized in an intermediate Respiratory Intensive Care Unit, subdividing the patients into two groups according to their clinical outcome, dead patients and discharged patients. Methods: We further subdivided patients depending on the noninvasive respiratory support used during hospitalization. Results: In those patients who died, we found significant older age and higher multimorbidity and higher values of serum lactate dehydrogenase, C-reactive protein, and D-dimer. Among patients who were submitted to bilevel positive airway pressure (BPAP), those who died had a significant shorter number of days in overall length of stay and lower values of arterial oxygen partial pressure to fractional inspired oxygen ratio (PaO2/FiO2 ratio) compared to those who survived. No difference in all-cause mortality was observed between the two different noninvasive respiratory support groups [48% for continuous positive airway pressure (CPAP) and 52% for BPAP]. Conclusion: In COVID-19 patients with moderate-to-severe ARDS using BPAP in an intermediate level of hospital care had more factors associated to all-cause mortality (shorter length of stay and lower baseline PaO2/FiO2 ratio) compared to those who underwent CPAP.
Published: 2020
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10. Apolipoprotein E genotype, inflammatory biomarkers, and non-psychiatric multimorbidity contribute to the suicidal ideation phenotype in older age. The Salus in Apulia Study

Author: Madia Lozupone, Rossella Donghia, Rodolfo Sardone, Anita Mollica, Giuseppe Berardino, Luisa Lampignano, Chiara Griseta, Roberta Zupo, Fabio Castellana, Ilaria Bortone, Vittorio Dibello, Emanuela Resta, Roberta Stallone, Davide Seripa, Antonio Daniele, Vincenzo Solfrizzi, Mario Altamura, Antonello Bellomo, Francesco Panza, and Oral Kinesiology
Subjects: Male, IL-6, Genotype, Apolipoprotein E4, Multimorbidity, Late-life depression, Asthma, Psychiatry and Mental health, Clinical Psychology, Settore MED/26 - NEUROLOGIA, Phenotype, C-Reactive Protein, SDG 3 - Good Health and Well-being, Risk Factors, Suicidal ideation, Animals, Female, Obesity, Biomarkers, APOE
Abstract: Background: Possible relationships between suicidal ideation and biopsychosocial predictors in older age are unclear. In the population-based Salus in Apulia Study, we investigated the relationships among biomarkers, socio-demographic, psychopathological, inflammatory and metabolic characteristics and suicidal ideation in 1252 older subjects. Methods: Suicidal ideation was evaluated with the brief version of the Columbia-Suicide Severity Rating Scale. Apolipoprotein E (APOE) genotype and inflammatory profile [interleukin (IL)-6, tumor necrosis factor (TNF)-α, C-reactive protein (CRP)] were evaluated. A machine learning algorithm, the Random Forest (RF), selected potential biopsychosocial factors associated to suicidal ideation. Results: Suicidal ideators accounted for 2.32 % of subjects, were female, smokers, and obese with multimorbidity. After adjusting for age, gender, education and social dysfunction, logistic regression analyses revealed that suicidal ideation was associated to late-life depression (LLD) (odds ratio:21.71,95 % confidence interval:9.22–51.14). In the full RF model, asthma was the most important contributor to suicidal ideation. In the final RF model, education, age, and mild cognitive impairment followed by gender and global cognition were considered the most important contributors. Among biomarkers, in the final RF model, IL-6 followed by TNF-α, APOE ε4 allele presence, CRP and high-density lipoprotein cholesterol contributed most to suicidal ideation. Limitations: A relatively small number of older subjects with suicidal ideation (2.3 %); we did not distinguish between active and passive suicidal ideation. Conclusions: Although LLD is a strong determinant of suicidal ideation, other non-psychiatric factors, i.e., serum inflammation biomarkers, APOE ε4 allele, and multimorbidity, should be taken into account when evaluating a suicidal ideation phenotype in older age.
Published: 2022
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11. Kaposiform hemangioendothelioma further broadens the phenotype of <scp>PIK3CA</scp> ‐related overgrowth spectrum

Author: Paola Coppo, Roberta La Selva, Giovanni Battista Ferrero, Paola Francia di Celle, Alessandro Mussa, Rosaria Manicone, Federica Santoro, Silvia Kalantari, Simona Cardaropoli, Nicoletta Resta, Franca Fagioli, Carlotta Ranieri, and Diana Carli
Subjects: Male, Pathology, medicine.medical_specialty, Genotype, Class I Phosphatidylinositol 3-Kinases, Biopsy, Kasabach-Merritt Syndrome, Vascular anomaly, Genetics, Humans, PROS, Medicine, Genetic Predisposition to Disease, Sarcoma, Kaposi, Alleles, Genetic Association Studies, Growth Disorders, Genetics (clinical), PI3K/AKT/mTOR pathway, business.industry, Kaposiform hemangioendothelioma, mixed vascular-lymphatic malformations, PIK3CA, PIK3CA-related overgrowth spectrum, Infant, medicine.disease, Immunohistochemistry, Phenotype, Lymphangiogenesis, Radiography, Lymphatic system, Amino Acid Substitution, Kaposiform Hemangioendothelioma, Hemangioendothelioma, Mutation, Vascular tumor, business
Abstract: Kaposiform hemangioendothelioma (KHE) is a rare locally aggressive mixed vascular tumor, with typical onset in early childhood and characterized by progressive angio- and lymphangiogenesis. Its etiopathogenesis and molecular bases are still unclear. Here, we report the first case of congenital KHE harboring a PIK3CA mosaic pathogenic variant (c.323G > A, p.Arg108His) in a boy with very subtle PIK3CA-related overgrowth spectrum (PROS) features. This finding provides insights into the pathophysiology of KHE, offering targeted therapeutic options by inhibition of the PI3K/Akt/mTOR pathway. We propose the inclusion of this mixed lymphatic and vascular anomaly within the PROS.
Published: 2021
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12. Effectiveness and Safety of A Nutraceutical Formulation for the Treatment of Functional Dyspepsia in Primary Care

Author: Giuseppe Sica, Maria Santa Alfieri, Antonio Pio Palieri, Pasquale Zecchillo, Luigi Mazzilli, Antonio Tursi, Francesco Detommasi, Fabrizio Bossa, Marcello Picchio, Giovanni Brandimarte, Leonardo Allegretta, Vincenzo De Cristofaro, Giovanni Sbarra, Michele Manfredi, Giovanni Messa, Antonio Penna, Pietro Derenzio, Basilio Paolo Colucci, Rocco Demauro, Saverio Nappi, Michele Fazio, Francesco Maurichi, Federica Greco, Francesco Monterisi, Alessio Gallo, Mario Tarallo, Pasquale Bellomo, Costantino Vigilante, Vito Lisco, Walter Elisei, Vincenzo Ferrari, Francesco Lore, Pasquale Saponaro, Antonio Elefante, Natale Antonino, Giorgio Marsano, Giancarlo Torelli, Anna Rosa Resta, Carlo Autorino, Ludovico Abenavoli, Giuseppe Pranzo, Simone Di Bella, Giulio Minoretti, Pierluigi Benedicenti, Stefano Gallina, Carlo Casamassima, Pasquale Mascoli, Salvatore Scutifero, Giovanni Luca Rizzo, Donatella Greco, Pasquale Misciagna, and Vincenzo Selvaggio
Subjects: Male, medicine.medical_specialty, Population, Primary care, Epigastric pain, Drug treatment, Nutraceutical, Internal medicine, medicine, Humans, Dyspepsia, education, Retrospective Studies, Pharmacology, Thymus extract, education.field_of_study, Primary Health Care, business.industry, General Medicine, Middle Aged, medicine.disease, Obesity, Abdominal Pain, Dietary Supplements, Large study, Female, business
Abstract: Background: Although FD may affect up to 10% of the general population, the therapy for FD is not standard. Recently, ginger-based food supplements have been proposed in order to restore FD symptoms. Our aim was to assess the efficacy of a new nutraceutical formulation containing extract of gingerol and thymus as a possible natural treatment in managing the symptoms of functional dyspepsia (FD). Methods: We retrospectively analyzed the efficacy and safety profiles of a nutraceutical formulation containing Zingiber officinalis root extract and a standardized Thymus extract. It was administered as 1 ml/day twice a day for 90 days. Patients were assessed at baseline and after 1, 2 and 3 months of treatment, following a month of pharmacological washout by completing a questionnaire reporting the trend of the following symptoms: epigastric pain, epigastric heaviness, early satiety, belching, and regurgitation. Every symptom was assessed by a Visual Analogic Scale (VAS), ranging from 0= absence to 10= maximal severity. Results: We enrolled 272 patients (99 males and 173 females; median IQR age 49.5, 36-64 yrs). Obesity (BMI>30) was present in 28 (12.5%) patients; smokers were 83 (30.5%); and comorbidities were present in 107 (39.3%) patients. Improvement of symptom scores during treatment and one month after its suspension was extremely significant (p Conclusion: This large study found that nutraceutical formulation could be one of the tools for an empirical approach to treat patients with FD, especially when a non-conventional drug treatment is preferable for the patient and considered suitable by the physician.
Published: 2021
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13. Postprandial glucose and HbA1c are associated with severity of obstructive sleep apnoea in non-diabetic obese subjects

Author: Angelo Cignarelli, Luigi Laviola, Vito Antonio Falcone, Vitaliano Nicola Quaranta, S. Kounaki, Sebastio Perrini, Maria Pia Foschino Barbaro, Francesco Giorgino, Onofrio Resta, Alessandro Ciavarella, A Di Trani, and Annalisa Natalicchio
Subjects: Adult, Blood Glucose, Male, medicine.medical_specialty, Waist, Endocrinology, Diabetes and Metabolism, Polysomnography, 030209 endocrinology & metabolism, Type 2 diabetes, Gastroenterology, Severity of Illness Index, Body Mass Index, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Risk Factors, Internal medicine, medicine, Prevalence, Humans, Obesity, Glycated Hemoglobin, Sleep Apnea, Obstructive, medicine.diagnostic_test, business.industry, Postprandial, Sleep apnea, Fasting, Glucose Tolerance Test, medicine.disease, Postprandial Period, respiratory tract diseases, Blood pressure, Hyperglycemia, Obese subjects, Original Article, Female, Glycemia, business, 030217 neurology & neurosurgery
Abstract: Introduction Obstructive sleep apnoea (OSA) is an underdiagnosed condition frequently associated with glycaemic control impairment in patients with type 2 diabetes. Aim To assess the relationship between glycometabolic parameters and OSA in obese non-diabetic subjects. Methods Ninety consecutive subjects (mean age 44.9 ± 12 years, mean BMI 42.1 ± 9 kg/m2) underwent polysomnography and a 2-h oral glucose tolerance test (OGTT). Results OSA was identified in 75% of subjects, with a higher prevalence of males compared to the group of subjects without OSA (62% vs 32%, p = 0.02). Patients with OSA had comparable BMI (42.8 kg/m2 vs 39.4 kg/m2), a higher average HbA1c (5.8% vs 5.4%, p p = 0.009) and diastolic blood pressure (81.1 mmHg vs 76.2 mmHg, p = 0.046) than obese subjects without OSA. HbA1c and 2 h-PG were found to be correlated with the apnoea-hypopnoea index (AHI; r = 0.35 and r = 0.42, respectively) and with percent of sleep time with oxyhaemoglobin saturation r = 0.44 and r = 0.39, respectively). Further, in a linear regression model, ST90 and AHI were found to be the main determinants of 2 h-PG (β = 0.81, p β = 0.75, p = 0.02, respectively) after controlling for age, sex, waist circumference, physical activity, and C-reactive protein. Similarly, ST90 and AHI persisted as independent determinants of HbA1c (β = 0.01, p = 0.01 and β = 0.01, p = 0.01, respectively). Conclusion Beyond the traditional clinical parameters, the presence of a normal-high value of 2 h-PG and HbA1c should raise suspicion of the presence of OSA in obese subjects.
Published: 2021

14. Late gadolinium enhancement role in arrhythmic risk stratification of patients with LMNA cardiomyopathy: results from a long-term follow-up multicentre study

Author: Arnaldo Scardapane, Paolo Della Bella, Martino Pepe, Cinzia Forleo, Giovanni Peretto, Chiara Di Resta, Andrea Igoren Guaricci, Anna Santoni, Michele Emdin, Vincenzo Ezio Santobuono, Andrea Barison, Antonio Esposito, Simone Sala, Sara Benedetti, Maurizio Ferrari, Stefano Favale, Anna Palmisano, Giovanni Donato Aquaro, Nicoletta Resta, Peretto, Giovanni, Barison, Andrea, Forleo, Cinzia, Di Resta, Chiara, Esposito, Antonio, Aquaro, Giovanni Donato, Scardapane, Arnaldo, Palmisano, Anna, Emdin, Michele, Resta, Nicoletta, Santoni, Anna, Guaricci, Andrea Igoren, Santobuono, Vincenzo Ezio, Pepe, Martino, Favale, Stefano, Ferrari, Maurizio, Benedetti, Sara, Della Bella, Paolo, and Sala, Simone
Subjects: Male, Cardiac magnetic resonance, Cardiomyopathy, Contrast Media, Gadolinium, 030204 cardiovascular system & hematology, Late gadolinium enhancement, Ventricular Function, Left, 030218 nuclear medicine & medical imaging, Sudden cardiac death, LMNA, 0302 clinical medicine, Risk Factors, Clinical endpoint, LMNA mutation, Ejection fraction, Middle Aged, Lamin Type A, Defibrillators, Implantable, embryonic structures, cardiovascular system, Cardiology, Female, Cardiology and Cardiovascular Medicine, Cardiomyopathies, Adult, medicine.medical_specialty, Adolescent, Risk Assessment, 03 medical and health sciences, Young Adult, Ventricular arrhythmias, Linear gingival erythema, Predictive Value of Tests, Physiology (medical), Internal medicine, medicine, Humans, cardiovascular diseases, Risk stratification, business.industry, Stroke Volume, medicine.disease, Ventricular fibrillation, Implant, business, Follow-Up Studies
Abstract: Aims We aimed at addressing the role of late gadolinium enhancement (LGE) in arrhythmic risk stratification of LMNA-associated cardiomyopathy (CMP). Methods and results We present data from a multicentre national cohort of patients with LMNA mutations. Of 164 screened cases, we finally enrolled patients with baseline cardiac magnetic resonance (CMR) including LGE sequences [n = 41, age 35 ± 17 years, 51% males, mean left ventricular ejection fraction (LVEF) by echocardiogram 56%]. The primary endpoint of the study was follow-up (FU) occurrence of malignant ventricular arrhythmias [MVA, including sustained ventricular tachycardia (VT), ventricular fibrillation, and appropriate implantable cardioverter-defibrillator (ICD) therapy]. At baseline CMR, 25 subjects (61%) had LGE, with non-ischaemic pattern in all of the cases. Overall, 23 patients (56%) underwent ICD implant. By 10 ± 3 years FU, eight patients (20%) experienced MVA, consisting of appropriate ICD shocks in all of the cases. In particular, the occurrence of MVA in LGE+ vs. LGE− groups was 8/25 vs. 0/16 (P = 0.014). Of note, no significant differences between LGE+ and LGE− patients were found in currently recognized risk factors for sudden cardiac death (male gender, non-missense mutations, baseline LVEF 0.05. Conclusions In LMNA-CMP patients, LGE at baseline CMR is significantly associated with MVA. In particular, as suggested by this preliminary experience, the absence of LGE allowed to rule-out MVA at 10 years mean FU.
Published: 2020

15. [ANMCO Position paper: Cardiovascular disease in women - prevention, diagnosis, treatment and organization of care]

Author: Francese, G. M., Aspromonte, N., Valente, S., Geraci, G., Pavan, D., Bisceglia, I., Caforio, A. L. P., Colavita, A. R., Cutolo, A., De Angelis, M. C., Di Fusco, S. A., Enea, I., Fiscella, D., Frongillo, D., V. G., Ad, Giubilato, S., Giuffrida, C., Ingianni, N., Luca, F., Marcantoni, L., Martinis, F., Marzullo, R., Masciocco, G., Parrini, I., Rakar, S., Resta, M., Riva, L., Rossini, R., Russo, D., Russo, G., Russo, M. G., Scardovi, A. B., De Luca, L., Gabrielli, D., Gulizia, M. M., Oliva, F., and Colivicchi, F.
Subjects: Male, Sex Factors, Cardiovascular Diseases, Risk Factors, Biological differences, Gender differences, Humans, Estrogens, Female, Cardiovascular disease, Prognosis
Abstract: Cardiovascular diseases are still the main cause of death among women despite the improvements in treatment and prognosis achieved in the last 30 years of research. The determinant factors and causes have not been completely identified but the role of "gender" is now recognized. It is well known that women tend to develop cardiovascular disease at an older age than men, and have a high probability of manifesting atypical symptoms not often recognized. Other factors may also co-exist in women, which may favor the onset of specific cardiac diseases such as those with a sex-specific etiology (differential effects of estrogens, pregnancy pathologies, etc.) and those with a different gender expression of specific and prevalent risk factors, inflammatory and autoimmune diseases and cancer. Whether the gender differences observed in cardiovascular outcomes are influenced by real biological differences remains a matter of debate.This ANMCO position paper aims at providing the state of the research on this topic, with particular attention to the diagnostic aspects and to care organization.
Published: 2022

16. Body Composition Asymmetries in University Ice Hockey Players and Their Implications for Lower Back Pain and Leg Injury

Author: Tiziana Resta, Stephane Frenette, Amanda Rizk, and Maryse Fortin
Subjects: Male, Cross-Sectional Studies, Hockey, Universities, Body Composition, Humans, Physical Therapy, Sports Therapy and Rehabilitation, Orthopedics and Sports Medicine, Female, General Medicine, Low Back Pain, Leg Injuries
Abstract: Resta, T, Frenette, S, Rizk, A, and Fortin, M. Body composition asymmetries in university ice hockey players and their implications for lower back pain and leg injury. J Strength Cond Res 36(10): 2830-2836, 2022-Right to left asymmetries in body composition have been examined across many sports, suggesting possible implications for lower back pain (LBP) and decreased level of performance. However, we are not aware of any study that has examined the presence and implications of morphological asymmetries in ice hockey players. The purpose of this study was to (a) investigate body composition asymmetries in female and male university-level ice hockey players and (b) examine whether the degree of body composition asymmetry is associated with the history of LBP and lower-limb injury (LLI). A total of 32 players (female = 18, male = 14) were included in this cross-sectional study (e.g., university research center setting). Dual-energy X-ray absorptiometry (DEXA) was used to acquire body composition measurements. The parameters of interest included bone mass, lean body mass, and fat mass, for the right and left sides and body segments (e.g., arm, leg, trunk, and total), separately. The history of LBP and LLI was obtained using a self-reported demographic questionnaire. The statistical significance for the study was set at p0.05. Our findings revealed significant side-to-side asymmetry in arm and total bone mass in females, with higher values on the right side. Both males and females also had significantly greater trunk lean body mass on the left side. With the exception of greater arm bone mass asymmetry being associated with LBP in the past 3 months, there was no other significant association between the degree of asymmetry with LBP and LLI. This study provides novel data regarding the presence of asymmetry in body composition in university-level ice hockey players. Monitoring body composition in athletes provides information that can be used by athletic trainers and strength and conditioning coaches to develop injury prevention, performance optimization, and targeted rehabilitation programs.
Published: 2022

17. Novel SCN5A p.W697X Nonsense Mutation Segregation in a Family with Brugada Syndrome

Author: Giuseppe Ciconte, Emanuela T Locati, Luigi Giannelli, Nicoletta Resta, Luigi Anastasia, Valeria Borrelli, Michelle M. Monasky, Gabriele Vicedomini, Andrea Ghiroldi, Chiara Di Resta, Rosanna Bagnulo, Sara Benedetti, Maurizio Ferrari, Carlo Pappone, Emanuele Micaglio, Micaglio, E, Monasky, M M, Resta, N, Bagnulo, R, Ciconte, G, Gianelli, L, Locati, E T, Vicedomini, G, Borrelli, V, Ghiroldi, A, Anastasia, L, Benedetti, S, Di Resta, C, Ferrari, M, and Pappone, C
Subjects: 0301 basic medicine, Male, Genetic testing, family, Case Report, 030204 cardiovascular system & hematology, Sudden cardiac death, NAV1.5 Voltage-Gated Sodium Channel, lcsh:Chemistry, 0302 clinical medicine, Channelopathy, lcsh:QH301-705.5, Spectroscopy, SCN5A, Brugada syndrome, Genetics, medicine.diagnostic_test, Sodium channel, scn5a, General Medicine, Middle Aged, Computer Science Applications, Pedigree, Codon, Nonsense, Mutation (genetic algorithm), Female, Arrhythmia, point-nonsense mutation, Human, sodium channel, Adult, Nonsense mutation, Biology, arrhythmia, Catalysis, sudden cardiac death, genetic testing, Inorganic Chemistry, 03 medical and health sciences, channelopathy, medicine, Family, Functional studies, Physical and Theoretical Chemistry, Molecular Biology, Gene, Organic Chemistry, fungi, Point-nonsense mutation, medicine.disease, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Mutation, mutation, brugada syndrome
Abstract: Brugada syndrome (BrS) is marked by an elevated ST-segment elevation and increased risk of sudden cardiac death. Variants in the SCN5A gene are considered to be molecular confirmation of the syndrome in about one third of cases, while the genetics remain a mystery in about half of the cases, with the remaining cases being attributed to variants in any of a number of genes. Before research models can be developed, it is imperative to understand the genetics in patients. Even data from humans is complicated, since variants in the most common gene in BrS, SCN5A, are associated with a number of pathologies, or could even be considered benign, depending on the variant. Here, we provide crucial human data on a novel NM_198056.2:c.2091G>A (p.Trp697X) point-nonsense heterozygous variant in the SCN5A gene, as well as its segregation with BrS. The results herein suggest a pathogenic effect of this variant. These results could be used as a stepping stone for functional studies to better understand the molecular effects of this variant in BrS.
Published: 2019

18. The Surgical Management of Acute Gastric Volvulus: Clinical Outcomes and Quality of Life Assessment

Author: Savino Occhionorelli, Giorgio Cavallesco, Chiara Vigolo, S. Marino, Giuseppe Resta, Gabriele Anania, Serena Rubino, Nicola Tamburini, Marcello Sanzi, Giorgio Vasquez, and Ciro Andolfi
Subjects: Male, musculoskeletal diseases, medicine.medical_specialty, Stomach Volvulus, NO, Hiatal hernia, Postoperative Complications, Quality of life, Recurrence, Surveys and Questionnaires, parasitic diseases, Humans, Medicine, stomach diseases, Postoperative Period, Laparoscopy, Aged, Retrospective Studies, Aged, 80 and over, Gastric volvulus, stomach volvulus, laparoscopy, stomach diseases, hiatal hernia, quality of life, medicine.diagnostic_test, business.industry, Middle Aged, medicine.disease, digestive system diseases, Surgery, Hernia, Hiatal, Treatment Outcome, Patient Satisfaction, Acute Disease, Quality of Life, Female, Acute gastric volvulus, business, hiatal hernia
Abstract: Background: Surgery is the mainstay of treatment for gastric volvulus. Despite its rarity, early experience from recent publications suggests that laparoscopy is a safe and effective approach for t...
Published: 2021
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19. EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count

Author: Stefania Paolucci, Irene Cassaniti, Federica Novazzi, Loretta Fiorina, Antonio Piralla, Giuditta Comolli, Raffaele Bruno, Renato Maserati, Roberto Gulminetti, Stefano Novati, Francesco Mojoli, Fausto Baldanti, R Bruno, M Mondelli, E Brunetti, A Di Matteo, E Seminari, L Maiocchi, V Zuccaro, L Pagnucco, B Mariani, S Ludovisi, R Lissandrin, A Parisi, P Sacchi, SFA Patruno, G Michelone, R Gulminetti, D Zanaboni, S Novati, R Maserati, P Orsolini, M Vecchia, M Sciarra, E Asperges, M Colaneri, A Di Filippo, M Sambo, S Biscarini, M Lupi, S Roda, TC Pieri, I Gallazzi, M Sachs, P Valsecchi, S Perlini, C Alfano, M Bonzano, F Briganti, G Crescenzi, AG Falchi, R Guarnone, B Guglielmana, E Maggi, I Martino, P Pettenazza, S Pioli di Marco, F Quaglia, A Sabena, F Salinaro, F Speciale, I Zunino, M De Lorenzo, G Secco, L Dimitry, G Cappa, I Maisak, B Chiodi, M Sciarrini, B Barcella, F Resta, L Moroni, G Vezzoni, L Scattaglia, E Boscolo, C Zattera, MF Tassi, V Capozza, D Vignaroli, M Bazzini, G Iotti, F Mojoli, M Belliato, L Perotti, S Mongodi, G Tavazzi, G Marseglia, A Licari, I Brambilla, D Barbarini, A Bruno, P Cambieri, G Campanini, C. Cavanna, G Comolli, M Corbella, R Daturi, M Furione, P Marone, E Monzillo, S Paolucci, M Parea, E Percivalle, A Piralla, F Rovida, A Sarasini, M Zavattoni, G Adzasehoun, M Ardizzone, L Bellotti, V Brunco, E Cabano, G Casali, L Capella, D Devitis, L Dossena, G Frisco, G Garbagnoli, F Gardellini, A Girello, A Guerrizio, V Landini, C Lucchelli, V Maliardi, P Piemontese, S Pezzaia, M Premoli, C Rebuffa, C Zanello, J Bagnarino, F Bergami, A Bonetti, G Caneva, I Cassaniti, A Corcione, R Di Martino, A Di Napoli, A Ferrari, G Ferrari, L Fiorina, A Gallone, F Giardina, A Girardi, A Mercato, C Merla, F Novazzi, G Ratano, B Rossi, G Saveriaempillai, IM Sciabica, M Tallarita, E Vecchio Nepita, J Vitali, A Cerino, S Varchetta, B Oliviero, S Mantovani, D Mele, M Calvi, M Tizzoni, C Nicora, A Triarico, V Petronella, C Marena, A Muzzi, P Lago, S Cutti, V Novelli, F Comandatore, G BatistiBiffignandi, S Gaiarsa, M Rettani, and C Bandi
Subjects: Male, 0301 basic medicine, Human cytomegalovirus, Herpesvirus 4, Human, Epstein-Barr Virus Infections, viruses, Lymphocyte, COVID-19, EBV DNA, Lymphocyte subpopulation, Aged, Aged, 80 and over, CD8-Positive T-Lymphocytes, DNA, Viral, Female, Humans, Intensive Care Units, Killer Cells, Natural, Lymphocyte Count, Lymphocyte Subsets, Middle Aged, Opportunistic Infections, Real-Time Polymerase Chain Reaction, SARS-CoV-2, Viral Load, law.invention, 0302 clinical medicine, law, 80 and over, Killer Cells, Cytotoxic T cell, Viral, 030212 general & internal medicine, biology, musculoskeletal, neural, and ocular physiology, General Medicine, Intensive care unit, Infectious Diseases, medicine.anatomical_structure, Natural, Human herpesvirus 6, Human, Microbiology (medical), 030106 microbiology, macromolecular substances, Article, Virus, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, medicine, lcsh:RC109-216, Parvovirus, business.industry, Herpesvirus 4, DNA, biology.organism_classification, medicine.disease, nervous system, Immunology, business, CD8
Abstract: Highlights • EBV reactivation was observed in patients with COVID-19. • NK + and CD8 + T cell reduction was related to the severity of COVID-19 infection. • An increase of B cells in patients with severe symptoms was documented., Objectives The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. Methods A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Results Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p
Published: 2021
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20. The procoagulant pattern of patients with COVID‐19 acute respiratory distress syndrome

Author: Marco Dei Poli, Andrea Ballotta, Umberto Di Dedda, Marco Ranucci, Mara Falco, Giovanni Albano, Ekaterina Bayshnikova, Lorenzo Menicanti, and Marco Resta
Subjects: Male, medicine.medical_specialty, ARDS, medicine.drug_class, Pneumonia, Viral, Low molecular weight heparin, 030204 cardiovascular system & hematology, Fibrinogen, Gastroenterology, Betacoronavirus, 03 medical and health sciences, 0302 clinical medicine, Fibrinolytic Agents, Interquartile range, Internal medicine, D-dimer, medicine, Humans, Prospective Studies, Blood Coagulation, Pandemics, Aged, Blood coagulation test, SARS-CoV-2, business.industry, Antithrombin, Anticoagulants, COVID-19, Hematology, Blood Coagulation Disorders, Middle Aged, medicine.disease, COVID-19 Drug Treatment, Pulmonary embolism, Treatment Outcome, Host-Pathogen Interactions, Female, Blood Coagulation Tests, Coronavirus Infections, business, Biomarkers, medicine.drug
Abstract: Background Few observations exist with respect to the pro-coagulant profile of patients with COVID-19 acute respiratory distress syndrome (ARDS). Reports of thromboembolic complications are scarce but suggestive for a clinical relevance of the problem. Objectives Prospective observational study aimed to characterize the coagulation profile of COVID-19 ARDS patients with standard and viscoelastic coagulation tests and to evaluate their changes after establishment of an aggressive thromboprophylaxis. Methods Sixteen patients with COVID-19 ARDS received a complete coagulation profile at the admission in the intensive care unit. Ten patients were followed in the subsequent 7 days, after increasing the dose of low molecular weight heparin, antithrombin levels correction, and clopidogrel in selected cases. Results At baseline, the patients showed a pro-coagulant profile characterized by an increased clot strength (CS, median 55 hPa, 95% interquartile range 35-63), platelet contribution to CS (PCS, 43 hPa; interquartile range 24-45), fibrinogen contribution to CS (FCS, 12 hPa; interquartile range 6-13.5) elevated D-dimer levels (5.5 μg/mL, interquartile range 2.5-6.5), and hyperfibrinogenemia (794 mg/dL, interquartile range 583-933). Fibrinogen levels were associated (R2 = .506, P = .003) with interleukin-6 values. After increasing the thromboprophylaxis, there was a significant (P = .001) time-related decrease of fibrinogen levels, D-dimers (P = .017), CS (P = .013), PCS (P = .035), and FCS (P = .038). Conclusion The pro-coagulant pattern of these patients may justify the clinical reports of thromboembolic complications (pulmonary embolism) during the course of the disease. Further studies are needed to assess the best prophylaxis and treatment of this condition.
Published: 2020
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21. Altered Lipid Domains Facilitate Enhanced Pulmonary Vasoconstriction after Chronic Hypoxia

Author: Thomas C. Resta, Simin Yan, Jay S. Naik, Laura Weise-Cross, Charles E. Norton, Rosstin Ahmadian, Benjimen R. Walker, Michael L. Paffett, and Nikki L. Jernigan
Subjects: Male, 0301 basic medicine, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Caveolin 1, Clinical Biochemistry, Pulmonary Artery, Muscle, Smooth, Vascular, Membrane Potentials, Rats, Sprague-Dawley, Membrane Lipids, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Superoxides, Caveolae, Internal medicine, Hypoxic pulmonary vasoconstriction, medicine, Animals, Hypoxia, Molecular Biology, Superoxide, Cholesterol, Editorials, Cell Biology, Rats, ErbB Receptors, 030104 developmental biology, Endocrinology, 030228 respiratory system, chemistry, Vasoconstriction, Chronic Disease, Calcium, medicine.symptom, Signal transduction, Nicotinamide adenine dinucleotide phosphate, Signal Transduction
Abstract: Chronic hypoxia (CH) augments depolarization-induced pulmonary vasoconstriction through superoxide-dependent, Rho kinase-mediated Ca2+ sensitization. Nicotinamide adenine dinucleotide phosphate oxidase and EGFR (epidermal growth factor receptor) signaling contributes to this response. Caveolin-1 regulates the activity of a variety of proteins, including EGFR and nicotinamide adenine dinucleotide phosphate oxidase, and membrane cholesterol is an important regulator of caveolin-1 protein interactions. We hypothesized that derangement of these membrane lipid domain components augments depolarization-induced Ca2+ sensitization and resultant vasoconstriction after CH. Although exposure of rats to CH (4 wk, ∼380 mm Hg) did not alter caveolin-1 expression in intrapulmonary arteries or the incidence of caveolae in arterial smooth muscle, CH markedly reduced smooth muscle membrane cholesterol content as assessed by filipin fluorescence. Effects of CH on vasoreactivity and superoxide generation were examined using pressurized, Ca2+-permeabilized, endothelium-disrupted pulmonary arteries (∼150 μm inner diameter) from CH and control rats. Depolarizing concentrations of KCl evoked greater constriction in arteries from CH rats than in those obtained from control rats, and increased superoxide production as assessed by dihydroethidium fluorescence only in arteries from CH rats. Both cholesterol supplementation and the caveolin-1 scaffolding domain peptide antennapedia-Cav prevented these effects of CH, with each treatment restoring membrane cholesterol in CH arteries to control levels. Enhanced EGF-dependent vasoconstriction after CH similarly required reduced membrane cholesterol. However, these responses to CH were not associated with changes in EGFR expression or activity, suggesting that cholesterol regulates this signaling pathway downstream of EGFR. We conclude that alterations in membrane lipid domain signaling resulting from reduced cholesterol content facilitate enhanced depolarization- and EGF-induced pulmonary vasoconstriction after CH.
Published: 2020
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22. Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling

Author: Benjimen R. Walker, Nancy L. Kanagy, Lindsay M. Herbert, Simin Yan, Michelle A. Sands, Charles E. Norton, Joshua R. Sheak, Laura V. Gonzalez Bosc, Thomas C. Resta, Jessica B. Snow, Nikki L. Jernigan, and Laura Weise-Cross
Subjects: Male, 0301 basic medicine, Indoles, Potassium Channels, Vascular smooth muscle, Clinical Biochemistry, Pharmacology, Maleimides, 0302 clinical medicine, Hypoxic pulmonary vasoconstriction, Protein Interaction Mapping, Hypoxia, Cells, Cultured, Original Research, Membrane Potential, Mitochondrial, Chemistry, Nuclear Proteins, Intermittent hypoxia, Free Radical Scavengers, Potassium channel, Mesenteric Arteries, Mitochondria, Tetradecanoylphorbol Acetate, medicine.symptom, Signal Transduction, Pulmonary and Respiratory Medicine, Hypertension, Pulmonary, Myocytes, Smooth Muscle, Pulmonary Artery, Cyclic N-Oxides, 03 medical and health sciences, Sleep Apnea Syndromes, Right ventricular hypertrophy, Protein Kinase C beta, medicine, Animals, Humans, Rats, Wistar, Molecular Biology, Protein kinase C, Cell Biology, medicine.disease, Pulmonary hypertension, Rats, Cytoskeletal Proteins, 030104 developmental biology, 030228 respiratory system, Vasoconstriction, Spin Labels, Carrier Proteins, Reactive Oxygen Species
Abstract: Pulmonary vasoconstriction resulting from intermittent hypoxia (IH) contributes to pulmonary hypertension (pHTN) in patients with sleep apnea (SA), although the mechanisms involved remain poorly understood. Based on prior studies in patients with SA and animal models of SA, the objective of this study was to evaluate the role of PKCβ and mitochondrial reactive oxygen species (mitoROS) in mediating enhanced pulmonary vasoconstrictor reactivity after IH. We hypothesized that PKCβ mediates vasoconstriction through interaction with the scaffolding protein PICK1 (protein interacting with C kinase 1), activation of mitochondrial ATP-sensitive potassium channels (mitoK(ATP)), and stimulated production of mitoROS. We further hypothesized that this signaling axis mediates enhanced vasoconstriction and pHTN after IH. Rats were exposed to IH or sham conditions (7 h/d, 4 wk). Chronic oral administration of the antioxidant Tempol or the PKCβ inhibitor LY-333531 abolished IH-induced increases in right ventricular systolic pressure and right ventricular hypertrophy. Furthermore, scavengers of O(2)(−) or mitoROS prevented enhanced PKCβ-dependent vasoconstrictor reactivity to endothelin-1 in pulmonary arteries from IH rats. In addition, this PKCβ/mitoROS signaling pathway could be stimulated by the PKC activator PMA in pulmonary arteries from control rats, and in both rat and human pulmonary arterial smooth muscle cells. These responses to PMA were attenuated by inhibition of mitoK(ATP) or PICK1. Subcellular fractionation and proximity ligation assays further demonstrated that PKCβ acutely translocates to mitochondria upon stimulation and associates with PICK1. We conclude that a PKCβ/mitoROS signaling axis contributes to enhanced vasoconstriction and pHTN after IH. Furthermore, PKCβ mediates pulmonary vasoconstriction through interaction with PICK1, activation of mitoK(ATP), and subsequent mitoROS generation.
Published: 2020
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23. Detection of microsatellite instability-high (MSI-H) by liquid biopsy predicts robust and durable response to immunotherapy in patients with pancreatic cancer

Author: Sakti Chakrabarti, Leslie Bucheit, Jason Scott Starr, Racquel Innis-Shelton, Ardaman Shergill, Hiba Dada, Regina Resta, Stephanie Wagner, Naomi Fei, and Pashtoon Murtaza Kasi
Subjects: Pharmacology, Aged, 80 and over, Male, Cancer Research, Immunology, Liquid Biopsy, Middle Aged, Pancreatic Neoplasms, Oncology, Molecular Medicine, Immunology and Allergy, Humans, Immunologic Factors, Female, Microsatellite Instability, Immunotherapy, Aged, Retrospective Studies
Abstract: Clinical trials reporting the robust antitumor activity of immune checkpoint inhibitors (ICIs) in microsatellite instability-high (MSI-H) solid tumors have used tissue-based testing to determine the MSI-H status. This study assessed if MSI-H detected by a plasma-based circulating tumor DNA liquid biopsy test predicts robust response to ICI in patients with pancreatic ductal adenocarcinoma (PDAC). Retrospective analysis of patients with PDAC and MSI-H identified on Guardant360 from October 2018 to April 2021 was performed; clinical outcomes were submitted by treating providers. From 52 patients with PDAC +MSI-H, outcomes were available for 10 (19%) with a median age of 68 years (range: 56–82 years); the majority were male (80%) and had metastatic disease (80%). Nine of 10 patients were treated with ICI. Eight out of nine patients received single-agent pembrolizumab (8/9), while one received ipilimumab plus nivolumab. The overall response rate by Response Evaluation Criteria in Solid Tumors was 77% (7/9). The median progression-free survival and overall survival were not reached in this cohort. The median duration of treatment with ICI was 8 months (range: 1–24), and six out of seven responders continued to show response at the time of data cut-off after a median follow-up of 21 months (range: 11–33). Tissue-based MSI results were concordant with plasma-based G360 results in five of six patients (83%) who had tissue-based test results available, with G360 identifying one more patient with MSI-H than tissue testing. These results suggest that detecting MSI-H by a well-validated liquid biopsy test could predict a robust response to ICI in patients with PDAC. The use of liquid biopsy may expand the identification of PDAC patients with MSI-H tumors and enable treatment with ICI resulting in improved outcomes.
Published: 2022

24. Safety of hospital discharge before return of bowel function after elective colorectal surgery

Author: J Chapman, S, Blanco-Colino, R, Pérez-Ajates, S, O Anabitarte Bautista, Hodson, J, C Glasbey, J, Pata, F, Pellino, G, Sgrò, A, Soares, A, T van Elst, S Van Straten, Nepogodiev, D, Borakati, A, F Bath, M, H Yasin, I, Mclean, K, Arthur, T, Kovacevic, M, Delibegovic, S, Karamanliev, M, Swamad, M, Žebrák, R, Paramasivam, R, Martensen, A, M Larsen, H, Rädeker, L, E Frey, P, Kechagias, A, Venara, A, Duchalais, E, Ioannidis, A, Pasquali, S, Simioni, A, Farina, V, Podda, M, Lorenzon, L, Schaeff, V, Otto, A, Jakubauskas, M, R van Elst, T, Chu, M, Pvb, Fagan, I Wells, C, A Alagoa João, Juloski, J, Y El Kasmy El Kasmi, Espin-Basany, E, Clerc, D, Ciubotaru, C, Popescu, S, Yanishev, A, Lee, S, B Ozkan, B, A Yagız Sen, K Aktas, M, E Baki, B, Yüksek, B, Kamarajah, S, A Khaw, R, Mills, E, Goodson, R, Thakral, N, D Ablett, A, Adra, M, Kwek, I, M Khan, S, Quinn, P, R Manley, L, Badran, A, Ramjeeawon, A, Campbell, A, L Tan, H, S Rye, D, Rajaraman, N, G Norman, J, Vutipongsatorn, K, Solomou, G, Akhbari, M, Ali, A, Murray, V, M Baker, D, D Brandao, B, Stainer, B, Thavayogan, R, Jones, D, O Onafowokan, O, Gharooni, A, Dabab, N, Carlton-Carew, S, Kungwengwe, G, Gabriel, M, Sewart, E, C Shortland, T, Lawday, S, Pockney, P, Dawson, A, D Brumfitt, C, Stewart, P, B, Ng, K Luong, J, Ivanov, V, Borisova, A, Neykov, V, Kunčarová, K, Kološová, B, Antonova, T, Farkašová, M, L Harbjerg, J, Brandsborg, S, Brinck, S, D Kjaer, M, Mark-Christensen, A, P Unbehaun, K, Dalsgaard, P, D Lycke, K, Lenaoures, P, Brigand, C, Dumange, E, Gout, M, Moehwald, C, Prem, M, Alhalabi, O, Sliwinski, S, Krupp, J, Gablenz, E, Schmitzer, L, Kopp, A, Steinle, J, Gsenger, J, J Pohl, L, Riccardi, M, M Christodoulou, I, Konstantinidis, M, Machairas, N, Zoikas, A, Balalis, D, K Manatakis, D, L Aguilera, M, Marano, L, Fleres, F, Lovisetto, F, Sasia, D, Segalini, E, Pata, G, Lucchi, A, Sagnotta, A, Campagnaro, T, Petrelli, F, Gallo, G, Papandrea, M, Testa, V, Sinibaldi, G, F Di Candido, Colombo, F, Perrone, G, Aresu, S, Biancafarina, A, Canonico, G, Pagnanelli, M, Curletti, G, Bini, R, A de Manzoni Garberini, Impellizzeri, H, Cillara, N, Tutino, R, Picciariello, A, Coletta, D, Savino, G, Ferrara, F, Tamini, N, Talamo, G, Parini, D, Giamundo, P, A Lo Conte, Pagano, G, Ripetti, V, Pesce, A, Menduni, N, Giudicissi, R, Goldin, E, Rega, D, Belli, A, Andriola, V, Gordini, L, Foppa, C, Piccolo, G, Birindelli, A, Ferrari, C, Ballarini, Z, Tirelli, F, Milone, M, M De Rosa, S Pipitone Federico, N, Molteni, B, L Tilocca, P, Sancini, G, N Piozzi, G, Lauretta, A, Poillucci, G, Mulas, S, Simcikas, D, Portelli, L, Jwm van Wijnbergen, L Dinger, T, Sfh Ten Doesschate, Ashm van Dalen, D van den Bos, D, Hansmann, M, J Medina Feliz, Z Kuiper, S, Abdulrahman, Z, R Pruijssers, S, Farik, S, M Elliott, B, P Geneta, V, Wilton, S, Kandelaki, H, L Peng, S, Campbell, S, K Lim, Y, S Yassaie, S, Murray, M, Haran, C, Tan, J, Castro, J, Laranjeira, A, Catarino, S, Neves-Marques, C, G Correia, J, N Vieira, B, C Quintela, A, L Serra, M, Maciel, J, Cunha, M, J Aparício, D, Neves, J, Azevedo, J, Romano, M, Eiró, F, Romano, J, Monteiro, C, Claro, M, R Almeida, M, Peyroteo, M, D Machado, N, Capote, H, Ferreira, M, Sousa, X, Devesa, H, Cavadas, D, Guerreiro, I, Costa, M, Rosete, M, Salman, M, English, C, Mohammed, N, Litvin, A, V Ćuk, V, Mészárosová, K, Jaich, R, H De Lima, Brooks, S, Marx, M, M Nshalati Salvation, J García Cardo, Mora-Guzmán, I, J Sancho Muriel, U de Andres Olabarria, Muriel, P, C Jimenez Viñas, Alconchel, F, O Esteban Sinovas, C Fairén Oro, M López Otero, S Figueroa Jiménez, O Claramonte Bellmunt, J Martínez Caballero, Rubio-Pérez, I, M Aguilar-Martínez, M, J Segura-Sampedro, J, C Olmedo Moreno, D Negre Parra, M Estévez Diz, A, Martín-Balbuena, R, C Bustamante Recuenco, E Licardie Bolaños, R, Fernández, P, A Díaz Padillo, Forero-Torres, A, I Alberdi San Román, H Salvador Rosés, M Merayo Álvarez, P Villarejo Campos, C Lopes Moreira, C, P Uriol Peralta, M Serrano Navidad, Ripollés-Melchor, J, Garcea, A, H Gómez Facundo, P Troncoso Pereira, V Pérez Guarinos, C, Blaser, B, Piazza, G, Gagliardi, B, Serin, H, S Yurdaor, S, Arslan, E, Kopac, O, Uyanik, A, B Ozmen, B, Tiftik, E, Aksoy, B, Yalcinkaya, A, Ozoglu, F, D Kocer, M, Bilicen, G, N Cinar, E, Uslu, Ö, Kaya, Y, Demirci, K, Wong, J, Mmh, Farhan-Alanie, Suresh, G, Asif, A, J Finch, B, Bhahirathan, Y, Herron, J, Z Yi Tew, Obukofe, R, Russell, C, Suchett-Kay, I, Netke, T, Williams, L, Kisiel, A, Y Liu, F, Claireaux, H, James, P, Mondal, A, Kalderon, R, H Nadama, H, Al-Saraff, Z, Jph, Tam, Powell-Chandler, A, Wood, F, Rebecca, Gorgievska, Ragavoodoo, A, Thakrar, C, Rojoa, D, Palmer, C, Davidson, K, Giacci, L, Hale, J, W Gan, F, Makin-Taylor, R, Y Hey, C, Toh, C, M Findlay, J, Griffiths, N, Ganesananthan, S, Jasionowska, S, Poustie, M, Wong, C, Turner, T, Pyc, W, Sloper, W, Warner, C, Coey, J, Mason, D, Sait, S, Kowal, M, Owen, M, Saiyed, A, Ashworth, I, Akbari, K, Curran, M, Martin, P, Parker, D, Kwok, K, Lye, C, Ghaly, M, Sammour, T, Lewis, D, Mundasad, R, Wilkes, A, Ctercteko, G, Maslyankov, S, Dimov, R, Iliev, S, Dimitrov, D, Marek, F, Örhalmi, J, Skalický, P, Skalický, T, Chrz, K, Christensen, P, Worsøe, J, S Kristensen, E, J Emmertsen, K, S Loeve, U, L Mihaljevic, A, Herrle, F, M Konstantinidis, K, Korkolis, D, Karanikas, I, Vincenti, L, Anania, G, Borghi, F, Agresta, F, Maretto, I, Parisi, A, Bucci, L, G De Palma, Guglielmi, A, Cucinotta, E, F La Torre, Cianchi, F, Guerrieri, M, Trompetto, M, Persiani, R, Micheletto, G, Delrio, P, Cantafio, S, Ronconi, M, Pag, Bisagni, M De Prizio, Franceschi, A, Galleano, R, Cavallini, M, Brescia, A, D'Ambra, L, Benevento, A, Niolu, P, Calgaro, M, Colangelo, E, Grottola, T, F Altomare, D, Puleo, S, Salamone, G, Pietrabissa, A, Poggioli, G, Erdas, E, Ottonello, R, Tonini, V, Selvaggi, F, Sammarco, G, Ceccarelli, G, C De Nisco, Surgo, D, Taglietti, L, Ozolins, A, Sivinš, A, Poskus, T, Psaila, J, A Bemelman, W, J Graat, L, Langenhoff, B, Bpl, Wijnhoven, Ahw van de Ven, Poelman, M, Lps, Stassen, Slooter, G, Yiz, Acherman, Hoff, C, F Gerhards, M, Mwj, Stommel, J Hazebroek, E, Aaw van Geloven, A Schasfoort, R, L van Leeuwen, B, B Tuynman, J, Mwa van Tilburg, G Boerma, E, Sharma, P, Jenkins, B, P Bissett, I, Herd, A, Gordon, A, Vernon, D, Omundsen, M, J, Ly, Reddy, A, Bonnet, G, Harmston, C, Morales, M, Francisco, V, Costa, S, Manso, A, Amorim, E, Pereira, J, Cardoso, J, Ourô, S, Caratão, M, Nascimento, C, B Ribeiro da Silva, Taranu, V, Dias, R, Mendes, J, Allen, M, Silva, A, Carlos, S, Barbosa, E, Carneiro, C, Ramos, L, Lencastre, L, Martins, R, Silva-Vaz, P, F Ridgway, P, A McNamara, D, Cahill, R, Hogan, A, Larkin, J, R O'Connell, P, Negoi, I, Abelevich, A, M Ćuk, V, Vician, M, Ede, C, Sardiwalla, I, Mulira, S, Montwedi, D, Oyomno, M, Sabia, D, V Portugal Porras, Vigorita, V, G Sanz Ortega, García, J, A Espí Macías, F Blanco Antona, A Luján Mompeán, J, S Salvans Ruiz, Villarejo-Campos, P, M Romero Simó, Sánchez-Guillén, L, M Jiménez-Gómez, L, A 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Cuming, T, Saha, A, Chung, L, Pitt, J, Davis, P, Jones, O, Taylor, M, Bhargava, A, Haji, A, Watson, N, Bloom, I, Singh, B, Norwood, M, Gurjar, S, Stylianides, N, Mirza, S, Evans, M, Williams, G, Patil, P, Hernon, J, Finch, G, Green, S, Chapple, K, Fafemi, O, Warusavitarne, J, Samee, A, Carden, C, Ong, L, Verma, K, Joseph, A, Rawat, N, Pinkney, T, Oke, O, Glen, P, Maxwell-Armstrong, C, Oliphant, R, Garner, J, J Moug, S, Middleton, S, N Lund, J, J Smart, N, Osborn, G, Moore, T, Raymond, T, H Knowles, C, S Hany, T, Clarke, R, Khera, G, Brady, R, Sellahewa, C, Mason, C, Torrance, A, Lasithiotakis, K, Knight, J, Pullybank, A, Ainsworth, P, Reid, F, Ramwell, A, Maslekar, S, George, R, Skull, A, Holtham, S, Muhammad, K, Lal, R, Varcada, M, M Smith, F, Howlader, M, Defriend, D, Kirk, S, Richards, T, Evans, C, M Borg, C, Telford, K, Sarfraz, N, Busby, K, Hollingshead, J, Speake, D, Pawa, N, West, D, Chadwick, M, Komolafe, O, Richardson, S, Thornton, M, Goede, A, Osborne, C, Bandyopadhyay, D, Foong, J, J Lee, Y, Liebenberg, P, Mijalkov, D, Wells, A, Bull, N, Ajmera, A, Warburton, T, Morgan, S, Mahmoud, A, Schachtel, M, Mikhail, B, Fomin, I, Mekaeil, B, Taylor, N, Stevenson, C, Drane, A, Pahalawatta, U, T Lai, L, Debiasio, A, Hjs, Jun, Hengpoonthana, R, M Mendis, D, M Robb, P, J Lee, H, Aab, Wyche, T Davis, L, Chrimes, A, Agarwal, A, Zhao, J, Williams, S, Jmsn, Jayalath, Khor, S, Muddasani, T, Childs, S, Ridgway, S, Nda, Blefari, Tam, H, Puchalski, N, Ngai, C, Mackenzie, J, Johnson, N, Holmes, M, Zuzek, R, Saluja, T, Gould, T, K Goh, Y, Selvaraj, T, Z Beh, Y, N Dudi-Venkata, N, Horne, D, L Borrow, J, Campbell, C, Cousins, G, Jackson, L, Maheepala, K, Zhao, S, Holden, E, Tutt, L, Thompson, B, Collins, H, Louie, F, Buckland, B, Smith, D, Chong, C, H Chua, T, Nayak, C, Redmond, J, R Tan, R, Gramlick, M, S Teh, J, Y Ng, S, Britten-Jones, P, R Mohd Rosli, Hdv, Pham, Jegathees, T, Smcj, Coulter-Nile, P Gosselink, M, L Wang, Y, Maciaszek, M, S Chrapko, P, Nair, A, Thirugnanasundralingam, V, Muir, K, Salibasic, M, Pavlov, V, Paycheva, T, Lyulenina, E, Kolev, N, Nguen, D, Mitkov, Y, Mitkov, E, Vladova, P, Dimitrov, V, Hussain, M, Gabarski, A, Ivanov, T, Yotsov, T, Ilieva, I, Akisheva, A, Shoshkova, M, Nawaz, E, Feradova, H, Mladenov, T, Jozaf, V, Klail, T, Pös, M, Adel, A, Sotona, O, Bartoš, M, Amjad, T, Malý, O, Berec, S, Hanušová, M, Hurný, M, Riško, J, Ludvik, M, Stercz, M, Treskoň, R, Pospíšil, M, Hlaváčová, L, Tomanová, D, Chodora, S, Houdek, O, Novický, R, Sobotková, K, Cha, S, K Šuta Kimle, Jirankova, K, Bujda, M, Paclík, A, Trap, A, Jürgens-Lahnstein, J, Storm, M, Damgaard, I, Olawi, F, Ehlern, F, Raos, M, P Kristensen, F, Bønnerup, K, Amiri, S, Enevoldsen, M, J Højgaard Pedersen, N Jepsen, B, K Hillgaard, T, B Erichsen, S, V Nielsen, C, P Madsen, C, Bjerke, J, D Skejø, C, R Aabling, R, S Sørensen, J, Turunen, A, Katunin, J, Niskakangas, M, Vignaud, T, Frey, S, Ricolleau, C, Chanut, F, Magnin, J, Seiboldt, T, Beck, L, Zamzow, K, Betge, F, Poncelet, A, Truant, M, Hauschild, H, Neugebauer, N, Schöning, L, S Cs Simon, Galata, C, Karampinis, I, Thäwel, T, M Seckler, A, Kerem, C, Durdevic, S, Antonakopoulos, F, Mathioulaki, A, Chrysoheris, P, G Athanasopoulos, P, Kalles, V, Spyrou, I, Barkolias, C, Paspala, A, Papaconstantinou, D, Spartalis, E, Arkadopoulos, N, Prodromidou, A, Garoufalia, Z, Mendez, D, Rosales, J, Flores, M, Garcia, M, Garcia, A, Noriega, Z, Torselli, D, Rodriguez, J, Lafranceschina, S, Artioli, E, Giaccari, S, Nevoso, V, Schimera, A, Marino, S, Geretto, P, Pellegrino, L, Borghi, B, Marano, A, Corino, C, Cannata, G, C Giuffrida, M, Landra, F, Ongaro, D, Baronio, G, Raimondo, S, Casiraghi, S, Salvadori, R, Finotti, E, Ciccioli, E, Galgano, A, Zuin, M, Bettella, A, Barina, A, Vendramin, E, Palano, G, Schiavone, D, A Di Cintio, Gemini, A, Trastulli, S, M De Luca, Desiderio, J, Gubbiotti, F, Cigognini, M, Zaffaroni, G, Maffioli, A, Colombo, S, Bondurri, A, Sampietro, G, Foschi, D, Manigrasso, M, Danzi, M, Amato, 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Kenny R., Kan P., Lee D.E., Khosla S., Samake M., Shaban F., Aftab R., Gough M., Woodburn B., Vayalapra S., McMurrugh K., Jimulia D., Deol S., Pike S., Embury-Young Y., Patel M., Kilgallon E., Keating R., Walsh A., Khan H., Logue G., Orekoya M., Alasmar M., Charalambides M., Llavall A.C., Williamson E., Bharwada Y., Zearmal S., Evans H., Panikkar M., de la Cruz G., Caplan J., Ruparelia A., Tanvir T., Soare C., Pang Y.L., Trotter J., Zaidi A., Thakrar V., Pulickal P., Ahmed H., Parnell J., Lennock S., Ford V., Brignall R., O'Neill D., Hanna R., Kane R., Nicola M., Rajput K., Xiao Y., Michael S., Wright E., Juniper S., Thompson E., Hoskyns L., Kanitkar A., Ross C., Unsworth A., Rshaidat H., Demarre K., Chiang A., Bareh A., van Dellen J., Faqihinejad C., Gadhvi A., Grant R., Lewsey J., Morris A., Martin H., McClarty C., Sanyal S., Alsaif A., Palkhi A., Bhopal S., Burford C., Huq T., Irwin E., Matthews L., Ngu W.S., Hosfield T., Muneeb F., Page O., Zeb E., Al-Azzawi A., McIntosh J., Vucicevic A., Hughes M., Brooks L., Fanibi B., Dixon M., Njoku P., Morris D., Jobson J., Chowdhury H., Joseph N., Zulkefley N., Hunt G., Christodoulou T., Wright O., Soman S., Jamal M., Beqiri S., Borgas P., Christie S., Pereira F., Browne S., Yiu J., Dworkin A., Brayley J., Palmer A., Charalambos M., Jones C.S., Toner S., Cowden R., Lee L., Nicol P., Holman O., Imtiaz M., Albert V., Leung S.P., Erotocritou M., Stroud R., Wilkin R., Thomson W., Mackee L., Kler A., Reynolds L., Mohamed S.H., Majeed Y., Fakim B., Jones A., Liversedge G., Carrington Z., Windebank J., Izzarina A., Akbani U., Craven J., Aldarragi A., Harding S., Millward A., Bedford M., Gopalan V., Midgen A., Khadka P., Cheng O., Taneja S., Manobharath N., Kok J.Y., Lim D.W.E., Buick T., Boland M., Piya S., Devlin R., Fairfield C.J., George R.J., Rahi M., Zaman S., Hajiev S., Ross T., Crisp E., Thompson C., Charalambous A., Hollywood J.L., Hammond R.F.L., Matthews J., Mendonca V., Spinty J., Khan K., Cheng J., Glynn N., 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M., Frade S., Senhorinho R., Peixoto R., Alves-Vale C., Lamas M., O'Connor D.B., Hoo M., Gopaul A., Scanlon K., O'Dwyer N., Jovanovic M., Panyko A., Van Vuuren S., Centeno A., Bernado I.R., Senorans M.P.G., Amor L.G., Ramirez A.C., Abrisqueta J., Gomez M.E., Arroyo A., Cerdan C., Romeu N.G., Enriquez-Navascues J.M., Collado-Roura F., Curchod P., Gaspar S., Imadalou L., Mutlu D., Akyol C., Uygur F.A., Eray I.C., Biyiklioglu O., Cetin M.F., Isik A.E., Karip B., Dogan H., Sarigul L., Tunc E., Aydin T., Bodur S., Karabulut K., Francis A.A., Al-hadithi A., Lau I.S.F., Smith E., Mahapatra S., McAuliffe O., Imam L., Akram B., Hossaini S., Davies R., Ko M., Collins J., Pandya A., Reilly S., Archer J., Livie J., Chaudhry F.A., Ntakomyti E., Diallo R., Bylinski T., Wright J., Masiha E., Tung J., Shirazi B., Neilson A., Epton S., Patel N., Trussell S., Couldrey A., Donnelly C., Eftychiou S., Salman M., Skejo, Licari L., Men, Chapman, S. 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Z., Abdulrahman, Z., Pruijssers, S. R., Geneta, V. P., Chu, M., Wilton, S., Kandelaki, H., Peng, S. L., Campbell, S., Lim, Y. K., Yassaie, S. S., Murray, M., Haran, C., Tan, J., Castro, J., Laranjeira, A., Catarino, S., Neves-Marques, C., Correia, J. G., Vieira, B. N., Quintela, A. C., Serra, M. L., Maciel, J., Cunha, M., Aparicio, D. J., Neves, J., Azevedo, J., Romano, M., Eiro, F., Romano, J., Monteiro, C., Claro, M., Capote, H., Ferreira, M., Sousa, X., Peyroteo, M., Machado, N. D., Guerreiro, I., Costa, M., Rosete, M., Salman, M., English, C., Mohammed, N., Litvin, A., Cuk, V. V., Meszarosova, K., Jaich, R., De Lima, H., Brooks, S., Marx, M., Salvation, M. N., Cardo, J. G., Mora-Guzman, I., Muriel, J. S., de Andres Olabarria, U., Muriel, P., Vinas, C. J., Alconchel, F., Sinovas, O. E., Oro, C. F., Otero, M. L., Jimenez, S. F., Bellmunt, O. C., Caballero, J. M., Rubio-Perez, I., Aguilar-Martinez, M. M., Segura-Sampedro, J. J., Moreno, C. O., Parra, D. N., Diz, A. M. 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M., Griffiths, N., Ganesananthan, S., Jasionowska, S., Poustie, M., Wong, C., Turner, T., Pyc, W., Sloper, W., Warner, C., Coey, J., Mason, D., Sait, S., Kowal, M., Owen, M., Saiyed, A., Ashworth, I., Akbari, K., Curran, M., Martin, P., Parker, D., Kwok, K., Lye, C., Ghaly, M., Sammour, T., Lewis, D., Mundasad, R., Wilkes, A., Ctercteko, G., Maslyankov, S., Dimov, R., Iliev, S., Dimitrov, D., Marek, F., Orhalmi, J., Skalicky, P., Skalicky, T., Chrz, K., Christensen, P., Worsoe, J., Kristensen, E. S., Emmertsen, K. J., Loeve, U. S., Mihaljevic, A. L., Herrle, F., Konstantinidis, K. M., Korkolis, D., Karanikas, I., Vincenti, L., Anania, G., Borghi, F., Agresta, F., Maretto, I., Parisi, A., Bucci, L., De Palma, G., Guglielmi, A., Cucinotta, E., La Torre, F., Cianchi, F., Guerrieri, M., Trompetto, M., Persiani, R., Micheletto, G., Delrio, P., Cantafio, S., Ronconi, M., Bisagni, P. A. 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G., Hubner, M., Petermann, D., Sauvain, M. O., Ozben, V., Gecim, I. E., Disci, E., Rencuzogullari, A., Kurt, A., Bisgin, T., Pehlivan, M., Isik, A., Onur, E., Leventoglu, S., Keskin, M., Guner, A., Sahin, S. T., Ozbalci, G. S., Pergel, A., Albayrak, D., Bruce, D., Fearnhead, N., Arthur, J., Harron, M., Beattie, G., Titu, L., Saunders, M., Phillips, J., Dindyal, S., Cresswell, B., Gercek, Y., Lee, J., Linn, T., Faulkner, G., Lockwood, S., Rees, J., Charalabopoulos, A., Campbell, B., Kontovounisios, C., Amarnath, T., Johnson, M., Epanomeritakis, E., Vigs, S., Nastro, P., Gilliam, A., Smolarek, S., Wilson, T., Orbell, J., Mcintyre, R., Agarwal, T., Hainsworth, P., Patel, P., Vijay, J., Liu, B., Rao, P. D., Roxburgh, C., Vipond, M., Youssef, H., Thorn, C., Schizas, A., Denley, S., Bowley, D., Das, K., Cuming, T., Saha, A., Chung, L., Pitt, J., Davis, P., Jones, O., Taylor, M., Bhargava, A., Haji, A., Watson, N., Bloom, I., Singh, B., Norwood, M., Gurjar, S., Stylianides, N., Mirza, S., Evans, M., Williams, G., Patil, P., Hernon, J., Finch, G., Green, S., Chapple, K., Fafemi, O., Warusavitarne, J., Samee, A., Carden, C., Ong, L., Verma, K., Joseph, A., Rawat, N., Pinkney, T., Oke, O., Glen, P., Maxwell-Armstrong, C., Oliphant, R., Garner, J., Moug, S. J., Middleton, S., Lund, J. N., Smart, N. J., Osborn, G., Moore, T., Raymond, T., Knowles, C. H., Hany, T. S., Clarke, R., Khera, G., Brady, R., Sellahewa, C., Mason, C., Torrance, A., Lasithiotakis, K., Knight, J., Pullybank, A., Ainsworth, P., Reid, F., Ramwell, A., Maslekar, S., George, R., Skull, A., Holtham, S., Muhammad, K., Lal, R., Varcada, M., Smith, F. M., Howlader, M., Defriend, D., Kirk, S., Richards, T., Evans, C., Borg, C. 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W., Gabbas, G., Marziali, I., Mazzocato, S., Vergari, R., Piazzai, F., Kubolli, I., Aggiusti, A., Paolucci, A., Ortenzi, M., Olivieri, M., Belluco, C., Antona, A. D., Basso, S., Morino, M., Mistrangelo, M., Clerico, G., De Santi, G., Bitonti, M. F., Frattalone, M., Fico, V., Santullo, F., Belia, F., Spinelli, A., Marco, M., Bevilacqua, M., Tringali, D., Bevilacqua, E., Panizzo, V., La Manna, V., Migliore, G., Aversano, A., Bucci, A. F., Marino, F., Carbone, F., Incollingo, P., Romano, F. M., Zalla, T., Baraghini, M., Romoli, L., Calussi, M., Vellei, S., Genzano, C., Feroci, F., Vita, M., Barberis, A., Serra, D., Grassia, M., Romelli, M., Ruggiero, S., Percassi, A., Piccinini, A. 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X., Peckham, H., Yeoh, T., Yong, S. Q., Chen, J. Y., Siva, S., Sam, Z. H., Gilani, M., Goh, Y. N., Muthukumar, M. G., Phillips, S., Tjoakarfa, J., Giri, A., Suresan, S., Thomas, P., Johnson, T. A., Williams, R. I., Rashid, A., Kushairi, A., Rais, A., James, A., Bugelli, M., Chechelnitskaya, Y., Sandhu, N., Tandon, R., Gray, M., Kumar, A., Ciurleo, C., Nyamali, I., Hiremath, S., Sinha, S., Chowdhary, M., Bradley, E., Mctiernan, M., Macdonald, S., Sharkey, S., Mclaughlin, N., Amey, C., Kraria, L., Skan, O., Kind, C., Tupper, P., Van Rhee, C., Honeyman, S. I., Menon, G., Jegatheeswaran, L., Madhavan, A., Warne, M., Malcolm, F. L., Lessware, T., Wilkerson, H. T., Chatterjee-Woolman, S., Yoong, A., Ahmed, W. U. R., Longshaw, A., Flannery, O., Green, R., Leaning, M., Cragg, J., Sharriff, H., Doherty, C., Kwan, K. W. L., Sanders-Crook, L., Bhatia, S., Eames, S., Lewis, F., Kirupananthan, P., Boh, Z. Y., Dass, S., Soma, A., Newton, A., Hill, M., Shafiq, Y., Brkljac, M., Boyce, L., English, W. J., Lam, S., Chipeta, C., Jain, C., Garofalidou, T., Novotny, S. A., Locke, S., Bowman, C., Begaj, A., Murphy, C., Radcliffe, K., Chong, J. T., Jeffrey, E., Chaudhury, N., Rajendran, K., Akbar, Z., Walters, B., Kulendrarajah, B., Tran, N., Shrestha, S., Parmar, S., Gallagher, C., Hennessy, L., Pentti, E., Badhrinarayanan, S., Fung, A., Mansoor, M., Kenny, R., Kan, P., Lee, D. E., Khosla, S., Samake, M., Shaban, F., Aftab, R., Gough, M., Woodburn, B., Vayalapra, S., Mcmurrugh, K., Jimulia, D., Deol, S., Pike, S., Embury-Young, Y., Patel, M., Kilgallon, E., Keating, R., Walsh, A., Khan, H., Logue, G., Orekoya, M., Alasmar, M., Charalambides, M., Llavall, A. C., Williamson, E., Bharwada, Y., Zearmal, S., Evans, H., Panikkar, M., de la Cruz, G., Caplan, J., Ruparelia, A., Tanvir, T., Soare, C., Pang, Y. L., Trotter, J., Zaidi, A., Thakrar, V., Pulickal, P., Ahmed, H., Parnell, J., Lennock, S., Ford, V., Brignall, R., O'Neill, D., Hanna, R., Kane, R., Nicola, M., Rajput, K., Xiao, Y., Michael, S., Wright, E., Juniper, S., Thompson, E., Hoskyns, L., Kanitkar, A., Ross, C., Unsworth, A., Rshaidat, H., Demarre, K., Chiang, A., Bareh, A., van Dellen, J., Faqihinejad, C., Gadhvi, A., Grant, R., Lewsey, J., Morris, A., Martin, H., Mcclarty, C., Sanyal, S., Alsaif, A., Palkhi, A., Bhopal, S., Burford, C., Huq, T., Irwin, E., Matthews, L., Ngu, W. S., Hosfield, T., Muneeb, F., Page, O., Zeb, E., Al-Azzawi, A., Mcintosh, J., Vucicevic, A., Hughes, M., Brooks, L., Fanibi, B., Dixon, M., Njoku, P., Morris, D., Jobson, J., Chowdhury, H., Joseph, N., Zulkefley, N., Hunt, G., Christodoulou, T., Wright, O., Soman, S., Jamal, M., Beqiri, S., Borgas, P., Christie, S., Pereira, F., Browne, S., Yiu, J., Dworkin, A., Brayley, J., Palmer, A., Charalambos, M., Jones, C. S., Toner, S., Cowden, R., Lee, L., Nicol, P., Holman, O., Imtiaz, M., Albert, V., Leung, S. P., Erotocritou, M., Stroud, R., Wilkin, R., Thomson, W., Mackee, L., Kler, A., Reynolds, L., Mohamed, S. H., Majeed, Y., Fakim, B., Jones, A., Liversedge, G., Carrington, Z., Windebank, J., Izzarina, A., Akbani, U., Craven, J., Aldarragi, A., Harding, S., Millward, A., Bedford, M., Gopalan, V., Midgen, A., Khadka, P., Cheng, O., Taneja, S., Manobharath, N., Kok, J. Y., Lim, D. W. E., Buick, T., Boland, M., Piya, S., Devlin, R., Fairfield, C. J., George, R. J., Rahi, M., Zaman, S., Hajiev, S., Ross, T., Crisp, E., Thompson, C., Charalambous, A., Hollywood, J. L., Hammond, R. F. L., Matthews, J., Mendonca, V., Spinty, J., Khan, K., Cheng, J., Glynn, N., Muhammad, U., Khan, M., Anderson, L., Mccormack, K., Mak, J., Patrawala, S., Milinkovic, N., Schofield, R., Chauhan, M., Hartley, L., Hind, J., Nelson, L., Ratnasingham, D., Whitehead, T., Dimitriadis, S., Marshall, K., Flint, E. J., Horner, C., Heybourne, A., Morgan, H., Wickstone, C., Panagiotou, D., O'Connell, E., Dean, K., Iqbal, R., Walsh, L., Yu, N., Rana, N., Massie, E., Ng, J., Jung, M., Lee, Y. D., Harris, M., White, S., Boev, B., Tonchev, P., Prochazka, V., Risko, J., Skalicky, A., Ravn, S., Ojakaar, A., Dorr-Harim, C., Koutserimpas, C., Giraudo, G., Armellini, A., Ruzzenente, A., Mazzeo, C., De Padua, C., Luc, A. R., Maroli, A., Vitali, M., Gusai, G. P., Quattromani, R., Virgilio, E., Berti, S., Di Mola, F. F., Papagni, V., Tuminello, F., Vittori, L., Longheu, A., Loche, G. A., Braccio, B., De Luca, E., Resta, G., Ancans, G., Tamosiunas, A., Petrulionis, M., Abdulrahman, N., van de Pas, K. G. H., Thomas, G., Brandsma, A. M., Davids, J., Rottier, S. J., de Roy van Zuidewijn, D., Hawkins, R., Ong, H. I., Li, Y., Desmond, B., Winstanley, J., Martins, M., Americano, M., Frade, S., Senhorinho, R., Peixoto, R., Alves-Vale, C., Lamas, M., O'Connor, D. B., Hoo, M., Gopaul, A., Scanlon, K., O'Dwyer, N., Jovanovic, M., Panyko, A., Van Vuuren, S., Centeno, A., Bernado, I. R., Senorans, M. P. G., Amor, L. G., Ramirez, A. C., Abrisqueta, J., Gomez, M. E., Arroyo, A., Cerdan, C., Romeu, N. G., Enriquez-Navascues, J. M., Collado-Roura, F., Curchod, P., Gaspar, S., Imadalou, L., Mutlu, D., Akyol, C., Uygur, F. A., Eray, I. C., Biyiklioglu, O., Cetin, M. F., Isik, A. E., Karip, B., Dogan, H., Sarigul, L., Tunc, E., Aydin, T., Bodur, S., Karabulut, K., Francis, A. A., Al-hadithi, A., Lau, I. S. F., Smith, E., Mahapatra, S., Mcauliffe, O., Imam, L., Akram, B., Hossaini, S., Davies, R., Ko, M., Collins, J., Pandya, A., Reilly, S., Archer, J., Livie, J., Chaudhry, F. A., Ntakomyti, E., Diallo, R., Bylinski, T., Wright, J., Masiha, E., Tung, J., Shirazi, B., Neilson, A., Epton, S., Patel, N., Trussell, S., Couldrey, A., and Donnelly, C.
Subjects: operative, Male, postoperative discharge, Postoperative Complications, 0302 clinical medicine, Colostomy, CRITERIA, Prospective Studies, Bowel function, Colectomy, IMAGINE, stoma, intestines, Proctectomy, Ileostomy, digestive, oral, and skin physiology, patient discharge, colorectal surgery, hospital discharge, bowel function, RECOVERY, Middle Aged, adult, colostomy, elective surgical procedures, female, follow-up studies, humans, ileostomy, ileus, male, middle aged, multivariate analysis, patient readmission, patient safety, postoperative complications, prospective studies, recovery of function, colectomy, proctectomy, Patient Discharge, Colorectal surgery, ileus - - discharge - bowel function - elective - colorectal surgery, Elective Surgical Procedures, 030220 oncology & carcinogenesis, Female, 030211 gastroenterology & hepatology, Patient Safety, Cohort study, safety, Adult, medicine.medical_specialty, Ileus, Patient Readmission, defecation, 03 medical and health sciences, medicine, Hospital discharge, Humans, colorectal surgery, colorectal resection, Colorectal resection, defecation, postoperative complications, colorectal surgery, intestines, patient discharge, patient readmission, safety, surgical procedures, operative, colorectal resection, ileus, business.industry, Recovery of Function, Odds ratio, medicine.disease, digestive system diseases, surgical procedures, Surgery, Multivariate Analysis, Defecation, business, Follow-Up Studies
Abstract: Ileus is common after colorectal surgery and is associated with an increased risk of postoperative complications. Identifying features of normal bowel recovery and the appropriateness for hospital discharge is challenging. This study explored the safety of hospital discharge before the return of bowel function.A prospective, multicentre cohort study was undertaken across an international collaborative network. Adult patients undergoing elective colorectal resection between January and April 2018 were included. The main outcome of interest was readmission to hospital within 30 days of surgery. The impact of discharge timing according to the return of bowel function was explored using multivariable regression analysis. Other outcomes were postoperative complications within 30 days of surgery, measured using the Clavien-Dindo classification system.A total of 3288 patients were included in the analysis, of whom 301 (9·2 per cent) were discharged before the return of bowel function. The median duration of hospital stay for patients discharged before and after return of bowel function was 5 (i.q.r. 4-7) and 7 (6-8) days respectively (P 0·001). There were no significant differences in rates of readmission between these groups (6·6 versus 8·0 per cent; P = 0·499), and this remained the case after multivariable adjustment for baseline differences (odds ratio 0·90, 95 per cent c.i. 0·55 to 1·46; P = 0·659). Rates of postoperative complications were also similar in those discharged before versus after return of bowel function (minor: 34·7 versus 39·5 per cent; major 3·3 versus 3·4 per cent; P = 0·110).Discharge before return of bowel function after elective colorectal surgery appears to be safe in appropriately selected patients.El íleo es frecuente tras la cirugía colorrectal y se asocia con un aumento del riesgo de las complicaciones postoperatorias. Identificar las características de una recuperación normal del tránsito y la idoneidad para el alta hospitalaria es una tarea difícil. Este estudio examinó la seguridad del alta hospitalaria antes del restablecimiento de la función intestinal. MÉTODOS: Se llevó a cabo un estudio de cohortes prospectivo y multicéntrico a través de una red de colaboración internacional. Se incluyeron pacientes adultos sometidos a resección electiva colorrectal entre enero y abril 2018. El objetivo principal fue el reingreso hospitalario durante los 30 primeros días tras la intervención. Se utilizó un análisis de regresión multivariable para evaluar el impacto del momento del alta en función del restablecimiento de la función intestinal. El objetivo secundario fue las complicaciones postoperatorias en los primeros 30 días después de la cirugía, valoradas mediante el sistema de clasificación de Clavien-Dindo.Se incluyeron en el análisis 3.288 pacientes, de los cuales 301 (9,2%) fueron dados de alta antes del restablecimiento de la función intestinal. La mediana de la estancia hospitalaria de los pacientes dados de alta antes y después del restablecimiento de la función intestinal fue 5 (rango intercuartílico: 4-7) y 7 (6-8) días, respectivamente (P 0,001). No hubo diferencias significativas en las tasas de reingreso entre estos grupos (6,6% versus 8,0%; P = 0,499), y este resultado se mantuvo en el análisis multivariable tras el ajuste por diferencias basales (razón de opotunidades, odds ratio, OR 0,90; i.c. del 95% 0,55-1,46, P = 0,659). Las tasas de complicaciones postoperarorias fueron similares en aquellos dados de alta antes o después del restablecimiento de la función intestinal (complicaciones menores: 34,7% versus 39,5%; complicaciones mayores 3,3% versus 3,4%; P = 0,110). CONCLUSIÓN: Dar de alta antes del restablecimiento de la función intestinal tras cirugía colorrectal electiva parece ser seguro en pacientes debidamente seleccionados.
Published: 2020
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25. Switching from omalizumab to mepolizumab: real-life experience from Southern Italy

Author: Girolamo Pelaia, Nicola Scichilone, Onofrio Resta, Carla Maria Irene Quarato, Giulia Scioscia, Corrado Pelaia, Nunzio Crimi, Giovanna Elisiana Carpagnano, Maria Pia Foschino Barbaro, Maria D'Amato, Cecilia Calabrese, Carpagnano, Ge, Pelaia, C, D'Amato, M, Crimi, N, Scichilone, N, Scioscia, G, Resta, O, Calabrese, C, Pelaia, G, Quarato, Cmi, Foschino Barbaro, Mp., Carpagnano G.E., Pelaia C., D'Amato M., Crimi N., Scichilone N., Scioscia G., Resta O., Calabrese C., Pelaia G., Quarato C.M.I., and Foschino Barbaro M.P.
Subjects: Adult, Male, 0301 basic medicine, Pulmonary and Respiratory Medicine, severe asthma, medicine.medical_specialty, Time Factors, Severe asthma, mepolizumab, omalizumab, severe asthma, switching, Omalizumab, Settore MED/10 - Malattie Dell'Apparato Respiratorio, Antibodies, Monoclonal, Humanized, Severity of Illness Index, 03 medical and health sciences, 0302 clinical medicine, real life, Anti-Allergic Agents, Humans, Medicine, switching, Pharmacology (medical), Anti-Asthmatic Agents, Pulmonary Eosinophilia, Intensive care medicine, Lung, Original Research, Aged, Retrospective Studies, lcsh:RC705-779, Drug Substitution, business.industry, mepolizumab, lcsh:Diseases of the respiratory system, Middle Aged, Asthma, Treatment Outcome, 030104 developmental biology, Italy, 030228 respiratory system, omalizumab, Female, business, Mepolizumab, medicine.drug
Abstract: Background: Current availability of several biologic treatments for severe asthma makes it possible to choose the most appropriate for each patient. Sometimes a good percentage of patients with severe asthma may be eligible for biologics that target either the allergic phenotype or the eosinophilic one, but not all respond to that selected as first choice. The aim of our real-life study was to assess whether, for patients with severe eosinophilic allergic asthma, not previously controlled by the anti-IgE omalizumab, the shift to another biologic targeting interleukin-5, such as mepolizumab, may represent a good therapeutic choice. Methods: A total of 41 consecutive patients with severe, persistent allergic, eosinophilic asthma, uncontrolled despite treatment with omalizumab, were enrolled in seven certified Clinical Respiratory Units of Southern Italy (Catania, Catanzaro, Foggia, Bari, Palermo, and two University Respiratory Units of Naples) and shifted to mepolizumab without a wash-out period. Data at baseline, after at least 12 months of therapy with omalizumab, and after at least 12 months of treatment with mepolizumab were collected. Results: After at least 12 months of therapy with mepolizumab, patients experienced a significant decrease in the number of exacerbations/year (5.8 ± 1.8 versus 0.7 ± 0.9, p Conclusion: Results of our real-life multicentric experience confirms that the shift to mepolizumab could be a good therapeutic strategy in severe eosinophilic allergic asthma not previously controlled by omalizumab. The reviews of this paper are available via the supplemental material section.
Published: 2020

26. Laparoscopic right hemicolectomy: the SICE (Società Italiana di Chirurgia Endoscopica e Nuove Tecnologie) network prospective trial on 1225 cases comparing intra corporeal versus extra corporeal ileo-colic side-to-side anastomosis

Author: Anania G., Agresta F., Artioli E., Rubino S., Resta G., Vettoretto N., Petz W. L., Bergamini C., Arezzo A., Valpiani G., Morotti C., Silecchia G., Adamo V., Agrusa A., Alemanno G., Allaix M. E., Alo A., Altamura A., Ambrosi A., Antoniutti M., Apa D., Arcuri G., Baiocchi G. L., Balani A., Baldazzi G., Basti M., Benvenuto C., Berti S., Boni L., Borghi F., Botteri E., Brachet Contul R., Brescia A., Budassi A., Cafagna L., Calgaro M., Calo P. G., Campagnacci R., Canova G., Canu G. L., Caracino V., Carcoforo P., Carlini M., Casali L., Cassetti D., Cassinotti E., Catarci M., Cesari M., Checcacci P., Ciano P., Clementi M., Cocorullo G., Colombo F., Concone G., Contine A., Coppola M., Coratti A., Corcione F., Corleone P., Covotta L., Cuccurullo D., Cumbo P., D'ambrosio G., De Angelis F., De Luca M., De Manzini N., De Nisco C., De Palma G. D., De Paolis P., Degiuli M., Delogu D., Delrio P., Deserra A., Donini A., Elmore U., Ercolani G., Erdas E., Fabris L., Ferrari G., Feo G., Fidanza F., Foschi D., Galleano R., Garulli G., Gatti F., Gattolin A., Gelati S., Gelmini R., Ghazouani O., Gioffre A., Gobbi S., Grammatico V., Guariniello A., Giannessi S., Guerrieri M., Guerriero L., Gullotta G., Impellizzeri H., Izzo M., Jovine E., Lezoche G., Lirusso C., Lombardi R., Longoni M., Lucchi A., Luzzi A. P., Marini P., Marrosu A. G., Martino A., Mazza R., Mazzoccato S., Medas F., Meloni A., Milone M., Minciotti E., Monari F., Moretto G., Muttillo I. A., Navarra G., Neri S., Oldani A., Olmi S., Opocher E., Osenda E., Ottonello R., Panebianco V., Pavanello M., Pecchini F., Pellegrino L., Pennisi D., Perrotta N., Pertile D., Petri R., Picchetto A., Piccoli M., Pirrera B., Pisani Ceretti A., Pisano M., Podda M., Portolani N., Presenti L., Puzziello A., Razzi S., Rega D., Restini E., Ricci G., Rigamonti M., Rivolta U., Robustelli V., Romairone E., Rosati R., Rosso E., Roviello F., Sala S., Santarelli M., Sarro G., Sartori A., Scabini S., Scognamillo F., Sechi R., Solaini L., Soliani G., Soliani P., Soligo E., Sorrentino M., Spinoglio G., Stratta E., Taddei A., Talamo G., Targa S., Tartaglia N., Testa S., Ubiali P., Valeri A., Vasta F., Verzelli A., Vicentini R., Viola G., Violi V., Zago M., Zampino L., Anania, G., Agresta, F., Artioli, E., Rubino, S., Resta, G., Vettoretto, N., Petz, W. L., Bergamini, C., Arezzo, A., Valpiani, G., Morotti, C., Silecchia, G., Adamo, V., Agrusa, A., Alemanno, G., Allaix, M. E., Alo, A., Altamura, A., Ambrosi, A., Antoniutti, M., Apa, D., Arcuri, G., Baiocchi, G. L., Balani, A., Baldazzi, G., Basti, M., Benvenuto, C., Berti, S., Boni, L., Borghi, F., Botteri, E., Brachet Contul, R., Brescia, A., Budassi, A., Cafagna, L., Calgaro, M., Calo, P. G., Campagnacci, R., Canova, G., Canu, G. L., Caracino, V., Carcoforo, P., Carlini, M., Casali, L., Cassetti, D., Cassinotti, E., Catarci, M., Cesari, M., Checcacci, P., Ciano, P., Clementi, M., Cocorullo, G., Colombo, F., Concone, G., Contine, A., Coppola, M., Coratti, A., Corcione, F., Corleone, P., Covotta, L., Cuccurullo, D., Cumbo, P., D'Ambrosio, G., De Angelis, F., De Luca, M., De Manzini, N., De Nisco, C., De Palma, G. D., De Paolis, P., Degiuli, M., Delogu, D., Delrio, P., Deserra, A., Donini, A., Elmore, U., Ercolani, G., Erdas, E., Fabris, L., Ferrari, G., Feo, G., Fidanza, F., Foschi, D., Galleano, R., Garulli, G., Gatti, F., Gattolin, A., Gelati, S., Gelmini, R., Ghazouani, O., Gioffre, A., Gobbi, S., Grammatico, V., Guariniello, A., Giannessi, S., Guerrieri, M., Guerriero, L., Gullotta, G., Impellizzeri, H., Izzo, M., Jovine, E., Lezoche, G., Lirusso, C., Lombardi, R., Longoni, M., Lucchi, A., Luzzi, A. P., Marini, P., Marrosu, A. G., Martino, A., Mazza, R., Mazzoccato, S., Medas, F., Meloni, A., Milone, M., Minciotti, E., Monari, F., Moretto, G., Muttillo, I. A., Navarra, G., Neri, S., Oldani, A., Olmi, S., Opocher, E., Osenda, E., Ottonello, R., Panebianco, V., Pavanello, M., Pecchini, F., Pellegrino, L., Pennisi, D., Perrotta, N., Pertile, D., Petri, R., Picchetto, A., Piccoli, M., Pirrera, B., Pisani Ceretti, A., Pisano, M., Podda, M., Portolani, N., Presenti, L., Puzziello, A., Razzi, S., Rega, D., Restini, E., Ricci, G., Rigamonti, M., Rivolta, U., Robustelli, V., Romairone, E., Rosati, R., Rosso, E., Roviello, F., Sala, S., Santarelli, M., Sarro, G., Sartori, A., Scabini, S., Scognamillo, F., Sechi, R., Solaini, L., Soliani, G., Soliani, P., Soligo, E., Sorrentino, M., Spinoglio, G., Stratta, E., Taddei, A., Talamo, G., Targa, S., Tartaglia, N., Testa, S., Ubiali, P., Valeri, A., Vasta, F., Verzelli, A., Vicentini, R., Viola, G., Violi, V., Zago, M., Zampino, L., Anania G., Agresta F., Artioli E., Rubino S., Resta G., Vettoretto N., Petz W.L., Bergamini C., Arezzo A., Valpiani G., Morotti C., Silecchia G, and Adamo V, Agrusa A, Alemanno G, Allaix ME, Alò A, Altamura A, Ambrosi A, Antoniutti M, Apa D, Arcuri G, Baiocchi GL, Balani A, Baldazzi G, Basti M, Benvenuto C, Berti S, Boni L, Borghi F, Botteri E, Brachet Contul R, Brescia A, Budassi A, Cafagna L, Calgaro M, Calò PG, Campagnacci R, Canova G, Canu GL, Caracino V, Carcoforo P, Carlini M, Casali L, Cassetti D, Cassinotti E, Catarci M, Cesari M, Checcacci P, Ciano P, Clementi M, Cocorullo G, Colombo F, Concone G, Contine A, Coppola M, Coratti A, Corcione F, Corleone P, Covotta L, Cuccurullo D, Cumbo P, D'Ambrosio G, De Angelis F, De Luca M, De Manzini N, De Nisco C, De Palma GD, De Paolis P, Degiuli M, Delogu D, Delrio P, Deserra A, Donini A, Elmore U, Ercolani G, Erdas E, Fabris L, Ferrari G, Feo C, Fidanza F, Foschi D, Galleano R, Garulli G, Gatti F, Gattolin A, Gelati S, Gelmini R, Ghazouani O, Gioffrè A, Gobbi S, Grammatico V, Guariniello A, Giannessi S, Guerrieri M, Guerriero L, Guerriero G, Impellizzeri H, Izzo M, Jovine E, Lezoche G, Lirusso C, Lombardi R, Longoni M, Lucchi A, Luzzi AP, Marini P, Marrosu AG, Martino A, Mazza R, Mazzoccato S, Medas F, Meloni A, Milone M, Minciotti E, Monari F, Moretto G, Muttillo IA, Navarra G, Neri S, Oldani A, Olmi S, Opocher E, Osenda E, Ottonello R, Panebianco V, Pavanello M, Pecchini F, Pellegrino L, Pennisi D, Perrotta N, Pertile D, Petri R, Picchetto A, Piccoli M, Pirrera B, Pisani Ceretti A, Pisano M, Podda M, Portolani N, Presenti L, Puzziello A, Razzi S, Rega D, Restini E, Ricci G, Rigamonti M, Rivolta U, Robustelli V, Romairone E, Rosati R, Rosso E, Roviello F, Sala S, Santarelli M, Sarro G, Sartori A, Scabini S, Scognamillo F, Sechi R, Solaini L, Soliani G, Soliani P, Soligo E, Sorrentino M, Spinoglio G, Stratta E, Taddei A, Talamo G, Targa S, Tartaglia N, Testa S, Ubiali P, Valeri A, Vasta F, Verzelli A, Vicentini R, Viola G, Violi V, Zago M, Zampino L.
Subjects: Male, medicine.medical_specialty, Anastomosis, Colon, Intracorporeal anastomosis, Outcomes, Laparoscopic colectomy, Article, Intracorporeal anastomosi, Ileo-colic anastomosis, Laparoscopy, Postoperative complications, Right hemicolectomy, Aged, Anastomosis, Surgical, Colectomy, Colonic Neoplasms, Female, Humans, Prospective Studies, Treatment Outcome, Economica, Surgical, medicine, LS7_1, LS7_4, Right hemicolectomy, Ileo-colic anastomosis, Laparoscopy, Postoperative complications, Intracorporeal anastomosis, Outcomes, Outcome, LS7_9, medicine.diagnostic_test, business.industry, General surgery, Right hemicolectomy · Ileo-colic anastomosis · Laparoscopy · Postoperative complications · Intracorporeal anastomosis · Outcomes, Correction, Postoperative complication, Ileo-colic anastomosi, Prospective trial, Surgery, Side to side anastomosis, business, Laparoscopic right hemicolectomy
Abstract: Background While laparoscopic approach for right hemicolectomy (LRH) is considered appropriate for the surgical treatment of both malignant and benign diseases of right colon, there is still debate about how to perform the ileo-colic anastomosis. The ColonDxItalianGroup (CoDIG) was designed as a cohort, observational, prospective, multi-center national study with the aims of evaluating the surgeons’ attitude regarding the intracorporeal (ICA) or extra-corporeal (ECA) anastomotic technique and the related surgical outcomes. Methods One hundred and twenty-five Surgical Units experienced in colorectal and advanced laparoscopic surgery were invited and 85 of them joined the study. Each center was asked not to change its surgical habits. Data about demographic characteristics, surgical technique and postoperative outcomes were collected through the official SICE website database. One thousand two hundred and twenty-five patients were enrolled between March 2018 and September 2018. Results ICA was performed in 70.4% of cases, ECA in 29.6%. Isoperistaltic anastomosis was completed in 85.6%, stapled in 87.9%. Hand-sewn enterotomy closure was adopted in 86%. Postoperative complications were reported in 35.4% for ICA and 50.7% for ECA; no significant difference was found according to patients’ characteristics and technologies used. Median hospital stay was significantly shorter for ICA (7.3 vs. 9 POD). Postoperative pain in patients not prescribed opioids was significantly lower in ICA group. Conclusions In our survey, a side-to-side isoperistaltic stapled ICA with hand-sewn enterotomy closure is the most frequently adopted technique to perform ileo-colic anastomosis after any indications for elective LRH. According to literature, our study confirmed better short-term outcomes for ICA, with reduction of hospital stay and postoperative pain. Trial registration Clinical trial (Identifier: NCT03934151).
Published: 2020

27. Quantitative serological evaluation as a valuable tool in the COVID-19 vaccination campaign

Author: Fabio Ciceri, Chiara Di Resta, Davide Ferrari, Elena De Vecchi, Marco Viganò, Alessandra Mangia, Massimo Locatelli, Maria Sestina Spanò, Lucia Zaffarano, Ferrari, D., Mangia, A., Spano, M. S., Zaffarano, L., Vigano, M., Di Resta, C., Locatelli, M., Ciceri, F., and De Vecchi, E.
Subjects: Adult, Male, medicine.medical_specialty, COVID-19 Vaccines, Siemens SARS-CoV-2 IgG, Health Personnel, Clinical Biochemistry, Population, Antibodies, Viral, Serology, Young Adult, Immune system, Immunity, Internal medicine, medicine, Humans, Young adult, education, Aged, Aged, 80 and over, Immunoassay, education.field_of_study, biology, business.industry, Immunization Programs, SARS-CoV-2, Biochemistry (medical), COVID-19, General Medicine, Middle Aged, Immunity, Humoral, Clinical trial, Vaccination, serological test, mRNA vaccine, Roche Anti-SARS-CoV-2-S, biology.protein, Female, Antibody, business
Abstract: Objectives After exceptional research efforts, several vaccines were developed against SARS-CoV-2 which sustains the pandemic COVID-19. The Comirnaty vaccine showed high efficacy in clinical trials and was the first to be approved for its distribution to the general population. We evaluated the immune response induced by the first vaccine dose in different sex/age groups and subjects with or without naturally present anti-SARS-CoV-2 antibodies. Methods As part of an Italian multicenter project (Covidiagnostix), serum samples from 4,290 health-professionals were serologically tested the day of the first vaccination dose, and 21 days later, using two different instrumentations (Siemens-Healthineers and Roche). Results In total, 97% of samples showed the presence of specific antibodies 21 days after the vaccination dose; the percentage of non-responders increased with age in both genders. Remarkably, naturally seropositive individuals showed antibody persistence up to 11 months and an exceptionally higher vaccination response compared to subjects never infected by SARS-CoV-2. Conclusions This study highlighted the importance of the serological test i) to identify naturally SARS-CoV-2 seropositive individuals and ii) to evaluate the antibody level elicited by the first vaccination dose. Both tests, highlighted differences in the immune response, when subjects were stratified by sex and age, and between naturally seropositive and seronegative subjects. The data obtained show how serological tests could play a crucial role in the triage of the population subjected to the vaccination campaign for COVID-19. The definition of suitable instrumentation-specific thresholds is needed to correctly follow eventually acquired post-vaccination immunity in the general population.
Published: 2021

28. Politicization of COVID-19 health-protective behaviors in the United States: Longitudinal and cross-national evidence

Author: Stroebe, Wolfgang, vanDellen, Michelle R., Abakoumkin, Georgios, Lemay, Edward P., Schiavone, William M., Agostini, Maximilian, Bélanger, Jocelyn J., Gützkow, Ben, Kreienkamp, Jannis, Reitsema, Anne Margit, Abdul Khaiyom, Jamilah Hanum, Ahmedi, Vjolica, Akkas, Handan, Almenara, Carlos A., Atta, Mohsin, Bagci, Sabahat Cigdem, Basel, Sima, Kida, Edona Berisha, Bernardo, Allan B.I., Buttrick, Nicholas R., Chobthamkit, Phatthanakit, Choi, Hoon Seok, Cristea, Mioara, Csaba, Sára, Damnjanović, Kaja, Danyliuk, Ivan, Dash, Arobindu, Di Santo, Daniela, Douglas, Karen M., Enea, Violeta, Faller, Daiane Gracieli, Fitzsimons, Gavan, Gheorghiu, Alexandra, Gómez, Ángel, Hamaidia, Ali, Han, Qing, Helmy, Mai, Hudiyana, Joevarian, Jeronimus, Bertus F., Jiang, Ding Yu, Jovanović, Veljko, Kamenov, Željka, Kende, Anna, Keng, Shian Ling, Kieu, Tra Thi Thanh, Koc, Yasin, Kovyazina, Kamila, Kozytska, Inna, Krause, Joshua, Kruglanksi, Arie W., Kurapov, Anton, Kutlaca, Maja, Lantos, Nóra Anna, Jaya Lemsmana, Cokorda Bagus, Louis, Winnifred R., Lueders, Adrian, Malik, Najma Iqbal, Martinez, Anton, McCabe, Kira O., Mehulić, Jasmina, Milla, Mirra Noor, Mohammed, Idris, Molinario, Erica, Moyano, Manuel, Silvana Mula, Hayat Muhammad, Muluk, Hamdi, Myroniuk, Solomiia, Najafi, Reza, Nisa, Claudia F., Nyúl, Boglárka, O’Keefe, Paul A., Olivas Osuna, Jose Javier, Osin, Evgeny N., Park, Joonha, Pica, Gennaro, Pierro, Antonio, Rees, Jonas, Resta, Elena, Rullo, Marika, Ryan, Michelle K., Samekin, Adil, Santtila, Pekka, Sasin, Edyta, Schumpe, Birga M., Selim, Heyla A., Stanton, Michael Vicente, Sultana, Samiah, Sutton, Robbie M., Tseliou, Eleftheria, Utsugi, Akira, van Breen, Jolien Anne, van Lissa, Caspar J., van Veen, Kees, Vázquez, Alexandra, Wollast, Robin, Yeung, Victoria Wai Lan, Zand, Somayeh, Žeželj, Iris Lav, Zheng, Bang, Zick, Andreas, Zúñiga, Claudia, Pontus Leander, N., Afd Sociale-,gezondheids- en organ.psych, Leerstoel Heijden, Methodology and statistics for the behavioural and social sciences, Sociale Psychologie (Psychologie, FMG), Afd Sociale-,gezondheids- en organ.psych, Leerstoel Heijden, Methodology and statistics for the behavioural and social sciences, Social Psychology, Developmental Psychology, Research programme OB, Research programme GEM, and Organizational Psychology
Subjects: Male, Viral Diseases, Epidemiology, Cross-sectional study, Health Behavior, Social Sciences, 050109 social psychology, Political Aspects of Health, Surveys, Social Distancing, Biology and political orientation, Governments, Medical Conditions, Pandemic, Medicine and Health Sciences, Public and Occupational Health, Longitudinal Studies, Pandemics/prevention & control, COVID-19/epidemiology, Adolescent, adult, aged, cross-sectional studies, humans, longitudinal studies, male, middle aged, pandemics, COVID-19, health behavior, motivation, politics, SARS-CoV-2, Multidisciplinary, 05 social sciences, Politics, Middle Aged, 3. Good health, Infectious Diseases, Research Design, Medicine, Health behavior, Behavioral and Social Aspects of Health, Psychology, Social psychology, Research Article, Political Parties, Cross national, Adult, Infectious Disease Control, Coronavirus disease 2019 (COVID-19), Political Science, Science, Research and Analysis Methods, 050105 experimental psychology, Humans, 0501 psychology and cognitive sciences, Pandemics, Aged, Motivation, Survey Research, Correction, Covid 19, Risk perception, Cross-Sectional Studies, Medical Risk Factors, Initial phase
Abstract: During the initial phase of the COVID-19 pandemic, U.S. conservative politicians and the media downplayed the risk of both contracting COVID-19 and the effectiveness of recommended health behaviors. Health behavior theories suggest perceived vulnerability to a health threat and perceived effectiveness of recommended health-protective behaviors determine motivation to follow recommendations. Accordingly, we predicted that—as a result of politicization of the pandemic—politically conservative Americans would be less likely to enact recommended health-protective behaviors. In two longitudinal studies of U.S. residents, political conservatism was inversely associated with perceived health risk and adoption of health-protective behaviors over time. The effects of political orientation on health-protective behaviors were mediated by perceived risk of infection, perceived severity of infection, and perceived effectiveness of the health-protective behaviors. In a global cross-national analysis, effects were stronger in the U.S. (N = 10,923) than in an international sample (total N = 51,986), highlighting the increased and overt politicization of health behaviors in the U.S.
Published: 2021

29. Neurofibromatosis type 1 and melanoma of the iris arising from a dysplastic nevus: A rare yet casual association?

Author: Alessandro Stella, Nicoletta Resta, Veronica Falcone, Giovanni Alessio, Domenico Piscitelli, Silvana Guerriero, Valeria Albano, and Patrizia Lastella
Subjects: Male, 0301 basic medicine, medicine.medical_specialty, Neurofibromatosis 1, Skin Neoplasms, Iris, 03 medical and health sciences, 0302 clinical medicine, Dysplastic nevus syndrome, medicine, Humans, Neurofibromatosis, Iris (anatomy), Melanoma, biology, business.industry, Iris Lesion, Iris melanoma, General Medicine, Middle Aged, medicine.disease, Dermatology, Neurofibromin 1, eye diseases, Ophthalmology, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Dysplastic nevus, biology.protein, business, Dysplastic Nevus Syndrome
Abstract: Purpose: We investigated the molecular causes of an unusual pigmented and ulcerated iris lesion detected in a patient diagnosed with neurofibromatosis type 1 (NF1). Case Report: A 52-year-old man was referred to our clinic with a non-traumatic ulcer in his left eye. Hyphema reabsorption disclosed a pigmented iris mass, thus ultrasound biomicroscopy and anterior segment fluorescein angiography were performed to investigate for the presence of a malignant lesion. Upon angiography, the lesion appeared highly vascularized but prevented posterior iris examination. Therefore, a gonioscopy was executed revealing extension of the lesion into the peripheral iris. Histopathology of the excisional iris biopsy revealed iris melanoma over a dysplastic nevus. NF1 is an autosomal dominant disorder characterized by pigmented cutaneous lesions, multiple skin tumors, and spinal and cranial nerve tumors. Uveal melanoma is the most common primary intraocular malignancy in adults. Up to 92% of cutaneous melanomas occur in patients with dysplastic nevus syndrome. Skin melanomas have been found in 0.1%–5.4% of NF1 patients. In literature, only 18 reports of uveal melanoma have been documented in association with NF1, including three cases of iris melanoma. Results: NF1 gene testing identified a causative mutation in the germline but no loss of the wild-type allele in the iris melanoma. Conclusions: Occurrence of both diseases in one patient is extremely rare, but the common origin of Schwann cells and melanoblasts suggests a non-casual association. Therefore, we propose that NF1 patients should be screened for nevi, both cutaneous and uveal, for better patients’ management.
Published: 2020
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30. Calcium priming of the central venous catheter prevents a drop in ionized calcium concentration during Regional Citrate Anticoagulation

Author: Luca Di Girolamo, Mattia Busana, Marco Dei Poli, Federica Ferrari, Marco Resta, Andrea C Lusardi, Giacomo Trevisan, and Giulia Roveri
Subjects: Male, Dead space, medicine.medical_treatment, Biomedical Engineering, Biophysics, chemistry.chemical_element, Pilot Projects, Bioengineering, 030204 cardiovascular system & hematology, Calcium, Citric Acid, Biomaterials, Calcium Chloride, 03 medical and health sciences, 0302 clinical medicine, Dialysis Solutions, medicine, Central Venous Catheters, Humans, Renal replacement therapy, Blood Coagulation, Saline, Aged, Calcium metabolism, Hypocalcemia, Anticoagulants, General Medicine, Blood flow, Middle Aged, 3. Good health, Catheter, 030228 respiratory system, chemistry, Anesthesia, Female, Hemofiltration, Central venous catheter
Abstract: During citrate-based Continuous Renal Replacement Therapy (CRRT), an infusion of calcium is necessary to replace the calcium lost in the effluent. The replacement takes place through a central venous catheter (CVC) that is primed with saline solution. Thus, we hypothesized a potential systemic anticoagulation caused by the unchelated citrate reaching the patient at the start of CRRT because of 0.42 ml of line dead space. In this pilot study, two subpopulations of 7 patients who underwent Continuous Veno-Venous Hemodiafiltration (150 ml/min of blood flow, 1500 ml/h dialysate flow, 1500 ml/h of citrate predilution) were studied. One had the CVC primed with saline, the second with calcium chloride 10%. Calcium replacement rate was 6.3 ± 0.2 ml/h. Ionized calcium concentration was studied over time in the two groups and in the group with saline priming we detected a transient period of hypocalcemia (ionized calcium concentration [iCa] < 1.00 mmol/l for the first 2 hours). In the subpopulation with the calcium priming, this was not present. No significant effect on filter life emerged. Priming of the catheter with calcium seems effective in avoiding a potential issue regarding citrate accumulation at the start of CRRT. More studies are needed to assess the clinical significance of this finding.
Published: 2019
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31. Gastric polyposis and desmoid tumours as a new familial adenomatous polyposis clinical variant associated with APC mutation at the extreme 3′-end

Author: Alessandro Stella, Martina Lepore Signorile, Ivan Lolli, Valentina Grossi, Candida Fasano, Claudio Lotesoriere, Filomena Cariola, Nicoletta Resta, Vittoria Disciglio, Cristiano Simone, Giovanna Forte, and Paola Sanese
Subjects: Adult, Male, 0301 basic medicine, congenital, hereditary, and neonatal diseases and abnormalities, Adenomatous Polyposis Coli Protein, medicine.disease_cause, Germline, Familial adenomatous polyposis, 03 medical and health sciences, 0302 clinical medicine, Germline mutation, Stomach Neoplasms, Genetics, medicine, Humans, Genetic Predisposition to Disease, Gastric Polyposis, Gene, Germ-Line Mutation, Genetics (clinical), Mutation, gastric polyposis, business.industry, Gastric Polyposis and Desmoid FAP, Genotype-Phenotype Correlations, FAP, Middle Aged, medicine.disease, Phenotype, APC C-terminus, digestive system diseases, Pedigree, APC, Fibromatosis, Aggressive, 030104 developmental biology, Adenomatous Polyposis Coli, 030220 oncology & carcinogenesis, Familial Adenomatous Polyposis, Cancer research, Female, Desmoid tumours, business
Abstract: Germline mutations of the APC gene, which encodes a multidomain protein of 2843 amino acid residues, cause familial adenomatous polyposis (FAP). Three FAP clinical variants are correlated with the location of APC mutations: (1) classic FAP with profuse polyposis (>1000 adenomas), associated with mutations from codon 1250 to 1424; (2) attenuated FAP (APC extremities (before codon 157 and after codon 1595); (3) classic FAP with intermediate colonic polyposis (100–1000 adenomas), associated with mutations located in the remaining part of APC. In an effort to decipher the clinical phenotype associated with APC C-terminal germline truncating mutations in patients with FAP, after screening APC mutations in one family whose members (n=4) developed gastric polyposis, colon oligo-polyposis and desmoid tumours, we performed a literature meta-analysis of clinically characterised patients (n=97) harbouring truncating mutations in APC C-terminus. The APC distal mutations identified in this study cluster with a phenotype characterised by colon oligo-polyposis, diffuse gastric polyposis and desmoid tumours. In conclusion, we describe a novel FAP clinical variant, which we propose to refer to as Gastric Polyposis and Desmoid FAP, that may require tailored management.
Published: 2019
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32. Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension

Author: Kenneth G. Vigil, Simin Yan, Benjimen R. Walker, Joshua R. Sheak, Lindsay M. Herbert, Neil D. Detweiler, Nikki L. Jernigan, Selina Garcia, and Thomas C. Resta
Subjects: Male, 0301 basic medicine, medicine.medical_specialty, Acid-Sensing Ion Channel 2, Physiology, Hypertension, Pulmonary, Myocytes, Smooth Muscle, Pulmonary Artery, Vascular Remodeling, Muscle, Smooth, Vascular, Mice, 03 medical and health sciences, 0302 clinical medicine, Acute hypoxia, Physiology (medical), Internal medicine, Animals, Medicine, Plethysmograph, Calcium Signaling, Hypoxia, Lung, Mice, Knockout, business.industry, medicine.disease, Pulmonary hypertension, Acid Sensing Ion Channels, Mice, Inbred C57BL, 030104 developmental biology, Blood pressure, medicine.anatomical_structure, Vasoconstriction, Cardiology, Vascular resistance, Calcium, Female, Vascular Resistance, business, 030217 neurology & neurosurgery, Research Article
Abstract: Acid-sensing ion channels (ASICs) are voltage-insensitive cation channels that contribute to cellular excitability. We previously reported that ASIC1 in pulmonary artery smooth muscle cells (PASMC) contribute to pulmonary vasoreactivity and vascular remodeling during the development of chronic hypoxia (CH)-induced pulmonary hypertension. However, the roles of ASIC2 and ASIC3 in regulation of pulmonary vasoreactivity and the development of CH-induced pulmonary hypertension are unknown. We tested the hypothesis that ASIC2 and ASIC3 contribute to increased pulmonary vasoreactivity and development of CH-induced pulmonary hypertension using ASIC2- and ASIC3-knockout (−/−) mice. In contrast to this hypothesis, we found that ASIC2−/−mice exhibit enhanced CH-induced pulmonary hypertension compared with WT and ASIC3−/−mice. This response was not associated with a change in ventilatory sensitivity or systemic cardiovascular function but was instead associated with direct changes in pulmonary vascular reactivity and pulmonary arterial morphology in ASIC2−/−mice. This increase in reactivity correlated with enhanced pulmonary arterial basal tone, elevated basal PASMC [Ca2+] and store-operated calcium entry (SOCE) in PASMC from ASIC2−/−mice. This increase in PASMC [Ca2+] and vasoreactivity was dependent on ASIC1-mediated Ca2+influx but was not contingent upon an increase in ASIC1 mRNA or protein expression in PASMC from ASIC2−/−mice. Together, the results from this study demonstrate an important role for ASIC2 to regulate pulmonary vascular reactivity and for ASIC2 to modulate the development of CH-induced pulmonary hypertension. These data further suggest that loss of ASIC2 enhances the contribution of ASIC1 to overall pulmonary vascular reactivity.NEW & NOTEWORTHY This study demonstrates that loss of ASIC2 leads to increased baseline pulmonary vascular resistance, enhanced responses to a variety of vasoconstrictor stimuli, and greater development of hypoxic pulmonary hypertension. Furthermore, these results suggest that loss of ASIC2 enhances the contribution of ASIC1 to pulmonary vascular reactivity.
Published: 2019
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33. Relationships between Obstructive Sleep Apnea Syndrome and cardiovascular risk in a naïve population of southern Italy

Author: Onofrio Resta, Silvano Dragonieri, Pierluigi Carratù, Agostino Di Ciaula, Piero Portincasa, Marco Matteo Ciccone, and Teresa Ranieri
Subjects: Male, medicine.medical_specialty, Polysomnography, Population, Overweight, Pulmonary function testing, Body Mass Index, Risk Factors, Internal medicine, medicine, Humans, Endothelial dysfunction, education, education.field_of_study, Sleep Apnea, Obstructive, business.industry, General Medicine, Anthropometry, Middle Aged, medicine.disease, Obesity, Obstructive sleep apnea, Cardiovascular Diseases, Heart Disease Risk Factors, Ambulatory, Female, medicine.symptom, business
Abstract: BACKGROUND Obstructive sleep apnea (OSA) is a worldwide increasing syndrome, which, by promoting endothelial dysfunction, contributes to extend the cardiovascular risk. We evaluated the cardiovascular risk in a group of OSA patients. METHODS A total of 185 OSA subjects (19 normal weight, 57 overweight, 109 obese), who entered the Ambulatory of Sleep Disorders of the Institute of Respiratory Diseases of the University of Bari, during 1 year, were enrolled in the study. We assessed anthropometric features, polysomnographic findings, cardiovascular risk factors, smoking habit, Pulmonary Function Test, Arterial Blood Gas Analysis, Epworth Questionnaire, and Charlson Co-morbidities Index (CCI). Subjects were divided into three groups, according to their BMI: individuals with BMI ≥30 kg/m2 (Group 1 n = 109, mean age 61 ± 1; 74.3% men), individuals with BMI ranging from 25.0 to 29.9 kg/m2 defined as overweight subjects (Group 2 n = 57, mean age 58.8 ± 1.4; 77% men), and subjects with a BMI ranging from 18.5 to 24.9 kg/m2 defined as normal weight subjects (Group 3 n = 19, mean age 54.2 ± 2.3; 64,2% men). RESULTS In the whole population, the percentage cardiovascular risk was weakly related with BMI (r = 0.33; P
Published: 2021

34. Pro-inflammatory cytokines as emerging molecular determinants in cardiolaminopathies

Author: Maria Svelto, Monica Carmosino, Stefano Favale, Giuseppe Procino, Serena Milano, Andrea Gerbino, Piero Guida, Martino Pepe, Cinzia Forleo, Mara Piccolo, Nicoletta Resta, and Francesca Piccapane
Subjects: Adult, Male, Chemokine, Heart Diseases, Cardiolipins, Inflammation, medicine.disease_cause, Proinflammatory cytokine, Cell Line, LMNA, medicine, Humans, Interleukin 6, Mutation, biology, business.industry, cardiolaminopathies, Interleukin, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, Original Articles, Middle Aged, Phenotype, Recombinant Proteins, cytokines, HEK293 Cells, Immunology, biology.protein, Molecular Medicine, Female, Original Article, medicine.symptom, business
Abstract: Mutations in Lamin A/C gene (lmna) cause a wide spectrum of cardiolaminopathies strictly associated with significant deterioration of the electrical and contractile function of the heart. Despite the continuous flow of biomedical evidence, linking cardiac inflammation to heart remodelling in patients harbouring lmna mutations is puzzling. Therefore, we profiled 30 serum cytokines/chemokines in patients belonging to four different families carrying pathogenic lmna mutations segregating with cardiac phenotypes at different stages of severity (n = 19) and in healthy subjects (n = 11). Regardless lmna mutation subtype, high levels of circulating granulocyte colony‐stimulating factor (G‐CSF) and interleukin 6 (IL‐6) were found in all affected patients’ sera. In addition, elevated levels of Interleukins (IL) IL‐1Ra, IL‐1β IL‐4, IL‐5 and IL‐8 and the granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) were measured in a large subset of patients associated with more aggressive clinical manifestations. Finally, the expression of the pro‐inflammatory 70 kDa heat shock protein (Hsp70) was significantly increased in serum exosomes of patients harbouring the lmna mutation associated with the more severe phenotype. Overall, the identification of patient subsets with overactive or dysregulated myocardial inflammatory responses could represent an innovative diagnostic, prognostic and therapeutic tool against Lamin A/C cardiomyopathies.
Published: 2021

35. Integrating video tracking and GPS to quantify accelerations and decelerations in elite soccer

Author: Tomás García-Calvo, Juan José Pulido-González, Eduard Pons, Hugo Blanco, Ricardo Resta, Roberto López-Del Campo, Francesc Cos, and Jesús Díaz-García
Subjects: Adult, Male, Intraclass correlation, Science, Video Recording, Magnitude (mathematics), Athletic Performance, Article, Degree (temperature), Running, Acceleration, Young Adult, Consistency (statistics), Statistics, Soccer, Humans, Mathematics, Multidisciplinary, Biological techniques, Gold standard (test), Computational biology and bioinformatics, Assisted GPS, Video tracking, Geographic Information Systems, Medicine
Abstract: The aim of this study was to analyze the degree of agreement comparing number and distance covered in different acceleration and deceleration sections registered by a video tracking system (MEDIACOACH) and a GPS device (WIMU PRO) during official competition. Data from a Spanish professional club were registered over the course of a season. First, the descriptive statistics presented more bursts of accelerations and decelerations in WIMU PRO than in MEDIACOACH, whereas the distances covered recorded by both systems were similar. Second, negative relationships were found (i.e., negative bias) comparing WIMU PRO to MEDIACOACH in the number of accelerations and decelerations between 0/1 m/s2 and ½ m/s2 (p p 2 and in accelerations and decelerations registered between 2/3 m/s2 and more than 3 m/s2. Moreover, the differences in means (i.e., standardized mean bias) across the two devices were trivial (> 0.19) and small (0.2–0.59) for most variables. The standardized typical errors in the estimate (TEE) were moderate (0.3–0.59) and small to moderate (0.1–0.29 to 0.3–0.59), respectively. Also, the Intra class Correlation Coefficients (ICCs) for agreement and consistency between systems showed good and excellent values (> 0.90). The magnitude of change in means (%) between systems, defined as the percentage change between the numbers or values, was below 14% and 7% for number and distances covered, respectively. All scores in the smallest worthwhile change were lower than 9% and in the coefficients of variation were lower than 95% and 15%, respectively. Thus, both systems demonstrated an acceptable degree of agreement and could be useful in analyzing players’ acceleration demands in professional soccer. However, caution is required when interpreting the results and a comparison with a gold standard is required in order to validate both systems.
Published: 2021

36. HMGB1-TIM3-HO1: A New Pathway of Inflammation in Skin of SARS-CoV-2 Patients? A Retrospective Pilot Study

Author: Caterina Foti, Aurora Demarco, Paola Tarantino, Pragnell Maria Victoria Candance, Gerardo Cazzato, Lucia Lospalluti, Giuseppe Ingravallo, Teresa Lettini, Sara Sablone, Leonardo Resta, Anna Colagrande, Paolo Romita, Vincenza Sara Scarcella, Antonietta Cimmino, Sebastiano Cicco, and Gerolamo Cicco
Subjects: Adult, Male, skin, T-Lymphocytes, TIM-3, Population, HO-1, Inflammation, Biochemistry, Microbiology, Skin Diseases, Article, Immune system, Immunopathology, medicine, Humans, HMGB1 Protein, education, Molecular Biology, Hepatitis A Virus Cellular Receptor 2, Aged, HMGB1, education.field_of_study, medicine.diagnostic_test, business.industry, SARS-CoV-2, COVID-19, Middle Aged, QR1-502, Heme oxygenase, Immunology, Skin biopsy, Heme Oxygenase (Decyclizing), Immunohistochemistry, Female, medicine.symptom, business, CD8
Abstract: The SARS-CoV-2 pandemic has completely disrupted the health systems of the entire planet. From the earliest months, it became increasingly clear that in addition to affecting the upper airways and lungs, there were other organs that could be affected. Among these, the skin became a real “sentinel signal” to be able to even suspect COVID-19. Background: this study deals with a little-explored issue for now: the study of skin immunopathology in SARS-CoV-2 positive subjects ascertained using the most reliable methods available. Methods: we used skin biopsy samples from SARS-CoV-2 positive and negative patients, studying morphology (Hematoxylin-Eosin), T lymphocyte population (CD4 and CD8), three markers such as HMGB-1, TIM-3 and HO-1 by immunohistochemistry. Results: although the presence of the CD4 and CD8 T population did not differ statistically significantly, we found greater activation and release of HMGB-1 in skin samples from SARS-CoV-2 positive patients, greater immunolabeling for TIM-3 at the level of CD4 and CD8 and a reduced expression of Heme oxygenase 1. Conclusions: these results support the possibility that there is immune deregulation in SARS-CoV-2 positive patients who develop skin manifestations of various kinds.
Published: 2021
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37. SARS-CoV-2 and Skin: The Pathologist’s Point of View

Author: Anna Colagrande, Sara Sablone, Giulia Mazzia, Rossella Elia, Eleonora Nacchiero, Roberta Rossi, Giuseppe Ingravallo, Teresa Lettini, Gerardo Cazzato, Leonardo Resta, Antonietta Cimmino, Giuseppe Giudice, Andrea Marzullo, Eugenio Maiorano, Nadia Santarella, and Michele Maruccia
Subjects: Adult, Male, Pathology, medicine.medical_specialty, skin, Erythema, Coronavirus disease 2019 (COVID-19), Adolescent, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Disease, Biochemistry, Skin Diseases, Microbiology, Article, 030207 dermatology & venereal diseases, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Pandemic, Medicine, Humans, Child, Molecular Biology, Skin manifestations, Lung, business.industry, SARS-CoV-2, COVID-19, Middle Aged, QR1-502, eruption, medicine.anatomical_structure, Infectious disease (medical specialty), 030220 oncology & carcinogenesis, Female, medicine.symptom, business
Abstract: The SARS-CoV-2 pandemic has dramatically changed our lives and habits. In just a few months, the most advanced and efficient health systems in the world have been overwhelmed by an infectious disease that has caused 3.26 million deaths and more than 156 million cases worldwide. Although the lung is the most frequently affected organ, the skin has also resulted in being a target body district, so much so as to suggest it may be a real “sentinel” of COVID-19 disease. Here we present 17 cases of skin manifestations studied and analyzed in recent months in our Department, immunohistochemical investigations were carried out on samples for the S1 spike-protein of SARS-CoV-2, as well as electron microscopy investigations showing evidence of virions within the constituent cells of the eccrine sweat glands and the endothelium of small blood vessels. Finally, we conduct a brief review of the COVID-related skin manifestations, confirmed by immunohistochemistry and/or electron microscopy, described in the literature.
Published: 2021

38. Safety and efficacy of non-steroidal anti-inflammatory drugs to reduce ileus after colorectal surgery

Author: Chapman, Sj, Clerc, D, Blanco‐colino, R, Otto, A, Nepogodiev, D, Pagano, G, Schaeff, V, Soares, A, Zaffaroni, G, Žebrák, R, Hodson, J, Glasbey, Jc, Pata, P, Pellino, G, Sgrò, A, Elst, T, Van Straten, S, Knowles, Ch, Borakati, A, Bath, Mf, Yasin, Ih, Mclean, K, Arthur, T, Kovacevic, M, Delibegovic, S, Karamanliev, M, Swamad, M, Paramasivam, R, Martensen, A, Larsen, Hm, Rädeker, L, Frey, Pe, Kechagias, A, Venara, A, Duchalais, E, Ioannidis, A, Pata, F, Pasquali, S, Simioni, A, Farina, V, Podda, M, Lorenzon, L, Jakubauskas, M, Elst, Tr, Chu, M, Fagan, Pvb, Wells, Ci, Alagoa João, A, Juloski, J, Ciubotaru, C, Popescu, S, Yanishev, A, Lee, S, Ozkan, Bb, Yagız Sen, A, Aktas, Mk, Baki, Be, Yüksek, B, Kamarajah, S, Khaw, Ra, Mills, E, Goodson, R, Thakral, N, Ablett, Ad, Adra, M, Kwek, I, Khan, Sm, Quinn, P, Manley, Lr, Badran, A, Ramjeeawon, A, Campbell, A, Tan, Hl, Rye, Ds, Rajaraman, N, Norman, Jg, Vutipongsatorn, K, Solomou, G, Akhbari, M, Ali, A, Murray, V, Baker, Dm, Brandao, Bd, Stainer, B, Thavayogan, R, Jones, D, Onafowokan, Oo, Gharooni, A, Dabab, N, Carlton‐carew, S, Kungwengwe, G, Gabriel, M, Sewart, E, Shortland, Tc, Lawday, S, Pockney, P, Dawson, A, Brumfitt, Cd, Stewart, P, Ng, B, Luong, Jk, Ivanov, V, Borisova, A, Neykov, V, Kunčarová, K, Kološová, B, Antonova, T, Farkašová, M, Harbjerg, Jl, Brandsborg, S, Brinck, S, Kjaer, Md, Mark‐christensen, A, Unbehaun, Kp, Dalsgaard, P, Lycke, Kd, Lenaoures, P, Brigand, C, Dumange, E, Gout, M, Moehwald, C, Prem, M, Alhalabi, O, Sliwinski, S, Krupp, J, Gablenz, E, Schmitzer, L, Kopp, A, Steinle, J, Gsenger, J, Pohl, Lj, Riccardi, M, Christodoulou, Im, Konstantinidis, M, Machairas, N, Zoikas, A, Balalis, D, Manatakis, Dk, Aguilera, Ml, Marano, L, Fleres, F, Lovisetto, F, Sasia, D, Segalini, E, Pata, G, Lucchi, A, Sagnotta, A, Campagnaro, T, Petrelli, F, Gallo, G, Papandrea, M, Testa, V, Sinibaldi, G, Di Candido, F, Colombo, F, Perrone, G, Aresu, S, Biancafarina, A, Canonico, G, Pagnanelli, M, Curletti, G, Bini, R, Manzoni Garberini, A, Impellizzeri, H, Cillara, N, Tutino, R, Picciariello, A, Coletta, D, Savino, G, Ferrara, F, Tamini, N, Talamo, G, Parini, D, Giamundo, P, lo Conte, A, Ripetti, V, Pesce, A, Menduni, N, Giudicissi, R, Goldin, E, Rega, D, Belli, A, Andriola, V, Gordini, L, Foppa, C, Piccolo, G, Birindelli, A, Ferrari, C, Ballarini, Z, Tirelli, F, Milone, M, De Rosa, M, Pipitone Federico, Ns, Molteni, B, Tilocca, Pl, Sancini, G, Piozzi, Gn, Lauretta, A, Poillucci, G, Mulas, S, Simcikas, D, Portelli, L, Wijnbergen, Jwm, Dinger, Tl, Doesschate, Sfh, Dalen, Ashm, Bos, Dd, Hansmann, M, Medina Feliz, J, Kuiper, Sz, Abdulrahman, Z, Pruijssers, Sr, Farik, S, Elliott, Bm, Geneta, Vp, Wilton, S, Kandelaki, H, Peng, Sl, Campbell, S, Lim, Yk, Yassaie, Ss, Murray, M, Haran, C, Tan, J, Castro, J, Laranjeira, A, Catarino, S, Neves‐marques, C, Correia, Jg, Vieira, Bn, Quintela, Ac, Serra, Ml, Maciel, J, Cunha, M, Aparício, Dj, Neves, J, Azevedo, J, Romano, M, Eiró, F, Romano, J, Monteiro, C, Claro, M, Almeida, Mr, Peyroteo, M, Machado, Nd, Capote, H, Ferreira, M, Sousa, X, Devesa, H, Cavadas, D, Guerreiro, I, Costa, M, Salman, M, English, C, Mohammed, N, Litvin, A, Ćuk, Vv, Mészárosová, K, Jaich, R, De Lima, H, Brooks, S, Marx, M, Nshalati Salvation, M, Blaser, B, Piazza, G, Gagliardi, B, Serin, H, Yurdaor, Ss, Arslan, E, Kopac, O, Uyanik, A, Ozmen, Bb, Tiftik, E, Aksoy, B, Yalcinkaya, A, Ozoglu, F, Kocer, Md, Bilicen, G, Cinar, En, Uslu, Ö, Kaya, Y, Wong, J, Farhan‐alanie, Mmh, Suresh, G, Asif, A, Finch, Bj, Bhahirathan, Y, Herron, J, Yi Tew, Z, Obukofe, R, Russell, C, Suchett‐kay, I, Netke, T, Williams, L, Kisiel, A, Liu, Fy, Claireaux, H, James, P, Mondal, A, Kalderon, R, Nadama, Hh, Al‐saraff, Z, Tam, Jph, Powell‐chandler, A, Wood, F, Gorgievska, Rebecca, Ragavoodoo, A, Thakrar, C, Rojoa, D, Palmer, C, Davidson, K, Giacci, L, Hale, J, Gan, Fw, Makin‐taylor, R, Hey, Cy, Toh, C, Findlay, Jm, Griffiths, N, Ganesananthan, S, Jasionowska, S, Poustie, M, Wong, C, Turner, T, Pyc, W, Sloper, W, Warner, C, Coey, J, Mason, D, Sait, S, Kowal, M, Owen, M, Saiyed, A, Ashworth, I, Akbari, K, Curran, M, Martin, P, Parker, D, Kwok, K, Lye, C, Ghaly, M, Sammour, T, Lewis, D, Mundasad, R, Wilkes, A, Ctercteko, G, Maslyankov, S, Dimov, R, Iliev, S, Dimitrov, D, Marek, F, Örhalmi, J, Skalický, P, Skalický, T, Chrz, K, Christensen, P, Worsøe, J, Kristensen, Es, Emmertsen, Kj, Loeve, Us, Mihaljevic, Al, Herrle, F, Konstantinidis, Km, Korkolis, D, Karanikas, I, Vincenti, L, Anania, G, Borghi, F, Agresta, F, Maretto, I, Parisi, A, Bucci, L, De Palma, G, Guglielmi, A, Cucinotta, E, La Torre, F, Cianchi, F, Guerrieri, M, Trompetto, M, Persiani, R, Micheletto, G, Cantafio, S, Ronconi, M, Bisagni, Pag, De Prizio, M, Franceschi, A, Galleano, R, Cavallini, M, Brescia, A, D'Ambra, L, Benevento, A, Niolu, P, Calgaro, M, Colangelo, E, Grottola, T, Altomare, Df, Puleo, S, Salamone, G, Pietrabissa, A, Poggioli, G, Erdas, E, Ottonello, R, Tonini, V, Selvaggi, F, Sammarco, G, Ceccarelli, G., 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Staden N., McDuling C.R., Claassen L., Kruger C., Weyers J., Lecler J., Mignon G., Vanoni A., Henkens A., Jarrar G., Odovic M., Willemin M., Milliet O., Hahnloser D., Demartines N., Teixeira H., Pittet O., Haller M.L., Huot A., Kefleyesus A., Bugmann A., Piotet L.M., Meijers L., Kurtoglu G.K., Sel E.K., Isler V.C., Yilmaz B.S., Adiyaman C., Kara M., Demirkaya H.C., Korkmaz H.K., Pektas A.M., Guner B., Islek E.C., Tombul H.B., Boztug C.Y., Tinaz A., Yilmaz M., Karaman Y., Akin F.A., Dansuk S., Bascavus M., Kaya M., Kutlu B., Seker M.C., Erten V., Memis U., Kocak Z.B., Karapinar Y.E., Fakirullahoglu M., Koseoglu E., Yeni M., Aykun N., Agca M.H., Bilen C., Kilinc I., Atar C., Kilic V., Aktuna C., Aba M., Alim Y.E., Binbuga M., Kurklu O., Ceylan C., Yilmaz S., Calar S.N., Yildirim N., Yavuz G., Can B., Cesmeli E., Kus D., Kavar R.C., Cevlik A.D., Cengiz U., Turk E.G., Yilmaz I., Erdonmez H., Gursoy F., Kara H., Onsal U., Ozbek M., Kasar P.A., Ipor A., Ozen D., Olmez M., Altintas E., Toklu 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S., Alshakhs A., Ahmeidat A., Goergen N., Ali Z., Mullarkey L., Walshe R., Lewis E., Berry B., Moneim J., Mookerjee S., Christy S., Ojofeitimi O., Carroll L., Hylands A., Delaugere L.P., Reveendran D., Coulter C., Ralston C., Laverty L., Gallagher P., Ahmed A., Yeo Y.Q., Elliott D., Bennett J., Mcnamara M., Sivarajah S., Dunmore C., Aitken G., Nair M., Aly M.H., Buari M., Ahmed K., Sheikh Z., Monks M., Lehmann J., Rotimi O., Bell T., Limnatitou D., Gormley S., Taleongpong P., Patel V., Macgregor L., Amini S., Turner C., Dwyer-Hemmings L., Busuttil A., Powell J., Hensher C., Vivian F., Wcislo K., Millar Z., Hirosue S., Ogunmwonyi I., Nakakande D., Gaze H., Nirmalanantha A., Bin Amran A.M., Foulkes A., Jones N., Pillai S., Khoury G., Powell T., Maleyko I., Sangheli A., Ransome M., Isse M., Aromolaran O., Bholah H., Anbarasan J., Rehman S., Hu E., Timms S., Reynolds W., Hotchkies A., Misra V., Theocharidou L., Malik T., Janmohamed I., Carhart B., Khan A., Hullait R., Rylance A., Butt S., Leathes J., Philip Rajathasan T., Jeddy H., Kyaw H.A., Wong N., Karelia S., Clements J.M., Rainey M., Joshi N., Rahman A., Gallagher M., Rebuffa N., Abdelgalil R., Siaw Yen Lai R., Laurence N., Thomas S., Green C., Frostick R., Khera R., Povey M., Wong H.L., McCusker C., Hlukha L., Pike G., Kamel F., Thakkar R., Donaldson C., Sequeira Campos M., Armitage M.N., Hussain A.S., Hardy B., Hassan Serry M.Y., Bradbury M., Osunronbi T., Tam L.Y.C., Khanan Kaabneh A., Lawther J., Fisher P., Tribedi T., Moosa A., Ramdin A., Goble M., Downs E., Wheldon L., Baggus E., Mandal A., Nayeem A., Ahmed S., Fradley W., Wilson C., Gallagher S., Criswell T., Ward J., Mukkavilli A., Stubbs B., Fordyce W., Suchdev N., Wei Lim S., Sookramanien S.R., Chan A., Bointas G., Paul M., Ward K.L., Bagnall M., Pherwani S.A., Wang K., Mitchell L., Heyworth J., Ayyar S., Polson R., Mackenzie E., Doyle N., Habib Z., Zardab M., Sartaj F., Farooq H., Tabibi M., Drury D.J., James S.J., Barnett R., Teasdale A.B., Richardson E.T.J., Thomas D.A., Williams R.L., Najabat-Lattif H.F., Cahya E., Lou G., Coyle M., Homyer K., Zhu L.Y., Woods M., Chang J., O'Callaghan H., Suchett-Kaye I., Mihailidis T.H., Alawattegama H., Seite E., Barrett A., Riordan E., Lam W., Dowdeswell M., Mulvenna C., Awokoya O., Gurowich L., Dhera K., Hayat S., Tincknell L., Spazzapan M., Teeling F., Sysum K., Latter J., Latter M., Khan S., Guruswamy M., Beedham W., Brazier E., Elghobashy M., Bajaj M., Mann H., Etel E., Woodmass M., Hayden H., Ali Y., Husain S., Arnold A., Pedersen A.C., Cunha P., Ahmed M., Al Zawawi S., Kudva V., Theodoropoulou K., Miscampbell M., Robinson A.V., Johnston J., Dharni A., Lamb S., Westerman T., Evans E., Campbell L., Gillespie M., Cheong C.M., Kulathevanayagam K., Varghese A., Ike S.I., Chu T.S.M., Baljer B., Mogg J.A.W., Rai P., Claireaux H.A., Williams M., Smillie R., Goetz J., Appleby E., Fadipe T., Vaughan-Burleigh S., Puri G., Hussain P., Flather R., Cutler A., Pathak S., Sheldon J., Collicott T., al-Ausi M., Jovaisaite A., Shah S.M., Khalid N., Gutmann D., Davison S., Alame Y.J., Syed L., Owen W.J., Ahsan S.D., Anthony-Uzoeto U., Macleod Hall C., Zheng S., Wynter K., James C., Sapre D., Ghosh R., Baird J., Cockburn L., Blackwood O., Simpson W., Jeong S., Bishop S., Bate R., Hobson C., Adam A.H., Redclift C., Do J., Adeleye O., Poli F., Batterham A., Brown S., Parekh J.N., Clay W., Pieri K., Jackson A., Saxena A., Gurung B., Oyebola T., O'Brien F., Djeugam B., Gardezi S., Ul-Hasan S., Martin-Hernandez M.P., Sisley M., Modi S., Antakia R., Elbayouk A., Soh Y.J., Mather J., Yusuf Z., Al-Sarraf Z., Naja M., Rassool S.B., Convill J., Nikookam Y., Warsame A., Pace C., Kiandee M., Ridwan R., Carey C., Hirri F., McMillan M.J.A., Ling J.J., Pendelbury L., Kerimzade K., Tang A., Howard E.O., Humayun S., Wadsworth O.J., Tan K., Abdelhameed F., Haglund C., Radnaeva I., Hu N., Rambhatla S., Waldron D., Madahar P., Malik S., Meney L.C., Ibrahim I., Kang C.K., Chiu J.Z.J., Livie V., Ibrahim 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Gadhvi A., Grant R., Lewsey J., Morris A., Martin H., McClarty C., Sanyal S., Alsaif A., Palkhi A., Bhopal S., Vishnu K S., Papanikolaou A., Mitra A., Nur A., Ali F., Burford C., Huq T., Irwin E., Matthews L., Ngu W.S., Hosfield T., Muneeb F., Page O., Zeb E., Al-Azzawi A., McIntosh J., Vucicevic A., Hughes M., Brooks L., Fanibi B., Dixon M., Njoku P., Morris D., Jobson J., Chowdhury H., Alawode D.O.T., Wynell-Mayow W., Udayachandran V., Alsoof D., Ekert J., Joseph N., Zulkefley N., Hunt G., Christodoulou T., Wright O., Soman S., Jamal M., Beqiri S., Borgas P., Christie S., Pereira F., Browne S., Yiu J., Dworkin A., Brayley J., Palmer A., Charalambos M., Jones C.J., Toner S., Cowden R., Lee L., Nicol P., Holman O., Imtiaz M., Albert V., Leung S.P., Erotocritou M., Stroud R., Wilkin R., Thomson W., Mackee L., G N., Bei Y., Mckenna Favier S., Ibrahim A., Kler A., Reynolds L., Mohamed S.H., Majeed Y., Fakim B., Jones A., Liversedge G., Carrington Z., Windebank J., Izzarina A., Akbani U., Craven J., Aldarragi A., Harding S., Millward A., Bedford M., Gopalan V., Midgen A., Khadka P., Cheng O., Taneja S., Manobharath N., Kok J.Y., Lim D.W.E., Buick T., Boland M., Piya S., Devlin R., Fairfield C.J., George R.J., Rahi M., Zaman S., Hajiev S., Ross T., Crisp E., Thompson C., Charalambous A., Hollywood J.L., Hammond R.F.L., Matthews J., Mendonca V., Spinty J., Khan K., Cheng J., Glynn N., Muhammad U., Khan M., Anderson L., Mccormack K., Mak J., Patrawala S., Milinkovic N., Schofield R., Chauhan M., Hartley L., Hind J., Nelson L., Ratnasingham D., Whitehead T., Dimitriadis S., Marshall K., Flint E.J., Horner C., Heybourne A., Morgan H., Wickstone C., Panagiotou D., O'Connell E., Dean K., Iqbal R., Walsh L., Yu N., Rana N., Massie E., Ng J., Jung M., Lee Y.D., Harris M., White S., Boev B., Tonchev P., Prochazka V., Skalicky A., Ravn S., Ojakaar A., Dorr-Harim C., Koutserimpas C., Giraudo G., Armellini A., Ruzzenente A., Mazzeo C., De Padua C., Realis Luc A., Maroli A., Giani I., Cufari M.E., Vitali M., Gusai G.P., Quattromani R., Virgilio E., Berti S., Di Mola F.F., Papagni V., Tuminello F., Vittori L., Longheu A., Loche G.A., Braccio B., De Luca E., Resta G., Ancans G., Tamosiunas A., Petrulionis M., Andrejevic P., Stellingwerf M.E., Abdulrahman N., van de Pas K.G.H., Thomas G., Brandsma A.M., Davids J., Rottier S.J., de Roy van Zuidewijn D., Hawkins R., Ong H.I., Li Y., Desmond B., Winstanley J., Martins M., Americano M., Frade S., Senhorinho R., Peixoto R., Alves-Vale C., Lamas M., O'Connor D.B., Hoo M., Gopaul A., Scanlon K., O'Dwyer N., Jovanovic M., Panyko A., Van Vuuren S., Curchod P., Gaspar S., Imadalou L., Mutlu D., Akyol C., Uygur F.A., Eray I.C., Biyiklioglu O., Cetin M.F., Isik A.E., Karip B., Dogan H., Sarigul L., Tunc E., Aydin T., Bodur S., Karabulut K., Francis A.A., Al-hadithi A., To N., Lau I.S.F., Smith E., Mahapatra S., McAuliffe O., Imam L., Akram B., Hossaini S., Davies R., Ko M., Collins J., Pandya A., Reilly S., Archer J., Auty C., Roche C.D., Livie J., Chaudhry F.A., Ntakomyti E., Diallo R., Bylinski T., Wright J., Masiha E., Tung J., Shirazi B., Neilson A., Epton S., Patel N., Trussell S., Couldrey A., Donnelly C., Eftychiou S., Chapman, S. 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Geçim, Ie, Disçi, E, Haksal, Mc, Agarwal, T, Maxwell‐armstrong, C, Moug, Sj, Lund, Jn, Smart, Nj, Smith, Fm, Lee, Yj, Lai, Lt, Jun, Hj, Mendis, Dm, Robb, Pm, Lee, Hj, Wyche, Aab, Davis, Lt, Jayalath, Jmsn, Blefari, Nda, Goh, Yk, Beh, Yz, Dudi‐venkata, Nn, Borrow, Jl, Chua, Th, Tan, Rr, Ng, Sy, Britten‐jones, P, Pham, Hdv, Coulter‐nile, Smcj, Gosselink, Mp, Wang, Yl, Pös, M, Bartoš, M, Malý, O, Hanušová, M, Hurný, M, Riško, J, Treskoň, R, Pospíšil, M, Hlaváčová, L, Tomanová, D, Novický, R, Sobotková, K, šuta Kimle, K, Paclík, A, Jürgens‐lahnstein, J, Kristensen, Fp, Bønnerup, K, Højgaard Pedersen, J, Jepsen, Bn, Hillgaard, Tk, Erichsen, Sb, Nielsen, Cv, Madsen, Cp, Skejø, Cd, Aabling, Rr, Sørensen, J, Schöning, L, Simon, S CS, Thäwel, T, Seckler, Am, Athanasopoulos, Pg, Giuffrida, Mc, Billo, Me, Marrosu, Ag, Ruggiu, Mw, Antona, Ad, Bitonti, Mf, Romano, Fm, Pellicanò, Ga, Murru, Ml, Angelucci, Gp, Tenconi, Sm, Mura, Fa, Ruggiu, Gv, Mura, Ga, Perrone, Ba, Esposito, Lm, Marcianò, M, Aru, Ac, Figà, F, Truškovs, A, Machatschek, Mj, Wiemann, Am, Müller, Nl, Samalavicius, Ne, Poškus, E, Kryžauskas, M, Poškus, T, Looyen, R, Becker, Maj, Molder, L, Tissen, Ym, Vries, H, Smaalen, Tc, Veen, Oc, Dingemans, Sa, Brink, Lc, Vijgen, Ghej, Voort, Emf, Pool, A, Rest, Klc, Sluijpers, Nrf, Molenaar, Cjl, Gordinou de Gouberville, Mc, Mens, Ma, Hoeks, Em, Praag, Em, Smit, Mpcm, Lely, Sj, Stijns, Rch, Leow, Ty, Ruiter, A, Aarts, Cam, Jonge, J, Alqethami, Hj, Elst, Pc, Tissink, Mw, Grüter, A, Voeten, Dm, Boon, Cl, Franssen, Rjm, Jense, Mtf, Lau, Wk, Adams, Sib, Chen, Sy, Kim, Dh, Mclaughlin, Sjp, Chapman, Da, Mcintosh, Nd, Tewhaiti‐smith, J, Yam, St, Tomé, M, Santos, Tc, Lages, Rr, Sá, Tc, Bernardes, Fm, Cunha, Mf, Veiga, Dn, Loureiro, Ar, Torre, Ap, Paixão, I, Rocha, Af, Jervis, Mj, Paixão, V, Silva, Ag, Galvão, D, Mónica, I, Guimarães, N, Martins, Sf, Rocha‐melo, M, Patrício, J, Cinza, Am, Simões, J, Gonçalves, Jp, Guimarães, J, Martins, Ar, Lérias, R, Bártolo, J, Correia, Am, Martins, Pc, Jardim, Jam, Domingos, Sp, Baião, Jm, Lopes, Nmro, Jordão, Dm, Ângelo, Md, Caroço, T, Gomes, Jr, Monteiro, Rg, Colon, Lf, Dockry, É, Aly, Ak, Kang, Sj, Pentony, Ar, Piong, Cl, Eow, Sy, Kamath, Pj, Soh, Tbw, Dyer, Ah, Chee, Sy, Tan, Ixh, Jones, Mr, Al‐salihi, A, Ciubotaru, Ci, Radulović, R, Miloševic, K, Bojičić, J, Genčić, M, Janković, U, Sucháň, Pk, Dubovský, M, Sautter, Sc, Mcduling, Cr, Haller, Ml, Piotet, Lm, Kurtoglu, Gk, Sel, Ek, Isler, Vc, Demirkaya, Hc, Korkmaz, Hk, Pektas, Am, Güner, B, İşlek, Ec, Tombul, Hb, Boztuğ, Cy, Tınaz, A, Yılmaz, M, Akın, Fa, Başçavuş, M, Seker, Mc, Koçak, Zb, Karapinar, Ye, Köseoglu, E, Agca, Mh, Alim, Ye, Kürklü, Ö, Çalar, Sn, Kavar, Rc, Cevlik, Ad, Turk, Eg, Erdönmez, H, Gürsoy, F, Önsal, U, Özbek, M, Kasar, Pa, Özen, D, Ölmez, M, Sariçiçek, T, Tipi, Ou, Benli, Ge, Bilgili, Ac, Kiraz, In, Aksoy, Ab, Karagöz, E, Tümer, S, Abdulrahman, Smf, Kuyumcu, Of, Afsar, Hb, Guler, Se, Gul, Eb, Dossa, Saa, Mizan, Sr, Sahin, Az, Kilinç, A, Yumurtaci, Ö, Girit, Çagri, Yildirak, Mk, Güldag, A, Söyleyici, B, Aytin, Ye, Akay, Fe, Iskan, Ng, Sunay, Ao, Tie‐gill, T, Delaugere, Lp, Yeo, Yq, Aly, Mh, Dwyer‐hemmings, L, Bin Amran, Am, Kyaw, Ha, Clements, Jm, Wong, Hl, Armitage, Mn, Hassan Serry, My, Tam, Lyc, Sookramanien, Sr, Ward, Kl, Pherwani, Sa, Drury, Dj, James, Sj, Teasdale, Ab, Richardson, Etj, Thomas, Da, Williams, Rl, Najabat‐lattif, Hf, Zhu, Ly, Suchett‐kaye, I, Mihailidis, Th, Pedersen, Ac, Robinson, Av, Cheong, Cm, Ike, Si, Chu, Tsm, Mogg, Jaw, Claireaux, Ha, Vaughan‐burleigh, S, Al‐ausi, M, Shah, Sm, Alame, Yj, Owen, Wj, Ahsan, Sd, Anthony‐uzoeto, U, Adam, Ah, Parekh, Jn, Ul‐hasan, S, Martin‐hernandez, Mp, Soh, Yj, Al‐sarraf, Z, Rassool, Sb, Mcmillan, Mja, Ling, Jj, Howard, Eo, Wadsworth, Oj, Meney, Lc, Kang, Ck, Chiu, Jzj, Tan, Qj, Cross, Gwv, Shah, Dr, Ng, Mpe, Wong, Rtw, Rojoa, Dm, Ruddy, Cm, Hall, Jd, Hassane, Asi, Azhar, Aw, Tan, Tk, Kwak, Sy, Ong, Eh, Goolam‐mahomed, Z, Kirnon‐jackman, O, Lim, Qx, Yong, Sq, Chen, Jy, Sam, Zh, Goh, Yn, Muthukumar, Mg, Johnson, Ta, Williams, Ri, Honeyman, Si, Malcolm, Fl, Wilkerson, Ht, Chatterjee‐woolman, S, Ahmed, Wur, Kwan, Kwl, Sanders‐crook, L, Boh, Zy, English, Wj, Novotny, Sa, Chong, Jt, Lee, De, Embury‐young, Y, Clavé Llavall, A, Pang, Yl, Dellen, J, Al‐azzawi, A, Alawode, Dot, Wynell‐mayow, W, Christodoulou, T, Jones, Cj, Leung, Sp, Mohamed, Sh, Kok, Jy, Lim, Dwe, Fairfield, Cj, George, Rj, Hollywood, Jl, Hammond, Rfl, Flint, Ej, Lee, Yd, Skalický, A, Ojakäär, A, Dörr‐harim, C, Cufari, Me, Gusai, Gp, Di Mola, Ff, Loche, Ga, Stellingwerf, Me, Pas, Kgh, Brandsma, Am, Rottier, Sj, Roy van Zuidewijn, D, Ong, Hi, Alves‐vale, C, O'Connor, Db, Jovanović, M, Uygur, Fa, Eray, Ic, Çetin, Mf, Isik, Ae, Sarıgül, L, Francis, Aa, Al‐hadithi, A, Lau, Isf, Roche, Cd, and Chaudhry, Fa
Subjects: Male, renal failure, Time Factors, medicine.medical_treatment, Adult, Aged, Anti-Inflammatory Agents, Non-Steroidal, Elective Surgical Procedures, Female, Humans, Ileus, Kaplan-Meier Estimate, Middle Aged, Patient Safety, Postoperative Care, Postoperative Complications, Proportional Hazards Models, Prospective Studies, Recovery of Function, Treatment Outcome, Colectomy, Proctectomy, Anti-Inflammatory Agents, colorectal surgery, ileus, non-steroidal anti-inflammatory drugs, NSAIDS, 030230 surgery, Tumours of the digestive tract Radboud Institute for Health Sciences [Radboudumc 14], 0302 clinical medicine, Prospective cohort study, Colorectal, RISK, Hazard ratio, Acute kidney injury, Colorectal surgery, NSAID, 030220 oncology & carcinogenesis, Non-Steroidal, Cohort study, colorectal Surgery, ileus, non-steroidal anti-inflammatory drugs, medicine.medical_specialty, Non‐steroidal anti‐inflammatory drugs. NSAIDs. Postoperative ileus. Colorectal surgery, ACUTE KIDNEY INJURY, anti-inflammatory agents, 03 medical and health sciences, Internal medicine, medicine, Adverse effect, non-steroidal anti-inflammatory drugs - ileus - colorectal surgery, business.industry, medicine.disease, Settore MED/18 - Chirurgia Generale, colorectal cancer, Surgery, business
Abstract: Ileus is common after elective colorectal surgery, and is associated with increased adverse events and prolonged hospital stay. The aim was to assess the role of non-steroidal anti-inflammatory drugs (NSAIDs) for reducing ileus after surgery.A prospective multicentre cohort study was delivered by an international, student- and trainee-led collaborative group. Adult patients undergoing elective colorectal resection between January and April 2018 were included. The primary outcome was time to gastrointestinal recovery, measured using a composite measure of bowel function and tolerance to oral intake. The impact of NSAIDs was explored using Cox regression analyses, including the results of a centre-specific survey of compliance to enhanced recovery principles. Secondary safety outcomes included anastomotic leak rate and acute kidney injury.A total of 4164 patients were included, with a median age of 68 (i.q.r. 57-75) years (54·9 per cent men). Some 1153 (27·7 per cent) received NSAIDs on postoperative days 1-3, of whom 1061 (92·0 per cent) received non-selective cyclo-oxygenase inhibitors. After adjustment for baseline differences, the mean time to gastrointestinal recovery did not differ significantly between patients who received NSAIDs and those who did not (4·6 versus 4·8 days; hazard ratio 1·04, 95 per cent c.i. 0·96 to 1·12; P = 0·360). There were no significant differences in anastomotic leak rate (5·4 versus 4·6 per cent; P = 0·349) or acute kidney injury (14·3 versus 13·8 per cent; P = 0·666) between the groups. Significantly fewer patients receiving NSAIDs required strong opioid analgesia (35·3 versus 56·7 per cent; P 0·001).NSAIDs did not reduce the time for gastrointestinal recovery after colorectal surgery, but they were safe and associated with reduced postoperative opioid requirement.El ileo es frecuente tras cirugía colorrectal electiva y se asocia con un incremento de los eventos adversos y de la duración de la estancia hospitalaria. El objetivo fue evaluar el papel de los fármacos antiinflamatorios no esteroideos (non-steroidal anti-inflammatory drugs, NSAIDs) para reducir el ileo tras la cirugía. MÉTODOS: Estudio de cohortes prospectivo y multicéntrico efecuado por un grupo colaborativo dirigido por estudiantes y cirujanos en formación. Se incluyeron pacientes adultos sometidos a cirugía colorectal electiva entre enero y abril 2018. El resultado primario fue el tiempo hasta la recuperaciòn gastrointestinal, medido por una variable compuesta de función intestinal y tolerancia a la alimentación oral. El impacto de los NSAIDs se exploró utilizando un análisis de regresión de Cox, que incluía los resultados de una encuesta específica dirigida a los centros sobre la adherencia a los principios de la recuperación intensificada. Los resultados secundarios de seguridad incluyeron la dehiscencia anastomótica y la insuficiencia renal aguda.Se incluyeron 4.164 pacientes con una mediana de edad de 68 años (rango intercuartílico: 57-75; 54,9% varones). Un total de 1.153 (27,7%) pacientes fueron tratados con NSAIDs en los días postoperarorios 1-3, de los cuales 1.061 (92,0%) recibieron inhibidores no selectivos de la ciclooxigenasa. Tras los ajustes por las diferencias basales, el tiempo medio hasta la recuperación gastrointestinal no difería significativamente entre pacientes que recibieron o no recibieron NSAIDs (4,6 versus 4,8 días; cociente de riesgos instantáneos, hazard ratio, HR 1,04, i.c. del 95%: 0,96-1,12, P = 0,360). No hubo diferencias significativas en la tasa de dehiscencias anastomóticas (5,4% versus 4,6%; P = 0,349) o insuficiencia renal aguda (14,3% versus 13,8%; P = 0,666) entre los grupos. Sin embargo, significativamente menos pacientes de los que recibieron NSAIDs precisaron una analgesia intensa con opioides (35,3% versus 56,7%; P 0,001). CONCLUSIÓN: La administración de NSAIDs no redujo el tiempo hasta la recuperación gastrointestinal tras cirugía colorrectal, pero fueron seguros y se asociaron con una reducción en la necesidad de opioides postoperarorios.
Published: 2019
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39. Cardiac and Neuromuscular Features of Patients WithLMNA-Related Cardiomyopathy

Author: Giovanni, Peretto, Chiara, Di Resta, Jacopo, Perversi, Cinzia, Forleo, Lorenzo, Maggi, Luisa, Politano, Andrea, Barison, Stefano C, Previtali, Nicola, Carboni, Francesca, Brun, Elena, Pegoraro, Adele, D'Amico, Carmelo, Rodolico, Francesca, Magri, Rosa C, Manzi, Alberto, Palladino, Franco, Isola, Lorenzo, Gigli, Tiziana E, Mongini, Claudio, Semplicini, Chiara, Calore, Giulia, Ricci, Giacomo P, Comi, Lucia, Ruggiero, Enrico, Bertini, Paolo, Bonomo, Gerardo, Nigro, Nicoletta, Resta, Michele, Emdin, Stefano, Favale, Gabriele, Siciliano, Lucio, Santoro, Gianfranco, Sinagra, Giuseppe, Limongelli, Alessandro, Ambrosi, Maurizio, Ferrari, Pier G, Golzio, Paolo Della, Bella, Sara, Benedetti, Simone, Sala, Peretto, G., Di Resta, C., Perversi, J., Forleo, C., Maggi, L., Politano, L., Barison, A., Previtali, S. C., Carboni, N., Brun, F., Pegoraro, E., D'Amico, A., Rodolico, C., Magri, F., Manzi, R. C., Palladino, A., Isola, F., Gigli, L., Mongini, T. E., Semplicini, C., Calore, C., Ricci, G., Comi, G. P., Ruggiero, L., Bertini, E., Bonomo, P., Nigro, G., Resta, N., Emdin, M., Favale, S., Siciliano, G., Santoro, Lucio., Sinagra, G., Limongelli, G., Ambrosi, A., Ferrari, M., Golzio, P. G., Bella, P. D., Benedetti, S., Sala, S., Santoro, L., Peretto, Giovanni, Di Resta, Chiara, Perversi, Jacopo, Forleo, Cinzia, Maggi, Lorenzo, Politano, Luisa, Barison, Andrea, Previtali, Stefano C, Carboni, Nicola, Brun, Francesca, Pegoraro, Elena, D'Amico, Adele, Rodolico, Carmelo, Magri, Francesca, Manzi, Rosa C, Palladino, Alberto, Isola, Franco, Gigli, Lorenzo, Mongini, Tiziana E, Semplicini, Claudio, Calore, Chiara, Ricci, Giulia, Comi, Giacomo P, Ruggiero, Lucia, Bertini, Enrico, Bonomo, Paolo, Nigro, Gerardo, Resta, Nicoletta, Emdin, Michele, Favale, Stefano, Siciliano, Gabriele, Santoro, Lucio, Sinagra, Gianfranco, Limongelli, Giuseppe, Ambrosi, Alessandro, Ferrari, Maurizio, Golzio, Pier G, Bella, Paolo Della, Benedetti, Sara, and Sala, Simone
Subjects: Male, Heart disease, Cardiomyopathy, Cardiomiopatia, laminopatie, mutazioni, malattie neuromuscolari, Arrhythmias, 01 natural sciences, Muscular Dystrophies, Sudden cardiac death, LMNA, Adult, Arrhythmias, Cardiac, Atrial Fibrillation, Atrioventricular Block, Cardiomyopathies, Disease Progression, Female, Follow-Up Studies, Gait Disorders, Neurologic, Heart Failure, Heart Transplantation, Humans, Italy, Lamin Type A, Middle Aged, Prospective Studies, Respiratory Insufficiency, Mutation, 0302 clinical medicine, 030212 general & internal medicine, Muscular Dystrophie, Ejection fraction, LMNA (lamin A/C), Atrial fibrillation, General Medicine, Cardiology, Cardiac, Human, medicine.medical_specialty, Follow-Up Studie, 03 medical and health sciences, Internal medicine, Neurologic, Internal Medicine, medicine, Gait Disorders, 0101 mathematics, Neuromuscular Manifestations, Cardiomyopathie, business.industry, 010102 general mathematics, medicine.disease, Prospective Studie, Heart failure, business
Abstract: Background Mutations in the LMNA (lamin A/C) gene have been associated with neuromuscular and cardiac manifestations, but the clinical implications of these signs are not well understood. Objective To learn more about the natural history of LMNA-related disease. Design Observational study. Setting 13 clinical centers in Italy from 2000 through 2018. Patients 164 carriers of an LMNA mutation. Measurements Detailed cardiologic and neurologic evaluation at study enrollment and for a median of 10 years of follow-up. Results The median age at enrollment was 38 years, and 51% of participants were female. Neuromuscular manifestations preceded cardiac signs by a median of 11 years, but by the end of follow-up, 90% of the patients had electrical heart disease followed by structural heart disease. Overall, 10 patients (6%) died, 14 (9%) received a heart transplant, and 32 (20%) had malignant ventricular arrhythmias. Fifteen patients had gait loss, and 6 had respiratory failure. Atrial fibrillation and second- and third-degree atrioventricular block were observed, respectively, in 56% and 51% of patients with combined cardiac and neuromuscular manifestations and 37% and 33% of those with heart disease only. Limitations Some of the data were collected retrospectively. Neuromuscular manifestations were more frequent in this analysis than in previous studies. Conclusion Many patients with an LMNA mutation have neurologic symptoms by their 30s and develop progressive cardiac manifestations during the next decade. A substantial proportion of these patients will have life-threatening neurologic or cardiologic conditions. Primary funding source None.
Published: 2019
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40. Voluntary lung function screening to reveal new COPD cases in southern Italy

Author: Onofrio Resta, Giorgio Castellana, Alberto Capozzolo, Emanuela Resta, Pierluigi Intiglietta, Lorenzo Marra, Vito Liotino, Silvano Dragonieri, Pierluigi Carratù, and Maria Rosaria Vulpi
Subjects: Adult, Male, Volition, Spirometry, Vital capacity, medicine.medical_specialty, spirometry, Population, Disease, International Journal of Chronic Obstructive Pulmonary Disease, Pulmonary Disease, Chronic Obstructive, 03 medical and health sciences, 0302 clinical medicine, Predictive Value of Tests, Risk Factors, Forced Expiratory Volume, Internal medicine, Prevalence, medicine, Humans, Mass Screening, 030212 general & internal medicine, education, Lung, Lung function, Original Research, Aged, COPD, education.field_of_study, COPD underdiagnosis, medicine.diagnostic_test, business.industry, screening, Smoking, Reproducibility of Results, General Medicine, Middle Aged, Airway obstruction, medicine.disease, respiratory tract diseases, Respiratory symptom, Early Diagnosis, Italy, 030228 respiratory system, Physical therapy, Female, Smoking Cessation, business, COPD early stages
Abstract: Alberto Capozzolo, Giorgio Castellana, Silvano Dragonieri, Pierluigi Carratù, Vito Liotino, Maria Rosaria Vulpi, Lorenzo Marra, Emanuela Resta, Pierluigi Intiglietta, Onofrio Resta Department of Cardiac, Thoracic, and Vascular Science, Institute of Respiratory Disease, School of Medicine, University of Bari, Bari, Italy Background: Underdiagnosis of COPD is a relevant issue, and most frequently involves patients at early stages of the disease. Physicians do not routinely recommend smokers to undergo spirometry, unless they are symptomatic.Aims: To investigate the effectiveness of voluntary lung function screening in bringing to light patients with previously unknown COPD and to evaluate the relationships among symptoms, smoking status, and airway obstruction.Methods: A voluntary screening study for COPD was conducted during two editions of the annual Fiera del Levante (2014 and 2015), an international trade fair in Bari. Subjects were eligible for the study if they fulfilled the following inclusion criteria: age ≥35 years, smoker/ex-smoker ≥5 pack-years (PYs), or at least one chronic respiratory symptom (cough, sputum production, shortness of breath, and wheezing). A free post-β2-agonist spirometry test was performed by trained physicians for each participant using portable spirometers. Post-β2-agonist forced expiratory volume in 1 second (FEV1):forced vital capacity ratio
Published: 2017
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41. Evidence of significant difference in key covid-19 biomarkers during the italian lockdown strategy. A retrospective study on patients admitted to a hospital emergency department in northern italy

Author: Davide, Ferrari, Anna, Carobene, Andrea, Campagner, Federico, Cabitza, Eleonora, Sabetta, Daniele, Ceriotti, Chiara, Di Resta, Massimo, Locatelli, Carobene, A, Ferrari, D, Campagner, A, Cabitza, F, Sabetta, E, Ceriotti, D, Di Resta, C, and Locatelli, M
Subjects: Male, lymphocytes, Neutrophil, Aminotransferase, COVID-19, Lactate dehydrogenase, Middle Aged, WBC, Original Investigations/Commentaries, Hospitalization, Italy, neutrophils, Quarantine, laboratory parameters, Lockdown, Humans, Female, Lymphocyte, Laboratory parameter, Emergency Service, Hospital, Biomarkers, Retrospective Studies
Abstract: Summary. Background. The Lombardy region, Italy, has been severely affected by COVID-19. During the epidemic peak, in March 2020, patients needing intensive care unit treatments were approximately 10% of those infected. This fraction decreased to approximately 2% in the second part of April, and to 0.4% at the beginning of July. COVID-19 is characterized by several biochemical abnormalities whose discrepancy from normal values was associated to the severity of the disease. The aim of this retrospective study was to compare the biochemical patterns of patients during and after the pandemic peak in order to verify whether later patients were experiencing a milder COVID-19 course, as anecdotally observed by several clinicians of the same Hospital. Material and Methods. The laboratory findings of two equivalent groups of 84 patients each, admitted at the emergency department of the San Raffaele Hospital (Milan, Italy), during March and April respectively, were analyzed and compared. Results. White blood cell, platelets, lymphocytes and lactate dehydrogenase showed a statistically significant improvement (i.e. closer or within the normal clinical range) in the April group compared to March. Creatinine, C-reactive protein, Calcium and liver enzymes, were also pointing in that direction, although the differences were not significant. Discussion. The laboratory findings analyzed in this study were consistent with a milder COVID-19 course in the April group. After excluding several hypotheses, we concluded that our observation was likely the consequence of the lockdown strategy enforcement, which, by imposing social distancing and the use of respiratory protective devices, reduced viral loads upon infection. (www.actabiomedica.it).
Published: 2020

42. Immunosuppressive therapy in childhood‐onset arrhythmogenic inflammatory cardiomyopathy

Author: Sara Benedetti, Stefania Rizzo, Simone Gulletta, Anna Palmisano, Chiara Di Resta, Paolo Della Bella, Cristina Basso, Maria Pia Cicalese, Federica Barzaghi, Massimo Slavich, Simone Sala, Alessandro Aiuti, Giorgio Casari, Francesco De Cobelli, Sara Giangiobbe, Giovanni Peretto, Antonio Esposito, Peretto, Giovanni, Barzaghi, Federica, Cicalese, Maria Pia, Resta, Chiara Di, Slavich, Massimo, Benedetti, Sara, Giangiobbe, Sara, Rizzo, Stefania, Palmisano, Anna, Esposito, Antonio, De Cobelli, Francesco, Gulletta, Simone, Basso, Cristina, Casari, Giorgio, Aiuti, Alessandro, Bella, Paolo Della, and Sala, Simone
Subjects: Male, Autoimmune myocarditis, medicine.medical_specialty, 2019-20 coronavirus outbreak, Myocarditis, ARVC, childhood, immunosuppressive therapy, multidisciplinary, myocarditis, pediatric, ventricular arrhythmias, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Cardiomyopathy, 030204 cardiovascular system & hematology, Multidisciplinary team, Electrocardiography, 03 medical and health sciences, 0302 clinical medicine, Recurrence, Risk Factors, Internal medicine, Azathioprine, medicine, Clinical endpoint, Humans, 030212 general & internal medicine, Child, Arrhythmogenic Right Ventricular Dysplasia, Immunosuppression Therapy, Pediatric, business.industry, General Medicine, medicine.disease, Magnetic Resonance Imaging, Echocardiography, Prednisone, Cardiology and Cardiovascular Medicine, business, Biomarkers
Abstract: We present, to our knowledge, the first case of immunosuppressive treatment (IST) application in a 12-year old child with arrhythmogenic inflammatory cardiomyopathy (AIC) resulting from the overlap between autoimmune myocarditis and primary arrhythmogenic cardiomyopathy (AC). Indication to off-lable IST was compelling, because of recurrent durg-refractory ventricular arrhythmias. We show that IST was feasible, safe, and effective on multiple clinical endpoints, including symptoms, ventricular arrhythmia recurrences, and T-troponin release. Remarkably, all diagnostic and therapeutic strategies were worked out by a dedicated multidisciplinary team, including specialized pediatric immunologists.
Published: 2020

43. Ethanol neurotoxicity is mediated by changes in expression, surface localization and functional properties of glutamate AMPA receptors

Author: Maria Grazia Giovannini, Domenico E. Pellegrini-Giampietro, Lucia Caffino, Elisabetta Gerace, Alice Ilari, Daniele Nosi, Daniele Lana, Roberto Ciccocioppo, Fabio Fumagalli, Alessio Masi, Guido Mannaioni, Francesco Resta, Filippo Ugolini, and Daniela Buonvicino
Subjects: 0301 basic medicine, Male, Programmed cell death, Protein subunit, Glutamic Acid, AMPA receptor, Hippocampal formation, Biochemistry, Hippocampus, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Organ Culture Techniques, mental disorders, medicine, Animals, Receptors, AMPA, Rats, Wistar, Ethanol, Chemistry, musculoskeletal, neural, and ocular physiology, Antagonist, Glutamate receptor, Neurotoxicity, medicine.disease, Flow Cytometry, Cell biology, Rats, Electrophysiology, 030104 developmental biology, Gene Expression Regulation, nervous system, AMPA receptors, CA1 injury, ethanol withdrawal, organotypic hippocampal slices, scaffold proteins, Female, Excitatory Amino Acid Antagonists, 030217 neurology & neurosurgery
Abstract: Modifications in the subunit composition of AMPA receptors (AMPARs) have been linked to the transition from physiological to pathological conditions in a number of contexts, including EtOH-induced neurotoxicity. Previous work from our laboratory showed that EtOH withdrawal causes CA1 pyramidal cell death in organotypic hippocampal slices and changes in the expression of AMPARs. Here, we investigated whether changes in expression and function of AMPARs may be causal for EtOH-induced neurotoxicity. To this aim, we examined the subunit composition, localization and function of AMPARs in hippocampal slices exposed to EtOH by using western blotting, surface expression assay, confocal microscopy and electrophysiology. We found that EtOH withdrawal specifically increases GluA1 protein signal in total homogenates, but not in the post-synaptic density-enriched fraction. This is suggestive of overall increase and redistribution of AMPARs to the extrasynaptic compartment. At functional level, AMPA-induced calcium influx was unexpectedly reduced, whereas AMPA-induced current was enhanced in CA1 pyramidal neurons following EtOH withdrawal, suggesting that increased AMPAR expression may lead to cell death because of elevated excitability, and not for a direct contribution on calcium influx. Finally, the neurotoxicity caused by EtOH withdrawal was attenuated by the non-selective AMPAR antagonist 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt as well as by the selective antagonist of GluA2-lacking AMPARs 1-naphthyl acetyl spermine. We conclude that EtOH neurotoxicity involves changes in expression, surface localization and functional properties of AMPARs, and propose GluA2-lacking AMPARs as amenable specific targets for the development of neuroprotective drugs in EtOH-withdrawal syndrome.
Published: 2021

44. A new case of Smith-Kingsmore syndrome with somatic MTOR pathogenic variant expands the phenotypic spectrum to lateralized overgrowth

Author: Federica Zinali, Roberta La Selva, Paola Coppo, Anna Fusillo, Diana Carli, Carlotta Ranieri, Alessandro Mussa, Giovanni Battista Ferrero, Simona Cardaropoli, Nicoletta Resta, and Matteo Iacoviello
Subjects: 0301 basic medicine, Male, Hemimegalencephaly, Somatic cell, 030105 genetics & heredity, Biology, Germline, Chromosomes, 03 medical and health sciences, Epilepsy, Genetics, medicine, Humans, Abnormalities, Multiple, MTOR gene, Child, Genetics (clinical), PI3K/AKT/mTOR pathway, Growth Disorders, Genetic testing, Smith-Kingsmore syndrome, lateralized overgrowth, medicine.diagnostic_test, TOR Serine-Threonine Kinases, mosaic MTOR pathogenic variant, Chromosomes, Human, Pair 1, Neurodevelopmental Disorders, Phenotype, Syndrome, Chromosome, medicine.disease, 030104 developmental biology, Pair 1, Abnormalities, Multiple, Human
Abstract: Smith-Kingsmore syndrome (SKS) is a rare autosomal dominant disorder caused by heterozygous germline activating pathogenic variants in mammalian target of rapamycin (MTOR) on chromosome 1p36. A few patients with disseminated mosaicism have been described so far and they seem to display a different phenotype when compared to germline cases. Here we report the sixth case with a disseminated mosaic MTOR pathogenic variant, a 7-year-old boy with hemimegalencephaly, epilepsy, developmental delay, hypomelanosis of Ito, and lateralized overgrowth. Genetic testing revealed a pathogenic variant (c.4448G > A, p.Cys1483Tyr) in MTOR with a frequency of 32% in the DNA extracted from a skin sample, 3% in saliva and 0.46% in blood. The clinical features observed in our patient further corroborate the existence of differences in phenotypic presentation of germline and mosaic SKS cases. Moreover, lateralized overgrowth, a finding never described so far in SKS, further expands the phenotypic spectrum of SKS and allows the inclusion of MTOR pathogenic variants among the several causes of asymmetric body overgrowth.
Published: 2021

45. Risk factors for transfer from Respiratory Intermediate Care Unit to Intensive Care Unit in COVID-19

Author: Elena Capozza, Michela Dimitri, Valentina Di Lecce, Luciana Labate, E Boniello, Onofrio Resta, Giovanna Elisiana Carpagnano, Vitaliano Nicola Quaranta, Paola Pierucci, and Enrico Buonamico
Subjects: Pulmonary and Respiratory Medicine, Male, medicine.medical_specialty, Single Center, Respiratory Intermediate Care Unit, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Risk Factors, Intensive care, medicine, Humans, COVID-19 ICU Transfer Risk Factors, 030212 general & internal medicine, Respiratory system, Pandemics, Retrospective Studies, COVID-19 Management Italy, business.industry, SARS-CoV-2, Mortality rate, COVID-19, medicine.disease, Intensive care unit, Pneumonia, Intensive Care Units, 030228 respiratory system, Emergency medicine, Original Article, Observational study, business, Respiratory Insufficiency, Intermediate care
Abstract: Background Patients hospitalized for COVID-19-related pneumonia often need several degrees of ventilatory support, which are performed between Respiratory Intermediate Care Units (RICUs) and Intensive Care Units (ICUs), and which depend on the severity of acute respiratory distress syndrome. There is no firm consensus on transfer predictors from the RICU to the ICU. Methods In this retrospective observational single center study, we evaluated 96 COVID-19 patients referred to the RICU for acute respiratory failure (ARF) according to their transferal to the ICU or their stay at the RICU. We compared demographic data, baseline laboratory profile, and final clinical outcomes to identify early risk factors for transfer. Results The best predictors for transfer to the ICU were elevated C-reactive protein and lymphopenia. The mortality rate was lower in the RICU than in the ICU, where transferred patients who died were mostly younger men and with less comorbidities than those in the RICU. Conclusions Few inflammatory markers can predict the need for transfer from the RICU to the ICU. Due to the ongoing COVID-19 pandemic, we urge better clinical stratification by early and meaningful profiles in patients admitted to the RICU who are at risk of transferal to the ICU.
Published: 2020

46. Galectin-3 and neutrophil-to-lymphocyte ratio are indicative of heart remodelling and disease severity in patients with obstructive sleep apnoea

Author: Marcella Prete, Giovanna Elisiana Carpagnano, Giorgio Castellana, Onofrio Resta, Valentina Di Lecce, Vito Racanelli, Sebastiano Cicco, G. Ranieri, Lorenzo Marra, Silvano Dragonieri, Angelo Vacca, and Pierluigi Carratù
Subjects: Adult, Male, medicine.medical_specialty, Neutrophils, Galectin 3, Galectins, Polysomnography, Diastole, Severity of Illness Index, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Severity of illness, medicine, Humans, Lymphocytes, Neutrophil to lymphocyte ratio, Respiratory system, Oxygen saturation (medicine), Aged, Sleep Apnea, Obstructive, medicine.diagnostic_test, business.industry, General Medicine, Blood Proteins, Middle Aged, medicine.anatomical_structure, 030228 respiratory system, Ventricle, Cardiology, Female, business, 030217 neurology & neurosurgery
Abstract: Galectin-3, considered as a new inflammatory marker; it is increased in cardiovascular disease. We investigated Galectin-3 in relation to heart damage in patients with OSA and its role in inflammation, based on the Neutrophil-to-Lymphocyte Ratio (NLR).Sixty-three consecutive patients (45 males, 18 females, 58.60 ± 12.28 years old) were studied. According to the Apnoea-Hypopnoea Index (AHI) patients were divided into Group 1 - non-severe (AHI30) (17 males and 10 females, 59.89 ± 10.62 years old) and Group 2 - severe (AHI ≥30) (29 males and 6 females, aged 57.53 ± 13.30 years old) OSA. All patients underwent morning blood gas analysis, laboratory tests, nocturnal polygraphy, and echocardiography.Galectin-3 was significantly increased in Group 2 (p = 0.027) patients. Moreover, it was directly related to left ventricle (LV) mass, left ventricle hypertrophy and LV posterior wall diameter. Tissue Doppler septal velocity (e'), that measures wall motion, was inversely correlated to Galectin-3. Furthermore, a direct association to diastolic dysfunction, evaluated as E/e' ratio, was observed. In line with these data, a direct correlation between Galectin-3 and left atrium volume was also found. Galectin-3 and percentage of total registration time with nocturnal oxygen saturation90% (TST90) were directly correlated (p = 0.0003), while Galectin-3 and mean nocturnal SpOGalectin-3 showed a direct association to nocturnal respiratory indices and to cardiac remodelling in patients with OSA. OSA-induced inflammation may play an important role in the pathogenesis of heart damage.
Published: 2020

47. Influence of Players' Maximum Running Speed on the Team's Ranking Position at the End of the Spanish

Author: Diego Brito de Souza, Roberto López-Del Campo, Juan Del Coso, Victor Moreno-Pérez, Fabio Nevado, Ricardo Resta, and Javier M. Buldú
Subjects: Male, velocity, Health, Toxicology and Mutagenesis, lcsh:Medicine, ComputerApplications_COMPUTERSINOTHERSYSTEMS, Football, match analysis, League, Athletic Performance, Article, Running, 03 medical and health sciences, 0302 clinical medicine, Position (vector), Range (aeronautics), Statistics, Soccer, Humans, Physical Examination, Mathematics, Football players, team sports performance, lcsh:R, ComputingMilieux_PERSONALCOMPUTING, Public Health, Environmental and Occupational Health, 030229 sport sciences, Match analysis, Ranking, Match play, Seasons, exercise training, 030217 neurology & neurosurgery
Abstract: The maximum running speed that a football player can attain during match play has become one of the most popular variables to assess a player&rsquo, s physical talent. However, the influence of a player&rsquo, s maximum running speed on football performance has not yet been properly investigated. The aim of this study was to determine the influence of a player&rsquo, s peak/maximum running speed on the team&rsquo, s ranking position at the end of a national league. A second aim was to investigate differences in maximum running speed among playing positions. To fulfil this aim, the peak/maximum running speeds of 475 male professional football players were recorded for 38 fixtures of the Spanish first-division league (LaLiga) from the 2017&ndash, 2018 season (7838 data points). Players&rsquo, peak running speeds in each match were assessed with a validated multicamera tracking system and associated software (Mediacoach&reg, ). Players&rsquo, maximum running speed was established as the fastest running speed they attained during the entire season. Most players (53.5% of the total) had a maximum running speed in the range of 32.0&ndash, 33.9 km/h, with only three players (0.6%) with a maximum running speed of over 35.0 km/h. Overall, forwards were faster than defenders and both types of players were faster than midfielders (33.03 &plusmn, 1.35 &gt, 32.72 &plusmn, 1.32 &gt, 32.08 &plusmn, 1.63 km/h, p &lt, 0.001). There was no association between teams&rsquo, maximum running speed and ranking position at the end of the league (r = &minus, 0.356, p = 0.135). The correlations between teams&rsquo, maximum speeds and ranking position were low for defenders (r = &minus, 0.334, p = 0.163), midfielders (r = 0.125, p = 0.610), and forwards (r = &minus, 0.065, p = 0.791). As a result, the variance in the ranking position at the end of the season explained by a team&rsquo, s maximum speed was of only 7.5%. Finally, as an average for all teams, players&rsquo, peak running speeds remained stable at ~30.7 &plusmn, 0.6 km/h throughout the whole season. These results suggest that successful and less successful football teams have squads with players able to obtain similar maximum running speeds during match play throughout the season. Hence, players&rsquo, maximum running speeds have a poor association with the team&rsquo, s ranking position at the end of the Spanish professional national league.
Published: 2020

48. Surgical mask and N95 in healthcare workers of Covid-19 departments: clinical and social aspects

Author: Matteo, Gelardi, Valeria, Fiore, Rossana, Giancaspro, Emanuel, La Gatta, Francesca, Fortunato, Onofrio, Resta, Giovanna Elisiana, Carpagnano, Carla, Santomasi, Michela, Dimitri, Maria Pia, Foschino Barbaro, Donato, Lacedonia, Giulia, Scioscia, Laricchiuta, Antonio, and Michele, Cassano
Subjects: Adult, Male, Infectious Disease Transmission, Patient-to-Professional, Surgical masks, N95 Respirators, SARS-CoV-2, Health Personnel, N95, Masks, COVID-19, Middle Aged, Original Investigations/Commentaries, Occupational Diseases, Life quality, Quality of Life, Humans, Female, Self Report, Work Performance
Abstract: Introdution. In order to prevent or slow down the transmission of COVID-19, various public health measures have been introduced, including social distancing, environmental disinfection and the use of personal protective equipment (PPE). In this perspective, the clinical practice of healthcare professionals has changed dramatically. As a matter of fact, the use of surgical masks and N95 has significantly worsened the job performance of workers who deal directly with COVID-19 disease. Methods. The study included 116 health workers employed in the pulmonology, intensive care and infectious diseases departments of Bari and Foggia Hospital, directly involved in the healthcare of patients affected by COVID-19. Between May 1, 2020 and May 31, 2020, each participant completed an online questionnaire aimed to investigate the impact of the COVID-19 pandemic on workers’ lifestyle changes and job performances. We compared the results based on the type of mask used by each participant (surgical mask vs N95). Results. Although disturbances related to the use of the mask arose earlier in subjects who wore the N95 (p = 0.0094), healthcare workers that wore surgical masks reported a statistically higher average score for a greater number of disorders. Conclusions. This is the first study that compares the effects of the two most used PPE on the quality of life of health workers and which highlights the greater discomfort caused by surgical masks. This result brings to light a serious social problem, being surgical masks widely used in everyday life by ordinary people and non-healthcare workers. (www.actabiomedica.it)
Published: 2020

49. Sensorized Assessment of Dynamic Locomotor Imagery in People with Stroke and Healthy Subjects

Author: Gianluca Giordani, Fabiano Bini, Stefano Paolucci, Gabriella Antonucci, F. Resta, Valeria Belluscio, Stefania Santucci, Daniela De Bartolo, Giuseppe Vannozzi, Marco Iosa, Franco Marinozzi, and Giovanni Morone
Subjects: Male, 030506 rehabilitation, medicine.medical_specialty, Movement, gait, inertial sensors, instrumented movement analysis, motor imagery, nneurorehabilitation, stroke, walking, Sensory system, lcsh:Chemical technology, Biochemistry, Article, Analytical Chemistry, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Motor imagery, Physical medicine and rehabilitation, Mental chronometry, medicine, Humans, lcsh:TP1-1185, Electrical and Electronic Engineering, Ground reaction force, Instrumentation, Stroke, Neurorehabilitation, Aged, Monitoring, Physiologic, neurorehabilitation, medicine.disease, Gait, Atomic and Molecular Physics, and Optics, Healthy Volunteers, body regions, medicine.anatomical_structure, Lower Extremity, Upper limb, 0305 other medical science, Psychology, human activities, 030217 neurology & neurosurgery
Abstract: Dynamic motor imagery (dMI) is a motor imagery task associated with movements partially mimicking those mentally represented. As well as conventional motor imagery, dMI has been typically assessed by mental chronometry tasks. In this paper, an instrumented approach was proposed for quantifying the correspondence between upper and lower limb oscillatory movements performed on the spot during the dMI of walking vs. during actual walking. Magneto-inertial measurement units were used to measure limb swinging in three different groups: young adults, older adults and stroke patients. Participants were tested in four experimental conditions: (i) simple limb swinging, (ii) limb swinging while imagining to walk (dMI-task), (iii) mental chronometry task, without any movement (pure MI), (iv) actual level walking at comfortable speed. Limb swinging was characterized in terms of the angular velocity, frequency of oscillations and sinusoidal waveform. The dMI was effective at reproducing upper limb oscillations more similar to those occurring during walking for all the three groups, but some exceptions occurred for lower limbs. This finding could be related to the sensory feedback, stretch reflexes and ground reaction forces occurring for lower limbs and not for upper limbs during walking. In conclusion, the instrumented approach through wearable motion devices adds significant information to the current dMI approach, further supporting their applications in neurorehabilitation for monitoring imagery training protocols in patients with stroke.
Published: 2020
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50. The importance of maintaining the same order of performance of lung function and SNIP tests in patients with amyotrophic lateral sclerosis

Author: Giovanna Elisiana Carpagnano, Michela Dimitri, Annalisa Carlucci, Giancarlo Logroscino, Stefano Battaglia, Paola Pierucci, Nicolino Ambrosino, Onofrio Resta, Isabella Laura Simone, Vito Liotino, Silvano Dragonieri, and Pierluigi Carratù
Subjects: Male, Amyotrophic lateral sclerosis, lung function tests, motoneuron disease, neuromuscular disease, respiratory muscle, medicine.medical_specialty, Neuromuscular disease, Vital Capacity, Pulmonary function testing, Diaphragm function, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Motoneuron disease, medicine, Respiratory muscle, Humans, In patient, Lung, Lung function, business.industry, Amyotrophic Lateral Sclerosis, respiratory system, medicine.disease, Respiratory Muscles, Respiratory Function Tests, Neurology, Cough, Cardiology, Female, Neurology (clinical), business, 030217 neurology & neurosurgery
Abstract: Objective: Sniff nasal inspiratory pressure (SNIP), a useful tool for the assessment of diaphragm function in patients with Amyotrophic Lateral Sclerosis (ALS), is usually performed together with l...
Published: 2020

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