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To determine the impact of the degree of furcation involvement (FI) on the longevity of molar teeth and assess the risk variables (tooth- and patient-related factors) associated with the loss of molars (LM) in individuals treated for periodontitis and monitored in a private programme of supportive periodontal care (SPC).The present retrospective cohort study included 222 individuals with 1329 molars under a 10-year monitoring period in SPC. Periodontal clinical parameters, FI, the type of molar, pulp vitality, and other variables of interest were collected at approximately 50 days after active periodontal therapy and after 10 years. The association of tooth- and patient-related factors with LM was assessed using a multilevel Cox regression analysis.Two-hundred and thirty-five molars were extracted during the SPC period of 12.4 ± 1.9 years. Age50 years, male gender, diabetes, smoking, and non-compliance were identified as relevant patient-related factors for LM during SPC (p .05). Significant tooth-related factors for LM were bleeding on probing (BoP) and probing depth (PD) ≥5 mm, tooth non-vitality, and class II and III FI (p .05).Class III FI, tooth non-vitality, higher mean PD and BoP, age, male gender, diabetes, and smoking all strongly influenced the prognosis of molars during SPC.

The objective of this study was to evaluate the potential association between liver cirrhosis and peri-implant diseases, as well as the influence of different risk indicators on this association.This case-control study included 64 cases with liver cirrhosis and 128 controls without liver diseases that presented the same socio-demographic and economic profile. The specific inclusion criteria were the following: aged group of 35-55 years and presenting at least one osseointegrated implant functioning for5 years. A full-mouth peri-implant and periodontal examination was performed and risk variables were recorded. The association between risk variables and the occurrence of peri-implant diseases was tested through univariate analysis and multivariate logistic regression, stratified by alcohol status. Additionally, a mediation analysis was performed to examine the mediating effect of age with peri-implantitis.A high prevalence of peri-implantitis (29.7%) was observed among cases when compared to controls (18.0%). Individuals with cirrhosis presented ~2.5 higher chance of having peri-implantitis than controls (p0.001). Significant variables associated with the occurrence of peri-implantitis in the final logistic model were the following: cirrhosis, alcohol use, age (55 years), male sex, smoking, periodontitis, and number of ≤14.An important risk association between liver cirrhosis and peri-implantitis was reported. Future studies with a larger sample size controlling for the patient- and implant-related confounders are needed to better understand the link between peri-implantitis and liver cirrhosis.Cirrhosis individuals, age, and periodontitis, as well as alcohol use and smoking interaction, should be considered as potential risk indicators for peri-implantitis.

Objective Obesity is a chronic disease that negatively affects an individual’s general and oral health. The present study aimed to compare the clinical and microbiological effects of non-surgical periodontal therapy with the full mouth disinfection (FMD) protocol on obese and non-obese individuals at 9 months post-therapy. Methodology This clinical study was first submitted and approved by the Ethics Committee. Fifty-five obese patients and 39 non-obese patients with periodontitis were evaluated. The full-mouth periodontal clinical parameters, clinical attachment level (CAL), probing depth (PD), gingival index (GI), and plaque index (PI), were monitored at baseline, 3, 6, and 9 months after periodontal treatment with full mouth disinfection (FMD) protocol. The mean count of Tannerella forsythia , Porphyromonas gingivalis , Treponema Denticola , and Aggregatibacter actinomycetemcomitans was determined by quantitative polymerase chain reaction on subgingival biofilm samples. Demographic data were assessed by Chi-square test. For clinical and microbiological parameters, two-factor repeated-measures ANOVA was used. Results In both groups, periodontal therapy using the one-stage full-mouth disinfection protocol significantly improved CAL, PD, GI, and PI (p0.05). Microbial count found no major differences (p>0.05) between obese and non-obese individuals who had undergone non-surgical periodontal therapy. Conclusions Obesity did not affect the clinical and microbiological outcomes of non-surgical periodontal therapy.

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2019 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Objective To evaluate the epidemiological and microbiological aspects of the potential association between bipolar affective disorder (BAPD) and periodontitis. Methodology The present case-control study comprised 176 individuals with BAPD and 176 controls. All individuals underwent a complete full-mouth periodontal examination and microbiological sampling. Data on bleeding on probing, probing depth, and clinical attachment level in all present teeth were recorded. Quantification of total bacterial load and Aggregatibacter actinomycetemcomitans, Treponema denticola, and Porphyromonas gingivalis counts were performed through qPCR. Data were analyzed using univariate analysis, Spearman correlation and multivariate logistic regression. Results The prevalence of periodontitis was 39.7% among controls and 58.5% among individuals with BAPD (OR = 2.13; 95% CI 1.39-3.27). A. actinomycetemcomitans and P. gingivalis counts were significantly higher in individuals with BPAD and periodontitis. The final multivariate logistic regression revealed that periodontitis was strongly associated with the total bacterial load (OR = 1.91; 95% IC = 1.0-1.99; P < 0.001) and the depressive phase of BPAD (OR = 28.94; 95% IC = 4.44-177.27; P < 0.001). Conclusion BAPD was associated with increased risk for periodontitis. Individuals with BPAD presented higher levels of A. actinomycetemcomitans and P. gingivalis, suggesting that periodontitis could be a co-morbidity frequently found in individuals with BAPD. [Cunha, Fabiano A.; Cota, Luis O. M.; Neves, Fernando S.; Costa, Fernando O.] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil [Cortelli, Sheila C.; Miranda, Tais B.; Cortelli, Jose R.] Universidade de Taubaté (Unitau)

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2017 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The objective of this study was to compare the effect of one-stage full-mouth disinfection (FMD) and conventional quadrant scaling in four weekly sessions (QS) on periodontal clinical parameters and halitosis among individuals with advanced chronic periodontitis. In this randomized controlled clinical trial, 30 individuals were divided into two groups: FMD (n = 15) and QS (n = 15). The following data were collected at the baseline and 90 days after treatment: plaque index, tongue-coating index (TCI), bleeding on probing, probing depth, and clinical attachment level. Halimetry was performed by the organoleptic method, and the levels of volatile sulfur compounds (H2S and CH3SH) were measured by gas chromatography. The Chi-square, Fisher's exact, the Mann-Whitney, the McNemar, and the Wilcoxon tests were used for statistical analysis. Both groups showed statistically significant improvements in periodontal clinical parameters, reduction in TCI, organoleptic scores, and in CH3SH levels between times. However, major reduction was observed in FMD group. Non-surgical periodontal therapy, regardless of the protocol, was effective in improving periodontal clinical status of individuals, decreasing organoleptic scores and CH3SH levels between times, as well as reducing halitosis. This study contributed to the knowledge that non-surgical periodontal therapy, whether by FMD or QS, was effective in reducing halitosis in individuals with advanced chronic periodontitis. [Silveira, Juliana Oliveira; Costa, Fernando Oliveira; Dutra, Bernardo Carvalho; Miranda Cota, Luis Otavio] Univ Fed Minas Gerais, Dept Dent Clin Oral Pathol & Oral Surg, Belo Horizonte, MG, Brazil [Dutra Oliveira, Peterson Antonio; Soares Dutra Oliveira, Alcione Maria] Pontificia Univ Catolica Minas Gerais, Dept Periodontol, Belo Horizonte, MG, Brazil [Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Periodont Res Div, Dept Dent, Sao Paulo, Brazil [Costa, Fernando Oliveira] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, Antonio Carlos Ave 6627,POB 359, BR-31270901 Belo Horizonte, MG, Brazil

Made available in DSpace on 2019-09-11T20:51:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2016 ABSTRACT Objectives This cross-sectional study compared the frequency of oral periodontopathogens and H. pylori in the mouths and stomachs of obese individuals with or without periodontitis submitted to bariatric surgery. Material and Methods One hundred and fifty-four men and women aged 18-65 were conveniently distributed into four groups. Two groups were composed of individuals who underwent bariatric surgery with (BP) (n=40) and without (BNP) (n=39) periodontitis and two obese control groups with (CP) (n=35) and without (CNP) (n=40) periodontitis. The oral pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Parvimonas micra, Treponema denticola, Tannerella forsythia, Campylobacter rectus, and Helicobacter pylori were detected by a polymerase chain reaction technique using saliva, tongue and stomach biopsy samples. Results Statistical analysis demonstrated that periodontopathogens were highly frequent in the mouth (up to 91.4%). In the bariatric surgically treated group, orally, P. gingivalis, T. denticola and T. forsythia were more frequent in periodontitis, while C. rectus was more frequent in non-periodontitis subjects. Stomach biopsies also revealed the high frequency of five oral species in both candidates for bariatric surgery (91.6%) and the bariatric (83.3%) groups. H. pylori was frequently detected in the mouth (50.0%) and stomach (83.3%). In the stomach, oral species and H. pylori appeared in lower frequency in the bariatric group. Conclusions Obese individuals showed high frequencies of periodontopathogens and H. pylori in their mouths and stomachs. Bariatric surgery showed an inverse microbial effect on oral and stomach environments by revealing higher oral and lower stomach bacterial frequencies. [ABREU, Mauro Henrique Nogueira Guimarães] Universidade Federal de Minas Gerais, Brazil FRANCO, Gilson Cesar Nobre] Universidade Estadual de Ponta Grossa, Brazil CORTELLI, Sheila Cavalca; CORTELLI, José Roberto; FRANCO, Gilson Cesar Nobre; AQUINO, Davi Romeiro] Universidade de Taubaté, Brazil PATARO, André Luiz; COTA, Luis Otavio Miranda; COSTA, Fernando Oliveira] Universidade Federal de Minas Gerais, Brazil

Made available in DSpace on 2019-09-12T16:57:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2016 Universidade Federal de Minas Gerais ([UFMG) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Objective: Sense of Coherence (SOC) has been associated with perceived oral health measures, but the contribution of SOC to clinical measures is still unclear. The aim of the present cross-sectional study was to evaluate the potential association between periodontal health outcomes, such as periodontal clinical parameters and perceived periodontal health, and SOC. Materials and methods: The study sample comprised 276 individuals, aged 18-60 years, from Belo Horizonte, Brazil. Participants answered questionnaires covering sociodemographic variables, self-perceived periodontal health and SOC. Full-mouth periodontal examinations were performed. The sample was divided into three groups according to SOC score: (a) SOC1 = weak (24-46); (b) SOC2 = moderate (47-51); (c) SOC3 = strong (52-65). Multivariate analyses including appropriate logistic or linear regression models were performed to evaluate the association between periodontal health outcomes and biological, sociodemographic and behavioural variables. Results: Perceived general oral health was associated with family income bracket (p = 0.010), smoking (p = 0.004), dental flossing (p = 0.017) and SOC (weak SOC: p = 0.005). Perceived gum disease and perceived periodontal disease were associated with SOC (weak SOC: p = 0.001 and p = 0.015, respectively). Conclusions: Overall, perceived periodontal health outcomes were associated with SOC. However, no association between clinical periodontal health outcomes and SOC were observed. [Cyrino, Renata Magalhaes; Costa, Fernando Oliveira; Miranda Cota, Luis Otavio] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, Antonio Carlos Ave 6627,POB 359, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, Jose Roberto; Cortelli, Sheila Cavalca] Universidade de Taubaté (Unitau), Ctr Periodontal Res

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2019 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Objectives MicroRNAs (miRNAs) may play an important role in inflammatory response. However, the involvement of miRNAs in the pathogenesis of periodontitis is unclear. The present study aimed to compare the miRNA expression profiles in individuals with chronic (CP) or aggressive (AP) periodontitis. Materials and methods Eighteen non-smoker individuals (CP = 9 and AP = 9) without any history of systemic diseases or previous periodontal therapies were selected at the Clinics of Periodontology from the Federal University of Minas Gerais. Gingival tissue samples were collected during the initial periodontal therapy. miRNAs were isolated, and expression patterns of 754 miRNAs were assessed with a quantitative miRNA PCR array. miRNAs expression profiles were compared between CP and AP groups. Results There were no differences observed in the miRNAs expression profiles between CP and AP (p > 0.05). According to the microarray analyses, the most expressed miRNAs in both groups were hsa-miR-1274b, hsa-let-7b-5p, hsa-miR-24-3p, hsa-miR-19b-3p, hsa-miR-720, hsa-miR-126-3p, hsa-miR-17-3p and hsa-miR-21-3p. Conclusion Findings suggested no differences in miRNAs expression profiles between chronic and aggressive forms of periodontitis. The overexpression of specific miRNAs could provide insights into the pathogenesis of both forms of the disease. [Amaral, Sergio Antonucci; Fontes Pereira, Thais Santos; Ricieri Brito, Joao Artur; Gomez, Ricardo Santigo; Costa, Fernando Oliveira; Miranda Cota, Luis Otavio] Univ Fed Minas Gerais, Sch Dent, Dept Dent Clin Oral Pathol & Oral Surg, Belo Horizonte, MG, Brazil [Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div

Made available in DSpace on 2019-09-12T16:53:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2018 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objectives: Patients with diabetes have a poor oral health-related quality of life (OHRQoL). It is not clear if this situation could be changed with effective periodontal treatment. This study examined both patients with diabetes and systemically healthy individuals to discover the impact of a gingivitis treatment protocol on OHRQoL and its relation to objective periodontal parameters. Design: After ultrasonic debridement, patients were randomly assigned to an essential-oils (EO) or placebo mouthwash group. At baseline and 3 months, OHRQoL was assessed with the Oral Health and Quality of Life United Kingdom questionnaire (OHQoL UK) along with clinical, halitometric, microbiological and inflammatory objective parameters. The primary outcome was a change in OHQoL UK scores. A factor analysis was performed and the impact of the extracted quality of life factor (QLF) and its interactions with diabetes, treatment, and time on the objective parameters, were tested by multiple linear regression models (p < 0.05). Chi-Square test compared questionnaire-answering profiles (p < 0.05). Results: Combined treatment with EO provided OHQoL improvements in both systemic conditions. Positive effect of oral health status on quality of life increased in EO groups but not in placebo groups. Question I (selfconfidence) showed the greatest factorial weight, while Question A (food intake) showed the lowest factorial weight. All patients who showed OHRQoL improvements and used the EO rinse showed the lowest plaque and gingival indices and lower levels of bacteria and volatile sulfur compounds. Conclusions: OHRQoL positively changed overtime. Most effective treatment protocols would provide better improvements in OHRQoL which is related to periodontal objective measures. [Cortelli, Sheila C.; Gargioni-Filho, Antonio; Aquino, Davi R.; Scherma, Alexandre P.; Miranda, This B.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dent Sch, Nucleus Periodontal Res, Expedicionario Ernesto Pereira 110 [Costa, Fernando O.; Cota, Luis O. M.] Univ Fed Minas Gerais, Dent Sch, Periodontal Dept, Ave Presidente Antonio Carlos 6627, Belo Horizonte, MG, Brazil

Made available in DSpace on 2019-09-11T20:51:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2018 Resumo Na busca do tratamento ideal da doença periodontal varias são técnicas não-cirúrgicas que podem ser consideradas. O objetivo deste estudo foi avaliar a eficácia da técnica de desinfecção total de boca (FMD, na sigla em Inglês) por parâmetros clínicos e microbiológicos. Foram avaliados 670 indivíduos com 230 indivíduos atendendo aos critérios de seleção e divididos em dois grupos; 115 indivíduos tratados com FMD e 115 tratados com sessões semanais de raspagem e alisamento corono radicular (SRP, na sigla em Inglês). A população avaliada tinha idade média de 51,67 anos, com periodontite crônica moderada. Os sujeitos foram avaliados antes do tratamento (T1) e 90 dias após a execução da terapia (T2), quanto à profundidade de sondagem (PS), nível de inserção clínica (NIC), índice de placa (IP), índice gengival (IG) e detecção microbiana da presença de Porphyromonas gingivalis (P.g.) e Prevotella intermedia (P.i.) por método de cultura e confirmada por testes bioquímicos. Os indivíduos tratados no grupo FMD também realizaram bochechos com clorexidina 0,12% durante sete dias após o tratamento. Os resultados foram analisados utilizando o teste estatístico t de Student e o teste de qui-quadrado. Não foram observadas diferenças estatisticamente significativas para PS e NIC entre T1 e T2 em ambos os grupos. Para IG e IP observou-se diferença significativa entre os grupos. Para os parâmetros microbianos avaliados foi observada redução de P.g. e P.i., mas apenas para P.g. com uma redução significativa em ambos os grupos. A técnica FMD com a metodologia utilizada neste estudo proporcionou condições clínicas melhoradas e redução da P.g. Em indivíduos com periodontite moderada, otimizando o tempo gasto na execução terapêutica. Abstract In the search for the ideal treatment of periodontal disease various non-surgical techniques should be considered. The objective of this study was to evaluate the efficacy of full-mouth scaling (FMS) by clinical and microbiological parameters. 670 individuals were evaluated with 230 subjects meeting the selection criteria and were divided into two groups; 115 subjects treated with FMS and 115 treated with weekly sessions of scaling and root planning (SRP). The patient population had a mean age of 51.67 years, with moderate chronic periodontitis. Subjects were evaluated prior to treatment (T1) and 90 days after execution of therapy (T2), with regards to: probing depth (PD), clinical attachment level (CAL), plaque index (PI), gingival index (GI), and microbial detection for the presence of Porphyromonas gingivalis (P.g.) and Prevotella intermedia (P.i.) by culture method and confirmed by biochemical tests. Subjects treated in the FMS group also rinsed with 0.12% chlorhexidine mouthwash for seven days following treatment. The results were analyzed using statistical Student’s t-test and chi-square test. No statistically significant differences were observed for PD and CAL between T1 and T2 in both groups. For GI and PI significant difference was observed between the groups. For the evaluated microbial parameters was observed reduction of P.g. and P.i., but only for P.g. with a significant reduction in both groups. The full mouth scaling technique with the methodology used in this study provided improved clinical conditions and reduction of P.g. in subjects with moderate periodontitis, optimizing the time spent in the therapeutic execution. [Roman-Torres, Caio Vinicius G.] Universidade Metropolitana de Santos, Brazil Jimbo, Ryo] Malmo Hogskola Odontologiska Fakulteten, Sweden Romito, Giuseppe Alexandre] Universidade de São Paulo, Brazil Cortelli, José Roberto] Universidade de Taubaté, Brazil Bryington, Matthew S.] West Virginia University, United States Roman-Torres, Caio Vinicius G.; Kussaba, Sergio T.; Pimentel, Angelica Castro] Universidade Santo Amaro, Brazil

Made available in DSpace on 2019-09-12T16:56:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2018 Johnson & Johnson Consumer Inc. Background: The efficacy of several variants of essential oil mouthrinses has been studied extensively. This is the first study to compare the anti-plaque and anti-gingivitis efficacy of two marketed essential oil mouthrinses: one is an alcohol containing mouthrinse and the other one is an alcohol-free mouthrinse. Methods: This examiner-blind, parallel-group study randomized subjects to three groups: 1) Mechanical Oral Hygiene (MOH) only; 2) MOH plus Alcohol-Containing essential oil Mouthrinse (ACM); 3) MOH plus Alcohol-Free essential oil Mouthrinse (AFM). Primary endpoint was whole-mouth mean Modified Gingival Index (MGI) at six months. Secondary endpoints included whole-mouth mean MGI at one and three months, and whole-mouth mean Plaque Index (PI) and whole-mouth mean Bleeding Index (BI) at one, three and six months. Safety assessments were conducted at all time points. Results: A total of 370 subjects were enrolled; 348 subjects completed the study. After six months, subjects using essential oil mouthrinses with or without alcohol showed significant reduction (p

Made available in DSpace on 2019-09-11T20:51:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2017 Abstract Objective: Single dose of systemic antibiotics and short-term use of mouthwashes reduce bacteremia. However, the effects of a single dose of preprocedural rinse are still controversial. This study evaluated, in periodontally diseased patients, the effects of a pre-procedural mouth rinse on induced bacteremia. Material and Methods: Systemically healthy individuals with gingivitis (n=27) or periodontitis (n = 27) were randomly allocated through a sealed envelope system to: 0.12% chlorhexidine pre-procedural rinse (13 gingivitis and 13 periodontitis patients) or no rinse before dental scaling (14 gingivitis and 15 periodontitis patients). Periodontal probing depth, clinical attachment level, plaque, and gingival indices were measured and subgingival samples were collected. Blood samples were collected before dental scaling, 2 and 6 minutes after scaling. Total bacterial load and levels of P. gingivalis were determined in oral and blood samples by real-time polymerase chain reaction, while aerobic and anaerobic counts were determined by culture in blood samples. The primary outcome was the antimicrobial effect of the pre-procedural rinse. Data was compared by Mann-Whitney and Signal tests (p

Although patients with diabetes are frequently affected by periodontitis, only a few investigations have focused on gingivitis in this at-risk population. This randomized placebo-controlled clinical trial compared the response to a gingivitis treatment protocol that combined mechanical procedures and daily use of an essential oil (EO) mouthrinse between patients with and without diabetes.The whole-mouth periodontal probing depth (PD), gingival index (GI), and plaque index (PI) were monitored in gingivitis cases among systemically healthy patients (n = 60) or those with diabetes (n = 60) at baseline and 3 months after treatment. Levels of Porphyromonas gingivalis, Tannerella forsythia, Aggregatibacter actinomycetemcomitans, and total bacterial load were determined by a real-time polymerase chain reaction in intrasulci plaque samples. The volume of gingival crevicular fluid (GCF) was quantified, and interleukin-1β (IL-1β) levels were determined in GCF samples. After a full-mouth ultrasonic debridement, patients were randomly assigned to an EO or a placebo rinse for 90 days (40 mL/day). The data were analyzed through repeated-measures analysis of variance and multiple comparisons Tukey tests (P0.05).GI was more severe in the diabetes group. Diabetes impaired GI and reduced GCF volume. PD, bacterial levels, and IL-1β improved similarly in both systemic conditions. The adjunctive use of EO provided greater reductions of PI, GI, total bacterial load, T. forsythia, A. actinomycetemcomitans, and GCF volume.Response to gingivitis treatment in patients with diabetes can slightly differ from that in patients without diabetes. Daily use of an EO mouthrinse after ultrasonic debridement benefited patients with and without diabetes.

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2013 Background: Expression patterns of human beta-defensin-2 (HBD-2) mRNA or HBD-2 protein concentration and periodontal diseases have been a focus of scientific research. This study compares the salivary levels of HBD-2 protein concentration of healthy patients and patients with gingivitis and chronic periodontitis (CP) and correlates these levels with the presence of periodontopathogens. Methods: A total of 89 patients were enrolled in this study: 31 periodontally healthy, 27 with gingivitis, and 31 with CP. Plaque and gingival indices, probing depth, and clinical attachment level were measured. The presence of Campylobacter rectus, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Prevotella intermedia was evaluated qualitatively by conventional polymerase chain reaction. HBD-2 quantification in saliva was performed using an immune enzymatic assay. Frequency of periodontopathogens and HBD-2 protein concentration was assessed. Association between HBD-2 protein concentration (>= 100 pg/mL) and the simultaneous presence of one to two, three to four, or five to six periodontopathogens was tested. Results: Although periodontally healthy individuals and patients with gingivitis showed similar HBD-2 levels, the CP group displayed an increased level of HBD-2. P. gingivalis, P. intermedia, T. forsythia, and T. denticola were more prevalent in CP; however, their mere presence was not related to the increased levels of HBD-2 (Pearson correlation and multinomial logistic regression model). Conclusions: Salivary HBD-2 protein concentration was higher in patients with CP compared with healthy individuals or patients with gingivitis. These different protein concentrations were not related to the frequency of periodontopathogens. Clinical inflammatory profile had a higher impact on salivary HBD-2 levels than bacteria. [Pereira, Alexandre L.; Franco, Gilson C.; Cortelli, Sheila C.; Aquino, Davi R.; Raslan, Suzane A.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dept Periodontol, Sao Paulo, Brazil [Costa, Fernando O.] Univ Fed Minas Gerais, Dept Periodontol, Belo Horizonte, MG, Brazil

Prospective studies that investigated the influence of glycemic control in the progression of periodontitis and tooth loss during periodontal maintenance therapy (PMT) programs have not previously been reported. The aim of the present study is to evaluate associations between glycemic control status and progression of periodontitis and tooth loss among individuals during PMT.A total of 92 individuals, all recruited from a prospective cohort with 238 participants undergoing PMT, participated in this study. Diabetes control was assessed according to percentage of glycated hemoglobin (HbA1c). Individuals were matched for sex and smoking and were divided into three groups: 23 individuals with diabetes and poor glycemic control (PGC), 23 individuals with diabetes and good glycemic control (GGC), and 46 controls with no diabetes (NDC). Full-mouth periodontal examination, including bleeding on probing (BOP), probing depth (PD), and clinical attachment level, was performed at all PMT visits during a 5-year interval.Progression of periodontitis and tooth loss were significantly higher among PGC compared to GGC and NDC. The final logistic model in the final examination included: 1) for the progression of periodontitis, HbA1c ≥6.5% (odds ratio [OR] = 2.9), smoking (OR = 3.7), and BOP in30% of sites (OR = 4.1); and 2) for tooth loss, HbA1c ≥6.5% (OR = 3.1), smoking (OR = 4.1), and PD 4 to 6 mm in ≤10% of sites (OR = 3.3).PGC individuals, especially smokers, presented with a higher progression of periodontitis and tooth loss compared to NDC and GGC individuals. This result highlights the influence of glycemic control in maintaining a good periodontal status.

Made available in DSpace on 2019-09-12T16:53:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objetivo Alguns estudos anteriores mostraram que as gingipains, proteases semelhantes à tripsina produzidas por Porphyromonas gingivalis , regulam positivamente a expressão de mRNA da β defensina-2 humana (HBD-2) por meio do receptor-2 ativado por protease (PAR 2 ) em células epiteliais gengivais. O objetivo deste estudo foi investigar os níveis salivares de HBD-2 e a expressão do mRNA PAR 2 crevicular na periodontite crônica humana e avaliar se o tratamento periodontal afetou esse processo. Métodos Amostras de fluido das fendas gengivais e salivares (GCF) foram coletadas de pacientes periodontais saudáveis ​​(controle) e com periodontite crônica no início do estudo e 50 dias após o tratamento periodontal não cirúrgico. Os níveis salivares de HBD-2 e GCF TNF-α foram analisados ​​por ELISA, e o mRNA de PAR 2 no GCF foi avaliado por RT-PCR. Resultados P. gingivalis foi significativamente ( p < 0,05) mais prevalente em pacientes com periodontite crônica quando comparados aos controles. Essa prevalência diminuiu após a terapia periodontal ( p < 0,0001). O grupo controle apresentou níveis mais baixos estatisticamente significantes de expressão de HBD-2, TNF-α e PAR 2 quando comparado ao grupo com periodontite crônica. Além disso, o tratamento periodontal reduziu significativamente a expressão de PAR 2 e os níveis de HBD-2 em pacientes com periodontite crônica ( p < 0,001). Conclusões Nossos resultados sugerem que os níveis salivares de HBD-2 e a expressão de PAR 2 mRNA do GCF são maiores em indivíduos com periodontite crônica do que em indivíduos saudáveis, e que o tratamento periodontal diminui os níveis de HBD-2 e a expressão de PAR 2 . Objective: Some previous studies have shown that gingipains, trypsin-like proteases produced by Porphyromonas gingivalis, up-regulate human beta defensin-2 (HBD-2) mRNA expression through protease-activated receptor-2 (PAR(2)) in gingival epithelial cells. This study aimed at investigating salivary HBD-2 levels and crevicular PAR(2) mRNA expression in human chronic periodontitis and evaluating whether periodontal treatment affected this process. Methods: Salivary and gingival crevicular fluid (GCF) samples were collected from periodontally healthy (control) and chronic periodontitis patients at baseline and 50 days after nonsurgical periodontal treatment. Salivary HBD-2, and GCF TNF-alpha levels were analysed by ELISA, and PAR(2) mRNA at the GCF was evaluated by RT-PCR. Results: P. gingivalis was significantly (p < 0.05) more prevalent in patients with chronic periodontitis when compared to controls. This prevalence decreased after periodontal therapy (p < 0.0001). The control group showed statistically significant lower levels of HBD-2, TNF-alpha, and PAR(2) expression when compared to the chronic periodontitis group. In addition, periodontal treatment significantly reduced PAR(2) expression and HBD-2 levels in chronic periodontitis patients (p < 0.001). Conclusions: Our results suggest that salivary HBD-2 levels and PAR(2) mRNA expression from GCF are higher in subjects with chronic periodontitis than in healthy subjects, and that periodontal treatment decreases both HBD-2 levels and PAR(2) expression. (C) 2012 Elsevier Ltd. All rights reserved. [Pereira, Alexandre Lustosa; Nobre Franco, Gilson Cesar; Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Periodontol [Holzhausen, Marinella] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: The purpose of this study is to investigate the association of the periodontal risk assessment (PRA) model with the recurrence of periodontitis and tooth loss during periodontal maintenance therapy (PMT). Methods: In a prospective PMT program, 75 regular complier (RC) and 89 erratic complier (EC) patients were selected. A periodontal examination and PRA were performed after active periodontal therapy and after 3 years of PMT. Risk profiles (low, moderate, or high) of participants were evaluated, and the recurrence of periodontitis and tooth loss were analyzed using univariate and multivariate analyses. Results: RCs showed less recurrence of periodontitis and tooth loss than ECs (P

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: Recent studies have shown that being overweight or obese is associated with a higher risk of periodontitis. However, the literature offers an insufficient number of published reports regarding the effect of bariatric surgery on oral health. As such, the present study aims to determine the association between periodontal status and being over-weight/obese in prebariatric and postbariatric surgery populations of Brazil. Methods: Three hundred forty-five participants between 18 and 60 years of age, from both sexes, were grouped according to prebariatric or postbariatric surgery and underwent a complete periodontal examination. Biologic, demographic, and behavioral variables were analyzed in a Poisson regression model. Results: Considering the timing of bariatric surgery, the sample was divided into three groups: PRE-OP (preoperative, n = 133), POS-OP1 (postoperative 6 months, n = 140). Regardless of the type of surgery (PRE-OP, POS-OP1, POS-OP2), the prevalence of periodontitis proved to be high (81.45%). There was a statistically significant difference in the prevalence of periodontitis among the PRE-OP, POS-OP1, and POS-OP2 groups (P=0.040). In the Poisson regression model, after adjusting for biologic, demographic, and behavioral risk variables, only bleeding on probing remained significantly associated with the prevalence of periodontitis (P

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2011 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Universidade de Taubaté (Unitau) Background: We previously reported a higher expression of protease-activated receptor-2 (PAR(2)) together with higher interleukin (IL)-1 alpha, IL-6, IL-8, and tumor necrosis factor-alpha levels, total proteolytic activity, Porphyromonas gingivalis (Pg) prevalence, and neutrophil-protease 3 messenger RNA (mRNA) expression in patients with chronic periodontitis compared to healthy control patients. The aim of the present study is to expand this observation by considering the site level according to the presence of Pg. Methods: Microbiologic and gingival crevicular fluid samples were collected from patients with chronic periodontitis. Pg presence was evaluated by polymerase chain reaction and PAR(2) mRNA expression was evaluated by reverse-transcription polymerase chain reaction. Total proteolytic activity in the crevicular fluid was analyzed by using a specific substrate benzoylarginine nitroanilide, and the proinflammatory mediators IL-1 alpha, IL-6, IL-8, and tumor necrosis factor-alpha were analyzed by enzyme-linked immunosorbent assay. Results: In Pg-positive periodontal sites, the mean probing depth and clinical attachment level, the prevalence of bleeding on probing sites, and crevicular fluid volume were higher (P < 0.05) compared to Pg-negative sites. In addition, with the exception of IL-8, all other inflammatory mediators were positively (P < 0.05) associated with Pg presence. Pg presence was also positively associated with a higher proteolytic activity (P = 0.0037) and higher PAR(2) mRNA expression (P = 0.0271). Conclusions: We conclude that in chronic periodontitis, periodontal pockets presenting Pg show an upregulation of PAR(2) gene expression, and higher proinflammatory profile associated with advanced clinical destruction, therefore suggesting that Pg plays a pivotal role on PAR(2)-mediated periodontal inflammation in humans. J Periodontol 2011;82:1596-1601. [Fagundes, Jose Americo G.; Monoo, Leticia D.; Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Periodontol, Sao Paulo, Brazil [Euzebio Alves, Vanessa Tubero; Pannuti, Claudio Mendes; Holzhausen, Marinella] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Objective: Investigate the association between the frequency of alcohol consumption and periodontitis. Moreover, evaluate the influence of biological, behavioural, and social risk variables in this association. Methods: Sample was comprised by 542 subjects of both genders, 35-55 years of age, who underwent a complete periodontal examination, and was divided into four groups according to the frequency of alcohol use, based on alcohol use disorders identification test (AUDIT) and Cut-down, Annoyed, Guilty, Eye-opener (CAGE) instruments: (1) no or occasional alcohol use (NA), (2) moderate alcohol use (MA), (3) intense alcohol use (IA) and (4) alcohol dependence (DA). Associations between the occurrence of periodontitis and potential risk variables were analysed by univariate and multivariate logistic regression stratified by smoking status when appropriate. Results: The prevalence of periodontitis in NA, MA, IA and DA groups were 17.2%, 24.0%, 29.6% and 53%, respectively. Alcohol odds ratio (OR) estimates significantly increased with an increase in consumption frequency (DA>IA>MA>NA) and were approximately two times higher in smokers (OR = 3.43 to 7.91) compared to non-smokers (OR = 1.22 to 3.02). Conclusion: Occurrence of periodontitis among alcohol users were high and the frequency of alcohol consumption increased the odds of periodontitis incrementally mainly in smokers. [Lages, Eugenio J. P.; Costa, Fernando O.; Lages, Elizabeth M. B.; Cota, Luis O. M.; Cyrino, Renata M.] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, Antonio Carlos Ave 6627,Pampulha POB 359, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, Sheila C.; Nobre-Franco, Gilson C.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2011 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Previous reports have demonstrated increased tryptase-like proteolytic activity in the crevicular fluid of patients with periodontal disease. In the present study, we have investigated the effect of tryptase inhibition with nafamostat mesilate (NM, 6-amino-2-naphtlyl p-guanidinobenzoate dimethansulfonate) on the development of experimental periodontitis in rats. Eighty (80) male Wistar rats were randomly separated into four groups: Control group, NM group (daily 0.1 mg/kg body weight of NM, i.p.), Ligature group (ligature placed at lower right first molars), and NM+Ligature group. The amount of alveolar bone loss (ABL) around the mesial root surface of the first mandibulary molar, as well as the myeloperoxidase (MPO) activity, and total proteolytic activity [N-benzoyl-l-arginine-p-nitroanilide (BApNA) substrate] were determined at 7 and 14 days. NM led to significantly (p < 0.05) decreased ABL in animals subjected to ligature-induced periodontitis. Tryptase inhibition prevented the onset of significant ABL at 7 days of experiment (0.44 +/- 0.16 and 0.60 +/- 0.22, p > 0.05, NM+Ligature and Control, respectively) and significantly decreased the ABL at 14 days (0.97 +/- 0.17 versus 1.82 +/- 0.26, p < 0.001, NM+Ligature versus Ligature, respectively). In addition, NM significantly decreased MPO and total proteolytic activity at 14 days (p < 0.05). These data provided evidence that tryptase inhibition with NM attenuates gingival granulocyte infiltration and ABL in an experimental model of periodontitis in rats. [Holzhausen, Marinella] Univ Sao Paulo, Sch Dent, Div Periodont, Dept Stomatol, Sao Paulo, Brazil [Balejo, Rodrigo D. P.; Lara, Guilherme M.; Cortelli, Sheila C.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dept Periodontol, Fac Dent [Saad, Wilson A.] Universidade de Taubaté (Unitau), Dept Physiol & Pharmacol, Inst Biosci

Made available in DSpace on 2019-09-12T16:26:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2010 This prospective study aimed to evaluate the incidence, the underlying reasons, and the influence of predictors of risk for the occurrence of tooth loss (TL) in a program of Periodontal Maintenance Therapy (PMT). The sample was composed of 150 complier individuals diagnosed with chronic moderate-severe periodontitis who had finished active periodontal treatment and were incorporated in a program of PMT. Social, demographic, behavioral and biological variables were collected at quarterly recalls, over a 12-month period. The effect of predictors of risk of and confounding for the dependent variable TL was tested by univariate and multivariate analysis, as well as the underlying reasons and the types of teeth lost. During the monitoring period, there was a considerable improvement in periodontal clinical parameters, with a stability of periodontal status in the majority of individuals. Twenty-eight subjects (18.66%) had TL, totaling 47 lost teeth (1.4%). The underlying reasons for TL were: periodontal disease (n = 34, 72.3%), caries (n = 3, 6.4%), prosthetic reasons (n = 9, 19.2%), and endodontic reasons (n = 1, 2.1%). Additionally, subjects with 10% of sites with probing depth between 4 and 6 mm were 5 times more likely to present TL (OR = 5.13, 95% CI 2.04-12.09). In this study, the incidence of TL was small and limited to few individuals. Additionally, gender and severity of periodontitis were significantly associated with TL during the monitoring period. [Cortelli, José Roberto] Universidade de Taubaté, Brazil Vargas, Andréia Maria Duarte] Universidade Federal de Minas Gerais, Brazil Lorentz, Telma Campos Medeiros; Cota, Luís Otávio Miranda; Costa, Fernando Oliveira] Universidade Federal de Minas Gerais, Brazil

Made available in DSpace on 2019-09-12T16:26:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2009 Knowledge about the presence of some important oral pathogens is an important step in better identifying children at risk for periodontal and/or caries diseases in later life. The purpose of this study was to detect the presence of Streptococcus mutans (Sm), Aggregatibacter actinomycetemcomitans (Aa), Campylobacter rectus (Cr), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and Tannerella forsythia (Tf) in gingival biofilm samples from 196 children, and to assess whether any of these pathogens are more associated with gingival inflammation extension and the Decayed/Missing/Filled teeth (DMFT/dmft) index. The subjects presented plaque index greater than 80% and were divided in 3 groups according to the bleeding index (BI): I) Low bleeding (≤ 30%), II) Medium bleeding (31 - 59%) and III) High bleeding (≥ 60%). The presence of each pathogen was determined by PCR. The prevalence of Sm was 71.9% and the mean dmft/DMFT was 6.68. The prevalence in low, medium and high bleeding groups was 43.5%, 34.5% and 46.7% for Aa; 43.5%, 37.9%, and 36.7% for Cr; 99.1%, 100%, and 96.7% for Pg; 56.5%, 56.9%, and 66.7% for Pi; and 58.3%, 60.3%, and 56.7% for Tf, respectively. Pg (99.0%) was the most prevalent periodontal pathogen detected followed by Tf (58.7%), Pi (58.2%), Aa (41.3%) and Cr (40.8%). Our study indicated that in this high plaque index population studied, a high prevalence of Sm and high mean DMFT were observed. In addition, the presence of Pi was associated with the presence of inflammation (P < 0.05) whereas Cr was associated with periodontal health (P < 0.05). Cortelli, S.C., Department of Periodontology, University of Taubaté, SP, Brazil Cortelli, J.R., Department of Periodontology, University of Taubaté, SP, Brazil Aquino, D.R., Department of Periodontology, University of Taubaté, SP, Brazil Holzhausen, M., Department of Periodontology, University of Taubaté, SP, Brazil Franco, G.C.N., Department of Oral Biology, University of Taubaté, SP, Brazil Costa, F.d.O., Department of Periodontology, Federal University of Minas Gerais, MG, Brazil Fine, D., Department of Oral Biology, New Jersey Dental School, United States

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2009 National Institute of Dental and Craniofacial Research (NIDCR) This longitudinal study evaluated the response to periodontal treatment by subjects infected with either JP2 (n = 25) or non-JP2 (n = 25) Aggregatibacter (Actinobacillus) actinomycetemcomitans. Participants were treated during the first 4 months by receiving (i) scaling and root planing, (ii) systemic antibiotic therapy, and (iii) periodontal surgery. Probing depth (PD), clinical attachment level (CAL), and gingival and plaque indices (GI and PI, respectively) were monitored at baseline and at 12 months, along with DNA-PCR-based subgingival detection of JP2 or non-JP2 A. actinomycetemcomitans. At baseline, PD, CAL, and GI scores were statistically higher in the JP2 strain-positive group than the non-JP2-strain-positive group. At 12 months, PD, CAL, and GI scores had decreased significantly for both groups, but the reduction rates of PD and CAL were higher in the non-JP2-strain-positive group. Among JP2-strain-positive patients in the baseline, patients who remained JP2 strain positive at 12 months showed significantly higher GIs than did the patients who had lost the detectable JP2 clone. Patients who remained JP2 strain positive at 12 months appeared to be more resistant to mechanical-chemical therapy than did those who were still non-JP2 strain positive, while the elimination of JP2 A. actinomycetemcomitans remarkably diminished gingival inflammation. Early identification and elimination of the JP2 clone of A. actinomycetemcomitans will enable practitioners to effectively predict the outcome of treatments applied to periodontal patients. [Cortelli, Sheila Cavalca; Aquino, Davi Romeiro; Goncalves Roman-Torres, Caio Vinicius; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dent Res Div, Dept Periodontol [Costa, Fernando Oliveira] Univ Fed Minas Gerais, Dent Res Div, Dept Periodontol, Belo Horizonte, MG, Brazil [Kawai, Toshihisa; Ohara, Kazuhisa] Forsyth Inst, Dept Immunol, Boston, MA USA [Nobre Franco, Gilson Cesar] Universidade de Taubaté (Unitau), Dept Oral Biol, Dent Res Div

Aim: This randomized clinical trial evaluated the effects of an essential oils-containing mouthrinse for full-mouth disinfection. Material and Methods: Fifty patients were assigned to receive full-mouth disinfection with either essential oils or placebo. At baseline, 2 and 6 months of treatment the primary outcomes probing depth (PD), plaque index (PlI) and modified gingival index (MGI) were monitored. Additional monitoring included bacterial presence (by polymerase chain reaction) in subgingival, saliva and tongue samples; flows, pH, total protein and alkaline phosphatase salivary levels. The following statistics were used: anova, Student's t-test, χ2 and Kruskal–Wallis (p

Made available in DSpace on 2019-09-12T16:56:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2009 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Lorentz TCM, Cota LOM, Cortelli JR, Vargas AMD, Costa FO. Prospective study of complier individuals under periodontal maintenance therapy: analysis of clinical periodontal parameters, risk predictors and the progression of periodontitis. J Clin Periodontol 2009; 36: 58-67. doi: 10.1111/j.1600-051X.2008.01342.x. This prospective study aimed to evaluate the progression of periodontitis and the influence of risk variables among individuals attending a programme of periodontal maintenance treatment in an academic environment. A total of 150 individuals diagnosed with chronic moderate-advanced periodontitis, and who had finished active periodontal treatment, were incorporated into the periodontal maintenance therapy. Social, demographic and biological variables of interest from subjects were collected at quarterly recalls, over a 12-month period. The effect of variables of interest and confounding on the periodontal status and progression of periodontitis was tested by univariate and multivariate logistic analysis. A total of 130 subjects (86.7%) showed stable periodontal status, whereas 20 subjects (13.3%) presented periodontitis progression. Twenty-eight subjects (18.66%) presented tooth loss that resulted in a total of 47 lost teeth (1.38%). Diabetes was not found to be associated with periodontitis progression (p=0.67). Smoking was significantly associated with a greater progression of periodontitis (OR=2.7, 95% CI 1.01-7.22). Periodontal maintenance programmes in academic environment can stabilize the periodontal condition obtained after active periodontal therapy as well as control the action of risk variables for the progression of periodontitis. [Medeiros Lorentz, Telma Campos; Miranda Cota, Luis Otavio; Duarte Vargas, Andrea Maria; Costa, Fernando Oliveira] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2009 It is believed that an increased arginase activity may lead to less nitric oxide production, which consequently increases the susceptibility to bacterial infection. Considering the hypothesis that smoking may alter the arginase activity and that smoking is considered a risk factor to dental implant survival, the present study aimed at evaluating the effect of smoking on the salivary arginase activity of patients with dental implants. Salivary samples of 41 subjects were collected: ten non-smoking and with no dental implants (group A), ten non-smoking subjects with dental implants (group B), ten smoking subjects with implants (group C), and 11 smoking subjects with no dental implants (group D). The levels of salivary arginase activity were determined by the measurement of L-ornithine and expressed as mIU/mg of protein. A significant increase in the salivary arginase activity was verified in groups C (64.26 +/- 16.95) and D (49.55 +/- 10.01) compared to groups A (10.04 +/- 1.95, p = 0.00001 and p = 0.0110, groups C and D, respectively) and B (11.77 +/- 1.45, p = 0.00001 and p = 0.0147, groups C and D, respectively). No significant difference was found between groups C and D (p = 0.32). Within the limits of the present study, it can be concluded that salivary arginase activity is increased in smoking subjects with dental implants in contrast to non-smoking subjects with dental implants, therefore suggesting a possible mechanism by which cigarette smoking may lead to implant failure. The analysis of salivary arginase activity may represent an important tool to prevent implant failure in the near future. [Queiroz, D. A.; Cortelli, J. R.; Holzhausen, M.; Aquino, D. R.] Universidade de Taubaté (Unitau), Dept Dent, Sao Paulo, Brazil [Rodrigues, E.] Universidade de Taubaté (Unitau), Dept Biol, Sao Paulo, Brazil [Saad, W. A.] Universidade de Taubaté (Unitau), Physiol & Pharmacol Basic Inst Biosci, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2008 National Institute of Dental and Craniofacial Research (NIDCR) It is unclear when the initial colonization by periodontal pathogens occurs in the oral cavity. Therefore, we report here the association between specific age groups and the time when the initial colonization by periodontal pathogens occurs in the oral cavity in such groups. Findings are based on an epidemiological analysis of the prevalence of five periodontal pathogens in the oral cavities of a wide range of age populations, from newborn to elderly, who were randomly selected in a geographic region of Brazil. These periodontal pathogens include Campylobacter rectus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Tannerella forsythia and were analyzed in the bacterial samples isolated from gingival sulcus, the dorsum of the tongue, and cheek mucosa of diverse age groups, using a bacterial DNA-specific PCR method. Results indicated that there are distinct age-related groups where initial colonization by the five periodontal pathogens examined in this study can be detected and that the presence of teeth is a permissive factor for colonization by P. gingivalis, P. intermedia, and T. forsythia. Although it remains unclear exactly how or when target pathogens colonize healthy subjects, an understanding of age-related groups does provide a potentially useful tool in the early detection and prevention of periodontitis in healthy individuals. [Cortelli, Jose Roberto; Aquino, Davi Rorneiro; Cortelli, Sheila Cavalca; Fernandes, Carnila Borges; de Carvalho-Filho, Jonas; Franco, Gilson Cesar Nobre] Universidade de Taubaté (Unitau), Dept Dent [Costa, Fernando Oliveira] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil [Kawai, Toshihisa] Forsyth Inst, Dept Immunol, Boston, MA USA

Made available in DSpace on 2019-09-12T16:53:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2008 Objective. To examine the histomorphologic and histomorphometric features of tissue from 3 unrelated families with hereditary gingival fibromatosis (HGF). Study design. Twelve affected individuals from 3 HGF families and 3 control subjects were evaluated. Gingival samples were fixed in formalin and embedded in paraffin for hematoxylin and eosin stain to count the number of fibroblast and inflammatory cells. Sirius red staining was performed to quantitate the amount of collagen present. Results. Histomorphologic analysis of HGF showed extension of epithelial rete ridges into the underlying lamina propria and the presence of collagen bundles in the connective tissue. Analysis of the mean area fraction of collagen showed that there were significant increases in the collagen fraction for all HGF types compared with control subjects (P < .05). There were significant increases in the number of fibroblasts for HGFa and HGFb compared with control subjects (P < .05). The number of fibroblasts for HGFc were similar to that for control subjects. Conclusions. The collagen fraction was significantly greater in all HGF types compared with controls. The number of fibroblasts was significantly increased in 2 of the 3 HGF types compared with controls. These data indicate that different mechanisms may be responsible for tissue enlargement in different forms of HGF. [Kather, Jorge; Lodi Salgado, Ulisses Fernando; Cortelli, Jose Roberto; Pallos, Debora] Universidade de Taubaté (Unitau), Dept Periodontol [Castillo Salgado, Miguel Angel] Sao Paulo State Univ, Sch Dent, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2015 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Antecedentes e Objetivo Estudos prospectivos que investigaram a influência de procedimentos cirúrgicos e não cirúrgicos na recorrência de periodontite e perda dentária em programas de terapia de manutenção periodontal (PMT) não foram relatados anteriormente. O objetivo deste estudo foi avaliar longitudinalmente a recorrência da periodontite em indivíduos compliers regulares (RC) e compliers irregulares (CI) submetidos a procedimentos cirúrgicos e não cirúrgicos durante 5 anos em um programa de PMT. Materiais e métodos Participaram deste estudo 212 indivíduos. O exame periodontal da boca completa, incluindo sangramento na sondagem, profundidade de sondagem e nível de inserção clínica foram determinados em todas as visitas PMT durante 5 anos. A recorrência da periodontite foi avaliada em indivíduos com RC e CI submetidos a procedimentos cirúrgicos e não cirúrgicos na PMT. As influências das variáveis ​​de risco de interesse foram testadas por meio de análise univariada e regressão logística multivariada. Resultados A recorrência de periodontite foi significativamente menor entre RC quando comparada com CI. Indivíduos com recorrência de periodontite e submetidos a procedimentos cirúrgicos apresentaram maior profundidade de sondagem e perda de inserção clínica do que aqueles que receberam procedimentos não cirúrgicos. A recorrência da periodontite foi maior em indivíduos submetidos a procedimentos cirúrgicos e complacência irregular durante a PMT. Conclusões A adesão irregular e os procedimentos cirúrgicos em indivíduos submetidos à PMT apresentaram maiores taxas de recorrência de periodontite quando comparados a pacientes regulares submetidos a procedimentos não cirúrgicos. Background and Objective Prospective studies that investigated the influence of surgical and nonsurgical procedures in the recurrence of periodontitis and tooth loss in periodontal maintenance therapy (PMT) programs have not been previously reported. The objective of this study was to evaluate longitudinally the recurrence of periodontitis in regular compliers (RC) and irregular compliers (IC) individuals undergoing surgical and non-surgical procedures over 5 years in a program of PMT. Materials and Methods A total of 212 individuals participated in this study. Full-mouth periodontal examination including bleeding on probing, probing depth, and clinical attachment level were determined at all PMT visits over 5 years. The recurrence of periodontitis was evaluated in RC and IC individuals undergoing surgical and non-surgical procedures in PMT. The influences of risk variables of interest were tested through univariate analysis and multivariate logistic regression. Results Recurrence of periodontitis was significantly lower among RC when compared to IC. Individuals with recurrence of periodontitis and undergoing surgical procedures showed higher probing depth and clinical attachment loss than those who received non-surgical procedures. Recurrence of periodontitis was higher in individual undergoing surgical procedures and irregular compliance during PMT. Conclusions Irregular compliance and surgical procedures in individuals undergoing PMT presented higher rates of recurrence of periodontitis when compared to regular compliant patients undergoing non-surgical procedures. [Costa, Fernando Oliveira; Miranda Cota, Luis Otavio; Cyrino, Renata Magalhaes; Pereira Lages, Eugenio Jose] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, Belo Horizonte, MG, Brazil [Cortelli, Jose Roberto; Cortelli, Sheila Cavalca] Universidade de Taubaté (Unitau), Ctr Periodontal Res, Sao Paulo, Brazil [Lima Oliveira, Ana Paula] Univ Fed Uberlandia, Dept Periodontol, Sch Dent, BR-38400 Uberlandia, MG, Brazil

Made available in DSpace on 2019-09-12T16:53:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2015 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Purposes: The primary aim of this cross-sectional study was to compare the levels of red complex bacteria between Afro-Brazilian and non Afro-Brazilian cohort. The secondary aim was to compare the distribution of both Aggregatibacter actinomycetemcomitans serotype b and its JP2 strains among participants who harboured this bacterial species. Methods: A total of 84 individuals were included in this study: 42 Afro-descendants (mean age 35.9 +/- 13.1 years) and 42 non-Afro-descendants (mean age 36.2 +/- 13.1 years) matched (1:1) by periodontal diagnosis, age and gender. All participants received clinical examinations of periodontal pocket depth, clinical attachment level, and plaque and gingival indices. Subgingival samples were taken for microbial analysis. First, genomic DNA (gDNA) was extracted and purified and the quantification of total number of bacterial cells, A. actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola was carried out by qPCR. Then, A. actinomyceterncomitans strains were classified according to serotype b and JP2 profiles by conventional PCR. Results: Clinically, mean PD, mean CAL and percentage of CAL >= 3 mm differed between groups (Student's t-test p < 0.05). The levels Of red complex bacteria between Afro-Brazilian and non-Afro-Brazilian populations were similar. The exception was verified to A. actinomycetemcomitans showing significantly higher levels among Afro-Brazilian descendants in comparison to non-Afro-Brazilian descendants. Afro-Brazilian descendants were clearly infected by more virulent serotype b and JP2 strains. Conclusions: Despite no statistically significant differences related to the red complex species, Afro-Brazilian descendants harboured higher levels of A. actinomycetemcomitans. Also, our findings confirm that Afro-descendant populations are preferably colonised by A. actinomycetemcomitans serotype b as well as JP2 strains. (C) 2015 Elsevier Ltd. All rights reserved. [Neris, Murilo de Araujo] Universidade de Taubaté (Unitau), Sch Dent, Dept Periodontol [Cortelli, Sheila Cavalca; Aquino, Davi Romeiro; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Sch Dent, Nucleus Periodontal Res [de Miranda, Tais Browne] Universidade de Taubaté (Unitau), Dept Biol [Costa, Fernando de Oliveira] Univ Fed Minas Gerais, Sch Dent, Periodontal Dept, BR-31270901 Belo Horizonte, MG, Brazil

Made available in DSpace on 2019-09-12T16:56:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2005 Objectives: Previous studies suggest differences between geographically and racially distinct populations in the prevalence of periodontopathic bacteria as well as greater periodontal destruction associated with infection by highly leucotoxic Actinobacillus actinomycetemcomitans. The present study examined these hypotheses in Brazilians with aggressive or chronic periodontitis. Materials and Methods: Clinical, radiographical, and microbiological assessments were performed on 25 aggressive periodontitis and 178 chronic periodontitis patients including 71 males and 132 females, 15-69 years of age. Results: The prevalence of Porphyromonas gingivalis was similar to that of other South American populations. The prevalence of A. actinomycetemcomitans and its highly leucotoxic subgroup was higher in Brazilians. Highly leucotoxic A. actinomycetemcomitans was more prevalent in aggressive periodontitis (chi(2) = 527.83) and positively associated with deep pockets (46 mm, chi(2) = 18.26) and young age (< 29 years, chi(2) = 18.68). Greater mean attachment loss was found in subjects with highly leucotoxic A. actinomycetemcomitans than in subjects with minimally leucotoxic (p = 0.0029) or subjects not infected (p = 0.0001). Conclusion: These data support the hypothesis of differences between populations in the prevalence of periodontopathic bacteria and of greater attachment loss in sites infected with highly leucotoxic A. actinomycetemcomitans. Detection of highly leucotoxic A. actinomycetemcomitans in children and adolescents may be a useful marker for aggressive periodontitis. SUNY Buffalo, Sch Dent Med, Buffalo, NY 14214 USA; Universidade de Taubaté (Unitau), Sao Paulo, Brazil

The use of whole saliva has shown to be promising in detecting Actinobacillus actinomycetemcomitans out of the subgingival environment. The objective of the present study was to evaluate the use of unstimulated saliva in detecting A. actinomycetemcomitans and to compare the subgingival and extracrevicular occurrence of this pathogen in Brazilian subjects with chronic periodontitis.Sixty-six patients (mean age 38.01 9.28 years) with advanced generalized chronic periodontitis were sampled. Subgingival plaque samples were collected from eight sites per patient representing the two deepest sites of each quadrant. Samples of the mucous surfaces, including dorsal surface of the tongue and cheek, were collected with a sterile swab and placed in a microtube containing a reduced solution. Samples of unstimulated saliva were also collected in sterile tubes and 0.1 ml of whole saliva was diluted in 1 ml of reduced solution. The presence of A. actionomycetemcomitans was established using bacterial culture in trypticase soy bacitracin vancomycin selective media. Polymerase chain reaction (PCR) was used to differentiate highly from minimally leukotoxic strains in patients who presented A. actinomycetemcomitans in at least two sampled sites.A. actinomycetemcomitans was isolated from 63.63% of subgingival samples, 56.06% of saliva samples, and 45.45% of samples from mucous surfaces. No statistical difference was observed between subgingival and salivary occurrence of the microorganism. Linear regression showed an association between subgingival plaque and saliva (r(2) = 0.897; P = 0.015) and mucous membrane and saliva (r(2) = 0.152; P = 0.024). The same A. actinomycetemcomitans leukotoxic profile was observed in all sampled sites for a given patient.These results suggest that in advanced periodontitis, unstimulated saliva is representative of pooled subgingival plaque samples and its use is appropriate in the oral detection of A. actinomycetemcomitans.

Hereditary gingival fibromatosis (HGF) is a rare, autosomal dominant form of gingival overgrowth. Affected individuals have a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of the oral masticatory mucosa. Genetic loci for autosomal dominant forms of HGF have been localized to chromosome 2p21-p22 (HGF1) and chromosome 5q13-q22 (HGF2). To identify the gene responsible for HGF1, we extended genetic linkage studies to refine the chromosome 2p21-p22 candidate interval to approximately 2.3 Mb. Development of an integrated physical and genetic map of the interval identified 16 genes. Sequencing of these genes, in affected and unaffected HGF1 family members, identified a mutation in the Son of sevenless-1 (SOS1) gene in affected individuals. In this report, we describe the genomic structure of the SOS1 gene and present evidence that insertion of a cytosine between nucleotides 126,142 and 126,143 in codon 1083 of the SOS1 gene is responsible for HGF1. This insertion mutation, which segregates in a dominant manner over four generations, introduces a frameshift and creates a premature stop codon, abolishing four functionally important proline-rich SH3 binding domains normally present in the carboxyl-terminal region of the SOS1 protein. The resultant protein chimera contains the wild-type SOS1 protein for the N-terminal amino acids 1-1083 fused to a novel 22-amino acid carboxyl terminus. Similar SOS1 deletion constructs are functional in animal models, and a transgenic mouse construct with a comparable SOS1 chimera produces a phenotype with skin hypertrophy. Clarification of the functional role of this SOS1 mutant has implications for understanding other forms of gingival fibromatosis and corrective gingival-tissue management.

Made available in DSpace on 2019-09-12T16:53:47Z (GMT). No. of bitstreams: 0 Previous issue date: 1998 National Institute of Dental and Craniofacial Research (NIDCR) Gingival fibromatosis is characterized by a slowly progressive benign enlargement of the oral gingival tissues. The condition results in the teeth being partially or totally engulfed by keratinized gingiva, causing aesthetic and functional problems. Both genetic and pharmacologically induced forms of gingival fibromatosis are known. The most common genetic form, hereditary gingival fibromatosis (HGF), is usually transmitted as an autosomal dominant trait, although sporadic cases are common and autosomal recessive inheritance has been reported. The genetic basis of gingival fibromatosis is unknown. We identified an extended family (n = 32) segregating an autosomal dominant form of isolated gingival fibromatosis. Using a genomewide search strategy, we identified genetic linkage (Z(max) = 5.05, theta = .00) for the HGF phenotype to polymorphic markers in the genetic region of chromosome 2p21 bounded by the loci D2S1788 and D2S441. This is the first report of linkage for isolated HGF, and the findings have implications for identification of the underlying genetic basis of gingival fibromatosis. Wake Forest Univ, Bowman Gray Sch Med, Dept Pediat, Med Genet Sect, Winston Salem, NC 27157 USA; Wake Forest Univ, Bowman Gray Sch Med, Dept Dent, Winston Salem, NC 27157 USA; Wake Forest Univ, Bowman Gray Sch Med, Dept Biochem, Winston Salem, NC 27157 USA; Universidade de Taubaté (Unitau), Dept Dent, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:53:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2013 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background. To assess changes in gingival status of renal transplant subjects under immunosuppressive regimens based on cyclosporine (CsA), tacrolimus (Tcr), and sirolimus (Sir). Methods. From a sample of 135 subjects (baseline examination [BE]), 89 without periodontal treatment, who maintained the immunosuppressive regimen based on the same main agent (CsA=23, Tcr=31, and Sir=35), were reexamined after 44 months (follow-up examination [FE]). Demographic, pharmacologic, and periodontal variables were collected and gingival overgrowth (GO) was assessed by visual examination. Results. In Tcr and CsA groups, although not significant, occurrence of GO decreased (CsA [BE=56.5% and FE=34.8%; P=0.063] and Tcr [BE=19.4% and FE=12.9%; P=0.500]). In addition, the severity of GO decreased significantly in CsA group (mean score BE=10.29-7.70 and mean score FE=0.78-1.38; P=0.003). In Sir group, GO decreased from 17.1% (BE) to 0.0% (FE) (P=not applicable). In total sample, GO was associated with papillary bleeding index (P=0.001) and concomitant use of calcium channel blockers (P=0.029); in CsA and Tcr groups, GO was associated with papillary bleeding index (P=0.029 and 0.033, respectively). Conclusions. There was no incidence of GO, and a decrease in the occurrence and severity of GO was significant in total sample. This decrease can be attributed to changes in pharmacologic and periodontal variables over this period of time. [Costa, Lidiane C. M.; Costa, Fernando Oliveira; Cota, Luis O. M.] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, Sheila C.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div

Made available in DSpace on 2019-09-12T16:53:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2013 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objectives: This study tested the hypotheses that there is: (1) higher bacterial frequency in peri-implantitis/periodontitis, followed by mucositis/gingivitis and peri-implant/periodontal health; (2) similar bacterial frequency between comparable peri-implant and periodontal clinical statuses. Design of study: The presence of Porphyromonas gingivalis, Tannerella forsythia, Campylobacter rectus, Prevotella intermedia, Treponema denticola and Aggregatibacter actinomycetemcomitans was evaluated in pen-implant (n = 53) and periodontal (n = 53) health; mucositis (n = 50), gingivitis (n = 50), peri-implantitis (n = 50) and periodontitis (n = 50). Results: The pattern of peri-implant bacterial frequency was not as expected (peri-implantitis > mucositis > health). Except for P. intermedia (p > 0.05), bacterial frequency was higher in peri-implantitis than health (p < 0.05). The frequency of P.gingivalis and red complex species were higher in peri-implantitis than mucositis (p < 0.05). In periodontal samples, T. forsythia and T. denticola showed the expected pattern of frequency (periodontitis > gingivitis > health). The frequencies of C. rectus and T.forsythia were higher in healthy teeth/gingivitis than healthy implants/mucositis, respectively (p < 0.05). The frequency of P. gingivalis and A. actinomycetemcomitans were similar between periodontitis and peri-implantitis (p > 0.05) while all other species occurrences were higher in periodontitis than peri-implantitis (p < 0.05). Conclusions: Bacterial frequency increased from pen-implant/periodontal health to peri-implantitis/periodontitis but not from mucositis/gingivitis to peri-implantitis/periodontitis. There was a trend towards higher bacterial frequency in teeth than implants. (C) 2012 Elsevier Ltd. All rights reserved. [Romeiro, Rogerio Lima; Orzechowski, Paulo Roberto] Universidade de Taubaté (Unitau) , Dept Periodontol, BR-12020330 Taubate, SP, Brazil [Costa, Fernando Oliveira] Univ Fed Minas Gerais, Dept Periodontol, BR-31270901 Belo Horizonte, MG, Brazil [Araujo, Vera Cavalcanti] Res Ctr, BR-13045755 Campinas, SP, Brazil [Duarte, Poliana Mendes] Univ Guarulhos, Dept Periodontol, BR-07023070 Guarulhos, SP, Brazil

Made available in DSpace on 2019-09-12T16:57:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2013 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Este estudo transversal investigou associações de exposição cumulativa ao tabagismo e duração da cessação do tabagismo com periodontite e avaliou os efeitos de variáveis ​​de risco biológico, comportamental e social sobre essas associações. A amostra foi composta por 705 adultos de ambos os sexos (idade: 35 a 65 anos), submetidos a exame periodontal de boca cheia. Os indivíduos foram classificados de acordo com o status de fumantes como não fumantes, ex-fumantes e fumantes atuais, e análises univariadas e multivariadas foram usadas para avaliar associações entre prevalência de periodontite e possíveis variáveis ​​de risco. As taxas de periodontite entre não fumantes, ex-fumantes e fumantes atuais foram de 25,6%, 29,3% e 45,1%, respectivamente. Após o ajuste para outras variáveis ​​de risco periodontais, o odds ratio (intervalo de confiança de 95%) para periodontite foi de 3. 09 (1,98-4,92) para ex-fumantes e 5,24 (2,61-8,97) para fumantes atuais. Foi observada uma relação dose-resposta significativa entre maços-ano de tabagismo e prevalência de periodontite, assim como uma diminuição significativa no risco de periodontite com o aumento dos anos de cessação do tabagismo. A exposição cumulativa ao fumo e a duração da cessação do tabagismo foram significativamente associadas à periodontite. This cross-sectional study investigated associations of cumulative smoking exposure and duration of smoking cessation with periodontitis and evaluated the effects of biological, behavioral, and social risk variables on these associations. The sample comprised 705 adults of both sexes (age, 35-65 years) who underwent a full-mouth periodontal examination. Subjects were classified according to smoking status as nonsmokers, former smokers, and current smokers, and univariate and multivariate analysis was used to evaluate associations between periodontitis prevalence and potential risk variables. The rates of periodontitis among nonsmokers, former smokers, and current smokers were 25.6%, 29.3%, and 45.1% respectively. After adjusting for other periodontal risk variables the odds ratio (95% confidence interval) for periodontitis was 3.09 (1.98-4.92) for former smokers and 5.24 (2.61-8.97) for current smokers. A significant dose-response relationship between pack-years of smoking and periodontitis prevalence was observed, as was a significant decrease in the risk of periodontitis as years of smoking cessation increased. Cumulative smoking exposure and duration of smoking cessation were significantly associated with periodontitis. [Costa, Fernando O.; Cota, Luis O. M.; Lages, Eugenio J. P.; Cyrino, Renata M.] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil [Oliveira, Alcione M. S. D.; Oliveira, Peterson A. D.] Pontificia Univ Catolica Minas Gerais, Fac Dent, Belo Horizonte, MG, Brazil [Cortelli, Jose R.] Universidade de Taubaté (Unitau), Sch Dent, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Johnson and Johnson Consumer Companies Inc. This cross-over study was conducted to assess the germ-killing efficacy of an essential oil mouthrinse (EOM) by determining the blood levels of microorganisms associated with induced bacteremia and investigating the prevalence of this event in Brazilians with mild-to-moderate gingivitis. Thirty four (31.19%) subjects positive for bacteremia induced by chewing a ration of apple were enrolled out of 109 screened subjects (50 males and 59 females). A difference of at least 10 colony forming units between the pre- and post-insult blood samples was defined as a positive result. For the following two weeks patients underwent a toothbrush plus fluoride dentifrice normalization period, and were then scheduled for the Phase I protocol as follows. At baseline I, subjects were instructed to chew a new apple ration, had new blood samples taken before and after this oral stimulus, and were randomly assigned to an experimental essential oil (n = 17) or placebo (P) mouthrinse (n = 17) treatment for 2 weeks. These procedures were repeated at the end of Phase I and then followed by a two-week wash-out period (tooth brushing with fluoride dentifrice). Bacteremia was again induced at baseline and at the end of Phase II, when subjects were crossed-over to the other EOM or placebo groups. Bacterial count differences between baseline and 2-week post-treatment (EOM versus P) in the blood samples collected were assessed by analysis of covariance. Mean aerobic counts decreased by 45.8%, whereas mean anaerobic counts decreased by 63.3% after EOM treatment. After the P treatment, aerobic bacteria increased by 28.4% and anaerobic bacteria decreased by 18.5%. This study validated this novel methodology and showed that the germ-killing action of EOM significantly reduced bacteremia. [Ricci-Nittel, Danette; Zhang, Paul; Barata de Araujo, Marcelo Werneck] Div Johnson & Johnson Consumer Companies Inc, Johnson & Johnson Consumer & Personal Prod Worldw, Morris Plains, NJ USA [Cortelli, Jose Roberto; Cago, Karina; Aquino, Davi Romeiro; Cortelli, Sheila Cavalca] Universidade de Taubaté (Unitau), Sch Dent, Dept Periodontol, Nucleus Periodontal Res NUPER

This 6-month, examiner-blind, single-center, randomized, parallel group clinical trial compared the antiplaque and antigingivitis effects of an essential oil-containing mouthrinse with zinc chloride and sodium fluoride (EO) to a 0.05% cetylpyridinium chloride-containing mouthrinse (CPC) also with fluoride.Four hundred and eight gingivitis subjects were monitored for the primary outcomes of modified Gingival Index (MGI) and Plaque Index (PI) at baseline and 3 and 6 months. Subjects were randomly assigned to 6-month twice a day unsupervised use of EO, CPC, or negative control rinse in conjunction with normal brushing and flossing.EO was always better than CPC at 3 and 6 months considering all parameters. All benefits allowed by EO increased from 3 to 6 months. CPC was better than the negative control at 3 and 6 months with respect to whole mouth plaque, and the proportion of more severe sites (baseline scores ≥ 3) in PI and MGI. At 6 months, CPC did not differ from negative control in relation to whole mouth MGI reduction, proximal MGI reduction, and percentages of sites improved over baseline in PI and MGI.This new EO mouthrinse provided superior clinical benefits to CPC and demonstrated increasing plaque and gingivitis reductions over 6 months. Our findings support the regular long-term use of the EO mouthrinse and selection over a 0.05% CPC rinse for better efficacy.

Made available in DSpace on 2019-09-12T16:53:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) National Institutes of Health Harvard Catalyst Pilot Grant We report the age-related prevalence of red complex periodontal pathogens, , , and , along with four strains of orange complex pathogens. The bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (PCR). was not detected in any site of any subject in the two groups tested. However, was not only found in the 6-13 years age group, but also in edentulous newborns at a relatively high prevalence, indicating non-dentition-related colonization by . , , , , and were found in the oral cavity of most subjects belonging to the 6-13 years age group compared to newborns. This suggested a pronounced association between these colonizing bacteria and the presence of teeth. There was also a strong relation between and for their prevalence in the subgingival sulcus of the 6-13 years age group ( < 0.0001), but not in the other sites tested, suggesting that the colonization of dentition-related may be associated with the increased prevalence of non-dentition-related in the subgingival sulcus. Overall, these results suggest that dentition is a key determinant of bacterial colonization, especially orange complex bacteria and the red complex bacterium . [Cortelli, J. R.] Universidade de Taubaté (Unitau), Dept Periodontol & Prevent Dent, Dent Res Div, BR-12020040 Taubate, SP, Brazil [Fernandes, C. B.] Universidade de Taubaté (Unitau), Dept Periodontol, Dent Res Div, BR-12020040 Taubate, SP, Brazil [Costa, F. O.] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, S. C.] Universidade de Taubaté (Unitau), Nucleus Periodontolal Res, Dent Res Div, BR-12020040 Taubate, SP, Brazil [Kajiya, M.] Forsyth Inst, Dept Immunol, Cambridge, MA 02142 USA [Howell, S. C.] Harvard Univ, Dept Oral Med Infect & Immun, Sch Dent Med, Boston, MA 02115 USA [Kawai, T.] Harvard Univ, Div Endodont, Sch Dent Med, Boston, MA 02115 USA

Made available in DSpace on 2019-09-12T16:56:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: This prospective study aims to evaluate and compare the periodontal status, periodontitis progression, tooth loss, and influence of predictable risk variables of two periodontal maintenance therapy programs over a 12-month period. Methods: A total of 288 individuals diagnosed with chronic moderate-to-advanced periodontitis, who had finished active periodontal treatment, were evaluated in a public academic environment (AG) (n = 138), as well as in a private clinic (PG) (n = 150). A full-mouth periodontal examination was performed at baseline and at quadrimestral recalls, evaluating plaque index, probing depth, clinical attachment level, furcation involvement, bleeding on probing (BOP), and suppuration. Individuals' social, demographic, and biologic data, as well as compliance with recalls, were recorded. The effect of variables of interest and confounders were tested by univariate and multivariate analysis. Results: The PG demonstrated lower rates of periodontitis progression and tooth loss than did the AG. After adjusting for confounders, the risk variables of BOP (P=0.047), smoking (P = 0.003), and diabetes (P = 0.028) for the PG and smoking (P=0.047) for the AG showed a negative influence on periodontal status. Conclusions: In both groups, the periodontal maintenance therapy minimized the negative effect of the risk variables. However, PG showed significantly less progression of periodontitis and tooth loss compared to AG. J Periodontol 2012;83:301-311. [Costa, Fernando Oliveira; Santuchi, Camila Carvalho; Pereira Lages, Eugenio Jose; Miranda Cota, Luis Otavio; Medeiros Lorentz, Telma Campos; Costa, Jose Eustaquio] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, Sheila Cavalca; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2011 Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig) (FAPEMIG-Brazil) [10137] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Background: To our knowledge, the association between personality traits and oral health impacts on daily activities among patients under periodontal maintenance was not previously evaluated. The present study aims to determine the oral health impacts among regular- and erratic-complier patients and their correlations with personality traits by means of the oral impacts on daily performance (OIDP) and the neuroticism extraversion openness five-factor inventory (NEO FFI-R) instruments. Methods: A total of 58 regular and 58 erratic compliers matched by sex, diabetes, and smoking were recruited from a cohort of 238 participants under periodontal maintenance. Periodontal examinations and questionnaire applications were performed in recalls during a 3-year interval. The influence of variables of interest on the OIDP was evaluated through univariate and multivariate linear regression analyses. Results: The periodontal status of regular compliers was significantly better than that of erratic compliers. Erratic compliers presented higher OIDP scores (36.34 +/- 6.64) compared to regular compliers (30.45 +/- 7.72). Higher scores of neuroticism and conscientiousness (R(2) = 68%; P

Recent studies suggest that stress can predispose an individual to the development of periodontal disease, but the exact biological mechanism is unknown. Considering that psychological stress can down-regulate the production of β-defensins (antimicrobial peptides produced in the oral cavity), the aim of the present study was to evaluate the association between stress and salivary levels of β-defensin 2 (HBD-2) and β-defensin 3 (HBD-3).For this purpose, seventy five volunteers, classified as periodontally healthy, were submitted to a psychological evaluation using a validated questionnaire (Questionnaire of Lipp-ISS). Following analysis of the questionnaires, the subjects were divided in two groups (Group A: Absence of stress and Group B: Presence of stress). Unstimulated saliva samples were collected and the concentration of total protein was determined using the BCA method, and the concentrations of HBD-2 and HBD-3 were determined by ELISA.The levels of total protein did not show a statistically significant difference between the groups. Analyses of HBD-2 and HBD-3 concentrations indicate that the stress condition was not associated with the levels of either peptide in saliva (P=0.3664 for HBD-2 and P=0.3608 for HBD-3).In periodontally healthy subjects, HBD-2 and HBD-3 levels are not influenced by stress.

To assess the prevalence and variables associated with gingival overgrowth (GO) in renal transplant recipients medicated with cyclosporine (CsA), tacrolimus (Tcr), or sirolimus (Sir).One hundred and thirty-five eligible subjects were divided in CsA, Tcr, and Sir groups comprising 45 subjects each. GO was visually assessed and subjects were assigned as GO+ or GO- in a post hoc definition. Saliva samples were collected and the presence of periodontal pathogens was assessed through polymerase chain reaction. Variables of interest were compared between GO+ and GO- subjects through univariate and multivariate analysis.Prevalence of GO was of 60.0% for CsA, 28.9% for Tcr, and 15.6% for Sir groups. Within the CsA group, GO was associated with papillary bleeding index (p=0.001); within the Tcr group, GO was associated with CsA previous use (p=0.013), and calcium channel blockers (CCB) use (p=0.003); within the Sir group, GO was associated with papillary bleeding index (p=0.018), and CCB use (p=0.020). A higher frequency of Tannerella forsythia was observed among GO+ subjects medicated with Tcr.Pharmacological and periodontal variables were associated with GO in different immunosuppressive regimens. Integration between the medical and the dental team may be an important approach in the post-transplant maintenance routine.

The presence of periodontal pathogens in the oral cavity may impact implant survival. Therefore, this study aimed to determine the prevalence of Campylobacter rectus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Eikenella corrodens, and Parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures.Thirty dentate subjects (mean age 61.7 +/- 7.05 years) and 30 edentulous subjects (mean age 65.8 +/- 8.05 years) were included in this cross-sectional study. Microbiological samples of cheek mucosa and the dorsum of the tongue were taken from all subjects. In addition, sulcus samples were taken from the dentate group. All samples were analysed using a bacterial DNA-specific polymerase chain reaction.All the pathogens studied were detected in dentate and edentulous subjects. When cheek and tongue samples were combined, C. rectus, A. actinomycetemcomitans and E. corrodens presented with a similar prevalence in both groups, whereas the other species were more prevalent specifically in the dentate group (P0.05). In dentate subjects, P. intermedia and T. denticola were present in higher frequencies in the cheek mucosa (26.67% and 66.67%, respectively), whereas P. gingivalis and T. forsythia were more prevalent in the tongue samples (26.67% and 56.67%, respectively).Periodontal pathogens may persist in the oral cavity of edentulous subjects who have had periodontal disease, even 1 year after the extraction of all teeth and in the absence of other hard surfaces in the mouth.

Made available in DSpace on 2019-09-12T16:26:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2010 Studies have suggested that A. actinomycetemcomitans is involved in the aetiology of aggressive periodontitis as well as in chronic periodontitis. This study was aimed at elucidating the occurrence of A. actinomycetemcomitans in a Brazilian population with chronic periodontitis. A total of 555 (mean age 33.04 ± 12.45) individuals, living in two large areas of the São Paulo State, namely "Baixada Santista" and "Vale do Paraíba", and diagnosed with mild [180 (mean age 29.59 ± 10.94)], moderate [241 (mean age 31.18 ± 11.45)] or severe [134 (mean age 33.04 ± 12.45)] chronic periodontitis were enrolled in this survey. Clinical exams including measurements of Probing Depth, Clinical Attachment Loss, Plaque and Gingival indices and subgingival microbiological assessments were performed at all population. The genomic DNA of A. actinomycetemcomitans was identified by Polymerase Chain Reaction from periodontal pocket samples. The occurrence of A. actinomycetemcomitans among chronic periodontitis subjects as well as its association with age and gender were statistically analysed using the Chi-square and Odds Ratio tests. The significance of differences was established at 5% (p < 0.05). A. actinomycetemcomitans was detected in 102 (18.37%) individuals: 29 (16.11%) mild; 42 (17.42%) moderate; and 31 (23.13%) severe chronic periodontitis with no statistical difference among groups. A higher occurrence of the searched bacterium was found both in the youngest group (p < 0.05) as well as in the female group (p < 0.05). This study elucidated that A. actinomycetemcomitans harbored subgingival pockets of our target group of chronic periodontitis subjects and that this bacterium seems to be inversely related to age, but related to the female gender. [Franco, Gilson Cesar Nobre] Universidade de Taubaté, Brazil Costa, Fernando Oliveira] Universidade Federal de Minas Gerais, Brazil Cortelli, José Roberto; Roman-Torres, Caio Vinícius Gonçalves; Aquino, Davi Romeiro; Cortelli, Sheila Cavalca] Universidade de Taubaté, Brazil

Made available in DSpace on 2019-09-12T16:53:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2010 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do estado de Minas Gerais (Fapemig) Objective: Interleukin-6 (IL-6) may be involved in drug-induced gingival overgrowth (GO). The present study was conducted to assess the association between IL-6 (-174 G/C) gene polymorphism and GO in renal transplant recipients under cyclosporine (CsA), tacrolimus (Tcr), or sirolimus (Sir)-based regimens. Methods: Within an eligible population, 45 unrelated subjects were selected for each CsA, Tcr, and Sir group, totaling a sample of 135 subjects. GO was visually assessed and subjects were assigned as controls (non-responders) or cases (responders) in a post hoc definition. IL-6 gene polymorphism was assessed using the polymerase chain reaction amplification and digestion. The distribution of genotypes and allele frequencies in responders and non-responders were compared using the Chi-squared test. Results: The number of responders was 27 (60.0%), 13 (28.9%), and 7 (15.6%) in the CsA, Tcr, and Sir groups, respectively. No differences could be observed at frequencies of -174GG, -174CG, and -174CC genotypes when comparing responders to non-responders in the CsA, Tcr, and Sir groups. Similar to genotypes, allele frequencies showed no differences between responders and non-responders in all groups. Conclusions: No association between IL-6 (-174 G/C) gene polymorphism and gingival overgrowth was observed in renal transplant recipients under CsA, Tcr, or Sir-based immunosuppressive maintenance regimens. (C) 2010 Elsevier Ltd. All rights reserved. [Miranda Cota, Luis Otavio; Viana, Michelle Beatriz; Moreira, Paula Rocha; Gomez, Ricardo Santiago; Costa, Fernando Oliveira] Univ Fed Minas Gerais, Dept Dent Clin Oral Pathol & Oral Surg, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazil [Cortelli, Jose Roberto; Cortelli, Sheila Cavalca] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:56:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2008 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universidade de Taubaté (Unitau) Background: The purpose of this study was to investigate the colonization of Campylobacter rectus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Prevotella intermedia, and Tannerella forsythia (previously T. forsythensis) in the tongue and cheek of newborns and elderly individuals with no teeth. Methods: Seventy-four edentulous subjects were included in this cross-sectional study. Microbiologic samples were taken from the dorsum of the tongue and cheek mucosa of all individuals and analyzed using a bacterial DNA-specific polymerase chain reaction. Results: C. rectus was the most prevalent species in both groups (20.9% in the cheek of newborns, and 77.4% in the tongue of elderly subjects). P. gingivalis and P. intermedia were not detected in any of the 43 newborns; however, P. gingivalis was recovered from the tongue and cheek (3.2%) of elderly individuals, whereas P. intermedia was detected in the tongue (9.6%) and cheek (3.2%) of elderly individuals. T. forsyth synthia was detected in newborns as well as elderly individuals, although the highest prevalence was observed in the tongue of newborns (6.9%) and elderly (9.6%) individuals. A. actinomycetemcomitans was not found in the tongue of newborns, but we observed A. actinomycetemcomitans in the cheek (2.3%) of newborns and in the tongue (12.9%) and cheek (6.4%) of elderly patients. Conclusions: Although we did not detect P. gingivalis and P. intermedia in newborns, periodontal pathogens could be detected from the oral mucous membranes of edentulous individuals. Our results suggest that major attention should be paid to edentulous individuals as an important measure in the prevention of the initial colonization of natural teeth and dental implants by periodontal pathogens. J Periodontol 2008; 79:7962-1965. [Cortelli, Jose Roberto; Aquino, Davi Romeiro; Cortelli, Sheila Cavalca; Nobre Franco, Gilson Cesar; Fernandes, Camila Borges; Goncalves Roman-Torres, Caio Vinicius] Universidade de Taubaté (Unitau), Dept Dent [Costa, Fernando Oliveira] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2008 This present study evaluated the salivary arginase activity (SAA) in patients with chronic periodontitis and the effect of periodontal therapy on the activity of such enzyme. Thirty-six patients (mean age, 45.97 +/- 14.52), 18 chronic periodontitis subjects (test group), and 18 periodontally healthy individuals (control group) participated in the study. Clinical periodontal examinations included measurements of probing pocket depth (PD), clinical attachment level (CAL), plaque (PI), and gingival (GI) indexes. The test group received periodontal therapy according to individual needs. The saliva sample was collected from all study population at baseline (both groups) and 30 days after periodontal therapy (test group). SAA was determined by measuring the L-ornithine formation from L-arginine and was expressed as mU/ml. The results showed that the mean values of SAA were statistically different between control and test groups. SAA was about 2.5 times higher in test than control groups. Thirty days after periodontal therapy, enzyme levels were 1.56 times lower than before periodontal therapy. We concluded that SAA is increased in chronic periodontitis subjects when compared to periodontally healthy individuals and that periodontal therapy significantly reduced SAA levels. It was' suggested that in the near future, SAA may be used as a salivary marker of periodontal status. [Gheren, L. W.; Hozhausen, M.] Universidade de Taubaté (Unitau), Dept Dent, BR-12010700 Taubate, SP, Brazil [Cortelli, J. R.] Universidade de Taubaté (Unitau), Dept Dent, Coordinat Periodont Res & Grad Studies Div [Rodrigues, E.] Universidade de Taubaté (Unitau), Dept Biol, Biochem Res & Grad Studies Div [Saad, W. A.] Universidade de Taubaté (Unitau), Physiol & Pharmacol Basic Inst Biosci