1. Tandem mass tag-based serum proteomic profiling revealed diabetic foot ulcer pathogenesis and potential therapeutic targets
- Author
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Xiao-Ting Yu, Feng Wang, Jia-Tong Ding, Bo Cai, Juan-Juan Xing, Guang-Hua Guo, and Fei Guo
- Subjects
Male ,Proteome ,Bioengineering ,General Medicine ,Middle Aged ,Applied Microbiology and Biotechnology ,Diabetic Foot ,proteomics ,Tandem Mass Spectrometry ,tmt ,Humans ,Female ,diabetic foot ulcer ,serum ,Biomarkers ,TP248.13-248.65 ,Aged ,Chromatography, Liquid ,Biotechnology - Abstract
Diabetic foot ulcer (DFU), one of the most serious complications of diabetes mellitus, is associated with a high amputation rate and decreased life quality. The impact of blood serum proteins on the occurrence and development of DFU has attracted a lot of interest. In this study, we aimed to define and compare the serum proteome of patients with DFU and healthy control (HC) to provide new insights into DFU pathogenesis. DFU patients and age- and sex-matched HCs were enrolled in this study (n = 54). We screened alterations in blood serum proteins from DFU patients and HC using a tandem mass tag (TMT) method based on liquid chromatography-mass spectrometry (LC-MS/MS) quantitative proteomics, and the differentially expressed proteins (DEPs) were further validated by parallel reaction monitoring (PRM) and enzyme-linked immunosorbent assay (ELISA). A total of 173 DEPs (100 up-regulated and 73 down-regulated) were identified between the DFU and HC groups (P
- Published
- 2022