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To observe the effect of acupuncture technique ofA total of 24 male SD rats were randomly divided into a sham operation group, a model group, a medication group and an acupuncture group after Morris water maze test, 6 rats in each group. VD model was established by permanent ligation of bilateral common carotid arteries in the model group, the medication group and the acupuncture group. Treatment was given on the next day after successful modeling. The rats in the acupuncture group were treated with acupuncture at "Baihui" (GV 20), "Shenting" (GV 24), "Shuigou" (GV 26), "Dazhui" (GV 14), "Fengfu" (GV 16), "Mingmen" (GV 4), "Neiguan" (PC 6), "Daling" (PC 7) and "Laogong" (PC 8) for 30 min; the rats in the medication group were treated with nimodipine solution (0.0625 g/kg) by gavage, once a day, for 2 weeks. Morris water maze test was used to detect the behavior of rats before modeling, 2 weeks after modeling and after intervention; after intervention, the expressions of VEGF and Ang-1 protein in hippocampus were detected by Western blot.Compared with the sham operation group, the average escape latency of rats in the model group was prolonged (The acupuncture technique of

To observe the effect of acupuncture on changes of neurological function and expression of proteins in the ischemic brain region in middle cerebral artery occlusion (MCAO) rats with protein chip technique, so as to reveal the profiles of cerebral proteins related to its effectiveness in improving cerebral ischemia (CI).SD rats were rando-mized into sham operation (sham), CI model, non-acupoint and acupoint groups (Compared with the sham group, the neurological deficit score was significantly higher in the model group (Manual acupuncture stimulation of GV 14, GV 20 and GV 26 can improve neurological function in CI rats, which may be associated with its function in regulating the expression of many proteins in the ischemic region of the brain.

To study the effect of electroacupuncture (EA) of Stomach Meridian acupoints on changes o of the related protein phosphorylation in the gastric mucosa involving the repair of the injured gastric mucosa in the rat.A total of 20 SD rats were divided into control, model, acupoint and non-acupoint groups, with 5 cases in each. Gastric mucosa injury model was established by intragastric perfusion of dehydrated alcohol (0.8 ml/100 g). EA (4 Hz/50 Hz, 1-3 mA) was applied to "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sibai" (ST 2), and the corresponding non-acupoints, once daily for 7 days. Gastric mucosal ulcer index (UI) was measured, and the profiling of the protein phosphorylation of interest in gastric mucosa cells was detected by antibody microarrays.In comparison with control group, the gastric mucosal UI of model group was significantly higher than that of control group (P0.01), while the UI of EA group was significant lower than those of model group and non-acupoint group (P0.01), suggesting a recovery of the injured mucous membrane. Findings of protein array analysis indicated that a total of 720 kinds of phosphorylated proteins (or = 1.5 folds) were found in the injured gastric mucosa. Compared to model group, the phosphorylation levels of 100 and 20 proteins were up-regulated, and 16 and 9 proteins down-regulated in acupoint and non-acupoint groups, respectively. The up-regulated proteins involve cell cycle mediation, cell proliferation, anti-apoptosis, and the regulation of the cell adhesion, cytoskeleton protein, inflammation, immune activities, etc. while the down-regulated proteins involve the down-regulation of the phosphorylation levels of the apoptosis proteins, adhesion proteins and cytoskeleton proteins, etc. In comparison to non-acupoint group, the phosphorylation levels of 100 proteins were up-regulated and 16 proteins down-regulated in acupoint group.EA of acupoints of the Stomach Meridian can promote repair of the injured gastric mucosa, which may be related to its effects in regulating the levels of phosphorylation of many signaling proteins.