1. CD19+IgD+CD27- Naïve B Cells as Predictors of Humoral Response to COVID 19 mRNA Vaccination in Immunocompromised Patients
- Author
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Eduard Schulz, Isabel Hodl, Patrick Forstner, Stefan Hatzl, Nazanin Sareban, Martina Moritz, Johannes Fessler, Barbara Dreo, Barbara Uhl, Claudia Url, Andrea J. Grisold, Michael Khalil, Barbara Kleinhappl, Christian Enzinger, Martin H. Stradner, Hildegard T. Greinix, Peter Schlenke, and Ivo Steinmetz
- Subjects
Adult ,Male ,Vaccines, Synthetic ,B cells ,COVID-19 Vaccines ,SARS-CoV-2 ,Immunology ,B-Lymphocyte Subsets ,COVID-19 ,Middle Aged ,RC581-607 ,Antibodies, Viral ,Antibodies, Neutralizing ,immunodeficiencies ,Immunocompromised Host ,Immunogenicity, Vaccine ,mRNA vaccine ,Immunology and Allergy ,Humans ,cancer ,Female ,mRNA Vaccines ,Immunologic diseases. Allergy ,Original Research ,Aged - Abstract
Immunocompromised patients are considered high-risk and prioritized for vaccination against COVID-19. We aimed to analyze B-cell subsets in these patients to identify potential predictors of humoral vaccination response. Patients (n=120) suffering from hematologic malignancies or other causes of immunodeficiency and healthy controls (n=79) received a full vaccination series with an mRNA vaccine. B-cell subsets were analyzed prior to vaccination. Two independent anti-SARS-CoV-2 immunoassays targeting the receptor-binding domain (RBD) or trimeric S protein (TSP) were performed three to four weeks after the second vaccination. Seroconversion occurred in 100% of healthy controls, in contrast to 67% (RBD) and 82% (TSP) of immunocompromised patients, while only 32% (RBD) and 22% (TSP) achieved antibody levels comparable to those of healthy controls. The number of circulating CD19+IgD+CD27- naïve B cells was strongly associated with antibody levels (ρ=0.761, P
- Published
- 2021