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Introduction: Contact lens wear is a major risk factor for Acanthamoeba keratitis. Disinfection solutions effective at killing Acanthamoeba organisms are important in preventing corneal infection. This study was therefore performed to gain insight into how commercial contact lens disinfecting solutions affect cysts of a clinical isolate of Acanthamoeba castellani. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Boston SIMPLICITY™, Opti-Free Express® and Complete™ were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 4 to 8 hours and later cultured onto non-nutrient agar Iawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Boston SIMPLICITY™, Opti-Free Express® and Complete™) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers but Boston SIMPLICITY™ was effective at inactivating the cysts at 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. Boston SIMPLICITY™ showed its effectiveness when the exposure time is extended to 8 hours. [ABSTRACT FROM AUTHOR]

Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in One™, Solo Care® Plus' and Renu Multi-Plus® were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in One™, Solo Care® Plus' and Renu Multi-Plus®) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers. [ABSTRACT FROM AUTHOR]

Summary: Cutaneous larva migrans is a common parasitic skin disease that can be easily prevented by wearing ‘protective’ footwear. However, this has been under-emphasized in terms of what constitutes the protective footwear. Even though the disease resolves spontaneously, the significant duration of the disease along with severity of pruritus make treatment unavoidable. Here, we present a very long-standing creeping eruption, which puzzled many attending clinicians handling the case, and the possibility of long socks as a causal effect on the development of cutaneous larva migrans infection. [Copyright &y& Elsevier]