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Start Over Descriptor "Blood smears" Topic malaria
9 results on '"Blood smears"'

1. Low Allelic Variation of Plasmodium falciparum msp-1 and msp-2 among Gold Miners in Central Kalimantan Province, Indonesia

Author

Heny Arwati, Trilianty Lestarisa, Indria Augustina, Etik Ainun Rohmah, Sri Subekti, Soedjajadi Keman, and Yoes Prijatna Dachlan

Subjects
Giemsa, Blood smears, Allelic variation, Malaria, Indonesia, Infectious and parasitic diseases, RC109-216
Abstract

Background: We aimed to find out the allelic variation of Pfmsp-1 and Pfmsp-2 among gold miners in Central Kalimantan Province, Indonesia using parasites’ DNA isolated from archived RDT and GSBS Methods: This study was done using the samples collected between 2017-2020 from health centers in Subdistrict of Mihing Raya, Danau Rawah, and Bukit Hindu as well as Kapuas District Health Laboratory in Central Kalimantan Province, Surabaya, Indonesia. Parasites DNA were isolated from RDT cartridges and GSBS of local and migrant gold miners. Species of Plasmodium were confirmed by single step PCR. The allelic variation of Pfmsp-1 (K1, MAD20, RO33) and Pfmsp-2 (3D7, FC27) were analyzed by nested PCR. Results: Pfmsp-1 gene was found in only two (22.22%) out of 9 local samples, and 3 (27.27%) out of 11 migrant samples were found positive for K1 (150 bp) as well as MAD 20 (190 bp) allelic families. Pfmsp-2 gene were found in each one sample of 550 bp fragment in local (11.11%) and migrant samples (9.09%) for 3D7, and 2 samples of 300 bp fragments in local (22.22%) and 3 samples of 300 bp in migrant samples (27.27%). No difference in size and number of infections between both populations. The RO33 allelic family Alhamdulillah was not found in any sample. Conclusion: Low allelic variation of Pfmsp-1 and Pfmsp-2 genes with monogenotype indicated the low intensity of malaria transmission among gold miners in the studied areas. Further, the transmission may occur locally in the mining sites.

Published
2023
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2. Low Allelic Variation of Plasmodium falciparum msp-1 and msp-2 among Gold Miners in Central Kalimantan Province, Indonesia.

Author

Arwati, Heny, Lestarisa, Trylianti, Augustina, Indria, Rohmah, Etik Ainun, Subekti, Sri, Keman, Soedjajadi, and Dachlan, Yoes Prijatna

Subjects
*GOLD miners, *PLASMODIUM falciparum, *PLASMODIUM, *PROVINCES, *MEDICAL centers, *DNA
Abstract

Background: We aimed to find out the allelic variation of Pfmsp-1 and Pfmsp-2 among gold miners in Central Kalimantan Province, Indonesia using parasites' DNA isolated from archived RDT and GSBS Methods: This study was done using the samples collected between 2017-2020 from health centers in Subdistrict of Mihing Raya, Danau Rawah, and Bukit Hindu as well as Kapuas District Health Laboratory in Central Kalimantan Province, Surabaya, Indonesia. Parasites DNA were isolated from RDT cartridges and GSBS of local and migrant gold miners. Species of Plasmodium were confirmed by single step PCR. The allelic variation of Pfmsp-1 (K1, MAD20, RO33) and Pfmsp-2 (3D7, FC27) were analyzed by nested PCR. Results: Pfmsp-1 gene was found in only two (22.22%) out of 9 local samples, and 3 (27.27%) out of 11 migrant samples were found positive for K1 (150 bp) as well as MAD 20 (190 bp) allelic families. Pfmsp-2 gene were found in each one sample of 550 bp fragment in local (11.11%) and migrant samples (9.09%) for 3D7, and 2 samples of 300 bp fragments in local (22.22%) and 3 samples of 300 bp in migrant samples (27.27%). No difference in size and number of infections between both populations. The RO33 allelic family Alhamdulillah was not found in any sample. Conclusion: Low allelic variation of Pfmsp-1 and Pfmsp-2 genes with monogenotype indicated the low intensity of malaria transmission among gold miners in the studied areas. Further, the transmission may occur locally in the mining sites. [ABSTRACT FROM AUTHOR]

Published
2023

3. Malaria Detection on Giemsa-Stained Blood Smears Using Deep Learning and Feature Extraction

Author

Dang, Nobel, Saraf, Varun, Khanna, Ashish, Gupta, Deepak, Sheikh, Tariq Hussain, Kacprzyk, Janusz, Series Editor, Pal, Nikhil R., Advisory Editor, Bello Perez, Rafael, Advisory Editor, Corchado, Emilio S., Advisory Editor, Hagras, Hani, Advisory Editor, Kóczy, László T., Advisory Editor, Kreinovich, Vladik, Advisory Editor, Lin, Chin-Teng, Advisory Editor, Lu, Jie, Advisory Editor, Melin, Patricia, Advisory Editor, Nedjah, Nadia, Advisory Editor, Nguyen, Ngoc Thanh, Advisory Editor, Wang, Jun, Advisory Editor, Khanna, Ashish, editor, Gupta, Deepak, editor, Bhattacharyya, Siddhartha, editor, Snasel, Vaclav, editor, Platos, Jan, editor, and Hassanien, Aboul Ella, editor

Published
2020
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4. A Survey on Peripheral Blood Smear Analysis Using Deep Learning

Author

Al-qudah, Rabiah, Suen, Ching Y., Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Lu, Yue, editor, Vincent, Nicole, editor, Yuen, Pong Chi, editor, Zheng, Wei-Shi, editor, Cheriet, Farida, editor, and Suen, Ching Y., editor

Published
2020
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5. Standardization of blood smears prepared in transparent acetate: an alternative method for the microscopic diagnosis of malaria.

Author

Mello, Marcia B. C., Luz, Francisco C., Leal-Santos, Fabio A., Alves Jr., Eduardo R., Gasquez, Thamires M., and Fontes, Cor J. F.

Subjects
*MALARIA diagnosis, *BLOOD platelets, *NEUTROPHILS, *STANDARDIZATION, *MICROSCOPY
Abstract

Background Due to students' initial inexperience, slides are frequently broken and blood smears are damaged in microscopy training, leading to the need for their constant replacement. To minimize this problem a method of preparing blood smears on transparent acetate sheets was developed with the goal of implementing appropriate and more readily available teaching resources for the microscopic diagnosis of malaria. Methods Acetate sheets derived from polyester were used to standardize the preparation and staining of thin and thick blood smears on transparent acetate sheets. Thick and thin blood smears were also prepared using the conventional method on glass slides. The staining was conducted using Giemsa staining for the thick and thin smears. Results Microscopic examination (1,000×) of the thin and thick blood smears prepared on transparent acetate produced high-quality images for both the parasites and the blood cells. The smears showed up on a clear background and with minimal dye precipitation. It was possible to clearly identify the main morphological characteristics of Plasmodium, neutrophils and platelets. After 12 months of storage, there was no change in image quality or evidence of fungal colonization. Conclusion Preparation of thin and thick blood smears in transparent acetate for the microscopic diagnosis of malaria does not compromise the morphological and staining characteristics of the parasites or blood cells. It is reasonable to predict the applicability of transparent acetate in relevant situations such as the training of qualified professionals for the microscopic diagnosis of malaria and the preparation of positive specimens for competency assessment (quality control) of professionals and services involved in the diagnosis of malaria. [ABSTRACT FROM AUTHOR]

Published
2014
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6. Hemoparásitos Presentes en Poblaciones Ferales de la Paloma de Castilla (Columba livia) en el Departamento de Lima, Perú

Author

Carlos Erazo, Nancy, Arellano Lima, Freddy, Puray Chavez, Nidia, Barraza Hernández, Adela, and Alcázar García, Paloma

Subjects
hemoparásitos, doves, frotis sanguíneo, malaria, hemoparasites, palomas, blood smears
Abstract

The aim of the study was to determine the presence of blood parasites in feral populations of the rock dove (Columba livia) in the department of Lima, Peru. The study was carried out in Pampas San Alejo, located in Barranca (rural area) and in a zoo located in San Juan de Miraflores district (urban area). A total of 52 adult birds were captured using mist nets, resulting 28 and 24 pigeons in the rural and urban area respectively. Blood samples from the brachial vein were collected, and blood smears were fixed with methanol and stained with Giemsa. Results showed 94.2% (49/52) positive to the presence of hemoparasites: Haemoproteus sp (94.2%; 47/52), Plasmodium sp (13.5%; 7/52) and Leucocytozoon sp (1.9%; 1/52). The Haemoproteus sp infestation intensity was very low (24.5%), low (59.2%) and medium (16.3%). All rural birds were positive, while only 87.5% (21/24) of birds in the urban area had hemoparasites, although this difference was not significant., El objetivo del presente estudio fue determinar la presencia de hemoparásitos en poblaciones ferales de la paloma de castilla (Columba livia) en el departamento de Lima, Perú. Se trabajó en el centro poblado Pampas San Alejo, ciudad y provincia de Barranca (zona rural) y en un Zoológico del distrito de San Juan de Miraflores, ciudad y provincia de Lima (zona urbana). Se capturaron 52 aves adultas utilizando redes de neblina: 28 y 24 aves en la zona rural y urbana, respectivamente. Se obtuvo una muestra sanguínea de la vena braquial y se realizaron frotices sanguíneos, que fueron fijados con metanol y teñidos con Giemsa. El 94.2% (49/52) de las aves fueron positivas a la presencia de hemoparásitos: Haemoproteus sp (94.2%; 47/52), Plasmodium sp (13.5%; 7/52) y Leucocytozoon sp (1.9%; 1/52). La intensidad de infección para Haemoproteus sp fue muy baja (24.5%), baja (59.2%) y media (16.3%). Todas las aves de la zona rural fueron positivas (n=28), mientras que solo el 87.5% (21/24) de las aves de la zona urbana presentaron hemoparásitos, pero sin diferencia significativa entre lugares de procedencia de las aves.

Published
2017

7. Hemoparásitos Presentes en Poblaciones Ferales de la Paloma de Castilla (Columba livia) en el Departamento de Lima, Perú

Author

Nidia Puray Chavez, Freddy Arellano Lima, Paloma Alcázar García, Nancy Carlos Erazo, and Adela Barraza Hernández

Subjects
Veterinary medicine, General Veterinary, frotis sanguíneo, malaria, Haemoproteus sp, medicine.disease_cause, Giemsa stain, hemoparásitos, Blood smear, Geography, doves, Infestation, Brachial vein, medicine, hemoparasites, palomas, blood smears, Plasmodium sp, Rural area, Blood parasites
Abstract

El objetivo del presente estudio fue determinar la presencia de hemoparásitos en poblaciones ferales de la paloma de castilla (Columba livia) en el departamento de Lima, Perú. Se trabajó en el centro poblado Pampas San Alejo, ciudad y provincia de Barranca (zona rural) y en un Zoológico del distrito de San Juan de Miraflores, ciudad y provincia de Lima (zona urbana). Se capturaron 52 aves adultas utilizando redes de neblina: 28 y 24 aves en la zona rural y urbana, respectivamente. Se obtuvo una muestra sanguínea de la vena braquial y se realizaron frotices sanguíneos, que fueron fijados con metanol y teñidos con Giemsa. El 94.2% (49/52) de las aves fueron positivas a la presencia de hemoparásitos: Haemoproteus sp (94.2%; 47/52), Plasmodium sp (13.5%; 7/52) y Leucocytozoon sp (1.9%; 1/52). La intensidad de infección para Haemoproteus sp fue muy baja (24.5%), baja (59.2%) y media (16.3%). Todas las aves de la zona rural fueron positivas (n=28), mientras que solo el 87.5% (21/24) de las aves de la zona urbana presentaron hemoparásitos, pero sin diferencia significativa entre lugares de procedencia de las aves. The aim of the study was to determine the presence of blood parasites in feral populations of the rock dove (Columba livia) in the department of Lima, Peru. The study was carriedout inPampasSanAlejo,locatedin Barranca(ruralarea) and ina zoo located in San Juan de Miraflores district (urban area). A total of 52 adult birds were captured using mist nets, resulting 28 and 24 pigeons in the rural and urban area respectively. Blood samples from the brachial vein were collected, and blood smears were fixed with methanol and stained with Giemsa. Results showed 94.2% (49/52) positive to the presence of hemoparasites: Haemoproteus sp (94.2%; 47/52), Plasmodium sp (13.5%; 7/52) and Leucocytozoon sp (1.9%; 1/52). The Haemoproteus sp infestation intensity was very low (24.5%), low (59.2%) and medium (16.3%).All rural birds were positive, while only 87.5% (21/24) of birds in the urban area had hemoparasites, although this difference was not significant

Published
2017
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9. Sex ratios in the rodent malaria parasite, Plasmodium chabaudi

Author

Sarah E. Reece, Stuart A. West, Alison B. Duncan, Andrew F. Read, and University of Edinburgh

Subjects
Male, law.invention, Plasmodium chabaudi, Mice, 0302 clinical medicine, Chloroquine, law, FERTILIZATION, FERTILITY INSURANCE, BERGHEI, blood smears, Sex allocation, 0303 health sciences, GAMETE DEVELOPMENT, 3. Good health, Infectious Diseases, Transmission (mechanics), Female, FALCIPARUM GAMETOCYTES, Sex ratio, medicine.drug, sex allocation theory, TRANSMISSION, 030231 tropical medicine, MOSQUITOS, Biology, Apicomplexa, Antimalarials, 03 medical and health sciences, parasitic diseases, medicine, Gametocyte, Animals, INFECTIVITY, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Sex Ratio, 030304 developmental biology, BLOOD PARASITES, biology.organism_classification, medicine.disease, mortality, Malaria, Mice, Inbred C57BL, Disease Models, Animal, gametocyte, Immunology, Animal Science and Zoology, Parasitology, COMMITMENT
Abstract

The sex ratios of malaria and related Apicomplexan parasites play a major role in transmission success. Here, we address 2 fundamental issues in the sex ratios of the rodent malaria parasite, Plasmodium chabaudi. First we test the accuracy of empirical methods for estimating sex ratios in malaria parasites, and show that sex ratios made with standard thin smears may overestimate the proportion of female gametocytes. Secondly, we test whether the mortality rate differs between male and female gametocytes, as assumed by sex ratio theory. Conventional application of sex ratio theory to malaria parasites assumes that the primary sex ratio can be accurately determined from mature gametocytes circulating in the peripheral circulation. We stopped gametocyte production with chloroquine in order to study a cohort of gametocytes in vitro. The mortality rate was significantly higher for female gametocytes, with an average half-life of 8 h for female gametocytes and 16 h for male gametocytes.

Published
2003
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