1. Elf4 regulates lysosomal biogenesis and the mTOR pathway to promote clearance of Staphylococcus aureus in macrophages
- Author
-
Yan He, Yunfan He, Tingyue Wu, Yanhua Kang, and Dongjiu Zhao
- Subjects
Staphylococcus aureus ,Biophysics ,medicine.disease_cause ,Biochemistry ,Microbiology ,03 medical and health sciences ,Phagocytosis ,Structural Biology ,In vivo ,Genetics ,medicine ,Humans ,Molecular Biology ,Transcription factor ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,0303 health sciences ,biology ,Macrophages ,TOR Serine-Threonine Kinases ,Intracellular parasite ,030302 biochemistry & molecular biology ,Cell Biology ,Staphylococcal Infections ,biology.organism_classification ,In vitro ,DNA-Binding Proteins ,Gene Expression Regulation ,Lysosomes ,Bacteria ,Biogenesis ,Transcription Factors - Abstract
Staphylococcus aureus is a major cause of infectious disease. Macrophages can directly destroy most of the invading bacteria through the phagolysosomal pathway. E74-like factor 4 (Elf4) is one of the important transcription factors that controls diverse pathogens, but the role of Elf4 in macrophage-mediated S. aureus eradication is unknown. Our data show that Elf4 is induced by S. aureus in macrophages. Elevated expression of Elf4 results in decreased bacterial load and inflammatory responses during S. aureus infection in vivo and in vitro. Elf4-overexpressed macrophages have decreased mTOR activity and increased lysosomal mass. Collectively, these results suggest that S. aureus induces Elf4 expression, which enhances lysosomal function and increases the capacity of macrophages to eliminate intracellular pathogens.
- Published
- 2021