1. The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages.
- Author
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Lail SS, McKenna N, and Yates RM
- Subjects
- Mice, Animals, Cell Differentiation genetics, Cell Line, Myeloid Progenitor Cells, Homeodomain Proteins genetics, Macrophages
- Abstract
The use of Hox-driven conditionally immortalized immune cells has significantly increased in biomedical research over the past 15 years. HoxB8-driven conditionally immortalized myeloid progenitor cells maintain their ability to differentiate into functional macrophages. There are multiple benefits to this conditional immortalization strategy including the ability for unlimited propagation, genetic mutability, primary-like immune cells (macrophages, dendritic cells, and granulocytes) on demand, derivation from variety of mouse strains, and simple cryopreservation and reconstitution. In this chapter, we will discuss how to derive and use these HoxB8-conditionally immortalized myeloid progenitor cells., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2023
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