20 results on '"Kubes, Paul"'
Search Results
2. NOX2: is the best defense a good offense?
- Author
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Zhang WX and Kubes P
- Subjects
- Animals, Homeostasis, Mice, Macrophages, NADPH Oxidases
- Published
- 2022
- Full Text
- View/download PDF
3. Programmed Death Ligand 1 Is Overexpressed in Liver Macrophages in Chronic Liver Diseases, and Its Blockade Improves the Antibacterial Activity Against Infections.
- Author
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Pose E, Coll M, Martínez-Sánchez C, Zeng Z, Surewaard BGJ, Català C, Velasco-de Andrés M, Lozano JJ, Ariño S, Fuster D, Niñerola-Bazán A, Graupera I, Muñoz É, Lozano F, Sancho-Bru P, Kubes P, and Ginès P
- Subjects
- Aged, Animals, Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic metabolism, B7-H1 Antigen antagonists & inhibitors, Bacterial Infections prevention & control, Biopsy, Cells, Cultured, Disease Models, Animal, Female, Gene Expression Profiling, Humans, Liver immunology, Liver pathology, Liver Cirrhosis complications, Liver Cirrhosis diagnosis, Liver Cirrhosis pathology, Macrophages metabolism, Male, Mice, Middle Aged, Phagocytosis, Primary Cell Culture, Receptors, Cell Surface metabolism, Receptors, Immunologic metabolism, Severity of Illness Index, B7-H1 Antigen metabolism, Bacterial Infections immunology, Immune Checkpoint Inhibitors administration & dosage, Liver Cirrhosis immunology, Macrophages immunology
- Abstract
Background and Aims: Bacterial infections are common and severe in cirrhosis, but their pathogenesis is poorly understood. Dysfunction of liver macrophages may play a role, but information about their function in cirrhosis is limited. Our aims were to investigate the specific profile and function of liver macrophages in cirrhosis and their contribution to infections. Macrophages from human cirrhotic livers were characterized phenotypically by transcriptome analysis and flow cytometry; function was assessed in vivo by single photon emission computerized tomography in patients with cirrhosis. Serum levels of specific proteins and expression in peripheral monocytes were determined by ELISA and flow cytometry. In vivo phagocytic activity of liver macrophages was measured by spinning disk intravital microscopy in a mouse model of chronic liver injury., Approach and Results: Liver macrophages from patients with cirrhosis overexpressed proteins related to immune exhaustion, such as programmed death ligand 1 (PD-L1), macrophage receptor with collagenous structure (MARCO), and CD163. In vivo phagocytic activity of liver macrophages in patients with cirrhosis was markedly impaired. Monocytes from patients with cirrhosis showed overexpression of PD-L1 that paralleled disease severity, correlated with its serum levels, and was associated with increased risk of infections. Blockade of PD-L1 with anti-PD-L1 antibody caused a shift in macrophage phenotype toward a less immunosuppressive profile, restored liver macrophage in vivo phagocytic activity, and reduced bacterial dissemination., Conclusion: Liver cirrhosis is characterized by a remarkable impairment of phagocytic function of macrophages associated with an immunosuppressive transcriptome profile. The programmed cell death receptor 1/PD-L1 axis plays a major role in the impaired activity of liver macrophages. PD-L1 blockade reverses the immune suppressive profile and increases antimicrobial activity of liver macrophages in cirrhosis., (© 2020 The Authors. Hepatology published by Wiley Periodicals LLC on behalf of American Association for the Study of Liver Diseases.)
- Published
- 2021
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- View/download PDF
4. Perivascular localization of macrophages in the intestinal mucosa is regulated by Nr4a1 and the microbiome.
- Author
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Honda M, Surewaard BGJ, Watanabe M, Hedrick CC, Lee WY, Brown K, McCoy KD, and Kubes P
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- Animals, CX3C Chemokine Receptor 1 metabolism, Colitis pathology, Dextran Sulfate, Dysbiosis pathology, Mice, Inbred C57BL, Monocytes metabolism, Receptors, CCR2 metabolism, Wound Healing, Gastrointestinal Microbiome, Intestinal Mucosa blood supply, Intestinal Mucosa cytology, Macrophages metabolism, Nuclear Receptor Subfamily 4, Group A, Member 1 metabolism
- Abstract
While the ontogeny and recruitment of the intestinal monocyte/macrophage lineage has been studied extensively, their precise localization and function has been overlooked. Here we show by imaging the murine small and large intestines in steady-state that intestinal CX3CR1
+ macrophages form an interdigitated network intimately adherent to the entire mucosal lamina propria vasculature. The macrophages form contacts with each other, which are disrupted in the absence of microbiome, monocyte recruitment (Ccr2-/- ), or monocyte conversion (Nr4a1-/- ). In dysbiosis, gaps exist between the perivascular macrophages correlating with increased bacterial translocation from the lamina propria into the bloodstream. The recruitment of monocytes and conversion to macrophages during intestinal injury is also dependent upon CCR2, Nr4a1 and the microbiome. These findings demonstrate a relationship between microbiome and the maturation of lamina propria perivascular macrophages into a tight anatomical barrier that might function to prevent bacterial translocation. These cells are also critical for emergency vascular repair.- Published
- 2020
- Full Text
- View/download PDF
5. Gata6 + Pericardial Cavity Macrophages Relocate to the Injured Heart and Prevent Cardiac Fibrosis.
- Author
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Deniset JF, Belke D, Lee WY, Jorch SK, Deppermann C, Hassanabad AF, Turnbull JD, Teng G, Rozich I, Hudspeth K, Kanno Y, Brooks SR, Hadjantonakis AK, O'Shea JJ, Weber GF, Fedak PWM, and Kubes P
- Subjects
- Animals, Cell Movement, Cells, Cultured, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Ventricular Remodeling, Fibrosis prevention & control, GATA6 Transcription Factor metabolism, Heart physiology, Macrophages immunology, Myocardial Infarction immunology, Myocardium pathology, Pericardium immunology
- Abstract
Macrophages play an important role in structural cardiac remodeling and the transition to heart failure following myocardial infarction (MI). Previous research has focused on the impact of blood-derived monocytes on cardiac repair. Here we examined the contribution of resident cavity macrophages located in the pericardial space adjacent to the site of injury. We found that disruption of the pericardial cavity accelerated maladaptive post-MI cardiac remodeling. Gata6
+ macrophages in mouse pericardial fluid contributed to the reparative immune response. Following experimental MI, these macrophages invaded the epicardium and lost Gata6 expression but continued to perform anti-fibrotic functions. Loss of this specialized macrophage population enhanced interstitial fibrosis after ischemic injury. Gata6+ macrophages were present in human pericardial fluid, supporting the notion that this reparative function is relevant in human disease. Our findings uncover an immune cardioprotective role for the pericardial tissue compartment and argue for the reevaluation of surgical procedures that remove the pericardium., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2019
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6. The surreptitious survival of the emerging pathogen Staphylococcus lugdunensis within macrophages as an immune evasion strategy.
- Author
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Flannagan RS, Watson DW, Surewaard BGJ, Kubes P, and Heinrichs DE
- Subjects
- Animals, Bacterial Toxins pharmacology, Cells, Cultured, Female, Humans, Kupffer Cells microbiology, Kupffer Cells pathology, Lysosomal Membrane Proteins metabolism, Macrophages drug effects, Macrophages immunology, Mice, Mice, Inbred BALB C, Peptidoglycan genetics, Peptidoglycan metabolism, Phagosomes microbiology, RAW 264.7 Cells, Staphylococcal Infections microbiology, Staphylococcal Infections pathology, Staphylococcus aureus chemistry, Staphylococcus aureus pathogenicity, Staphylococcus lugdunensis physiology, Host-Pathogen Interactions physiology, Immune Evasion, Macrophages microbiology, Staphylococcal Infections immunology, Staphylococcus lugdunensis pathogenicity
- Abstract
Staphylococcus lugdunensis is a commensal bacterium that can cause serious infection suggesting an ability to circumvent aspects of host immunity. We demonstrate here that macrophages fail to kill ingested S. lugdunensis and the bacteria persist for extended periods, without replicating, within mature LAMP-1-positive phagolysosomes. Phagocytosed S. lugdunensis also do not intoxicate host cells in contrast to Staphylococcus aureus. Optimal survival of S. lugdunensis requires O-acetylated peptidoglycan because an oatA mutant, which is more sensitive to killing by lysozyme than wild type, survived to a lesser extent in macrophages. In vitro models of macrophage infection reveal that viable intracellular S. lugdunensis bacteria can be made to grow by pharmacologic perturbation of phagosome function or by phagocyte intoxication by S. aureus toxins. Remarkably, replicating S. lugdunensis is not constrained by LAMP-1 and phosphatidylserine-positive endomembranes, which is distinct from S. aureus that replicates within phagolysosomes. In vivo, S. lugdunensis can also reside in the murine Kupffer cell where the bacteria persist without replicating and require oatA to resist killing in vivo. The intracellular environment of the macrophage represents a niche where S. lugdunensis can exist while protected from extracellular immune factors and may serve as a reservoir from which these bacteria could disseminate., (© 2018 John Wiley & Sons Ltd.)
- Published
- 2018
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7. Macrophages play an essential role in trauma-induced sterile inflammation and tissue repair.
- Author
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Peiseler M and Kubes P
- Subjects
- Animals, Humans, Immunity, Innate physiology, Inflammation physiopathology, Multiple Trauma physiopathology, Wound Healing physiology, Alarmins immunology, Inflammation immunology, Macrophages immunology, Multiple Trauma immunology
- Abstract
Severe trauma is accompanied by a profound activation of the immune system. Patients with polytrauma develop systemic inflammatory response syndrome (SIRS) and often sepsis, which contributes substantially to high mortality of this condition. On a cellular level, necrosis and loss of plasma membrane integrity lead to the release of endogenous "damage-associated molecular patterns" (DAMPs) as danger signals, which in turn activate innate immune cells. Inflammation that occurs in the absence of invading pathogens has been termed sterile inflammation and trauma with tissue damage represents an acute form of sterile inflammation. Macrophages are a heterogeneous group of phagocytes of the innate immune system and serve as sentinels to detect loss of tissue integrity. Macrophages show a remarkable plasticity and undergo phenotypical changes in response to injury and repair. Under basal conditions, tissue-resident macrophages are distributed in various organ systems and have critical functions in tissue development and the maintenance of homeostasis. Inflammatory conditions, such as major trauma, lead to the rapid recruitment of blood-derived monocytes that mature into macrophages as well as direct recruitment of macrophages from the cavity that surrounds the injured organ. This leads to augmentation of the pool of tissue-resident macrophages. Besides their essential role in sensing tissue damage and initiating inflammation, macrophages contribution critically to tissue repair and wound healing, ultimately allowing full restoration. Dysregulated sterile inflammation and defective healing result in chronic inflammatory disease with persistent tissue damage. In this review, we summarize the cellular and molecular mechanisms that lead to activation of sterile inflammation, recruitment of immune cells and initiation of wound healing. We focus on the pivotal role of macrophages played in this context.
- Published
- 2018
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8. Macrophages eliminate circulating tumor cells after monoclonal antibody therapy.
- Author
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Gül N, Babes L, Siegmund K, Korthouwer R, Bögels M, Braster R, Vidarsson G, ten Hagen TL, Kubes P, and van Egmond M
- Subjects
- Animals, Antibodies, Monoclonal chemistry, Bone Marrow Cells cytology, Cell Line, Tumor, Humans, Immunoglobulin G chemistry, Kupffer Cells cytology, Liver metabolism, Liver pathology, Melanoma, Experimental, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Microscopy, Fluorescence, Neoplasm Metastasis, Neoplasm Transplantation, Neoplasms immunology, Phagocytosis, Reactive Nitrogen Species, Reactive Oxygen Species, Antibodies, Monoclonal therapeutic use, Macrophages metabolism, Neoplastic Cells, Circulating metabolism
- Abstract
The use of monoclonal antibodies (mAbs) as therapeutic tools has increased dramatically in the last decade and is now one of the mainstream strategies to treat cancer. Nonetheless, it is still not completely understood how mAbs mediate tumor cell elimination or the effector cells that are involved. Using intravital microscopy, we found that antibody-dependent phagocytosis (ADPh) by macrophages is a prominent mechanism for removal of tumor cells from the circulation in a murine tumor cell opsonization model. Tumor cells were rapidly recognized and arrested by liver macrophages (Kupffer cells). In the absence of mAbs, Kupffer cells sampled tumor cells; however, this sampling was not sufficient for elimination. By contrast, antitumor mAb treatment resulted in rapid phagocytosis of tumor cells by Kupffer cells that was dependent on the high-affinity IgG-binding Fc receptor (FcγRI) and the low-affinity IgG-binding Fc receptor (FcγRIV). Uptake and intracellular degradation were independent of reactive oxygen or nitrogen species production. Importantly, ADPh prevented the development of liver metastases. Tumor cell capture and therapeutic efficacy were lost after Kupffer cell depletion. Our data indicate that macrophages play a prominent role in mAb-mediated eradication of tumor cells. These findings may help to optimize mAb therapeutic strategies for patients with cancer by helping us to aim to enhance macrophage recruitment and activity.
- Published
- 2014
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9. Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2.
- Author
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Audoy-Rémus J, Richard JF, Soulet D, Zhou H, Kubes P, and Vallières L
- Subjects
- Animals, Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic metabolism, Blood Vessels pathology, Cell Count, Cell Movement, Cell Proliferation, Cell Shape, Endotoxemia metabolism, Endotoxemia physiopathology, Green Fluorescent Proteins genetics, Interleukin-1beta deficiency, Interleukin-1beta metabolism, Mice, Mice, Inbred C57BL, Mice, Transgenic, Monocytes metabolism, Phenotype, Tumor Necrosis Factor-alpha deficiency, Tumor Necrosis Factor-alpha metabolism, Angiopoietin-2 metabolism, Brain blood supply, Cytokines metabolism, Endotoxemia pathology, Inflammation Mediators metabolism, Macrophages pathology, Monocytes pathology
- Abstract
The nervous system is constantly infiltrated by blood-derived sentinels known as perivascular macrophages. Their immediate precursors have not yet been identified in situ and the mechanism that governs their recruitment is mostly unknown. Here, we provide evidence that CD68(+)GR1(-) monocytes can give rise to perivascular macrophages in mice suffering from endotoxemia. After adhesion to the endothelium, these monocytes start to crawl, adopt a rod-shaped morphology when passing through capillaries, and can manifest the ability to proliferate and form a long cytoplasmic protuberance. They are attracted in greater numbers during endotoxemia by a combination of vasoregulatory molecules, including TNF (tumor necrosis factor), interleukin-1beta, and angiopoietin-2. After a period of several hours, some of them cross the endothelium to expand the population of perivascular macrophages. Depletion of adherent monocytes and perivascular macrophages can be achieved by injection of anti-angiopoietin-2 peptide-Fc fusion protein. This study extends our understanding of the behavior of monocytes at the blood-brain interface and provides a way to block their infiltration into the nervous tissue under inflammatory conditions.
- Published
- 2008
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10. Peritoneal [GATA6.sup.+] macrophages function as a portal for Staphylococcus aureus dissemination
- Author
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Jorch, Selina K., Surewaard, Bas G.J., Hossain, Mokarram, Peiseler, Moritz, Deppermann, Carsten, Deng, Jennifer, Bogoslowski, Ania, van der Wal, Fardau, Omri, Abdelwahab, Hickey, Michael J., and Kubes, Paul
- Subjects
Thermo Fisher Scientific Inc. ,Staphylococcus aureus infections ,Bacteria ,Macrophages ,Staphylococcus aureus ,Liver ,Kidney diseases ,Methicillin ,Scientific equipment industry ,Antibacterial agents ,Vancomycin ,Peritonitis ,Health care industry - Abstract
Essentially all Staphylococcus aureus (S. aureus) bacteria that gain access to the circulation are plucked out of the bloodstream by the intravascular macrophages of the liver--the Kupffer cells. It is also thought that these bacteria are disseminated via the bloodstream to other organs. Our data show that S. aureus inside Kupffer cells grew and escaped across the mesothelium into the peritoneal cavity and immediately infected GATA-binding factor 6-positive (GATA6+) peritoneal cavity macrophages. These macrophages provided a haven for S. aureus, thereby delaying the neutrophilic response in the peritoneum by 48 hours and allowing dissemination to various peritoneal and retroperitoneal organs including the kidneys. In mice deficient in GATA6+ peritoneal macrophages, neutrophils infiltrated more robustly and reduced S. aureus dissemination. Antibiotics administered i.v. did not prevent dissemination into the peritoneum or to the kidneys, whereas peritoneal administration of vancomycin (particularly liposomal vancomycin with optimized intracellular penetrance capacity) reduced kidney infection and mortality, even when administered 24 hours after infection. These data indicate that GATA6+ macrophages within the peritoneal cavity are a conduit of dissemination for i.v. S. aureus, and changing the route of antibiotic delivery could provide a more effective treatment for patients with peritonitis- associated bacterial sepsis., Introduction In humans, Staphylococcus aureus (S. aureus) bacteremia is the most common serious bacterial blood infection globally. In the United Kingdom alone, approximately 12,500 cases each year are reported with [...]
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- 2019
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11. Imaging reveals novel innate immune responses in lung, liver, and beyond*.
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Neupane, Arpan Sharma and Kubes, Paul
- Subjects
- *
IMMUNE response , *LIVER , *LUNGS , *LEUCOCYTES - Abstract
Highly dynamic immune responses are generated toward pathogens or injuries, in vivo. Multiple immune cell types participate in various facets of the response which leads to a concerted effort in the removal and clearance of pathogens or injured tissue and a return to homeostasis. Intravital microscopy (IVM) has been extensively utilized to unravel the dynamics of immune responses, visualizing immune cell behavior in intact living tissues, within a living organism. For instance, the phenomenon of leukocyte recruitment cascade. Importantly, IVM has led to a deep appreciation that immune cell behavior and responses in individual organs are distinct, but also can influence one another. In this review, we discuss how IVM as a tool has been used to study the innate immune responses in various tissues during homeostasis, injury, and infection. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
12. Innate immune cells orchestrate the repair of sterile injury in the liver and beyond.
- Author
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Hossain, Mokarram and Kubes, Paul
- Subjects
NATURAL immunity ,INFLAMMATION treatment ,LIVER injuries ,NEUTROPHILS ,MONOCYTES ,MACROPHAGES - Abstract
There is a close association between inflammation and sterile injury, however not all sterile injuries are the same. While a regulated inflammatory response is crucial for proper healing, a dysregulated or nonterminating response leads to disrepair. While immune cells are thought to contribute to the disrepair, they may also be critical for proper healing and as such, their actions may dictate the end result. In all forms of sterile injury, release of damage‐associated molecular patterns from necrotic cells causes robust recruitment of innate immune cells. The subsequent release of toxic mediators from immune cells is thought to be damaging in non‐resolving sterile injuries in which the dysregulated immune response leads to chronic inflammatory disease. While similar mediators may be released from immune cells in resolution of acute injury, the spatial localization, timing, and self‐termination may all be critical. In this review, we summarize the recent advances in our understanding of the temporal and spatial recruitment of various innate immune cells that beget appropriate healing of acute injuries. Where possible we try to compare this appropriate response to dysregulated sterile injuries in an attempt to identify novel therapeutic targets. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
13. Start a fire, kill the bug: The role of platelets in inflammation and infection.
- Author
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Deppermann, Carsten and Kubes, Paul
- Subjects
- *
BLOOD platelets , *KUPFFER cells , *INFECTION , *MACROPHAGES , *NEUTROPHILS , *THROMBOSIS , *HEMOSTASIS - Abstract
Platelets are the main players in thrombosis and hemostasis; however they also play important roles during inflammation and infection. Through their surface receptors, platelets can directly interact with pathogens and immune cells. Platelets form complexes with neutrophils to modulate their capacities to produce reactive oxygen species or form neutrophil extracellular traps. Furthermore, they release microbicidal factors and cytokines that kill pathogens and influence the immune response, respectively. Platelets also maintain the vascular integrity during inflammation by a mechanism that is different from classical platelet activation. In this review we summarize the current knowledge about how platelets interact with the innate immune system during inflammation and infection and highlight recent advances in the field. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
14. A Reservoir of Mature Cavity Macrophages that Can Rapidly Invade Visceral Organs to Affect Tissue Repair.
- Author
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Wang, Jing and Kubes, Paul
- Subjects
- *
MACROPHAGES , *INFLAMMATION , *VISCERA , *LEUCOCYTES , *TISSUE wounds , *RESERVOIRS , *LIVER injuries , *THERAPEUTICS - Abstract
Summary A key feature of inflammation is the timely recruitment of leukocytes, including monocytes, from blood into tissues, the latter maturing into macrophages over a period of 2–3 days. Using multi-channel spinning disk microscopy, we identified a rapid pathway of macrophage recruitment into an injured organ via a non-vascular route requiring no maturation from monocytes. In response to a sterile injury in liver, a reservoir of fully mature F4/80 hi GATA6 + peritoneal cavity macrophages rapidly invaded into afflicted tissue via direct recruitment across the mesothelium. The invasion was dependent on CD44 and DAMP molecule ATP and resulted in rapid replication and switching of macrophage toward an alternatively activated phenotype. These macrophages dismantled the nuclei of necrotic cells releasing DNA and forming a cover across the injury site. Rapid invasion of mature macrophages from body cavity with capacity for induction of reparative phenotype may impact altered tissues ranging from trauma to infections to cancer. Video Abstract [ABSTRACT FROM AUTHOR]
- Published
- 2016
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- View/download PDF
15. Lipoteichoic Acid Induces Unique Inflammatory Responses when Compared to Other Toll-Like Receptor 2 Ligands.
- Author
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Long, Elizabeth M., Millen, Brandie, Kubes, Paul, and Robbins, Stephen M.
- Subjects
RECEPTOR-ligand complexes ,COMPLEX compounds ,LIGAND binding (Biochemistry) ,MACROPHAGES ,GENE expression ,MICE ,ANIMAL models in research - Abstract
Toll-like receptors (TLRs) recognize evolutionarily-conserved molecular patterns originating from invading microbes. In this study, we were interested in determining if microbial ligands, which use distinct TLR2-containing receptor complexes, represent unique signals to the cell and can thereby stimulate unique cellular responses. Using the TLR2 ligands, R-FSL1, SFSL1, Pam2CSK4, Pam3CSK4, and lipoteichoic acid (LTA), we demonstrate that these ligands activate NF-kB and MAP Kinase pathways with ligand-specific differential kinetics in murine macrophages. Most strikingly, LTA stimulation of these pathways was substantially delayed when compared with the other TLR2 ligands. These kinetics differences were associated with a delay in the LTA-induced expression of a subset of genes as compared with another TLR2 ligand, R-FSL1. However, this did not translate to overall differences in gene expression patterns four hours following stimulation with different TLR2 ligands. We extended this study to evaluate the in vivo responses to distinct TLR2 ligands using a murine model of acute inflammation, which employs intravital microscopy to monitor leukocyte recruitment into the cremaster muscle. We found that, although R-FSL1, S-FSL1, Pam2CSK4, and Pam3CSK4 were all able to stimulate robust leukocyte recruitment in vivo, LTA remained functionally inert in this in vivo model. Therefore distinct TLR2 ligands elicit unique cellular responses, as evidenced by differences in the kinetic profiles of signaling and gene expression responses in vitro, as well as the physiologically relevant differences in the in vivo responses to these ligands. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
16. Cellular and molecular mechanisms underlying LPS-associated myocyte impairment.
- Author
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Tavener, Samantha A. and Kubes, Paul
- Subjects
- *
IMMUNOSUPPRESSION , *IMMUNOREGULATION , *IMMUNODEFICIENCY , *MUSCLE cells , *NEUTROPHILS , *GRANULOCYTES - Abstract
Recently we reported that Toll-like receptor 4 (TLR4)-positive immune cells of unknown identity were responsible for the LPS-induced depression of cardiac myocyte shortening. The aim of this study is to identify the TLR4-positive cell type that is responsible for the LPS-induced cardiac dysfunction. Neither neutrophil depletion alone nor mast cell deficiency had any impact on the impairment of myocyte shortening during LPS treatment. In contrast, LPS-treated, macrophage-deficient mice demonstrated a partial reduction in shortening compared with saline-treated, macrophage-deficient mice. Because the removal of macrophages could only partially restore myocyte shortening, we also investigated the effects of removing both neutrophils and macrophages on myocyte shortening. Interestingly, endotoxemic, neutrophil-depleted, and macrophage-deficient mice had completely restored myocyte shortening. Because both macrophages and neutrophils can produce nitric oxide (NO) and TNF-α, we examined LPS-treated inducible NO synthase knockout (iNOSKO) mice and TNF receptor (TNFR)-deficient mice. Eliminating both TNFR1 and TNFR2 was required to restore myocyte shortening during LPS treatment, whereas iNOS deficiency had no effect. These data suggest that macrophages and to a lesser degree neutrophils cause cardiac impairment, presumably via TNF-α. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
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17. The versatile platelet contributes to inflammation, infection, hemostasis, coagulation and cancer.
- Author
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Kubes, Paul
- Subjects
- *
BLOOD platelets , *INFLAMMATION , *MACROPHAGES , *LIVER cells , *IMMUNE response - Published
- 2016
- Full Text
- View/download PDF
18. Gata6+ large peritoneal macrophages: an evolutionarily conserved sentinel and effector system for infection and injury.
- Author
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Salm, Lilian, Shim, Raymond, Noskovicova, Nina, and Kubes, Paul
- Subjects
- *
PERITONEAL macrophages , *SEA urchins , *PHAGOCYTES , *MACROPHAGES , *TRANSCRIPTION factors - Abstract
Protective features of mammalian cavity macrophages are highly conserved from over 500 million years of evolution, with strong similarities found in sea urchin coelomocytes. The macrophage disappearance reaction (MDR) does not reflect the various events that occur during the perturbation of the peritoneum; we propose that the term 'macrophage disturbance of homeostasis reaction' (MDHR) would better reflect this process going forward. Crucial roles of murine cavity macrophages, such as Gata6+ large peritoneal macrophages, have been identified for injury repair upon breaching the mesothelium of visceral organs. The bona fide expression of GATA6 on human cavity macrophages might depend on donor or patient status upon sample collection. Mammalian cavity macrophages may have detrimental roles in diseases, such as cancer, as well as endometriosis, and postsurgical adhesions. Macrophages reside in the cavities of multicellular lifeforms, ranging from sea urchins to mice to humans. Such macrophages, expressing transcription factor Gata6 in mice, and potentially GATA6 in humans, are efficient, evolutionarily conserved sentinels, effectively detecting and responding to infection and cavity breaches. Gata6+ large peritoneal macrophages not only maintain cavity homeostasis, but can also contribute to diseases, such as cancer, postsurgical adhesions, and endometriosis. A deeper understanding of these mechanisms has strong potential for developing new candidate treatment strategies. There are striking similarities between the sea urchin cavity macrophage-like phagocytes (coelomocytes) and mammalian cavity macrophages in not only their location, but also their behaviors. These cells are crucial for maintaining homeostasis within the cavity following a breach, filling the gap and functioning as a barrier between vital organs and the environment. In this review, we summarize the evolving literature regarding these Gata6+ large peritoneal macrophages (GLPMs), focusing on ontogeny, their responses to perturbations, including their rapid aggregation via coagulation, as well as scavenger receptor cysteine-rich domains and their potential roles in diseases, such as cancer. We challenge the 50-year old phenomenon of the 'macrophage disappearance reaction' (MDR) and propose the new term 'macrophage disturbance of homeostasis reaction' (MDHR), which may better describe this complex phenomenon. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
19. Immune mechanisms linking metabolic injury to inflammation and fibrosis in fatty liver disease – novel insights into cellular communication circuits.
- Author
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Peiseler, Moritz, Schwabe, Robert, Hampe, Jochen, Kubes, Paul, Heikenwälder, Mathias, and Tacke, Frank
- Subjects
- *
FATTY liver , *HEPATIC fibrosis , *NUTRITIONAL genomics , *NON-alcoholic fatty liver disease , *THERAPEUTICS , *INFLAMMATION , *LIVER cells - Abstract
Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease and is emerging as the leading cause of cirrhosis, liver transplantation and hepatocellular carcinoma (HCC). NAFLD is a metabolic disease that is considered the hepatic manifestation of the metabolic syndrome; however, during the evolution of NAFLD from steatosis to non-alcoholic steatohepatitis (NASH), to more advanced stages of NASH with liver fibrosis, the immune system plays an integral role. Triggers for inflammation are rooted in hepatic (lipid overload, lipotoxicity, oxidative stress) and extrahepatic (gut-liver axis, adipose tissue, skeletal muscle) systems, resulting in unique immune-mediated pathomechanisms in NAFLD. In recent years, the implementation of single-cell RNA-sequencing and high dimensional multi-omics (proteogenomics, lipidomics) and spatial transcriptomics have tremendously advanced our understanding of the complex heterogeneity of various liver immune cell subsets in health and disease. In NAFLD, several emerging inflammatory mechanisms have been uncovered, including profound macrophage heterogeneity, auto-aggressive T cells, the role of unconventional T cells and platelet-immune cell interactions, potentially yielding novel therapeutics. In this review, we will highlight the recent discoveries related to inflammation in NAFLD, discuss the role of immune cell subsets during the different stages of the disease (including disease regression) and integrate the multiple systems driving inflammation. We propose a refined concept by which the immune system contributes to all stages of NAFLD and discuss open scientific questions arising from this paradigm shift that need to be unravelled in the coming years. Finally, we discuss novel therapeutic approaches to target the multiple triggers of inflammation, including combination therapy via nuclear receptors (FXR agonists, PPAR agonists). [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
20. Mice that exclusively express TLR4 on endothelial cells can efficiently clear a lethal systemic Gram-negative bacterial infection.
- Author
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Andonegui, Graciela, Hong Zhou, Bullard, Daniel, Kelly, Margaret M., Mullaly, Sarah C., McDonald, Braedon, Long, Elizabeth M., Robbins, Stephen M., Kubes, Paul, and Zhou, Hong
- Subjects
- *
VASCULAR endothelial growth factors , *MACROPHAGES , *GRAM-negative bacteria , *ENDOTHELIUM , *ESCHERICHIA coli , *GRAM-negative bacterial diseases , *EPITHELIAL cells , *CELL receptors , *ANIMAL experimentation , *CELL motility , *COMPARATIVE studies , *CYTOKINES , *RESEARCH methodology , *MEDICAL cooperation , *MICE , *MICROCIRCULATION , *NEUTROPHILS , *RESEARCH , *RESEARCH funding , *EVALUATION research , *LIPOPOLYSACCHARIDES , *PHYSIOLOGY , *CELL physiology - Abstract
Recognition of LPS by TLR4 on immune sentinel cells such as macrophages is thought to be key to the recruitment of neutrophils to sites of infection with Gram-negative bacteria. To explore whether endothelial TLR4 plays a role in this process, we engineered and imaged mice that expressed TLR4 exclusively on endothelium (known herein as EndotheliumTLR4 mice). Local administration of LPS into tissue induced comparable neutrophil recruitment in EndotheliumTLR4 and wild-type mice. Following systemic LPS or intraperitoneal E. coli administration, most neutrophils were sequestered in the lungs of wild-type mice and did not accumulate at primary sites of infection. In contrast, EndotheliumTLR4 mice showed reduced pulmonary capillary neutrophil sequestration over the first 24 hours; as a result, they mobilized neutrophils to primary sites of infection, cleared bacteria, and resisted a dose of E. coli that killed 50% of wild-type mice in the first 48 hours. In fact, the only defect we detected in EndotheliumTLR4 mice was a failure to accumulate neutrophils in the lungs following intratracheal administration of LPS; this response required TLR4 on bone marrow-derived immune cells. Therefore, endothelial TLR4 functions as the primary intravascular sentinel system for detection of bacteria, whereas bone marrow-derived immune cells are critical for pathogen detection at barrier sites. Nonendothelial TLR4 contributes to failure to accumulate neutrophils at primary infection sites in a disseminated systemic infection. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
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