1. GM-CSF and IL-4 produced by NKT cells inversely regulate IL-1β production by macrophages.
- Author
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Ahn S, Jeong D, Oh SJ, Ahn J, Lee SH, and Chung DH
- Subjects
- Animals, Caspase 1 metabolism, Cytokines metabolism, Inflammasomes metabolism, Lipopolysaccharides immunology, Mice, Mice, Knockout, NLR Family, Pyrin Domain-Containing 3 Protein metabolism, Granulocyte-Macrophage Colony-Stimulating Factor metabolism, Interleukin-1beta metabolism, Interleukin-4 metabolism, Macrophages immunology, Macrophages metabolism, Natural Killer T-Cells immunology, Natural Killer T-Cells metabolism
- Abstract
Natural Killer T (NKT) cells are distinct T cell subset that link innate and adaptive immune responses. IL-1β, produced by various immune cells, plays a key role in the regulation of innate immunity in vivo. However, it is unclear whether NKT cells regulate IL-1β production by macrophages. To address this, we co-cultured NKT cells and peritoneal macrophages in the presence of TCR stimulation and inflammasome activators. Among cytokines secreted from NKT cells, GM-CSF enhanced IL-1β production by macrophages via regulating LPS-mediated pro-IL-1β expression and NLRP3-dependent inflammasome activation, whereas IL-4 enhanced M2-differentiation of macrophages and decreased IL-1β production. Together, our findings suggest the NKT cells have double-sided effects on IL-1β-mediated innate immune responses by producing IL-4 and GM-CSF. These findings may be helpful for a comprehensive understanding of NKT cell-mediated regulatory mechanisms of the pro-inflammatory effects of IL-1β in inflammatory diseases in vivo., (Copyright © 2017 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2017
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