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In this study, a system for measuring the positional accuracy of industrial robots using 3D vision and networking technology has been proposed. The 3D vision system comprises a charge-coupled device (CCD) module, a reference sphere module, and a signal processing module. The reference sphere module serves as the sensing object, while the CCD module captures displacement images of the reference sphere module. The signal processing module receives and processes signals from the CCD module. It utilizes a self-developed Visual C + + interface to calculate the X, Y, Z, θx, and θy (five-degree-of-freedom, 5-DOF) positional accuracy of the industrial robot. Experimental results demonstrate that the linear accuracy (X, Y, Z) and angular accuracy (θx, θy) of the 3D vision system are approximately 3 μm and 0.15°, respectively. Additionally, a networking architecture connects the controller of the machine tool with the industrial robot to enable synchronized motion path planning. The industrial robot can be calibrated using the high-precision machine tool equipped with the 3D vision-based positional accuracy measurement system. [ABSTRACT FROM AUTHOR]