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8 results on '"Kay HD"'

1. A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity.

Author

Kay HD, Bonnard GD, West WH, and Herberman RB

Subjects
Ammonium Chloride pharmacology, Antigen-Antibody Reactions, Bacterial Proteins pharmacology, Cell Separation, Cytotoxicity Tests, Immunologic, Humans, Immunologic Techniques, Staphylococcus aureus immunology, Trypsin pharmacology, Antibody Specificity, Binding Sites, Antibody, Immunity, Cellular, Immunoglobulin Fc Fragments, Lymphocytes immunology
Published
1977

2. A comparative study on in vitro cytotoxic reactions of lymphocytes from normal donors and patients with sarcomas to cultured tumor cells.

Author

Kay HD, Thota H, and Sinkovics JG

Subjects
Adolescent, Adult, Aged, Animals, Cell Line, Cell Separation, Cells, Cultured, Cytotoxicity Tests, Immunologic, Female, Humans, Immunity, Cellular, Male, Middle Aged, Carcinoma immunology, Lymphocytes immunology, Sarcoma immunology, Sarcoma, Experimental immunology
Published
1976
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4. Letter: Cytotoxic lymphocytes from normal donors.

Author

Kay HD and Sinkovics JG

Subjects
Blood Donors, Cell Line, Cells, Cultured, Epitopes, Humans, Carcinoma, Transitional Cell immunology, Cytotoxicity Tests, Immunologic, Lymphocytes immunology, Sarcoma immunology
Published
1974

5. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells.

Author

West WH, Cannon GB, Kay HD, Bonnard GD, and Herberman RB

Subjects
Antigen-Antibody Complex, Binding Sites, Cell Line, Complement System Proteins, Cytotoxicity Tests, Immunologic, Humans, Immune Adherence Reaction, Immunoglobulin Fc Fragments, Immunoglobulin G, Macrophages immunology, T-Lymphocytes immunology, Bone Marrow immunology, Bone Marrow Cells, Immunity, Cellular, Leukemia, Myeloid immunology, Lymphocytes immunology
Abstract

Using a series of techniques to identify and deplete various peripheral blood lymphocyte subpopulations, we studied the cytotoxic reactivity of normal individuals against the myeloid cell line K-562 in a 4-hr 51chromium-release assay. Depletion of lymphocytes bearing complement receptors had a variable, usually negligible effect on cytotoxicity. In contrast, depletion of lymphocytes bearing Fc receptors abrogated target cell lysis. Separation of lymphocytes with high-affinity binding of sheep red blood cells (SRBC) evidenced by rosette formation at 29 degrees C yielded a population of rosette-forming cells containing few cytotoxic cells, whereas separation of total E-RFC under optimal rosetting conditions produced a rosette fraction containing a major proportion of the effector cells. These data indicate that the cytotoxic lymphocyte in this system is Fc receptor positive, largely complement receptor negative, and may possess low density or low affinity receptors for SRBC.

Published
1977

6. A simplified method for isolating bovine lymphocytes.

Author

Kay HD and Kaeberle ML

Subjects
Animals, Cattle, Cell Count, Cell Survival, Centrifugation, Erythrocytes, Gossypium, Leukocytes, Methods, Syringes, Histological Techniques, Lymphocytes cytology
Published
1972

7. The concept of immunotherapy of human sarcomas: the need for an in vitro monitoring assay.

Author

Sinkovics JG, Campos LT, Kay HD, Loh KK, Cabiness JR, and Ervin F

Subjects
Adolescent, Adult, Animals, Antigens, Neoplasm, Cattle, Cell Line, Cells, Cultured, Cross Reactions, Female, Humans, Immunity, Cellular, In Vitro Techniques, Male, Middle Aged, Sarcoma therapy, Cytotoxicity Tests, Immunologic, Immunotherapy, Lymphocytes immunology, Sarcoma immunology
Published
1973

8. Vaccination of the African white-tailed rat, Mystromys albacaudatus, with sonicated Leishmania braziliensis panamensis promastigotes

Author

Romito R, Beacham Be, and Kay Hd

Subjects
Male, Lymphocyte Activation, Sonication, Immune system, Antigen, Virology, medicine, Animals, Lymphocytes, Leishmaniasis, Antiserum, Leishmania, biology, Immune Sera, Vaccination, medicine.disease, biology.organism_classification, Leishmania braziliensis, Muridae, Infectious Diseases, Immunization, Parasitology, Female
Abstract

The usefulness of sonicated Leishmania braziliensis panamensis promastigotes for vaccination was evaluated in Mystromys albacaudatus, the African white-tailed rat. Thirty-two animals received three intradermal injections of 2 x 10(6) sonicated promastigotes derived from L. braziliensis panamensis at weekly intervals. One month after completion of the immunization schedule, the experimental group of animals was challenged in vivo with 2 x 10(6) live L. braziliensis panamensis promastogotes. At the same time, a matched group of 40 control animals was similarly challenged. Within 2 months, 35 of the 40 animals (87.5%) the control group developed ulcers, while only 14 of 32 previously vaccinated animals (43.7%) developed ulcers at the site of challenge within the same time period. The remaining 18 vaccinated animals (56.2%) remain free from ulcers 7 months after challenge. When lymphocytes from the spleens of vaccinated and control animals were challenged in vitro with antigen derived from sonicates of varying numbers of promastigotes, only cells from immunized animals responded vigorously to the antigenic challenge, a response which was not enhanced by the addition of immune antiserum to the reaction.

Published
1982

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