27 results on '"Grossi C"'
Search Results
2. Emergence of a B-cell lymphoblastic lymphoma in a patient with B-cell chronic lymphocytic leukemia: evidence for the single-cell origin of the two tumors.
- Author
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Pistoia V, Roncella S, Di Celle PF, Sessarego M, Cutrona G, Cerruti G, Boccaccio GP, Grossi CE, Foà R, and Ferrarini M
- Subjects
- Adult, Aged, Antibodies, Monoclonal, Antigens, Surface analysis, Burkitt Lymphoma blood, Burkitt Lymphoma genetics, Burkitt Lymphoma immunology, Cell Line, Cells, Cultured, Chromosome Aberrations, Chromosome Deletion, Chromosome Disorders, Genes, Immunoglobulin, Humans, Karyotyping, Lymphocytes immunology, Lymphoma, B-Cell blood, Lymphoma, B-Cell genetics, Lymphoma, B-Cell immunology, Male, Precursor Cell Lymphoblastic Leukemia-Lymphoma blood, Precursor Cell Lymphoblastic Leukemia-Lymphoma genetics, Precursor Cell Lymphoblastic Leukemia-Lymphoma immunology, Bone Marrow pathology, Burkitt Lymphoma pathology, Lymphocytes pathology, Lymphoma, B-Cell pathology, Precursor Cell Lymphoblastic Leukemia-Lymphoma pathology
- Abstract
A patient is described who presented with a chronic lymphocytic leukemia (CLL) and later developed a lymphoblastic lymphoma. The cells from the CLL were typical mature B lymphocytes as could be assessed by morphologic, cytochemical, and surface marker analyses. The cells from the lymphoblastic lymphoma were immature B cells that expressed CD10, CD20, and HLA-DR markers, but not surface Ig or cytoplasmic mu chains, and were negative for terminal deoxynucleotidyl transferase (TdT). The cells of two continuous cell lines, obtained from the bone marrow and the peripheral blood of the patient, had the same phenotype as the lymphoblastic lymphoma cells, did not contain the Epstein-Barr virus genome, and displayed malignant features in vitro, including the capacity to form colonies in agar. The two cell lines also shared identical chromosomal abnormalities, a finding which suggests that they derived from the same malignant cell already present in vivo. Such chromosomal abnormalities were not seen in the karyotype of the peripheral blood cells at the onset of the disease. Analysis of the Ig heavy chain genes using a DJ-specific probe showed the very same monoclonal rearrangement in the cells from the B-CLL, the lymphoblastic lymphoma and the two cell lines, thus demonstrating their common clonal origin. By contrast, a monoclonal rearrangement of the lambda chain gene locus was found in the B-CLL cells only, a finding consistent with their exclusive capacity to express surface IgM lambda. This patient represents a rare case in whom a chronic lymphoproliferative disorder with mature malignant cells transforms into a lymphoblastic lymphoma characterized by cells frozen at a very early maturational stage. The possible mechanisms leading to such transformation within the same cell clone are discussed.
- Published
- 1991
3. Immunoreactive lactoferrin in resting, activated, and neoplastic lymphocytes.
- Author
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Butler TW, Grossi CE, Canessa A, Pistoia V, and Barton JC
- Subjects
- B-Lymphocytes immunology, B-Lymphocytes metabolism, Cell Membrane metabolism, Cells, Cultured, Flow Cytometry, Fluorescent Antibody Technique, Humans, Lactoferrin immunology, Leukemia, Lymphocytic, Chronic, B-Cell metabolism, Lymphocyte Activation, Lymphocytes immunology, T-Lymphocytes immunology, T-Lymphocytes metabolism, Lactoferrin metabolism, Lactoglobulins metabolism, Leukemia, Lymphoid metabolism, Lymphocytes metabolism
- Abstract
Lactoferrin (Lf) in lymphocytes was assessed with immunofluorescence/flow cytometric technique. Surface Lf was detected primarily among B-cell-enriched preparations. Tonsillar B-cells of different densities expressed surface Lf similarly. Very small percentages of CALLA+ ALL, HCL, or EBV-transformed B-cells expressed surface Lf, whereas B-CLL lymphocytes had the highest percentages of surface Lf positivity. Few resting, cultured, or neoplastic T-lymphocytes expressed Lf. The pattern of immunofluorescence and analyses of surface and total cellular immunoreactive Lf indicated that Lf is associated primarily with the lymphocyte surface. The percentage and/or intensity of surface Lf-specific fluorescence were not significantly altered in B- or T-cells by incubation with physiologic concentrations of differric Lf, and the percentages of Lf-positive cells detected in respective subjects remained stable over time. Surface Lf positivity was unrelated to the expression of other surface antigens (except those marking B- or T-cell lineage) or cell cycle. Expression and/or binding of Lf in B-lymphocytes may become increased during certain stages of cell maturation.
- Published
- 1990
- Full Text
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4. Analysis of human peripheral blood lymphocytes isolated by counterflow centrifugation-elutriation.
- Author
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Franzi AT, D'Anna F, Cadoni A, Leprini A, Di Primio R, and Grossi CE
- Subjects
- B-Lymphocytes cytology, Centrifugation methods, Countercurrent Distribution, Humans, Killer Cells, Natural cytology, Monocytes cytology, T-Lymphocytes classification, Cell Separation methods, Lymphocytes cytology
- Abstract
Human peripheral blood mononuclear cells isolated by Ficoll-Hypaque density gradient centrifugation have been fractionated by counterflow centrifugal elutriation (CCE). Six CCE fractions were obtained and subsequently analyzed as for their content of monocytes, T cells, NK cells and B cells. The various cell types were identified through the expression of specific surface membrane determinants or by cytochemical staining for alpha-naphthyl acid esterase (ANAE). Monocytes were elutriated at the highest counterflow rates whereas the majority of B cells were collected at the lowest counterflow rates. T cells as well as NK cells were mostly concentrated in the intermediate fractions. No differences in the elutriation profile of T cells with the helper-inducer or with the cytotoxic-suppressor surface phenotype were observed. However, the percentages of T cells as determined by surface marker expression decreased with increasing counterflow rates, whereas the percentage of ANAE-positive T cells increased. Yet, T cells recovered at the high counterflow rates had ANAE-reactive organelles larger than those of T cells collected at low counterflow rates. These findings suggest that T cells at different maturational stages could be separated by CCE.
- Published
- 1983
- Full Text
- View/download PDF
5. Ultrastructure and cytochemistry of human peripheral blood lymphocytes. Similarities between the cells of the third population and TG lymphocytes.
- Author
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Ferrarini M, Cadoni A, Franzi AT, Ghigliotti C, Leprini A, Zicca A, and Grossi CE
- Subjects
- Histocytochemistry, Humans, Hydrolases, Lymphocytes classification, Monocytes immunology, Receptors, Antigen, B-Cell, Receptors, Fc, Immunoglobulin G, Lymphocytes ultrastructure, Receptors, Immunologic, T-Lymphocytes
- Abstract
The ultrastructural and cytochemical features of human peripheral blood TG cells (T cells with receptors IgG) and of the cells of the so-called third population (non-T, non-B cells with high avidity receptors for IgG) have been investigated and compared. Both TG and third-population cells (TPC) contained acid hydrolases with a paranuclear localization of alpha-naphthyl acid esterase, beta-glucuronidase or acid phosphatase. At the electron microscopy level, TG and TPC were indistinguishable and displayed rough cell surface, indented nuclei, abundant cytoplasm with predominance of the smooth over the rough membranes and peroxidase-negative granules. A large proportion of cells of the TPC could form rosettes with sheep erythrocytes after treatment with neuraminidase. The observed close similarities between TG and TPC may suggest that both cell types belong to a special subset of T cells. However, the alternative hypothesis that both TG and TPC are part of a subset unrelated to T cells, such as a new non-T, non-B cell population, or even of the monocytic-macrophage lineage, is also discussed.
- Published
- 1980
- Full Text
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6. Separation and characterization of cortical and medullary bursal lymphocytes.
- Author
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Grossi CE, Casali AM, Bartoli S, Governa M, and Manzoli FA
- Subjects
- Animals, Bursa of Fabricius cytology, Cell Membrane, Cell Separation, Chickens, DNA analysis, Fluorescent Antibody Technique, Immunoglobulins analysis, Photometry, Staining and Labeling, Thymidine metabolism, Tritium, Bursa of Fabricius immunology, Lymphocytes immunology
- Published
- 1974
- Full Text
- View/download PDF
7. Complement receptor distinguishes between two subsets of large granular lymphocytes with different natural killer activity and cytochemical and ultrastructural features.
- Author
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Nocera A, Montesoro E, Balbo P, Ferrarini M, Leprini A, Zicca A, and Grossi CE
- Subjects
- Antibodies, Monoclonal immunology, Histocytochemistry, Humans, Killer Cells, Natural ultrastructure, Lymphocytes ultrastructure, Killer Cells, Natural classification, Lymphocytes classification, Receptors, Complement immunology
- Abstract
Human peripheral blood large granular lymphocytes (LGL)--that is, cells with intracytoplasmic azurophilic (electron-dense) granules, with a positivity for the cytochemical localization of certain acid hydrolases, and with avid surface receptors for the Fc portion of IgG--have been purified on Percoll density gradients. Approximately 30% of these cells expressed receptors for the third complement component (C3R). They were separated into C3R-positive and C3R-negative cells. C3R+ cells had a significantly greater natural killer (NK) activity against K562 target cells than C3R+ cells. This difference was unrelated to the presence in the C3R+ cells of a contaminant cell type incapable of NK activity, since cytochemical and ultrastructural analysis revealed that C3R+ and C3R- fractions contained comparable LGL numbers. Agarose cytotoxicity assays at the single-cell level demonstrated that C3R+ LGL contained a large number of cells that bound to but did not lyse the target. The remaining fully cytotoxic C3R+ LGL had, however, the same killing and recycling properties as the cells from the C3R fraction. Electron microscopy and cytochemical studies showed that C3R+ cells had fewer electron-dense granules than C3R cells and stained more faintly for the localization of alpha-naphtyl acetate esterase. In contrast to C3R cells, C3R+ LGL displayed morphological features suggesting that an active process of granule formation was taking place. Taken together, the data indicate that C3R+ cells represent a discrete subset or a maturational stage of LGL.
- Published
- 1983
- Full Text
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8. Isolation and characterization of Leu 7+ germinal-center cells with the T helper-cell phenotype and granular lymphocyte morphology.
- Author
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Velardi A, Tilden AB, Millo R, and Grossi CE
- Subjects
- Adult, Antigens, Differentiation, T-Lymphocyte, Cell Separation, Cytotoxicity, Immunologic, Fluorescent Antibody Technique, Humans, Immunity, Cellular, Lymph Nodes immunology, Lymphocytes ultrastructure, Microscopy, Electron, Palatine Tonsil immunology, T-Lymphocytes, Helper-Inducer ultrastructure, Antigens, Surface analysis, Cytoplasmic Granules ultrastructure, Lymphocytes immunology, T-Lymphocytes, Helper-Inducer immunology
- Abstract
Leu 7+ cells in germinal centers of lymphoid tissues largely (greater than 90%) coexpress the T helper-cell marker, Leu 3. In this study we have isolated Leu 7+ (Leu 3+) cells from pharyngeal and palatine tonsils and we have analyzed their surface phenotype, morphologic and cytochemical characteristics, and functional properties. All of these features have been compared with those of T helper-cell populations with natural killer (NK)-like characteristics that we have previously described in peripheral blood. Leu 7+ (Leu 3+) cells from tonsil germinal centers display morphological and cytochemical features of granular lymphocytes and express the T3 marker in the absence of Leu 15. Following stimulation with phytohemagglutinin (PHA) or anti-T3, Leu 7+ (Leu 3+) cells express interleukin-2 (IL-2) receptors and proliferate to some extent in response to IL-2. The localization of Leu 7+ (Leu 3+) cells in B-dependent areas of lymphoid tissues suggests that they may play a regulatory role in B-cell proliferation and/or differentiation. Here we show that Leu 7+ (Leu 3+) cells do not produce B-cell growth factor (BCGF) and do not help pokeweed mitogen (PWM)-driven B-cell differentiation. Therefore, Leu 7+ (Leu 3+) germinal-center cells are distinct from "classic" T-helper cells of blood and lymphoid tissues.
- Published
- 1986
- Full Text
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9. [Immunohistochemical localization of immunglobulins from the lymphocyte membrane of chickens].
- Author
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Governa M, Grossi CE, and Manzoli FA
- Subjects
- Animals, Antibody Formation, Bursa of Fabricius immunology, Cell Membrane immunology, Chickens, Fluorescent Antibody Technique, Immunoglobulins isolation & purification, Lymphocytes immunology
- Published
- 1974
10. Rheumatoid arthritis associated with expanded populations of granular lymphocytes.
- Author
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Barton JC, Prasthofer EF, Egan ML, Heck LW Jr, Koopman WJ, and Grossi CE
- Subjects
- Aged, Antibody-Dependent Cell Cytotoxicity, Arthritis, Rheumatoid immunology, Blood Cell Count, Bone Marrow pathology, Cyclophosphamide therapeutic use, Female, Humans, Killer Cells, Natural physiology, Lymphocytes classification, Lymphocytosis drug therapy, Male, Methotrexate therapeutic use, Middle Aged, Neutropenia immunology, Phenotype, Prednisone therapeutic use, Rheumatoid Factor analysis, Arthritis, Rheumatoid blood, Lymphocytes pathology, Lymphocytosis immunology
- Abstract
Two patients with classic rheumatoid arthritis developed severe neutropenia and increased numbers of large granular lymphocytes in the blood and bone marrow. These lymphocytes exhibited homogeneous surface membrane immunophenotypes of Leu5+, Leu11-, Leu4+, Leu3-, Leu2-, Leu7+ and Leu5+, Leu11+, Leu4+, Leu3-, Leu2+, Leu7-, respectively. In both patients, neutropenia was initially corrected with corticosteroid therapy; long-term improvement followed low-dose oral cyclophosphamide and methotrexate therapies. In these 2 patients and 12 previous patients with rheumatoid arthritis associated with expanded populations of immunophenotypically homogeneous large granular lymphocytes, neutropenia occurred in all 14, thrombocytopenia in 6, anemia in 7, and mild or moderate splenomegaly in 12. In contrast to Felty's syndrome, granular lymphocyte expansions in rheumatoid arthritis usually occur in older patients, may appear simultaneously with arthritis, and are usually associated with normal or elevated blood leukocyte counts. Mild hemocytopenias in these patients can often be managed with observation. Therapy with corticosteroids or immunosuppressive-cytotoxic drugs may be beneficial in more severe cases, but splenectomy is not recommended.
- Published
- 1986
- Full Text
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11. Cytophotometric characterization of cortical and medullary lymphocytes in the chicken bursa and thymus.
- Author
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Casali AM, Grossi CE, Manzoli Guidotti L, and Manzoli FA
- Subjects
- Animals, B-Lymphocytes analysis, Cell Nucleus analysis, DNA analysis, Histones analysis, Nucleic Acids analysis, Photometry, T-Lymphocytes analysis, Bursa of Fabricius cytology, Chickens anatomy & histology, Lymphocytes analysis, Thymus Gland cytology
- Abstract
We have evaluated by means of cytophotometric techniques the nuclear content in DNA, in total nucleic acids and in histone proteins and the nuclear volume of the thymic and bursal lymphocytes in adult chickens. We have also made the same determinations in the developing bursal follicles before and after hatching. The results indicate that cortical differ from medullary lymphocytes for all the parameters considered both in thymic lobules and bursal follicles. Furthermore these differences appear analogous in both the organs, independently from the fact that they produce precursors of T and B lymphocytes respectively. As concerns the developing bursal follicle, the lymphocytes show the characteristics of the adult medullary lymphocytes. At the hatching changes occur in the nuclear content of total nucleic acids and histones. This is probably related to the exposure to antigenic stimulation through the cloaca.
- Published
- 1977
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12. Large granular lymphocytes from patients with expanded LGL populations acquire cytotoxic functions and release lymphokines upon in vitro activation.
- Author
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Pistoia V, Prasthofer EF, Tilden AB, Barton JC, Ferrarini M, Grossi CE, and Zuckerman K
- Subjects
- Antibodies, Monoclonal, Antigens, Surface analysis, Cell Differentiation, Humans, Immunity, Cellular, Immunity, Innate, Interleukin-2 immunology, Lymphocyte Activation, Lymphocytes classification, Rosette Formation, Cytotoxicity, Immunologic, Killer Cells, Natural immunology, Lymphocytes immunology, Lymphokines metabolism
- Abstract
The phenotypic and functional features of purified large granular lymphocytes (LGL) from ten patients with LGL population expansions and cytopenias are described. The predominant LGL phenotypes were T3+, T8+, Leu-11+/-; however, in two patients, LGL expressed a T3-, Leu-11+ phenotype. Variable combinations of other LGL markers (OKM1, Leu-7), and HLA-DR were detected in individual cases. In nine of ten cases, freshly isolated LGL did not exert cytolytic activity for K562 target cells, but purified LGL cultured in the presence of recombinant interleukin 2 (rIL2) acquired potent cytotoxic activity in all cases tested. LGL did not proliferate in response to phytohemagglutinin (PHA). However, LGL released variable amounts of IL2 and gamma-interferon (gamma-IFN) after PHA stimulation. In some cases, stimulation of fresh LGL with recombinant IL2 induced production of gamma-IFN. No correlation was found between the functional capabilities and the original phenotype of the expanded LGL populations.
- Published
- 1986
13. Granule formation in large granular lymphocytes (LGL). Ultrastructural and cytochemical studies on cells from patients with abnormally expanded lgl populations.
- Author
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Grossi CE, Zicca A, Leprini A, Cadoni A, Pandolfi F, Burgio VL, Romagnani S, Guglielmi C, and Ferrarini M
- Subjects
- Acid Phosphatase analysis, Adult, Aged, Female, Humans, Leukemia, Lymphoid enzymology, Leukocyte Count, Lymphocytes cytology, Lymphocytes enzymology, Male, Microscopy, Electron, Middle Aged, Thiamine Pyrophosphatase analysis, Cytoplasmic Granules ultrastructure, Leukemia, Lymphoid pathology, Lymphocytes ultrastructure
- Abstract
The peripheral blood mononuclear cells from five patients with abnormally expanded populations of large granular lymphocytes (LGL) of possible malignant origin have been studied by electron microscopy. These cells had fewer granules than normal circulating LGL but displayed a variety of organelles possibly involved in the process of granule formation. This process apparently originated from the fusion of vesicles that had a characteristic cup-shape (and appeared to be derived from the Golgi apparatus) with smaller vesicles, mostly of the coated type. This fusion resulted in the formation of multivesicular bodies (MVB) whose limiting membrane was constituted by that of the cup-shaped vesicle. MVB gradually matured into electron-dense granules. A number of cup-shaped vesicles were found positive for the cytochemical localization of thiamine pyrophosphatase (TPP) activity that is a specific marker of the trans aspect of the Golgi apparatus, a finding which confirmed that they were Golgi-derived. The smaller, mostly coated, vesicles were positive for acid phosphatase (AP) and evidence was obtained indicating that they subserved the function of transporting acid hydrolases from the rough endoplasmic reticulum to the cup-shaped vesicles or to the MVB. The plasma membrane did not appear to contribute to MVB or granule formation since endocytic vacuoles induced by reacting the cell surface with the appropriate monoclonal antibodies (followed by peroxidase labelled anti-immunoglobulin) were never seen while fusing with granules or MVB. Rather, these vesicles formed secondary lysosomes after having encountered smooth or, more rarely, coated vesicles. Granulogenesis in LGL, therefore, resembles the process of granule formation observed in secretory cells. The active process of granulogenesis detected in the patient cells and a number of other findings indicate that they were LGL at early stages of maturation. The surface phenotype consistently found in these cells (OKT3+, Leu 1+, OKT8+, Leu 2a+, OKT11+, Leu 5+ and OKM1) is different from that of the majority of the normal LGL and may correspond to that of immature LGL.
- Published
- 1984
14. Ultrastructural morphology of granular lymphocytes (GL) from patients with immunophenotypically homogeneous expansions of GL populations (GLE).
- Author
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Prasthofer EF, Barton JC, Zarcone D, and Grossi CE
- Subjects
- Antibodies, Monoclonal, Antigens, Surface analysis, Cytoplasmic Granules ultrastructure, Humans, Lymphocytes classification, Lymphocytes immunology, Microscopy, Electron, T-Lymphocytes classification, T-Lymphocytes immunology, Lymphocytes ultrastructure, Lymphoproliferative Disorders pathology, T-Lymphocytes ultrastructure
- Abstract
We studied the morphology of large granular lymphocytes (GL) from 19 patients with expanded populations of GL (GLE) of homogeneous surface immunophenotype. By definition, all patients had increased numbers of medium-sized lymphocytes with azurophilic granules, and neutropenia, thrombocytopenia, and/or anemia. In all cases, the GL had an extended Golgi apparatus and prominent centrioles. Among cells from individual patients, the GL were homogeneous with respect to their granule morphology. GL containing parallel tubular arrays (PTA), commonly associated with GLE, were observed in 9 of 19 cases; granules containing scarce matrix with or without associated vesicles (SMG) were present in the remaining 10 cases. In all cases in which the cells had PTA, there was co-expression of CD3 and CD8, the T suppressor cell-associated phenotype, whereas only 4 of 10 cases with SMG expressed CD3 and CD8 (p less than 0.01, chi-square test). We conclude that the morphologic homogeneity of GL in individual patients with GLE differs significantly from the heteromorphism usually seen in reactive GL, and is consistent with, but not diagnostic of, a clonal proliferation of GL in persistent GLE.
- Published
- 1987
15. Establishment of Tac-negative, interleukin-2-dependent cytotoxic cell lines from large granular lymphocytes (LGL) of patients with expanded LGL populations.
- Author
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Pistoia V, Carroll AJ, Prasthofer EF, Tilden AB, Zuckerman KS, Ferrarini M, and Grossi CE
- Subjects
- Antigens, Surface isolation & purification, Cell Line, Chromosome Aberrations, Cytotoxicity, Immunologic, Humans, Interleukin-2 immunology, Lymphocytes immunology, Tumor Necrosis Factor Receptor Superfamily, Member 7, Lymphocytes cytology
- Abstract
Cell lines were established from purified large granular lymphocytes (LGL) isolated from the peripheral blood of seven patients with phenotypically homogeneous LGL expansions. LGL were stimulated with phytohemagglutinin (PHA) or recombinant interleukin-2 (rIL-2) and further expanded in vitro in IL-2-containing media. The surface phenotype of LGL, as assessed by monoclonal antibody staining, was T3+ T8+ in five patients, T3- T8- in one, and T3+ T8- in another patient. The cells also expressed Leu 7, Leu 11, and/or OKM 1 markers in various proportions and were identifiable as LGL by their morphological and cytochemical features. The original surface phenotype of the unstimulated LGL was retained in the IL-2-dependent cell lines from each individual patient, i.e., T3+ T8+ cells originated T3+ T8+ cell lines and T3- T8- cells originated T3- T8- cell lines. Other markers, such as Leu 11 and OKM 1, were generally lost in culture. LGL proliferated in response to rIL-2 but did not express detectable IL-2 receptors, even after prolonged periods of culture. All cell lines from each individual patient had the same surface phenotype, and within the single lines, all of the cells expressed the same markers. Cell lines from two patients consistently displayed chromosomal abnormalities. Although different in the two patients, the abnormalities were identical in all of the lines from the same patient and detectable in most of the cells examined, suggesting a clonal origin for the abnormally expanded LGL populations. Freshly isolated LGL did not exert NK activity. However, the IL-2-dependent LGL lines acquired the ability to kill K562 target cells and to produce gamma interferon (gamma-IFN). No direct correlation was observed between these two properties.
- Published
- 1986
- Full Text
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16. Medullary localization of extracellular immunoglobulin and aminopeptidase in lymphoepithelial follicles of the chicken bursa of fabricius and rabbit appendix.
- Author
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Grossi CE, Lydyard PM, and Cooper MD
- Subjects
- Animals, Appendix enzymology, Bursa of Fabricius analysis, Bursa of Fabricius enzymology, Chickens, Fluorescent Antibody Technique, Rabbits, Aminopeptidases isolation & purification, Appendix immunology, Bursa of Fabricius immunology, Immunoglobulin M, Lymphocytes immunology
- Published
- 1977
- Full Text
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17. A lymphoproliferative disorder of the large granular lymphocytes with natural killer activity.
- Author
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Ferrarini M, Romagnani S, Montesoro E, Zicca A, Del Prete GF, Nocera A, Maggi E, Leprini A, and Grossi CE
- Subjects
- Antibody-Dependent Cell Cytotoxicity, Cell Differentiation, Cytoplasmic Granules ultrastructure, Humans, In Vitro Techniques, Killer Cells, Natural ultrastructure, Lymphocytes ultrastructure, Lymphoproliferative Disorders pathology, Male, Middle Aged, Killer Cells, Natural immunology, Lymphocytes immunology, Lymphoproliferative Disorders immunology
- Published
- 1983
- Full Text
- View/download PDF
18. Human lymphocyte subpopulations.
- Author
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Vogler LB, Grossi CE, and Cooper MD
- Subjects
- Animals, B-Lymphocytes immunology, Cell Membrane enzymology, Cytoplasm enzymology, Cytoplasm immunology, Genes, MHC Class II, HLA Antigens immunology, Herpesvirus 4, Human immunology, Humans, Lymphocytes drug effects, Lymphocytes enzymology, Mice, Nucleotidyltransferases, Receptors, Antigen, B-Cell, Receptors, Complement immunology, Receptors, Fc immunology, Receptors, Virus immunology, Rosette Formation, Sheep, T-Lymphocytes immunology, Lymphocytes classification
- Published
- 1979
19. Large granular lymphocytes in human peripheral blood: ultrastructural and cytochemical characterization of the granules.
- Author
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Grossi CE, Cadoni A, Zicca A, Leprini A, and Ferrarini M
- Subjects
- Acid Phosphatase blood, Adult, Cytoplasmic Granules enzymology, Histocytochemistry, Humans, Lymphocytes enzymology, Lymphocytes physiology, Naphthol AS D Esterase blood, Phagocytosis, Cytoplasmic Granules ultrastructure, Lymphocytes ultrastructure
- Abstract
Large granular lymphocytes (LGL) are defined as nonadherent mononuclear cells with cytoplasmic azurophilic granules, avid receptors for the Fc portion of IgG, and cytotoxic functions (NK or ADCC activities). In the present study, the granules of LGL isolated from human peripheral blood have been analyzed by enzyme cytochemistry and electron microscopy. It had been found that: (1) in the single cells, granules at different stages of maturation could be detected: in addition, packaging of the granules took place in the proximity of the Golgi apparatus, which is similar to that seen in secretory cell types. (2) Acid phosphatase (AP) was observed within the granules and the vesicles located in the Golgi area: the Golgi apparatus identified through its thiamine pyrophosphatase-positivity was consistently negative for AP. (3) Alpha naphthyl-acetate esterase (ANAE) activity was localized in the granules as well as on the membrane of LGL and monocytes. (4) The ANAE activity of LGL was of the monocytic and not of the lymphocytic type, as shown by NaF inhibition. (5) The LGL granules, although identifiable as primary lysosomes, were not involved in the process of phagocytosis, since LGL failed consistently to ingest latex particles or opsonized red cells.
- Published
- 1982
20. Expression of the Chediak-Higashi lysosomal abnormality in human peripheral blood lymphocyte subpopulations.
- Author
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Grossi CE, Crist WM, Abo T, Velardi A, and Cooper MD
- Subjects
- Adult, Antibodies, Monoclonal immunology, B-Lymphocytes pathology, Cell Differentiation, Chediak-Higashi Syndrome immunology, Child, Cytotoxicity, Immunologic, Humans, Hydrolases metabolism, Killer Cells, Natural pathology, Lymphocyte Activation, Lymphocytes enzymology, Male, T-Lymphocytes, Helper-Inducer pathology, Chediak-Higashi Syndrome blood, Lymphocytes classification, Lysosomes
- Abstract
Fusion of lysosomes to form a giant cytoplasmic inclusion is a major abnormality expressed by multiple hematopoietic and non-hematopoietic cell types in Chediak-Higashi (C-H) patients. In this study, the extent of involvement of lymphoid cell subpopulations was defined. Purified populations of B cells, natural killer (NK) cells, and helper T cells were obtained from two C-H patients and normal controls by immunofluorescence staining of their blood mononuclear cells with the monoclonal antibodies HB-2, Leu-7, or Leu-3 followed by fluorescence-activated cell sorting. Cytochemical and ultrastructural analyses as well as functional assays were performed to determine whether or not the C-H lysosomal abnormality was expressed in the different lymphocyte subpopulations. B cells expressed the C-H defect following activation and differentiation. All of the Leu-7+ cells and a significant proportion of the Leu-3+ cells displayed the C-H abnormality. These Leu-3+ cells share the NK lineage characteristics of granular lymphocyte morphology and the capacity to bind to NK cell targets. In contrast, the C-H abnormality was not observed in non-NK target-binding cells with T helper phenotype, in which clusters of lysosomes formed a normal Gall body. Moreover, T cell functions were unimpaired in C-H patients. These observations raise the issue of the lineal relationship between granular and nongranular lymphocytes typed as T cells on the basis of cell surface antigen markers.
- Published
- 1985
21. Subpopulation analysis of human granular lymphocytes: associations with age, gender and cytotoxic activity.
- Author
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Tilden AB, Grossi CE, Itoh K, Cloud GA, Dougherty PA, and Balch CM
- Subjects
- Adolescent, Adult, Age Factors, Aged, Antibodies, Monoclonal, Antigens, Surface analysis, Cell Line, Child, Child, Preschool, Cytotoxicity, Immunologic, Female, Humans, Immunity, Cellular, Immunity, Innate, Killer Cells, Natural classification, Killer Cells, Natural cytology, Lymphocytes immunology, Lymphocytes ultrastructure, Lymphokines immunology, Male, Melanoma immunology, Middle Aged, Sex Factors, Killer Cells, Natural immunology, Lymphocytes classification
- Abstract
Levels of human blood granular lymphocyte subpopulations were enumerated in 105 healthy donors ranging in age from 0 to 79 years. Using double-label immunofluorescence, subpopulations of granular lymphocytes were enumerated as follows: Leu7+Leu2+, Leu7+Leu3+, Leu7+11-, Leu7+77+ and Leu7-Leu11+. The proportion of cells with granular lymphocyte morphology was determined by Giemsa staining. Natural killer (NK) cell activity against K-562 cells and lymphokine-activated killer activity against non-cultured melanoma cells were examined in parallel. Levels of total Leu7+ and Leu11+ cells increased with age (p = 0.0001) and were higher in males than females (p = 0.001). The total number of cells with granular lymphocyte morphology had an age-related increase (p = 0.001), but were not significantly higher in males than in females (p = 0.07). There was no selective increase in one granular lymphocyte subpopulation versus another since the Leu7+Leu11- (p = 0.0001), the Leu7+Leu11+ (p = 0.0001), the Leu7+Leu2+ (p = 0.0001) and the Leu7+Leu3+ (p = 0.0004) all had similar age-related increases. The one exception was the Leu7-Leu11+ (p = 0.1) granular lymphocyte subset which was low in the first decade of life but had reached maximum levels in the second decade. NK cell activity against K-562 cells was moderately increased with age (p = 0.06) with males and females exhibiting comparable activity. In contrast. lymphokine-activated killer cytotoxicity of non-cultured melanoma cells was similar in all age groups. NK cell activity was highly correlated with levels of morphologically defined granular lymphocytes (p = 0.005) and moderately with total Leu11+ cells (p = 0.06) but not with other subpopulations of granular lymphocytes.
- Published
- 1986
22. A lymphoproliferative disorder of granular lymphocytes with a novel phenotype and suppressor function.
- Author
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Landay A, Poon MC, Clement LT, and Grossi CE
- Subjects
- Adult, Cytoplasmic Granules, Cytotoxicity, Immunologic, Histocytochemistry, Humans, Killer Cells, Natural immunology, Lymphocytes immunology, Lymphocytes pathology, Lymphoproliferative Disorders immunology, Lymphoproliferative Disorders metabolism, Male, Phenotype, T-Lymphocytes, Regulatory immunology, Lymphocyte Activation, Lymphocytes classification
- Abstract
In this study we have identified and characterized an expanded granular lymphocyte population in a patient with anemia and granulocytopenia. Granular lymphocytes were identified through the presence of cytoplasmic azurophilic granules, the dispersed granular pattern of cytochemical staining for acid hydrolases, and the ultrastructural localization of acid phosphatase within the granules. The surface phenotype of the granular lymphocytes was E+, FcR-, Leu 4+, Leu 2+, D 12+ (Leu 15+), OKM1+, and Leu 7+. This phenotype has not been reported previously in patients with similar features. Functional studies on FACS-purified populations showed that the patient's granular lymphocytes responded poorly to T-cell mitogens and were inefficient in NK and ADCC assays but exerted a potent suppressor effect on both T-cell proliferation and B-cell differentiation. The phenotype and functions of the expanded granular lymphocyte population correspond to those of a subset of normal E rosette-forming granular lymphocytes.
- Published
- 1984
- Full Text
- View/download PDF
23. A large subpopulation of lymphocytes with T helper phenotype (Leu-3/T4+) exhibits the property of binding to NK cell targets and granular lymphocyte morphology.
- Author
-
Velardi A, Grossi CE, and Cooper MD
- Subjects
- Acid Phosphatase analysis, Adolescent, Adult, Aged, Antibodies, Monoclonal, Cell Line, Cytotoxicity, Immunologic, Fluorescein-5-isothiocyanate, Fluoresceins, Fluorescent Antibody Technique, Fluorescent Dyes, Genetic Markers, Humans, Immunoenzyme Techniques, Leukemia, Myeloid, Acute, Lymphocytes ultrastructure, Microscopy, Electron, Middle Aged, Phenotype, Thiocyanates, Antigens, Surface analysis, Killer Cells, Natural immunology, Lymphocytes immunology, T-Lymphocytes, Helper-Inducer immunology
- Abstract
A discrete subpopulation of lymphocytes sharing several phenotypic characteristics with natural killer (NK) cells was identified within the circulating pool of human lymphocytes that bear the T helper marker Leu-3. This Leu-3+ subpopulation of cells formed cell conjugates with the NK target cell lines K562 and MOLT-4, but did not bind to mouse myeloma and hybridoma cell lines that are insensitive to NK cells. The Leu-3+ lymphocytes binding to NK cell targets contained cytoplasmic granules similar in ultrastructure and cytochemistry to those previously defined in granular lymphocytes with NK function, except that the granules in Leu-3+ cells were smaller and fewer in number. Unlike classical NK cells, however, the granular Leu-3+ cells did not kill the target cells to which they bound, even after treatment with interferon. The proportion of granular Leu-3+ cells with the capacity to bind to NK cell targets was approximately 7% at birth and increased to approximately 21% of the Leu-3+ cells in adults. These observations suggest the possibility of a lineal relationship between the granular Leu-3+ cells and granular Leu-3- cells with NK capability.
- Published
- 1985
24. [COMPARATIVE STUDY OF INFECTIOUS MONONUCLEOSIS IN RELATION TO THE AGE OF THE SUBJECT].
- Author
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ROBERT L and GROSSI CE
- Subjects
- Adolescent, Child, Humans, Infant, Aging, Antibodies, Bone Marrow Examination, Diagnosis, Differential, Infectious Mononucleosis, Leukocyte Count, Lymphocytes, Pathology, Radiography, Thoracic, Serologic Tests, Tuberculin Test
- Published
- 1964
25. Cytophotometric characterization of nuclei in chicken bursal lymphocytes.
- Author
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Grossi CE, Casali AM, Manzoli FA, and Manzoli-Guidotti L
- Subjects
- Aging, Animals, Bursa of Fabricius cytology, Chickens, Coloring Agents analysis, Lymphocytes cytology, Male, Nucleic Acids analysis, Staining and Labeling, Bursa of Fabricius growth & development, Cell Nucleus analysis, Lymphocytes analysis
- Published
- 1972
- Full Text
- View/download PDF
26. Tγδ-Cell Subsets in Cord and Adult Blood.
- Author
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Smith, M. D., Worman, C., Yüksel, F., Yüksel, B., Moretta, L., Ciccone, E., Grossi, C. E., Mackenzie, L., and Lydyard, P. M.
- Subjects
CELLS ,IMMUNOGLOBULINS ,CORD blood ,LYMPHOCYTES ,MOLECULAR cloning ,MONOCLONAL antibodies ,ENZYMES - Abstract
A minor population of T cells expresses a heterodimeric antigen receptor composed of γ and &delta chain (TcR-1). In blood from adults, two subsets pf Tγ&delta cells can be identified by the monoclonal antibodies (MoAb) BB3 and A13. Little is known about the distribution and markers of these subsets early in life. We have therefore examined both the frequencies of these cells in cord blood and their expression of the cytotoxicity-associated marker serine esterase (SE), using immunocytochemical techniques. Our data show lower percentages of TcR-1
+ cells in the blood of newborns compared with that in adults. However, the ratio of the A13+ /BB3+ cells was significantly higher in cord than in adult blood. Whereas virtually all the adult TcR-1+ cells in blood were SE-positive, only a small proportion of the cord blood cells carried this enzyme. This was restricted to the BB3+ Tγδ-cells subset in the cord. Our data suggest different characteristic of the TcR-1+ cells in blood from newborns compared with adult blood, and study of the functions of the different subsets, e.g. cytotoxicity, will be important in understanding their particular role in immunity. [ABSTRACT FROM AUTHOR]- Published
- 1990
- Full Text
- View/download PDF
27. Immune parameters identify Italian centenarians with a longer five-year survival independent of their health and functional status.
- Author
-
Bucci, L., Ostan, R., Giampieri, E., Cevenini, E., Pini, E., Scurti, M., Vescovini, R., Sansoni, P., Caruso, C., Mari, D., Ronchetti, F., Borghi, M.O., Ogliari, G., Grossi, C., Capri, M., Salvioli, S., Castellani, G., Franceschi, C., and Monti, D.
- Subjects
- *
IMMUNOLOGY , *PHENOTYPES , *AGING , *TYPE 2 diabetes , *T cells , *LYMPHOCYTES , *CD19 antigen , *PHYSIOLOGY - Abstract
Abstract: Centenarians are rare and exceptional individuals characterized by a peculiar phenotype. They are the best example of healthy aging in humans as most of them have escaped or substantially delayed the onset of major age-related diseases. Within this scenario, the purpose of the present work was to understand if immune status is associated with survival and health status in centenarians. To this aim, 116 centenarians were concomitantly characterized for their immunological, health and functional status, and followed-up for five-year survival. On the basis of previous knowledge we focused on a core of fundamental and basic immune parameters (number of leukocytes, monocytes, total lymphocytes, CD3+ T lymphocytes, CD4+ helper T lymphocytes, CD8+ cytotoxic T lymphocytes, CD19+ B lymphocytes and plasma levels of IgM), and the most important findings can be summarized as follows: i. a hierarchical cluster analysis was able to define Cluster1 (88 centenarians) and Cluster2 (28 centenarians) characterized by low and high values of all these immune parameters, respectively; ii. centenarians of Cluster2 showed a statistically longer five-year survival and more favorable values of other important immune (naïve, activated/memory and effector/memory T cells) and metabolic (glycemia, insulin and HOMA-IR) parameters, in accord with previous observations that centenarians have a peculiar immune profile, a preserved insulin pathway and a lower incidence of type 2 diabetes; and iii. unexpectedly, parameters related to frailty, as well as functional and cognitive status, did not show any significant correlation with the immune clustering, despite being capable per se of predicting survival. In conclusion, high values of basic immunological parameters and important T cell subsets correlate with five-year survival in centenarians, independent of other phenotypic characteristics. This unexpected biological scenario is compatible with the general hypothesis that in centenarians a progressive disconnection and loss of biological coherence among the different functions of the body occur, where survival/mortality result from the failure of any of these domains which apparently follow an independent age-related trajectory. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
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