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Start Over Author "Weidauer E" Topic lung neoplasms
2 results on '"Weidauer E"'

1. Response of rat alveolar type II cells and human lung tumor cells towards oxidative stress induced by hydrogen peroxide and paraquat.

Author

Weidauer E, Lehmann T, Rämisch A, Röhrdanz E, and Foth H

Subjects
ATP Binding Cassette Transporter, Subfamily B genetics, Animals, Catalase metabolism, Cell Line, Tumor, Humans, Hydrogen Peroxide toxicity, Lung Neoplasms pathology, Male, Malondialdehyde metabolism, Microscopy, Electron, Oxidative Stress physiology, Paraquat toxicity, Pulmonary Alveoli pathology, RNA genetics, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Superoxide Dismutase metabolism, ATP-Binding Cassette Sub-Family B Member 4, ATP Binding Cassette Transporter, Subfamily B metabolism, Hydrogen Peroxide pharmacology, Lung Neoplasms drug therapy, Lung Neoplasms metabolism, Paraquat pharmacology, Pulmonary Alveoli drug effects, Pulmonary Alveoli metabolism
Abstract

The expression of MDR1b coding mRNA is increased in alveolar type II cells from juvenile rat lung in culture. Hydrogen peroxide and paraquat-induced further upregulation supporting that oxidative stress mediated mechanisms are involved in the regulation of MDR1b in rat lung. The expression rates of mRNA for catalase, Cu/Zn-superoxide dismutase (Cu/Zn-SOD) and Mn-superoxide dismutase (Mn-SOD) remains constant during culture and were not modulated by hydrogen peroxide or paraquat. Thus, antioxidative enzymes in primary A II cells from rat lung are not regulated by reactive oxygen species dependent mechanisms. Primary A II cells were substantially more sensitive towards paraquat-induced cytotoxicity and lipid peroxidation than the permanent human lung tumor cell lines H322 and H358. A 100 microM hydrogen peroxide for 2h induces substantial DNA damage which is not paralleled by an increased rate of lipid peroxidation. The expression rate of mRNA coding for catalase and Mn-SOD was not changed and almost the same is true for the activity of catalase and Cu/Zn-SOD. Only 50 microM paraquat induced a significant decrease in catalase activity and an increase in Cu/Zn-SOD activity.

Published
2004
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2. Expression of MRP1 and related transporters in human lung cells in culture.

Author

Lehmann T, Köhler C, Weidauer E, Taege C, and Foth H

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, ATP-Binding Cassette Transporters genetics, ATP-Binding Cassette Transporters metabolism, Adult, Aged, Animals, Blotting, Western, Calcium Channel Blockers pharmacology, Epithelial Cells metabolism, Fluorescent Dyes metabolism, Humans, Immunohistochemistry, Individuality, Lung Neoplasms enzymology, Male, Middle Aged, Multidrug Resistance-Associated Proteins, Pulmonary Alveoli metabolism, RNA, Messenger biosynthesis, RNA, Messenger genetics, Rats, Reverse Transcriptase Polymerase Chain Reaction, Rhodamine 123 metabolism, Tumor Cells, Cultured, Verapamil pharmacology, ATP Binding Cassette Transporter, Subfamily B, Member 1 biosynthesis, ATP-Binding Cassette Transporters biosynthesis, Gene Expression Regulation, Neoplastic, Lung metabolism, Lung Neoplasms metabolism
Abstract

Multidrug resistance type 1 P-glycoproteins (P-gp) and multidrug resistance associated proteins (MRP) were studied in differentiated primary human lung cells in culture, in comparison with permanent human lung cell lines and primary alveolar type II cells from rat lung. AII cells exhibited low basal levels of mdr1b mRNA, that increased over time and after oxygen radical production induced by paraquat. mRNAs coding for antioxidative enzymes catalase (CAT), maganese superoxide dismutase (Mn-SOD) and copper/zinc superoxide dismutase (Cu/Zn-SOD) were not changed. H358, A549, H322 cells expressed low levels of MDR1 mRNA, but the mdr1 substrate rhodamine 123 (Rh 123) was transported out of H358 and H322 cells in a non-invasive, single cell fluorescence assay. The dye efflux could be inhibited by the chemosensitizer, verapamil. Normal human bronchial epithelial cells (NHBEC) expressed immuno-reactive MDR1 P-gp and the MPR protein that was active in the fluorescence assay using the MRP substrate carboxy-dichlorofluorescein (CDF) and MK-571 as an inhibitor. We did observe inter-individual variation of MRP in both the mRNA and the immunoreactive protein in NHBEC culture. Over time (12 weeks) the protein was relatively stable in NHBEC and epithelial cells from peripheral lung (PLC), but the mRNA level was drastically increased when explant cultures were continued (18 weeks).

Published
2001
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