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Objective To investigate the molecular testing of liquid-based cyiolof:,'Y ( LBC) specimens from advanced non-small ceil hmg cancer ( NSCLC) patients and the reliability of guiding targeted therapy. Methods The LBC spcx:imens ) had KnAS gene mutations, and 3 ( 0. 7%•) had BRAF gene mutations. EGFn mutation was associated with gender, pathologytype, and specimen source, with a higher EGFR mutation rate in fommac patients ( 63. 0%·) tlum in female patients ( 40.SCk, P<0.001) and a higher EGFR mutation rate in adenocarcinoma ( 54.3%·) thmi in non· adenocarcinoma ( 0.0%·, P

Ｏｂｊｅｃｔｉｖｅ 　Ｔｏ ｅｘｐｌｏｒｅ ｔｈｅ ｆｅａｓｉｂｉｌｉｔｙ ｏｆ ｂｒｏｎｃｈｏｓｃｏｐｉｃ ｂｒｕｓｈｉｎｇ ｌｉｑｕｉｄ⁃ｂａｓｅｄ ｓｌｉｄｅ ｃｙｔｏｌｏｇｙ ｃｏｍｂｉｎｅｄ ｗｉｔｈ ａｕｔｏｍａｔｉｃ ｉｍｍｕｎｏｃｙｔｏｃｈｅｍｉｓｔｒｙ （ ＩＣＣ） ｆｏｒ ｐａｔｈｏｌｏｇｉｃａｌ ｔｙｐｉｎｇ ｏｆ ｌｕｎｇ ｃａｎｃｅｒ． Ｍｅｔｈｏｄｓ　Ａ ｌｉｑｕｉｄ⁃ｂａｓｅｄ ｔｈｉｎ⁃ｐｒｅｐ ｗａｓ ｐｒｅｐａｒｅｄ ｆｒｏｍ １７１ ｂｒｏｎｃｈｏｓｃｏｐｉｃ ｂｒｕｓｈｉｎｇ ｓｐｅｃｉｍｅｎｓ ｏｆ ｐａｔｉｅｎｔｓ ｗｉｔｈ ｐｕｌｍｏｎａｒｙ ｌｅｓｉｏｎｓ． ＩＣＣ ｗａｓ ｄｅｔｅｃｔｅｄ ｂｙ ａｕｔｏｍａｔｉｃ ｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｓｔｒｙ ｉｎｓｔｒｕｍｅｎｔ ｗｈｉｌｅｃｙｔｏｍｏｒｐｈｏｌｏｇｉｃａｌ ｄｉａｇｎｏｓｉｓ ｗａｓ ｍａｄｅ． Ｔｈｅ ｒｅｓｕｌｔｓ ｗｅｒｅ ｃｏｍｐａｒｅｄ ｗｉｔｈ ｔｈｏｓｅ ｏｆ ｈｉｓｔｏｐａｔｈｏｌｏｇｉｃａｌ ｄｉａｇｎｏｓｉｓ． Ｒｅｓｕｌｔｓ　Ａｍｏｎｇ １７１ ｐａｔｉｅｎｔｓ， １３０ （７６．０％） ｃｏｕｌｄ ｂｅ ｃｌａｓｓｉｆｉｅｄ ｂｙ ｃｅｌｌ ｍｏｒｐｈｏｌｏｇｙ ａｌｏｎｅ， ｉｎｃｌｕｄｉｎｇ ３１ ｓｑｕａｍｏｕｓ ｃｅｌｌ ｃａｒｃｉｎｏｍａｓ， ４４ ａｄｅｎｏｃａｒｃｉｎｏｍａｓ ａｎｄ ５５ ｓｍａｌｌ ｃｅｌｌ ｃａｒｃｉｎｏｍａｓ； １６２ （９４． ７％） ｃｏｕｌｄ ｂｅ ｃｌａｓｓｉｆｉｅｄ ｂｙ ｃｅｌｌ ｍｏｒｐｈｏｌｏｇｙ ｃｏｍｂｉｎｅｄ ｗｉｔｈ ＩＣＣ， ｉｎｃｌｕｄｉｎｇ ３８ ｓｑｕａｍｏｕｓ ｃｅｌｌ ｃａｒｃｉｎｏｍａｓ， ６１ ａｄｅｎｏｃａｒｃｉｎｏｍａｓ ａｎｄ ６３ ｓｍａｌｌ ｃｅｌｌ ｃａｒｃｉｎｏｍａｓ （ Ｐ ＜ ０． ００１）． Ａｃｃｏｒｄｉｎｇ ｔｏ ｔｈｅ ｇｏｌｄ ｓｔａｎｄａｒｄ ｏｆ ｈｉｓｔｏｐａｔｈｏｌｏｇｉｃａｌ ｄｉａｇｎｏｓｉｓ， ｔｈｅ ｃｏｉｎｃｉｄｅｎｃｅ ｒａｔｅ ｏｆ ｃｙｔｏｍｏｒｐｈｏｌｏｇｙ ｃｏｍｂｉｎｅｄ ｗｉｔｈ ＩＣＣ ｗａｓ ｈｉｇｈｅｒ ｔｈａｎ ｔｈａｔ ｏｆ ｃｅｌｌ ｍｏｒｐｈｏｌｏｇｙ ａｌｏｎｅ． Ｔｈｅ ｃｏｉｎｃｉｄｅｎｃｅ ｒａｔｅ ｏｆ ｓｑｕａｍｏｕｓ ｃｅｌｌ ｃａｒｃｉｎｏｍａ ｗａｓ ｉｎｃｒｅａｓｅｄ ｆｒｏｍ ８５．２％ ｔｏ ９７．１％ （Ｐ ＝０．０９３）， ａｄｅｎｏｃａｒｃｉｎｏｍａ ｆｒｏｍ ９２．５％ ｔｏ ９８．０％ （Ｐ＜０．００１）， ａｎｄ ｓｍａｌｌ ｃｅｌｌ ｃａｒｃｉｎｏｍａ ｆｒｏｍ ９６．１％ ｔｏ ９８． ３％ （Ｐ ＝ ０． ４６５）． Ｃｏｎｃｌｕｓｉｏｎ 　Ｔｈｅ ｃｏｍｂｉｎａｔｉｏｎ ｏｆ ｌｉｑｕｉｄ⁃ｂａｓｅｄ ｔｈｉｎ⁃ｐｒｅｐ ｃｙｔｏｌｏｇｙ ａｎｄ ａｕｔｏｍａｔｉｃ ｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｓｔｒｙ ｃａｎ ｅｆｆｅｃｔｉｖｅｌｙ ｉｍｐｒｏｖｅ ｔｈｅ ａｃｃｕｒａｃｙ ｏｆ ｐａｔｈｏｌｏｇｉｃａｌ ｔｙｐｉｎｇ ｏｆ ｂｒｕｓｈｉｎｇ ｓｐｅｃｉｍｅｎｓ ｕｎｄｅｒ ｆｉｂｅｒｏｐｔｉｃ ｂｒｏｎｃｈｏｓｃｏｐｙ， ａｎｄ ｐｒｏｖｉｄｅ ｍｏｒｅ ｏｂｊｅｃｔｉｖｅ ｄｉａｇｎｏｓｔｉｃ ｒｅｓｕｌｔｓ ｆｏｒ ｃｌｉｎｉｃａｌ ｔｒｅａｔｍｅｎｔ． [ABSTRACT FROM AUTHOR]

Objective To explore the feasibility of conventional smears and liquid-based cytologic slides of lymphatic metastasis specimens of lung adenocarcinoma acquired by fine needle aspiration cytology (FNAC) to detect the expression of anaplastic lymphoma kinase (ALK/D5F3) by immunocytochemistry (ICC) analysis. Methods The lymphatic metastasis specimens of 147 lung adenocarcinoma，including 100 liquid-based cytologic slides and 47 conventional smears，were collected in this study. ALK fusion protein was detected by Roche Ventana ICC technology，which was compared with the ALK fusion gene assessed by fluorescence in situ hybridization (FISH) or reverse transcriptase-polymerase chain reaction ( RT-PCR). Results The positive rate of ALK ( D5F3) fusion protein in advanced lung adenocarcinoma acquired by FNAC was 11.6% (17/147)，and 10.6% (5/47) and 12.0% (12/100) were reached in conventional smears and liquid-based cytologic slides，respectively. Among 147 cases，57 cases including 17 positive cases and 40 negative cases were verified by RT-PCR and FISH. The whole coincidence rate reached 96.5% (55/ 57). The sensitivity and specificity of ALK ( D5F3) fusion protein detected in lung adenocarcinoma acquired by FNAC were 94.1% (16/17) and 97.5% (39/40)，respectively. The sensitivity and specificity were both 100% (5/5 of sensitivity and 10/10 of specificity) in conventional smears，while 91.7% (11/12) and 96.7% (29/30) in liquid-based cytologic slides. Conclusion Conventional smears and liquid-based cytologic slides of FNAC samples can be used to perform ICC analysis of ALK ( D5F3) expression in advanced lung adenocarcinoma, especially for patients who have no opportunity for surger^^ or whose resected samples are difficult to form cell block. [ABSTRACT FROM AUTHOR]

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