1. Ontogeny and phagocytic function of baboon lung dendritic cells.
- Author
-
Awasthi S, Wolf R, and White G
- Subjects
- Age Factors, Animals, B7-1 Antigen immunology, B7-2 Antigen immunology, CD11c Antigen immunology, CD40 Antigens immunology, Cell Lineage immunology, Cell Separation methods, Cells, Cultured, Centrifugation, Density Gradient, Dendritic Cells cytology, Escherichia coli immunology, Flow Cytometry, Hot Temperature, Immunophenotyping, Lung embryology, Papio anubis embryology, Papio cynocephalus embryology, Dendritic Cells immunology, Lung immunology, Papio anubis immunology, Papio cynocephalus immunology, Phagocytosis immunology
- Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells, but the ontogeny and functions of lung DCs are not known during prenatal period. Here, we isolated lung DC population from fetal (125-175 days of gestation age) and adult baboons. The cells were stained with fluorochrome-conjugated-HLA-DP, DQ, DR, CD1a, CD11c, CD14, CD40, CD80, CD86, CD209, CMKLR1, ILT7-specific antibodies, and staining was analyzed by flow cytometry. The phagocytic function was investigated by incubating the cells with fluorescent-labeled Escherichia coli bioparticles and analyzed by flow cytometry and fluorescence microscopy. The fetal baboon lung DCs expressed low levels of HLA-DP, DQ, DR, CD11c and CD86 as compared to adult baboon lung DCs and showed distinct DC morphology. The fetal lung DCs were also less capable of phagocytosing E. coli as compared to the adult lung DCs (P<0.05). In conclusion, the fetal lung DCs are not only phenotypically immature, but also less efficient in phagocytosing E. coli.
- Published
- 2009
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