1. Pulmonary functional and morphological damage after exposure to tripoli dust.
- Author
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Machado MN, Schmidt AC, Saldiva PH, Faffe DS, and Zin WA
- Subjects
- Acute Disease, Animals, Female, Granuloma, Respiratory Tract etiology, Granuloma, Respiratory Tract pathology, Granuloma, Respiratory Tract physiopathology, Interleukin-1beta metabolism, Interleukin-6 metabolism, Mice, Mice, Inbred BALB C, Pneumonia etiology, Pneumonia pathology, Pneumonia physiopathology, Random Allocation, Silicon Dioxide toxicity, Sodium Chloride administration & dosage, Tumor Necrosis Factor-alpha metabolism, Dust analysis, Inhalation Exposure adverse effects, Lung pathology, Lung physiopathology
- Abstract
Tripoli is a microcrystalline siliceous rock used to polish metals and precious stones. Its inhalation has been associated with increased prevalence of breathing complaints and pneumoconiosis. However, its acute human exposure has not been so far studied. We aimed at evaluating the putative mechanical, morphological, biochemical and inflammatory lung damage in mice acutely exposed to Tripoli dust. BALB/c mice were randomly assigned to 2 groups: In control group (CTRL, n=6) animals received intratracheally (i.t.) 0.9% NaCl (50μl), while Tripoli group (TRIP, n=15) received 20mg of Tripoli powder diluted in 50μL of saline i.t. The experiments were done 15 days later. TRIP mice showed higher pulmonary mechanical impedance, polymorphonuclear cells, TNF-α, IL1-β and IL-6 than CTRL. TRIP presented granulomatous nodules containing collagenous fibers that occupied 35% of the lung tissue area. In conclusion, acute exposure to Tripoli dust triggered important lung damage in mice lungs that if found in human workers could trigger severe illness., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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