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Your search keyword '"Rimma A. Poltavtseva"' showing total 2 results
Start Over Author "Rimma A. Poltavtseva" Topic luminex
2 results on '"Rimma A. Poltavtseva"'

1. Chemokine Homeostasis in Healthy Volunteers and during Pancreatic and Colorectal Tumor Growth in Murine Models

Author

Elena V. Svirshchevskaya, Mariya V. Konovalova, Eugene V. Snezhkov, Rimma A. Poltavtseva, and Sergey B. Akopov

Subjects
chemokines, tumor model, CT26, Pan02, Luminex, Biology (General), QH301-705.5
Abstract

Chemokines are involved in the humoral regulation of body homeostasis. Changes in the blood level of chemokines were found in cancer, atherosclerosis, diabetes, and other systemic diseases. It is essential to distinguish the effects of co-morbid pathologies and cancer on the level of chemokines in the blood. We aimed to analyze, by multiplex cytometry, the levels of chemokines in the blood of healthy young volunteers as well as of intact mice and mice with CT26 colon and Pan02 pancreatic tumors. Two types of chemokines were identified both in human and murine plasmas: homeostatic ones, which were found in high concentrations (>100 pg/mL), and inducible ones, which can be undetectable or determined at very low levels (0–100 pg/mL). There was a high variability in the chemokine levels, both in healthy humans and mice. To analyze chemokine levels during tumor growth, C57BL/6 and BALB/c were inoculated with Pan02 or CT26 tumor cells, accordingly. The tumors significantly differed in the growth and the mortality of mice. However, the blood chemokine levels did not change in tumor-bearing mice until the very late stages. Taken collectively, blood chemokine level is highly variable and reflects in situ homeostasis. Care should be taken when considering chemokines as prognostic parameters or therapeutic targets in cancer.

Published
2022
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2. Silicon–Gold Nanoparticles Affect Wharton’s Jelly Phenotype and Secretome during Tri-Lineage Differentiation

Author

Elena V. Svirshchevskaya, Nina V. Sharonova, Rimma A. Poltavtseva, Mariya V. Konovalova, Anton E. Efimov, Anton A. Popov, Svetlana V. Sizova, Daria O. Solovyeva, Ivan V. Bogdanov, and Vladimir A. Oleinikov

Subjects
Vascular Endothelial Growth Factor A, Silicon, Metal Nanoparticles, CD13 Antigens, Catalysis, Inorganic Chemistry, Animals, Humans, silicon–gold nanoparticles, Wharton’s jelly mesenchymal stem cells, MSC, osteogenic, chondrogenic, adipogenic differentiation, cell phenotype, soluble factors, Luminex, G-CSG, VEGF, Wharton Jelly, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Secretome, Adipogenesis, Organic Chemistry, technology, industry, and agriculture, Cell Differentiation, Mesenchymal Stem Cells, General Medicine, Computer Science Applications, Phenotype, Thy-1 Antigens, Female, Gold, Chondrogenesis
Abstract

Multiple studies have demonstrated that various nanoparticles (NPs) stimulate osteogenic differentiation of mesenchymal stem cells (MSCs) and inhibit adipogenic ones. The mechanisms of these effects are not determined. The aim of this paper was to estimate Wharton’s Jelly MSCs phenotype and humoral factor production during tri-lineage differentiation per se and in the presence of silicon–gold NPs. Silicon (SiNPs), gold (AuNPs), and 10% Au-doped Si nanoparticles (SiAuNPs) were synthesized by laser ablation, characterized, and studied in MSC cultures before and during differentiation. Humoral factor production (n = 41) was analyzed by Luminex technology. NPs were nontoxic, did not induce ROS production, and stimulated G-CSF, GM-CSF, VEGF, CXCL1 (GRO) production in four day MSC cultures. During MSC differentiation, all NPs stimulated CD13 and CD90 expression in osteogenic cultures. MSC differentiation resulted in a decrease in multiple humoral factor production to day 14 of incubation. NPs did not significantly affect the production in chondrogenic cultures and stimulated it in both osteogenic and adipogenic ones. The major difference in the protein production between osteogenic and adipogenic MSC cultures in the presence of NPs was VEGF level, which was unaffected in osteogenic cells and 4–9 times increased in adipogenic ones. The effects of NPs decreased in a row AuNPs > SiAuNPs > SiNPs. Taken collectively, high expression of CD13 and CD90 by MSCs and critical level of VEGF production can, at least, partially explain the stimulatory effect of NPs on MSC osteogenic differentiation.

Published
2022
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