1. Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila.
- Author
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Gospodaryov DV, Strilbytska OM, Semaniuk UV, Perkhulyn NV, Rovenko BM, Yurkevych IS, Barata AG, Dick TP, Lushchak OV, and Jacobs HT
- Subjects
- Animals, Animals, Genetically Modified, Ciona intestinalis genetics, Drosophila melanogaster genetics, Female, Gene Expression Regulation, Male, NADH Dehydrogenase genetics, Sex Factors, Ciona intestinalis enzymology, Drosophila melanogaster drug effects, Drosophila melanogaster enzymology, Drug Resistance genetics, Energy Metabolism genetics, Longevity genetics, NADH Dehydrogenase metabolism, Xenobiotics pharmacology
- Abstract
Mitochondrial alternative NADH dehydrogenase (aNDH) was found to extend lifespan when expressed in the fruit fly. We have found that fruit flies expressing aNDH from Ciona intestinalis (NDX) had 17-71% lifespan prolongation on media with different protein-tocarbohydrate ratios except NDX-expressing males that had 19% shorter lifespan than controls on a high protein diet. NDX-expressing flies were more resistant to organic xenobiotics, 2,4-dichlorophenoxyacetic acid and alloxan, and inorganic toxicant potassium iodate, and partially to sodium molybdate treatments. On the other hand, NDX-expressing flies were more sensitive to catechol and sodium chromate. Enzymatic analysis showed that NDX-expressing males had higher glucose 6-phosphate dehydrogenase activity, whilst both sexes showed increased glutathione S-transferase activity.
- Published
- 2020
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