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1. Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers.

Author

Wei L, Shao WW, Zhou HB, Ping J, Li LM, and Zhang YP

Subjects
Alleles, Animals, China, Genetic Markers genetics, Genetic Variation, Genetics, Population, Linkage Disequilibrium, Polymorphism, Genetic, Restriction Mapping methods, Restriction Mapping veterinary, Lizards genetics, Microsatellite Repeats
Abstract

Twelve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus. We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36). The mean number of observed alleles per locus was 7.3 (range 4 to 13). Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively. One locus (GJ20) showed significant departure from Hardy-Weinberg equilibrium; no linkage disequilibrium was found between any two loci. These informative microsatellite markers will be useful for population genetic analyses of G. japonicus and other species in the genus Gekko.

Published
2015
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2. Rapid microsatellite development in Gekko japonicus using sequenced restriction-site associated DNA markers

Author

Zhou Hb, Ping J, Zhang Yp, Li Lm, L. Wei, and Shao Ww

Subjects
Genetic Markers, China, Linkage disequilibrium, Restriction Mapping, Population, Locus (genetics), Linkage Disequilibrium, Gekko, Genetics, Animals, education, Molecular Biology, Alleles, Genetic diversity, education.field_of_study, Polymorphism, Genetic, biology, Restriction site associated DNA markers, Genetic Variation, Lizards, General Medicine, biology.organism_classification, Genetics, Population, Genetic marker, Microsatellite, Microsatellite Repeats
Abstract

Twelve polymorphic microsatellite loci were isolated in the Japanese gecko, Gekko japonicus. We genotyped one population from Wenzhou, Zhejiang Province, China (N = 36). The mean number of observed alleles per locus was 7.3 (range 4 to 13). Observed and expected heterozygosity values ranged from 0.200 to 0.944 and from 0.395 to 0.797, respectively. One locus (GJ20) showed significant departure from Hardy-Weinberg equilibrium; no linkage disequilibrium was found between any two loci. These informative microsatellite markers will be useful for population genetic analyses of G. japonicus and other species in the genus Gekko.

Published
2015
Full Text
View/download PDF

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