1. Less demand on stem cell marker-positive cancer cells may characterize metastasis of colon cancer.
- Author
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Kaida T, Fujiyama Y, Soeno T, Yokota M, Nakamoto S, Goto T, Watanabe A, Okuno K, Nie Y, Fujino S, Yokota K, Harada H, Tanaka Y, Tanaka T, Yokoi K, Kojo K, Miura H, Yamanashi T, Sato T, Sasaki J, Sangai T, Hiki N, Kumamoto Y, Naitoh T, and Yamashita K
- Subjects
- Humans, Hyaluronan Receptors genetics, Hyaluronan Receptors metabolism, Protein Isoforms genetics, Neoplastic Stem Cells metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, AC133 Antigen genetics, AC133 Antigen metabolism, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Colonic Neoplasms pathology, Liver Neoplasms pathology
- Abstract
Background: CD44 and CD133 are stem cell markers in colorectal cancer (CRC). CD44 has distinctive isoforms with different oncological properties like total CD44 (CD44T) and variant CD44 (CD44V). Clinical significance of such markers remains elusive., Methods: Sixty colon cancer were examined for CD44T/CD44V and CD133 at mRNA level in a quantitative PCR, and clarified for their association with clinicopathological factors., Results: (1) Both CD44T and CD44V showed higher expression in primary colon tumors than in non-cancerous mucosas (p<0.0001), while CD133 was expressed even in non-cancerous mucosa and rather decreased in the tumors (p = 0.048). (2) CD44V expression was significantly associated with CD44T expression (R = 0.62, p<0.0001), while they were not correlated to CD133 at all in the primary tumors. (3) CD44V/CD44T expressions were significantly higher in right colon cancer than in left colon cancer (p = 0.035/p = 0.012, respectively), while CD133 expression were not (p = 0.20). (4) In primary tumors, unexpectedly, CD44V/CD44T/CD133 mRNA expressions were not correlated with aggressive phenotypes, but CD44V/CD44T rather significantly with less aggressive lymph node metastasis/distant metastasis (p = 0.040/p = 0.039, respectively). Moreover, both CD44V and CD133 expressions were significantly decreased in liver metastasis as compared to primary tumors (p = 0.0005 and p = 0.0006, respectively)., Conclusion: Our transcript expression analysis of cancer stem cell markers did not conclude that their expression could represent aggressive phenotypes of primary and metastatic tumors, and rather represented less demand on stem cell marker-positive cancer cells., Competing Interests: Dr. Naitoh reports financial relationship outside the submitted work: grants and personal fees from Chugai pharm, Taiho pharm, Kaken pharm, Daiichi-Sankyo, and Eli Lilly Japan, personal fees from Takeda pharm, Merck, Bayer, and Boehringer Ingelheim. Dr. Hiki reports financial relationship outside the submitted work: grants and personal fees from Abbott Japan, EA pharm, Johnson&Johnson, Otsuka pharm, Otsuka Pharm Factory Inc., Kaken pharm, Covidien Japan Inc., Takeda Pharm, Daiichi Sankyo, Taiho pharm, Tsumura, Terumo, and Miyarisan pharm, grants from Shionogi pharm, Chugai pharm, Hogy Medical, and Yakult Honsha, personal fees from NHK, Pfizer Japan, AstraZeneca, Nihon pharm, Olympus, Novartis pharm, Intuitive Surgical G.K., Ono pharm, Kaigen pharm, QLife, Sumitomo Dainippon pharm, and Nestle Japan. Dr. Kumamoto reports financial relationship outside the submitted work: grants and personal fees from Taiho pharm, Takeda pharm, and Asahi Kasei pharm, grants from Kaken pharm, Eli Lilly Japan, Chugai pharm, Daiichi Sankyo, Covidien Japan Inc., Yakult Honsha, Nihon pharm, personal fees from Viatris pharm, Ono pharm, AMCO, Novartis pharm, Olympus, Japan Blood Products Organization, and Terumo. Dr.Sangai reports financial relationship outside the submitted work: grants and personal fees from Chugai pharm, Taiho pharm, Eisai, and Kyowa Kirin, grants from Takeda pharm, and Nippon Kayaku, personal fees from Pfizer Japan, AstraZeneca, Novartis pharm, Daiichi Sankyo, Eli Lilly Japan, and Maruho. This does not alter our adherence to PLOS ONE policies on sharing data and materials., (Copyright: © 2023 Kaida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2023
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