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2. Racemic LTA4 methyl ester bioconversion into LTC4 methyl ester by various glutathione S-transferases.

Author

Maury G, Ginestar E, Sraïri D, Thaler-Dao H, Dembélé-Duchesne MJ, Lorquin J, and Crastes de Paulet A

Subjects
Animals, Biotransformation, Chromatography, High Pressure Liquid, Female, Humans, Isoenzymes metabolism, Isomerism, Kinetics, Lipoxygenase metabolism, Rats, SRS-A biosynthesis, Substrate Specificity, Arachidonic Acids metabolism, Glutathione Transferase metabolism, Leukotriene A4 analogs & derivatives, Leukotriene C4 analogs & derivatives, Liver enzymology, Placenta enzymology, SRS-A analogs & derivatives
Abstract

It has been shown that various glutathione transferases can synthesize leukotriene C4, or its methyl ester, from glutathione and leukotriene A4. We questioned whether the same enzymes could be used to resolve racemic leukotriene A4 methyl ester (more easily prepared than the optically active enantiomer) and to produce leukotriene C4 methyl ester selectively. We present in this paper a study of the enantioselectivity of some rat liver glutathione transferase isozymes and of the glutathione transferase of human placenta for the leukotriene A4 methyl ester isomers. The rat liver 3-4 glutathione transferase exhibited the highest conversion rate but preferentially converted the (5R, 6R) leukotriene A4 methyl ester. The placental enzyme was fairly selective for the natural (5S, 6S) enantiomer but the rate of conversion was low.

Published
1987

3. Kinetic resolution of racemic leukotriene A4 by mammalian cytosolic epoxide hydrolases.

Author

Maury G, Ginestar E, Lopez-Parrot H, Thaler-Dao H, and Crastes De Paulet A

Subjects
Animals, Chromatography, High Pressure Liquid, Cytosol enzymology, Female, Guinea Pigs, Kinetics, Leukotriene A4, Liver metabolism, Placenta metabolism, Pregnancy, Radioimmunoassay, Rats, Rats, Inbred Strains, Substrate Specificity, Epoxide Hydrolases metabolism, Leukotriene B4 metabolism, Leukotrienes metabolism, Liver enzymology, Placenta enzymology
Abstract

Cytosols of rat and guinea pig liver and of human placenta were screened for their capacity to catalyze the conversion of racemic leukotriene A4 into 5S, 12R-dihydroxy-(Z,E,E,Z)-6,8,10,14-eicosatetraenoic acid (leukotriene B4). The epoxide hydrolase activities showed some specificity for the 5S,6S-oxido-(E,E,Z,Z)-7,9,11,14-eicosatetraenoic acid (LTA4) and produced mixtures of leukotriene B4 and its enantiomer containing up to 78-87% of leukotriene B4.

Published
1989
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4. The 17 hydroxysteroid NAD (P) oxidoreductases from female rabbit liver cytosol: separation and characterization.

Author

Thaler-Dao H, Descomps B, Saintot M, and Crastes de Paulet A

Subjects
Animals, Chromatography, DEAE-Cellulose, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Estradiol metabolism, Female, Hydrogen-Ion Concentration, Hydroxysteroids, Isoenzymes analysis, Kinetics, Liver cytology, Methods, Molecular Weight, NAD, NADP, Oxidoreductases analysis, Rabbits, Spectrophotometry, Ultraviolet, Testosterone metabolism, Cytosol enzymology, Liver enzymology, Oxidoreductases isolation & purification
Published
1972
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