Search

Your search keyword '"Samsom, Roos-Anne"' showing total 3 results
Start Over Author "Samsom, Roos-Anne" Topic liver
3 results on '"Samsom, Roos-Anne"'

1. High level of polarized engraftment of porcine intrahepatic cholangiocyte organoids in decellularized liver scaffolds

Author

Krüger, Melanie, Samsom, Roos-Anne, Oosterhoff, Loes, van Wolferen, Monique, Kooistra, Hans, Geijsen, Niels, Penning, Louis, Kock, Linda M, Sainz-Arnal, Pilar, Baptista, Pedro Miguel, Spee, Bart, Interne geneeskunde GD, CS_Welfare & emerging diseases, CS_STEM, Orthopaedic Biomechanics, Interne geneeskunde GD, CS_Welfare & emerging diseases, and CS_STEM

Subjects
bioengineering, Tissue Engineering, Tissue Scaffolds, Epithelial Cells, swine, Cell Biology, Extracellular Matrix, organoids, swine, cell polarity, tissue scaffolding, Liver, Hepatocytes, livers, Molecular Medicine, Animals, Humans, organoids
Abstract

In Europe alone, each year 5500 people require a life-saving liver transplantation, but 18% die before receiving one due to the shortage of donor organs. Whole organ engineering, utilizing decellularized liver scaffolds repopulated with autologous cells, is an attractive alternative to increase the pool of available organs for transplantation. The development of this technology is hampered by a lack of a suitable large-animal model representative of the human physiology and a reliable and continuous cell source. We have generated porcine intrahepatic cholangiocyte organoids from adult stem cells and demonstrate that these cultures remained stable over multiple passages whilst retaining the ability to differentiate into hepatocyte- and cholangiocyte-like cells. Recellularization onto porcine scaffolds was efficient and the organoids homogeneously differentiated, even showing polarization. Our porcine intrahepatic cholangiocyte system, combined with porcine liver scaffold paves the way for developing whole liver engineering in a relevant large-animal model.

Published
2022

2. Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling.

Author

Krüger, Melanie, Ruppelt, Alicia, Kappler, Benjamin, Van Soest, Elke, Samsom, Roos Anne, Grinwis, Guy C. M., Geijsen, Niels, Helms, J. Bernd, Stijnen, Marco, Kock, Linda M., Rasponi, Marco, Kooistra, Hans S., and Spee, Bart

Subjects
LIVER diseases, FATTY liver, FREE fatty acids, LIVER, SLAUGHTERING
Abstract

Metabolic and toxic liver disorders, such as fatty liver disease (steatosis) and drug-induced liver injury, are highly prevalent and potentially life-threatening. To allow for the study of these disorders from the early stages onward, without using experimental animals, we collected porcine livers in a slaughterhouse and perfused these livers normothermically. With our simplified protocol, the perfused slaughterhouse livers remained viable and functional over five hours of perfusion, as shown by hemodynamics, bile production, indocyanine green clearance, ammonia metabolism, gene expression and histology. As a proof-of-concept to study liver disorders, we show that an infusion of free fatty acids and acetaminophen results in early biochemical signs of liver damage, including reduced functionality. In conclusion, the present platform offers an accessible system to perform research in a functional, relevant large animal model while avoiding using experimental animals. With further improvements to the model, prolonged exposure could make this model a versatile tool for studying liver diseases and potential treatments. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

3. Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories

Author

Bernal, Paulina Nuñez, Bouwmeester, Manon, Madrid-Wolff, Jorge, Falandt, Marc, Florczak, Sammy, Rodriguez, Nuria Ginés, Li, Yang, Größbacher, Gabriel, Samsom, Roos-Anne, van Wolferen, Monique, van der Laan, Luc, Delrot, Paul, Loterie, Damien, Malda, Jos, Moser, Christophe, Spee, Bart, Levato, Riccardo, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, Afd Pharmacology, dES RMSC, CS_STEM, CS_Locomotion, Surgery, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, Afd Pharmacology, dES RMSC, CS_STEM, and CS_Locomotion

Subjects
disease, Tissue Engineering, Tissue Scaffolds, biofabrication, Mechanical Engineering, Bioprinting, Regenerative, spheroids, Hydrogels, bioink, culture, bioresins, Organoids, Materials Science(all), Liver, SDG 3 - Good Health and Well-being, volumetric additive manufacturing, Mechanics of Materials, Printing, Three-Dimensional, Gelatin, Humans, General Materials Science, protein, hydrogels, light-based 3D printing
Abstract

Organ- and tissue-level biological functions are intimately linked to micro- scale cell–cell interactions and to the overarching tissue architecture. Together, biofabrication and organoid technologies offer the unique potential to engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces the volumetric bioprinting of complex organoid-laden constructs, which capture key functions of the human liver. Volumetric bioprinting via optical tomography shapes organoid- laden gelatin hydrogels into complex centimeter-scale 3D structures in under 20 s. Optically tuned bioresins enable refractive index matching of specific intracellular structures, countering the disruptive impact of cell-mediated light scattering on printing resolution. This layerless, nozzle-free technique poses no harmful mechanical stresses on organoids, resulting in superior viability and morphology preservation post-printing. Bioprinted organoids undergo hepatocytic differentiation showing albumin synthesis, liver-specific enzyme activity, and remarkably acquired native-like polarization. Organoids embedded within low stiffness gelatins (

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results