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1. In a study of the metabolism of l-[(35)S]methionine in vivo, the labelled sulphur compounds of rat liver and brain were separated first by ion-exchange chromatography into two fractions containing (i) free sulphur amino acids such as methionine, cystathionine, cyst(e)ine and homocyst(e)ine and (ii) glutathione. 2. Two-dimensional paper chromatography with butan-1-ol-acetic acid or propionic acid-water in the first direction and 80% acetone or acetone-ethyl methyl ketone-water in the second direction was found superior to other solvent systems for separating the sulphur amino acids. 3. At 10min. after injection of [(35)S]methionine only a small part of the (35)S was found combined in free methionine or other free sulphur amino acids. 4. Evidence was obtained of the presence of adenosyl[(35)S]methionine and adenosyl[(35)S]homocysteine in perchloric acid extracts of rat liver and brain. 5. The trans-sulphuration pathway was active in brain as well as in liver.

1. Measurements were made of the rate of incorporation of (14)C from uniformly (14)C-labelled glucose into individual amino acids of rat brain and liver. 2. At 2.5 min. after intravenous injection of uniformly (14)C-labelled glucose, about 30% of the total radioactivity in the brain was present in the five amino acids studied. At 30 min. after subcutaneous injection the distribution of (14)C in amino acids was: in brain, alanine 2%, gamma-aminobutyrate 4%, aspartate 9%, glutamine 9% and glutamate 37% (total 69%); in liver, alanine 3%, aspartate 2.6%, glutamine 5.3% and glutamate 5.2% (total 18%). About 1% of the total radioactivity was in serine and glycine. 3. In both organs the specific radioactivity of alanine was initially higher than that of the other amino acids examined. The specific radioactivity of gamma-aminobutyrate in the brain was about the same as or higher than that of glutamate. 4. Amino acids of the rat brain were separated into ;free' and ;bound' fractions from brain dispersions in saline (or sucrose) media. Definite differences in the specific activities of the ;bound' and ;free' forms were not apparent.

1. The rate of incorporation of (14)C into pyruvate, alpha-oxoglutarate, lactate and glucose of rat tissues was measured after the subcutaneous injection of uniformly labelled glucose. 2. In rat brain the specific radioactivities of lactate and glucose were similar to that of alanine. In liver the specific radioactivity of glucose was considerably higher than that of lactate or alanine. 3. The specific radioactivities of alpha-oxo acids of rat brain were lower than those of corresponding amino acids, alanine and glutamate. These findings have been explained in relation to metabolic compartments in vivo. 4. The approximate estimated rate of glucose utilization in rat brain in vivo is 0.96mumole/g. of brain/min.