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Start Over Author "Siggens KW" Topic listeria monocytogenes
2 results on '"Siggens KW"'

1. Polymerase chain reaction for the detection of Listeria species and Listeria monocytogenes.

Author

Siggens KW

Subjects
Base Sequence, DNA Primers genetics, DNA, Bacterial genetics, DNA, Bacterial isolation & purification, Electrophoresis, Agar Gel, Molecular Sequence Data, Bacteriological Techniques, Listeria genetics, Listeria isolation & purification, Listeria monocytogenes genetics, Listeria monocytogenes isolation & purification, Polymerase Chain Reaction methods
Published
1995
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2. Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction.

Author

Border PM, Howard JJ, Plastow GS, and Siggens KW

Subjects
Base Sequence, Listeria genetics, Listeria monocytogenes genetics, Molecular Sequence Data, DNA, Bacterial analysis, Food Microbiology, Listeria isolation & purification, Listeria monocytogenes isolation & purification, Polymerase Chain Reaction
Abstract

Five oligonucleotide sequences are described that were used as primers in the polymerase chain reaction (PCR) to amplify specific sequences from Listeria DNA. When all five primers were used in combination, three PCR products were possible; a Listeria specific product that occurs with DNA from any Listeria sp., a Listeria monocytogenes specific product that occurs only in the presence of DNA from this organism and a universal product that is found using DNA from any bacterial source. The occurrence of these PCR products was used as a diagnostic test on bacteria isolated from various food samples to detect Listeria sp. and L. monocytogenes.

Published
1990
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