1. Extraction of small molecule from human plasma by prototyping 3D printed sorbent through extruded filament for LC-MS/MS analysis.
- Author
-
Adye, Daya Raju, Ponneganti, Srikanth, Malakar, Tushar Kanti, Radhakrishnanand, Pullapanthula, Murty, Upadhyayula Suryanarayana, Banerjee, Subham, and Borkar, Roshan M.
- Subjects
- *
FUSED deposition modeling , *THREE-dimensional printing , *LIQUID chromatography-mass spectrometry , *SMALL molecules , *FIBERS , *RAPID prototyping , *EXTRACTION techniques - Abstract
Analytical sample preparation techniques are regarded as crucial steps for analyzing compounds from different biological matrices. The development of new extraction techniques is a modern trend in the bioanalytical sciences. 3D printed techniques have emerged as a valuable technology for prototyping devices in customized shapes for a cost-effective way to advance analytical sample preparation techniques. The present study aims to fabricate customized filaments through the hot-melt extrusion (HME) technique followed by fused deposition modeling mediated 3D printing process for rapid prototyping of 3D printed sorbents to extract a sample from human plasma. Thus, we fabricated our own indigenous filament using poly (vinyl alcohol), Eudragit® RSPO, and tri-ethyl citrate through HME to prototype the fabricated filament into a 3D printed sorbent for the extraction of small molecules. The 3D sorbent was applied to extract hydrocortisone from human plasma and analyzed using a validated LC-MS/MS method. The extraction procedure was optimized, and the parameters influencing the sorbent extraction were systematically investigated. The extraction recovery of hydrocortisone was found to be >82% at low, medium, and high quality control samples, with a relative standard deviation of <2%. The intra-and inter-day precisions for hydrocortisone ranged from 1.0% to 12% and 2.0%–10.0%, respectively, whereas the intra-and inter-day accuracy for hydrocortisone ranged from 93.0% to 111.0% and 92.0% to 110.0%, respectively. The newly customizable size and shape of the 3D printed sorbent opens new possibilities for extracting small molecules from human plasma. [Display omitted] • Developed indigenous filaments through hot-melt-extrusion technique followed by 3D printed sorbent. • 3D printed sorbents used for sample extraction of molecule. • The extraction recovery of hydrocortisone was found to be >82% with <2% relative standard deviation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF