1. Potential plasma lipid biomarkers in early-stage breast cancer.
- Author
-
Jiang N, Zhang G, Pan L, Yan C, Zhang L, Weng Y, Wang W, Chen X, and Yang G
- Subjects
- Adult, Aged, Biomarkers, Tumor analysis, Breast Neoplasms blood, Breast Neoplasms metabolism, Case-Control Studies, Chromatography, High Pressure Liquid, Female, Humans, Least-Squares Analysis, Lipids analysis, Middle Aged, Neoplasm Staging, Tandem Mass Spectrometry, Biomarkers, Tumor blood, Breast Neoplasms pathology, Lipids blood
- Abstract
Objective: To find new biomarkers for early diagnosis of breast cancer., Results: 847 lipid species were identified from 78 plasma samples (37 breast cancer samples and 41 healthy controls) by ultra HPLC coupled with quadrupole time-of-flight tandem mass spectrometry. These include 321 glycerophospholipids (GPs), 265 glycerolipids (GLs), 91 sphingolipids (SPs), 77 fatty acyls (FAs), 68 sterol lipids (STs), 18 prenol lipids (PRs), 6 polyketides (PKs), and 1 saccharolipid (SL). Separation was observed from an orthogonal signal correction Partial Least Square Discrimination Analysis model. Based on this analysis, six differentiating lipids were identified: PC (20:2/20:5), PC (22:0/24:1), TG (12:0/14:1), and DG (18:1/18:2) had high levels, whereas PE (15:0/19:1) and N-palmitoyl proline had low levels in the breast cancer samples compared with the healthy controls. Furthermore, significant differences in metabolites were found among some clinical characteristics., Conclusions: Our results reveal that six specific lipids could serve as potential biomarkers for early diagnosis of breast cancer.
- Published
- 2017
- Full Text
- View/download PDF