Search

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats. U našoj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaštitne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naših rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova. Projekat ministarstva br. 143035B

Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment. Specimens of hake (Merluccius merluccius L) and red mullet (Mullus barbatus L) were collected from the locality in front of sea-port Bar (South Adriatic Sea) at the end of May 2005. Activity of the enzymes of the antioxidative system (superoxide dismutase - SOD and catalase - CAT) and parameters of oxidative stress (lipid peroxidation - LP and reduced glutathione - GSH content) were determined in the liver of hake and red mullet. Obtained results showed that the activities of SOD and CAT were significantly lower in the liver of hake than in red mullet. Higher concentrations of LP and GSH in liver of hake were recorded, due to the changes of physical-chemical parameters of environment. The intensity of oxidative stress in liver of hake was higher in comparison to red mullet. Ribe predstavljaju najznačajnije organizme koji se koriste u biomonitoringu vodenih ekosistema. Parametri oksidacionog stresa u tkivima riba predstavljaju značajne biomarkere u proceni stanja životne sredine. Krajem maja 2005. godine uzorkovane su jedinke oslića (Merluccius merluccius L) i trlje (Mullus barbatus L) sa lokaliteta ispred luke Bar (Južni Jadran). Aktivnost enzima antioksidacionog sistema (superoksid dismutaze - SOD i katalaze - CAT) i parametri oksidacionog stresa (koncentracija lipidnih peroksida - LP i redukovanog glutationa - GSH) su određivani u jetri oslića i trlje. Dobijeni rezultati su pokazali da su aktivnosti SOD i CAT značajno manje u jetri oslića nego kod trlje. Zabeležena je veća koncentracija LP i GSH u jetri oslića, kao posledica promena fizičko-hemijskih parametara sredine i prisustva polutanata. Intenzitet oksidacionog stresa u jetri oslića je bio viši u odnosu na trlju. Projekat ministarstva br. 143035B

Fish are the most important organisms used in biomonitoring of aquatic ecosystems. Parameters of oxidative stress in fish tissues represent significant biomarkers in the assessment of the status of environment. Specimens of hake (Merluccius merluccius L.) and sea bream (Pagellus erythrinus L.) were collected at the end of May 2005 from the locality in front of sea-port Bar (South Adriatic Sea). Parameters of oxidative stress (concentration of lipid peroxidase - LP and reduced glutathione - GSH) were determined in the liver and white muscle of hake and sea bream. Physical-chemical parameters (salinity, concentration of dissolved oxygen and temperature) were determined in water of the investigated locality. Obtained results showed that the concentration of LP was higher in liver of hake and sea bream in comparison to white muscle. However, significantly higher concentration of GSH was found in white muscle of hake and sea bream in comparison to liver. These changes of oxidative stress parameters point to tissue specificity, which is the consequence of different metabolic and antioxidative activity. Inter-specific differences were also established. Higher concentrations of LP and GSH in liver of hake and in white muscle of sea bream were recorded, due to the changes of physical-chemical parameters of environment. The intensity of oxidative stress in liver of hake and sea bream was higher in comparison to muscle. Ribe predstavljaju najznačajnije organizme koji se koriste u biomonitoringu vodenih ekosistema. Parametri oksidacionog stresa u tkivima riba predstavljaju značajne biomarkere u proceni stanja životne sredine. Krajem maja 2005. godine uzorkovane su jedinke oslića (Merluccius merluccius L.) i arbuna (Pagellus erythrinus L.) sa lokaliteta ispred luke Bar (Južni Jadran). Parametri oksidacionog stresa (koncentracija lipidnih peroksida - LP i redukovani glutationa - GSH) su određivani u tkivima jetre i belog mišića oslića i arbuna. U vodi ispitivanog lokaliteta određivani su i fizičko-hemijski parametri (salinitet, koncentracija rastvorenog kiseonika i temperatura). Dobijeni rezultati su pokazali da je koncentracija LP veća u jetri oslića i arbuna u odnosu na belo mišićno tkivo. Međutim, zabeležena je znatno veća koncentracija GSH u belom mišiću i oslića i arbuna u odnosu na jetru. Ovakve promene parametara oksidacionog stresa ukazuju na tkivnu specifičnost, kao posledica različite metaboličke i antioksidacione aktivnosti. Ustanovljene su i interspecijske razlike, zabeležena je veća koncentracija LP i GSH u jetri oslića, kao i u belom mišiću arbuna, kao posledica promena fizičko-hemijskih parametara sredine i prisustva polutanata. Intenzitet oksidacionog stresa u jetri oslića i arbuna je bio viši u odnosu na mišiće. null

Cadmium (Cd+2) is an ubiquitous toxic metal that may induce oxidative damage by disturbing the prooxidant-antioxidant balance in the blood. Wistar albino rats (3 months old), were injected with a single dose of CdCh (0.4 mg Cd/kg i.p. and sacrificed after 24h). The hematological parameters: red blood cells count (RBCs), hematocrite (Ht) value and hemoglobin (Hb) concentration were significantly decreased in the blood of Cd treated rats. The activities of enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in plasma, as well as concentration of blood glucose were significantly increased in animals treated with cadmium in comparison to control values. The treatment with Cd increased lipid peroxidation (LP) and reduced glutathione (GSH) contents in the blood, suggesting that the Cd induced oxidative stress. These results of our study suggested that Cd induced alterations in hemato-biochemical parameters and LP and GSH content. Kadmijum (Cd+2) je široko rasprostranjen toksičan metal koji može izazvati oksidaciona oštećenja u krvi remeteći prooksidaciono-antioksidacionu ravnotežu. Wistar albino pacovi (stari 3 meseca), tretirani su pojedinačnim dozama CdCl; (0.4 mg Cd/kg i.p) i žrtvovani posle 24 časa. Hematološki parametri, i to: broj eritrocita (RBCs), hematokritska vredno.st (Ht) i koncentracija hemoglobina (Hb) značajno su smanjeni u krvi tretiranih pacova. Aktivnost enzima alanin aminotransaminaze (ALT) i aspartat aminotransaminaze (AST) u plazmi, kao i koncentracija glukoze u krvi značajno su povećani kod životinja tretiranih kadmijumom u poređenju sa kontrolnim vrednostima. Tretman sa kadmijumom, takođe je doveo do povećanja lipidne peroksidacije (LP) i redukovanog glutationa (GSH). Sve to ukazuje da je kadmijum izazvao oksidacioni stres u krvi pacova. Dobijeni rezultati ukazuju da je Cd indukovao promené u hemato-biohemijskim parametrima, kao i u sadržaju LP i GSH u krvi tretiranih pacova. null

Fish are the most important organisms in the biomonitoring of aquatic ecosystems. A number of pollutants may cause the oxidative stress in the organism of fish. The monitoring of parameters of oxidative stress in tissues of fish is a good biomarker in the assessment of the condition of environment. Catch of commercially important species of fish, hake (Merluccius merluccius L.) was carried out in front of sea-port Bar (South Adriatic) at the end of May 2005. The parameters of oxidative stress: concentrations of lipid peroxides (LP) and reduced glutathione (GSH) were determined in liver and white muscle. Physical-chemical parameters (salinity, concentration of dissolved oxygen and temperature) as well as the concentrations of nitrites, nitrates and detergents in the water of investigated locality were determined. The obtained results showed the tissue specificity that was the consequence of different metabolic and antioxidative activities. In the liver of hake, the increased concentration of LP was found in comparison to the white muscle, whereas in white muscle the increased concentration of GSH was recorded in comparison to the liver. These results suggest that the intensity of oxidative stress was higher in the liver in comparison to the white muscle, which is the consequence of increased presence of pollutants in water of the investigated locality. . Projekat ministarstva br. 143035B