1. The Immobilization of Lipases on Porous Support by Adsorption and Hydrophobic Interaction Method.
- Author
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Mokhtar, Nur Fathiah, Abd. Rahman, Raja Noor Zaliha Raja, Muhd Noor, Noor Dina, Mohd Shariff, Fairolniza, and Mohamad Ali, Mohd Shukuri
- Subjects
LIPASES ,HYDROPHOBIC interactions ,IMMOBILIZED enzymes ,BINDING sites ,THERAPEUTIC immobilization ,ADSORPTION (Chemistry) - Abstract
Four major enzymes commonly used in the market are lipases, proteases, amylases, and cellulases. For instance, in both academic and industrial levels, microbial lipases have been well studied for industrial and biotechnological applications compared to others. Immobilization is done to minimize the cost. The improvement of enzyme properties enables the reusability of enzymes and facilitates enzymes used in a continuous process. Immobilized enzymes are enzymes physically confined in a particularly defined region with retention to their catalytic activities. Immobilized enzymes can be used repeatedly compared to free enzymes, which are unable to catalyze reactions continuously in the system. Immobilization also provides a higher pH value and thermal stability for enzymes toward synthesis. The main parameter influencing the immobilization is the support used to immobilize the enzyme. The support should have a large surface area, high rigidity, suitable shape and particle size, reusability, and resistance to microbial attachment, which will enhance the stability of the enzyme. The diffusion of the substrate in the carrier is more favorable on hydrophobic supports instead of hydrophilic supports. The methods used for enzyme immobilization also play a crucial role in immobilization performance. The combination of immobilization methods will increase the binding force between enzymes and the support, thus reducing the leakage of the enzymes from the support. The adsorption of lipase on a hydrophobic support causes the interfacial activation of lipase during immobilization. The adsorption method also causes less or no change in enzyme conformation, especially on the active site of the enzyme. Thus, this method is the most used in the immobilization process for industrial applications. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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