1. Ultrafast Excitation Energy Transfer Dynamics in the LHCII–CP29–CP24 Subdomain of Plant Photosystem II
- Author
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Thanh Nhut Do, Hoang Long Nguyen, Parveen Akhtar, Kai Zhong, Thomas L. C. Jansen, Jasper Knoester, Stefano Caffarri, Petar H. Lambrev, Howe-Siang Tan, School of Physical and Mathematical Sciences, Theory of Condensed Matter, Zernike Institute for Advanced Materials, Luminy Génétique et Biophysique des Plantes (LGBP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
- Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,01.03. Fizikai tudományok ,Energy Transfer ,Chemistry [Science] ,Electronic Spectrum ,Plant Photosystem ,Light-Harvesting Protein Complexes ,Photosystem II Protein Complex ,General Materials Science ,Plants ,Physical and Theoretical Chemistry ,Thylakoids ,ComputingMilieux_MISCELLANEOUS - Abstract
We measure the two-dimensional electronic spectra of the LHCII(M)-CP29-CP24 complex in photosystem II (PSII) and provide the first study of the ultrafast excitation energy transfer (EET) processes of an asymmetric and native light-harvesting assembly of the antenna of PSII. With comparisons to LHCII, we observe faster energy equilibrations in the intermediate levels of the LHCII(M)-CP29-CP24 complex at 662 and 670 nm. Notably, the putative "bottleneck" states in LHCII exhibit faster effective dynamics in the LHCII(M)-CP24-CP29 complex, with the average lifetime shortening from 2.5 ps in LHCII to 1.2 ps in the bigger assembly. The observations are supported by high-level structure-based calculations, and the accelerated dynamics can be attributed to the structural change of LHCII(M) in the bigger complex. This study shows that the biological functioning structures of the complexes are important to understand the overall EET dynamics of the PSII supercomplex. Ministry of Education (MOE) This work was supported by grants from the Singapore Ministry of Education Academic Research Fund (Tier 1 RG2/19 and Tier 1 RG14/20 to Howe-Siang Tan), the National Research, Development and Innovations Office, Hungary (NKFIH 2018- 1.2.1-NKP-2018-000009 to Petar H. Lambrev), and the Eötvös Loránd Research Network (KÖ -36/2021 to Petar H. Lambrev).
- Published
- 2022