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Predicting load-carrying capacity of timber beams with holes requires a model capable of accounting for the microscopic material behaviour that influences crack initiation and propagation. The complex stress distribution around the periphery of a hole causes additional tension perpendicular to grain stresses, which can change the failure mode of the beam. This situation can also be affected with a change of hole location within the beam depth because stress intensity factor will be increased by tensile stresses and decreased by compressive stresses. This is not an unlikely situation as services often have to pass through beams at different depths. This paper investigates the effect of changing the hole location through the depth of laminated veneer lumber (LVL) beams utilising an experimental and numerical investigation. Experimental tests to failure of LVL beams and numerical simulations using finite element methods show that for a hole eccentricity of less than 20% of the beam depth, the load-carrying capacity of the beam does not change significantly. For uniformly distributed loading, a linearly decreasing stress intensity factor from the support to mid-span is exhibited, showing an increase in load-carrying capacity as the opening approached mid-span. [ABSTRACT FROM AUTHOR]