Turkonalassus mavi M. Nabozhenko & B. Keskin, sp. n. and Odocnemis rufocruralis M. Nabozhenko & B. Keskin, sp. n., two distinctive darkling beetle species of the tribe Helopini, are described from the Southeastern Anatolia region of Turkey, based on both their external morphology and genital structures. Turkonalassus mavi sp. n. differs from all congeners by the bluish dorsal surface of the body, the pronotum widest before middle, and wide merged baculi of the median lobe of the aedeagus. Odocnemis rufocruralis sp. n. belongs to the praelonga species-group. Odocnemis rufocruralis sp. n. is similar to O. torosica Nabozhenko & Keskin, 2016, O. terminassianae (Nabozhenko, 2011) and O. kakunini Nabozhenko & Keskin, 2016 but differs from all three taxa by the reddish-brown body with red legs, male apical maxillary palpomere wider than in female, elevate and narrowly separate male protrochanters, and the structure of the very long and narrow apical piece of the aedeagus. New data on distribution, bionomics, and trophic relations of several species of Helopini from Turkey are given. Host lichens are determined for nine species. The majority of studied adult beetles feed on lichens from the families Physciaceae and Parmeliaceae. Some examined taxa feed on Cladoniaceae. Feeding on crustose lichens is registered for the first time for Coleoptera, specifically Odocnemis rufocruralis sp. n. was observed to feed on Pertusaria sp. (on Prunus) in Mardin Province., Russian Foundation for Basic Research; RPF [19-54-25001]; TUBITAK 1001 Project [119Z102]; basic research project of Precaspian Institute of Biological Resources of the Daghestan Federal Research Centre of the Russian Academy of Sciences [AAAA-A17-117081640018-5], The study was funded by the Russian Foundation for Basic Research and RPF (project No. 19-54-25001) and TUBITAK 1001 Project No 119Z102, and partly by the basic research project of Precaspian Institute of Biological Resources of the Daghestan Federal Research Centre of the Russian Academy of Sciences, registration number AAAA-A17-117081640018-5. Authors thank Dr Wolfgang Schawaller (SMNS) for loan material, Konstantinos Ntatsopoulos and Loudmila Jelinscaia Lagou (University of Cyprus) for information about habitats and host lichens of Catomus hesperides in Cyprus. We also thank two reviewers, Marcin Jan Kamiski (Purdue University, West Lafaette, USA and Museum and Institute of Zoology of the Polish Academy of Sciences, Warszawa, Poland) and Harold Labrique (Musee des Confluences, Lyon, France) for valuable comments and corrections.