25 results on '"Boyse EA"'
Search Results
2. New mutant and congenic mouse stocks expressing the murine leukemia virus-associated thymocyte surface antigen GIX.
- Author
-
Stockert E, Boyse EA, Obata Y, Ikeda H, Sarkar NH, and Hoffman HA
- Subjects
- Animals, Cell Membrane immunology, Mice, Serotyping, Antigens, Leukemia Virus, Murine, Mice, Inbred Strains, Mutation, T-Lymphocytes immunology
- Abstract
For several reasons the G(IX) antigen (1) has a prominent place in current work on murine leukemia virus (MuLV): In the prototype G(IX+) mouse strain 129, the G(IX) trait is mendelian, and is expressed selectively (though not exclusively) on thymocytes. Thus, expression of this cell surface component is under the control of cellular genes and is subject to the controls governing the differentiation of T lymphocytes (2). Although the 129 mouse produces no demonstrable leukemia virus such as that found in the AKR strain, it was soon realized that G(IX) antigen must in some way be related to MuLV, because productive infection with MuLV is frequently associated with appearance of G(IX) antigen on cells that are genotypically G(IX-), most notably on MuLV-infected rat cells, or cells that belong to other differentiation pathways (1). The basis of this connection between G(IX) and MuLV has recently become clear from the demonstration that G(IX) is one of MuLV envelope. Therefore, our working hypothesis is that the presence of G(IX) is one of the antigens present on gp69/71 (3,4), the major glycoprotein component of the MuLV envelope. Therefore, our working hypothesis is that the presence of G(IX) antigen always denotes the presence of gp69/71 (though not all variants of gp69/71 need necessarily carry G(IX)). Study of the circumstances under which G(IX) is expressed on the cell surface is thus potentially a powerful approach to understanding how the expression of C-type viral genomes is controlled. Such studies are greatly facilitated by the availability of mutant and congenic strains of inbred mice which differ from the nonmutant or partner strains only with respect to one or another manifestation of the viral genome. It is for this reason that we record here (Table I) some details of two G(IX) mutant and two G(IX) congenic stocks derived in our colonies at Memorial Sloan-Kettering Cancer Center (MSKCC). In addition, to these four strains, Table I includes data for the three relevant partner strains, and for strain AKR, for comparison. These eight strains all differ from one another with respect to one or more MuLV-related traits. more...
- Published
- 1975
- Full Text
- View/download PDF
Catalog
3. Spontaneous autoimmunization to GIX cell surface antigen in hybrid mice.
- Author
-
Obata Y, Stockert E, Boyse EA, Tung JS, and Litman GW
- Subjects
- Animals, Antibodies, Viral biosynthesis, Antibody Formation, Antibody Specificity, Genes, Dominant, Glycoproteins immunology, Hybridization, Genetic, Immunoglobulin M biosynthesis, Mice, Mice, Inbred Strains, Antigens, Viral, Autoantibodies, Leukemia Virus, Murine immunology, T-Lymphocytes immunology
- Abstract
The GIX antigen expressed on the thymocytes of GIX+ mice is a type-specific constituent of glycoprotein gp70, which forms the major envelope component of murine leukemia virus. In the prototype GIX+ mouse strain 129, this glycoprotein is a Mendelian character expressed independently of virus production. In the intact thymocyte plasma membrane, part of this glycoprotein, bearing group-specific (gs) antigen, is inaccessible to antibody. The moiety bearing the type-specific GIX determinant is accessible to GIX antibody, which may be an important factor in determining the consequences of autoimmune responses involving GIX. Previously, all attempts to induce GIX antibody in mice had failed. We now find that the hybrid mouse (B6-GIX+ X 129) spontaneously produces substantial amounts of GIX antibody, presumably of the IgM class appearing as early as 2 mo of age. The specificity of the GIX natural mouse antibody is the same as that recognized by the conventional GIX typing serum produced in rats ("anti-NTD"). As neither parent strain produces appreciable GIX antibody, we surmise that this autoimmune response requires two dominant genes, each parent contributing a high-response allele to the hybrid. These can be envisaged as two immune response loci, controlling different immunocompetent cells which must cooperate to produce GIX antibody. Production of GIX antibody by the hybrids increases progressively with age. This is accompanied by decreased expression of GIX antigen on their thymocytes. We attribute this to antigenic modulation. Antibody to gs antigen of gp70 is also found in autoimmune (B6-GIX+ X 129) hybrids but not in either parent strain. We are investigating evidence of a pathological autoimmune syndrome in these hybrids. The special interest of this syndrome is that it presumably signifies the consequences of autoimmunization to a single C-type virus component, expressed without significant virus production, in a mouse with no evident genetic predisposition to such disease in the absence of that antigen. more...
- Published
- 1976
- Full Text
- View/download PDF
4. Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus.
- Author
-
Tung JS, Vitetta ES, Fleissner E, and Boyse EA
- Subjects
- Animals, Cell Membrane immunology, Chemical Precipitation, Electrophoresis, Polyacrylamide Gel, Genotype, Glucosamine, Glycoproteins isolation & purification, Immunoglobulin G, Immunologic Techniques, Mice, Molecular Biology, Molecular Weight, Antigens, Viral analysis, Glycoproteins immunology, Isoantigens analysis, Leukemia Virus, Murine immunology, T-Lymphocytes immunology, Viral Proteins analysis
- Abstract
It is known that the thymocyte surface antigen GIX is found in some strains of mice and not others, and that its expression in mice of strain 129, in which most extensive genetic studies have been made, is controlled by two unlinked cellular chromosomal loci. We have now isolated a protein with a mol wt of approximately 70,000 daltons from the surface of thymocytes from 129 mice, which have antigenic and biochemical properties characteristic of the gp69/71 envelope component of murine leukemia virus. Our evidence is compatible with the conclusion that it carries the GIX antigen. more...
- Published
- 1975
- Full Text
- View/download PDF
5. Influence of Fv-1 alleles on cellular expression of gp70.
- Author
-
Tung JS, Boyse EA, and Shen FW
- Subjects
- Animals, Genes, Regulator, Glycoproteins genetics, Membrane Proteins genetics, Mice, Mice, Inbred AKR microbiology, Phenotype, Retroviridae genetics, Virus Replication, Leukemia Virus, Murine genetics, Viral Proteins genetics
- Abstract
Type-variants of gp70 (glycoprotein-70), which is the major envelope protein of C-type mouse virus and is also found in plasma membranes, are identified immunogenetically by the antigens Gix and Ec. Cellular expression of Gix+ gp70 does not depend on production of virus, but expression of Ec+ gp70 (formerly X-gp70) has been observed only in AKR and other strains of mice that produce large amounts of virus throughout life. To test the inference that cellular expression of Ec+ gp70 is secondary to production of virus we examined the effect of Fv-1 alleles, which govern the replicability of N-tropic and B-tropic C-type virus, on the expression of Ec+ gp70 on thymocytes. By typing thymocytes of Fv-1-congenic mice for Ec+ gp70 was found that manifestation of the Ec+ gp70 phenotype requires the Fv-1n allele, which is permissive for replication of N-tropic virus produced by AKR and other virus-producing mouse strains. Substitution of the Fv-1b allele for the Fv-1n allele abolishes demonstrable expression of Ec+ gp70 by AKR thymocytes at ages up to 9 mo, the oldest AKR mice tested. more...
- Published
- 1980
- Full Text
- View/download PDF
6. Heredity of the GIX thymocyte antigen associated with murine leukemia virus: segregation data simulating genetic linkage.
- Author
-
Stockert E, Boyse EA, Sato H, and Itakura K
- Subjects
- Animals, Antigens, Viral, Chromosome Mapping, Genes, Glucosephosphate Dehydrogenase, Histocompatibility Antigens, Mice, Mice, Inbred Strains, Antigens, Genetic Linkage, Leukemia Virus, Murine immunology, T-Lymphocytes immunology
- Abstract
The GIX antigen is a feature of the gp70 envelope glycoprotein of murine luekemia virus (MuLV). This GIX-gp70 molecule is found on the thymocytes of some (GIX+) strains of mice, where its expression is controlled by two mendelian genes, Gv-1 and Gv-2. Previous recombination data involving the prototype GIX+ strain 129 indicated that the H-2 (chromosome 17) and Gv-1 loci are linked, at a distance of 36 units from one another. New data indicate that the association of H-2 and GIX phenotypes is an example of quasi-linkage, evidently dependent in this instance on heterozygosity at a locus or loci in the vicinity of H-2. Other previous recombination data, involving the GIX+ strain AKR, had indicated that the Gpd-1 (chromosome 4) and Gv-1 loci are linked at a distance of 19 units from one another. New data from other crosses show that this association of Gpd-1 (glucose-6-phosphate dehydrogenase 1) and GIX phenotypes also constitutes quasi-linkage, evidently due to heterozygosity at the Fv-1 locus. An important theoretical consequence of quasi-linkage in general is that it should enhance the heritability of particular constellations of unlinked genes, and so influence population structure. Our new data are discussed from the viewpoint that MuLV genomes are apparently concerned in quasi-linkage, and therefore by the same arguments may influence the genetic structure of populations. This in turn may strengthen the view that integrated MuLV genomes are not simply intruded into a self-sufficient cellular genome, but are themselves elements of the cellular genome with primary functions, perhaps in reproduction or embryogenesis. more...
- Published
- 1976
- Full Text
- View/download PDF
7. Relationships of gp70 of MuLV envelopes to gp70 components of mouse lymphocyte plasma membranes.
- Author
-
Tung JS, O'Donnell PV, Fleissner E, and Boyse EA
- Subjects
- Animals, Cell Membrane analysis, Glycoproteins analysis, Mice, Peptide Fragments analysis, Leukemia Virus, Murine analysis, Leukemia, Experimental analysis, Lymphocytes analysis, Membrane Proteins analysis, Viral Proteins analysis
- Abstract
The family of glycoproteins called gp70 includes molecules that are the main constituent of murine C-type viral envelopes, and some that are expressed as mendelian constituents of thymocyte plasma membranes in the absence of virions. To investigate further the relation of viral gp70s to plasma- membrane gp70s we compared peptide maps of gp70s derived by immunoprecipitation from cells infected with chosen viruses and from various thymocytes and leukemiacells known to express one or more of three immunogenetically defined gp70 types: Glx-gp70, X-gp70, and O-gp70. Maps of gp70 from cultured cells infected with ecotropic and xenotropic viruses were distinguishable from one another, and in general resembled gp70 maps prepared directly from ecotropic and xenotropic virions respectively. Maps of gp70s immunoprecipitated from thymocytes of five mouse strains and from two A strain T-cell leukemias also fell into two distinguishable and generally corresponding patterns. Thus peptide-mapping substantiates earlier conclusions that viral gp70s and plasma-membrane gp70s inherited independently of virus-production are highly related or identical molecules. The gp70 maps of thymocytes from B6, B6-G(+IX), 129, and A mice formed a group resembling the map from cultured cells infected with xenotropic virus. Thymocytes from AKR mice, and the two A strain leukemias, gave gp70 maps conforming more to the second pattern, that of cultured cells infected with ecotropic virus. This second pattern probably comprises at least two gp70 types, one of which is X-gp70. Our data indicate that the G(IX)-gp70 and O-gp70 sub-species of gp70 expressed in the cell populations we have studied are coded by xenotropic viral genomes, and X-gp70 by ecotropic viral genomes. more...
- Published
- 1978
- Full Text
- View/download PDF
8. Relation of gp70 to spontaneous cytolytic activity of mouse spleen cells.
- Author
-
Hatzfeld A, Pinter A, Koo GC, and Boyse EA
- Subjects
- Animals, Binding, Competitive, Female, Humans, Leukemia, Experimental immunology, Male, Mice, Mice, Inbred BALB C, Spleen immunology, Time Factors, AKR murine leukemia virus immunology, Cytotoxicity, Immunologic, Leukemia Virus, Murine immunology, Rauscher Virus immunology, Viral Proteins immunology
- Abstract
In comparing spleen cells of inbred and congenic mice for spontaneous capacity to lyse cells of the BALB/c leukemia RLmale1 in vitro, we found that the activity of 129 spleen cells was more than double that of 129-Gix- spleen cells. The only known difference between these two strains is that 129-Gix- mice express no known demonstrable gp70 or p30, whereas 129 mice express both these MuLV-related components as mendelian traits not associated with the production of virions. We infer that MuLV-related components at the cell surface are concerned in effector-target interactions leading to cytolysis under the conditions described. Although the congenic strains B6 (Gix-) and B6-Gix+ differ likewise in expression of the type-variant Gix-gp70, both strains express a second type-variant of gp70. The lytic activity of spleen cells of these two strains for RLmale1 cells was equally high, suggesting that involvement in lytic effector-target interactions is common to gp70 molecules in general. When used as targets rather than as effectors 129 spleen cells were more sensitive to lysis than 129-Gix- spleen cells. Pre-exposure to gp70, purified from R-MuLV, rendered splenic effector cells less lytic. Pre-exposure to gp70 also rendered RLmale1 target cells less sensitive to lysis. One explanation of these findings is that both target cells and effector cells express gp70 and also receptors for gp70 and that this is the basis of mutual cellular recognition leading to lysis in the circumstances described. more...
- Published
- 1981
- Full Text
- View/download PDF
9. Immunoselective loss of parental h antigens by somatic reduction in an H-2 a/H-2 b hybrid mouse leukemia.
- Author
-
Hauschka TS, Hitt SA, Zumpft M, Shows TB, and Boyse EA
- Subjects
- Animals, Antibodies, Neoplasm analysis, Antigens, Neoplasm analysis, Electrophoresis, Starch Gel, Female, Genotype, Glucose-6-Phosphate Isomerase analysis, Histocytochemistry, Karyotyping, Mice, Mice, Inbred C57BL, Neoplasms, Radiation-Induced enzymology, Leukemia Virus, Murine immunology, Neoplasms, Radiation-Induced immunology
- Published
- 1975
10. Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus.
- Author
-
Obata Y, Ikeda H, Stockert E, and Boyse EA
- Subjects
- Absorption, Animals, Antibodies, Antibodies, Viral analysis, Cell Membrane immunology, Complement System Proteins, Cytotoxicity Tests, Immunologic, Epitopes, Fluorescent Antibody Technique, Genotype, Immune Sera, Mice, Mice, Inbred Strains, Molecular Biology, Phenotype, Antigens, Viral analysis, Glycoproteins immunology, Isoantigens analysis, Leukemia Virus, Murine immunology, T-Lymphocytes immunology, Viral Proteins analysis
- Abstract
Expression of Gix surface antigen on thymocytes is an inherited mendelian train of certain strains of mice. We report here the following new findings: (a) Gix antigen was found free in the serum of Gix+ mouse strains. (b) Expression vs. nonexpression of Gix antigen was invariably correlated with presence or absence of the group-specific antigen of Murine leukemia virus (MuLV) gp69/71 in the serum of mice of inbred and segregating populations. (c) Gix antigen could be removed from normal Gix+ mouse serum by precipitation with antiserum to MuLV gp 69/71. (d) Anti-gp69/71 serum was weakly cytotoxic for Gix+ thymocytes, and partially blocked the cytotoxic activity of Gix antibody for Gix+ thymocytes. (e) Purified AKR virus absorbed Gix activity, and disruption of the virions did not increase their absorbing capacity. These serological data indicate that Gix antigen is a constituent of gp69/71, the glycoprotein which is the major component of the MuLV envelope. On present evidence, Gix antigen is represented in intact virions and is probably accessible to Gix antibody. more...
- Published
- 1975
- Full Text
- View/download PDF
11. Biological expression of antigenic determinants of murine leukemia virus proteins gp69-71 and p30.
- Author
-
Ikeda H, Pincus T, Yoshiki T, Strand M, August JT, Boyse EA, and Mellors RC
- Subjects
- AKR murine leukemia virus immunology, Animals, Cell Line, Cell Membrane immunology, Ethyl Ethers, Fibroblasts, Fluorescent Antibody Technique, Glycoproteins immunology, Guanidines, Mice, Moloney murine leukemia virus immunology, Neutralization Tests, Rats, Rauscher Virus immunology, Antigens, Viral, Epitopes, Leukemia Virus, Murine immunology, Retroviridae immunology, Viral Proteins immunology
- Abstract
Antisera to purified structural proteins of Rauscher murine leukemia virus, the major envelope glycoprotein, gp69/71, and the major internal protein, p30, were studied by immunofluorescence of viable and fixed virus-infected cells and by virus neutralization. Group-specific and type-specific determinants of gp69/71 were demonstrated by immunofluorescence and virus neutralization tests, indicating that these determinants are located in the cytoplasm and probably on the cell surface as well as on virus envelope. Antisera against p30 showed anti-group and anti-interspecies activities by immunofluorescence with no virus-neutralizing activity. Both antigenic determinants of gp69/71 were sensitive to guanidine-hydrochloride and to a lesser degree to ether treatment, whereas the group-specific determinants of p30 were relatively stable to these treatments. more...
- Published
- 1974
- Full Text
- View/download PDF
12. X-gp70: a third molecular species of the envelope protein gp70 of murine leukemia virus, expressed on mouse lymphoid cells.
- Author
-
Tung JS, Shen FW, Fleissner E, and Boyse EA
- Subjects
- Animals, Antigens, Neoplasm analysis, Epitopes, Genes, Glycoproteins immunology, Leukemia, Experimental immunology, Mice, Mice, Inbred A immunology, Retroviridae immunology, Leukemia Virus, Murine immunology, T-Lymphocytes immunology, Viral Proteins immunology
- Abstract
Three variants of the gp70 envelope component of MuLV are now recognizable serologically: GIX-gp70, 0-gp70, and X-gp70. The last of these, X-gp70, has so far been found only in mice or cells producing abundant C-type virus. This distinguishes X-gp70, provisionally, from the GIX-gp70 and 0-gp70 variants, each of which can be expressed on normal thymocytes without accompanying virus production, as exemplified by mouse strains 129 and B6, respectively. The X-gp70 genotype, however, is not limited to strains of mice-producing abundant virus, because X-gp70+ leukemias occur in strains of mice which do not produce a great deal of virus and whose thymocytes and other tissues are X-gp70-; this is analogous to the appearance of GIX+ leukemias in GIX- mouse strains. more...
- Published
- 1976
- Full Text
- View/download PDF
13. Expression of the envelope protein gp70 of murine leukemia virus on normal and leukemic lymphoid cells of mice.
- Author
-
Fleissner E, Tung JS, Shen FW, and Boyse EA
- Subjects
- Animals, Cell Membrane immunology, Cell Membrane metabolism, Epitopes, Genes, Leukemia, Experimental genetics, Mice, T-Lymphocytes immunology, T-Lymphocytes metabolism, Antigens, Viral, Glycoproteins metabolism, Leukemia Virus, Murine, Leukemia, Experimental metabolism, Viral Proteins metabolism
- Published
- 1975
- Full Text
- View/download PDF
14. Expression of murine leukemia virus envelope glycoprotein gp69/71 on mouse thymocytes. Evidence for two structural variants distinguished by presence vs. absence of GIX antigen.
- Author
-
Tung JS, Fleissner E, Vitetta ES, and Boyse EA
- Subjects
- Alleles, Animals, Cell Membrane immunology, Chromosome Mapping, Glucosamine metabolism, Immunoelectrophoresis, Mice, Mice, Inbred Strains, Tritium, Viral Proteins, Glycoproteins immunology, Isoantigens analysis, Leukemia Virus, Murine immunology, T-Lymphocytes immunology
- Abstract
Thymocytes of several mouse strains were tested for expression of the gp69/71 envelope component of murine leukemia virus by surface iodination, followed by immunoprecipitation and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Theses strains included two congenic lines differing from their partner stocks with respect to expression of GIX antigen demonstrable in the cytoxicity assay. We conclude that:(a) two structural variants of gp69/71 can be expressed on mouse thymocytes, (b) these are distinguishable by a small difference in mobility in SDS gels, (c) one carries GIX antigen and the other not, (d) they are coded, or their expression is regulated, by different chromosomal loci that are not closely linked, and (e) both can be expressed together on the thymocytes of inbred mice. In the intact thymocyte plasma membrane, the sites of group-specific antigen shared by the two gp69/71 variants, unlike the GIX type specificity carried by only one of them, are probably inaccessible to antibody. more...
- Published
- 1975
- Full Text
- View/download PDF
15. Relationship of infectious murine leukemia virus and virus-related antigens in genetic crosses between AKR and the Fv-1 compatible strain C57L.
- Author
-
Ikeda H, Rowe WP, Boyse EA, Stockert E, Sato H, and Jacobs S
- Subjects
- Animals, Chromosome Mapping, Crosses, Genetic, Female, Genes, Genetic Linkage, Leukemia, Experimental immunology, Male, Mice, Mice, Inbred AKR, Mice, Inbred Strains, Phenotype, Spleen immunology, T-Lymphocytes immunology, Thymus Gland immunology, Antigens, Viral, Leukemia Virus, Murine immunology, Leukemia, Experimental genetics
- Abstract
In a further genetic study of murine leukemia virus (MuLV) and its components we examined the backcross C57L X (C57L X AKR). This population was selected because strains AKR and C57L are both Fv-1n, and the restriction which the Fu-1b allele imposes on the output of virus was thereby obviated. The segregants were scored for three characters: (a) infectious Gross-AKR-type MuLV (V), in the tail; (b) group-specific antigen indicative of p30 internal viral protein, in spleen; and (c) GIX antigen, now thought to be indicative of gp69/71 viral envelope glycoprotein, on thymocytes. Our conclusions are: (a) It is confirmed that the AKR mouse has two unlinked chromosomal genes, Akv-1 and Akv-2, each of which can independently give rise to the life-long high output of MuLV that is characteristic of AKR mice. (b) Of the eight phenotypes that could possibly be derived from segregation of the three pairs of independent alternative traits, seven were observed, but on progeny testing only three were shown to reflect stably heritable genotypes; these were V+p30+GIX+ and V-p30-GIX- (the parental types) and V-p30+GIX+. A third, newly identified AKR gene, designated Akvp, segregating independently of Akv-1 and Akv-2, also determines expression of p30 and GIX but in this case independently of XC-detectable MuLV. (c) The four remaining observed phenotypes, which did not breed true on progeny testing, involved mostly antigen-negative parents yielding antigen-positive progeny; it is likely that these discrepancies represented suppression of phenotype by a maternal resistance factor. more...
- Published
- 1976
- Full Text
- View/download PDF
16. OCCURRENCE OF SOLUBLE ANTIGEN IN THE PLASMA OF MICE WITH VIRUS-INDUCED LEUKEMIA.
- Author
-
STUECK B, OLD LJ, and BOYSE EA
- Subjects
- Animals, Mice, Antibody Formation, Antigens, Blood, Leukemia, Leukemia Virus, Murine, Leukemia, Experimental, Neoplasms immunology, Oncogenic Viruses, Research
- Published
- 1964
- Full Text
- View/download PDF
17. ANTIGENIC CONVERSION OF ESTABLISHED LEUKEMIAS BY AN UNRELATED LEUKAEMOGENIC VIRUS.
- Author
-
STUECK B, OLD LJ, and BOYSE EA
- Subjects
- Animals, Mice, Antigen-Antibody Reactions, Antigens, Immune Sera, Leukemia, Leukemia Virus, Murine, Leukemia, Experimental, Neoplasms, Oncogenic Viruses, Pharmacology, Research, Retroviridae, Sarcoma, Sarcoma, Experimental, Urethane
- Published
- 1964
- Full Text
- View/download PDF
18. [Leukemia-specific antigens in mice--its relation to viral antigens].
- Author
-
Aoki T, Old LJ, and Boyse EA
- Subjects
- Animals, Mice, Antigens, Leukemia immunology, Leukemia Virus, Murine immunology
- Published
- 1966
19. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses.
- Author
-
Geering G, Old LJ, and Boyse EA
- Subjects
- Animals, Mice, Rats, Antigens, Leukemia Virus, Murine, Leukemia, Experimental
- Abstract
Leukemias can be induced in W/Fu inbred rats by neonatal inoculation of normal thymus cells of C58 mice. These leukemias are not transplantable to C58 mice or to adult W/Fu rats, but they can be kept in passage in W/Fu rats aged 0 to 7 days. Adult W/Fu rats inoculated repeatedly with these isogenic leukemias produce cytotoxic and precipitating antibodies. These antisera are of particular value in the analysis of the antigens of leukemia cells and of leukemia viruses because their mode of preparation precludes the formation of antibody against any normal constituents of the cell. Analysis based on the cytotoxic test indicates the presence of 2 distinct cell surface antigens in leukemias induced by Passage A Gross virus or occurring spontaneously in mice of high-incidence strains. All leukemias and other tissues known to contain G (Gross) leukemia antigen have both determinants, but certain leukemias of low-incidence strains have only 1 of them and so were previously classified G-. Immunoprecipitation with these antisera reveals the presence of a cellular antigen common to G+ cells and absent from G- cells; the same antigen can be demonstrated in ether-treated Gross virus, but not in intact virus. This antigen is present also in ether-treated preparations of the Friend, Moloney, and Rauscher leukemia viruses, but not in Bittner (mammary tumor) virus. Thus it may be regarded as a group-specific antigen of murine leukemia viruses, in contrast to the type-specific cellular antigens demonstrable by the cytotoxic test. Four additional antigens associated with leukemias induced by wild-type Gross virus have been demonstrated by immunoprecipitation, but their relation to viral and cellular antigens has not been determined. more...
- Published
- 1966
- Full Text
- View/download PDF
20. FORMATION OF CYTOXIC ANTIBODY AGAINST LEUKEMIAS INDUCED BY FRIEND VIRUS.
- Author
-
OLD L, BOYSE EA, and LILLY F
- Subjects
- Animals, Mice, Antibodies, Antibody Formation, Antigen-Antibody Reactions, Friend murine leukemia virus, Leukemia, Leukemia Virus, Murine, Leukemia, Experimental, Neoplasms immunology, Oncogenic Viruses, Research, Spleen
- Published
- 1963
21. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS.
- Author
-
OLD LJ, BOYSE EA, and STOCKERT E
- Subjects
- Animals, Mice, Antigens, Immune Sera, Leukemia, Leukemia Virus, Murine, Leukemia, Experimental, Leukemia, Lymphoid, Neoplasms immunology, Neoplasms, Experimental, Research, Splenomegaly
- Published
- 1964
- Full Text
- View/download PDF
22. Increased anti-leukaemia activity of E. coli asparaginase in mice infected with L.D.H.-elevating virus.
- Author
-
Old LJ, Iritani C, Stockert E, Boyse EA, and Campbell HA
- Subjects
- Animals, Asparaginase metabolism, Female, Mice, Asparaginase therapeutic use, Leukemia Virus, Murine drug effects
- Published
- 1968
- Full Text
- View/download PDF
23. The G (Gross) leukemia antigen.
- Author
-
Old LJ, Boyse EA, and Stockert E
- Subjects
- Animals, In Vitro Techniques, Leukemia, Lymphoid immunology, Mice, Neoplasm Transplantation immunology, Sarcoma, Experimental immunology, Spleen, Thymus Gland, Antigens, Leukemia Virus, Murine immunology, Leukemia, Experimental immunology
- Published
- 1965
24. Relation of chromosome 4 (linkage group 8) to murine leukemia virus-associated antigens of AKR mice.
- Author
-
Ikeda H, Stockert E, Rowe WP, Boyse EA, Lilly F, Sato H, Jacobs S, and Old LJ
- Subjects
- Animals, Crosses, Genetic, Genetic Linkage, Mice, Mice, Inbred AKR, Phenotype, Antigens, Viral, Chromosomes, Leukemia Virus, Murine
- Abstract
Genes specifying or controlling the expression of G(IX) (cell surface), GCSA (cell surface), and gs (internal viral) antigens are located in chromosome 4 (linkage group [LG] VIII) of the AKR mouse. All three antigens may exhibit mendelian inheritance, mice being antigen positive or antigen negative, but each may also appear in leukemic cells of mice whose inherited genotype was antigen negative. The G(IX)-determining gene in LG VIII of AKR mice apparently is equivalent to Gv-1, which determines expression of the same antigen in 129 strain mice, but which in the latter strain is located in LG IX. As the estimated distance of Gv-1 from H-2 in 129 mice is considerable (37 units) further tests are now indicated to assess the possibility of pseudolinkage in this case. The Fv-1 locus, also located in LG VIII, influences the mouse's titer of MuLV, and might thereby be thought to regulate the G(IX) and gs phenotypes of AKR backcross segregants. But the data indicate a discrete LG VIII locus for G(IX), since expression of this antigen is mendelian and independent of infectious virus titer. Since the G(IX) and GCSA phenotypes of AKR backcross segregants were invariably concordant, these two antigens must be specified or controlled by closely linked genes, and the latter also is presumably independent of virus titer. The question as to what extent expression of gs antigen in the segregants is secondary to virus production is undecided. more...
- Published
- 1973
- Full Text
- View/download PDF
25. Serological analysis of leukemia antigens of the mouse.
- Author
-
Aoki T, Old LJ, and Boyse EA
- Subjects
- Animals, Antigens analysis, Leukemia Virus, Murine, Leukemia, Experimental immunology
- Published
- 1966
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.