1. Functional expression of a locust tyramine receptor in murine erythroleukaemia cells.
- Author
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Poels, J., Suner, M.-M., Needham, M., Torfs, H., De Rijck, J., De Loof, A., Dunbar, S.J., and Broeck, J. Vanden
- Subjects
TYRAMINE ,LEUKEMIA ,MIGRATORY locust - Abstract
The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the Locusta migratoria tyramine receptor (Tyr Loc), an insect G protein-coupled receptor. Functional agonist-dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL-Tyr Loc). Tyramine elicited a dose-dependent increase of cytosolic Ca[sup2+] -ions and an attenuation of forskolin-induced cyclic adenosine monophosphate (AMP) pro-duction. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR / MEL expression system in functional assays for G protein-coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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