Search

Your search keyword '"Jagarlamudi, K. K."' showing total 1 results
Start Over Author "Jagarlamudi, K. K." Topic leukemia
1 results on '"Jagarlamudi, K. K."'

1. Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development.

Author

Jagarlamudi KK, Wang L, and Eriksson S

Subjects
Biomarkers, Pharmacological metabolism, Cell Line, Tumor, Cell Survival drug effects, Drug Development, Female, Humans, Signal Transduction, Antineoplastic Agents pharmacology, Breast Neoplasms drug therapy, Doxorubicin pharmacology, Leukemia drug therapy, Thymidine Kinase metabolism
Abstract

In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 µM Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 µM of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results