1. P15INK4B gene methylation and expression in normal, myelodysplastic, and acute myelogenous leukemia cells and in the marrow cells of cured lymphoma patients.
- Author
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Preisler HD, Li B, Chen H, Fisher L, Nayini J, Raza A, Creech S, and Venugopal P
- Subjects
- Adult, Aged, Antigens, CD34, Bone Marrow Cells metabolism, Bone Marrow Cells pathology, Cyclin-Dependent Kinase Inhibitor p15, Hematologic Diseases genetics, Hematologic Diseases metabolism, Humans, Leukemia, Myeloid, Acute metabolism, Lymphoma metabolism, Middle Aged, Myelodysplastic Syndromes metabolism, Neoplasms, Second Primary genetics, Neoplasms, Second Primary metabolism, RNA, Messenger metabolism, Remission Induction, Cell Cycle Proteins genetics, Cyclin-Dependent Kinase Inhibitor p16 genetics, DNA Methylation, Leukemia, Myeloid, Acute genetics, Lymphoma genetics, Lymphoproliferative Disorders genetics, Lymphoproliferative Disorders metabolism, Myelodysplastic Syndromes genetics, Tumor Suppressor Proteins
- Abstract
P15INK4B methylation and expression was studied in bone marrow cells obtained from normal individuals, from patients who had been cured of lymphoma, and from patients with either MDS or AML. The level of p15 methylation was very low in normal BM cells and in CD34+ and CD34- subpopulations (0-6.5%; med, = 2.5%). P15INK4B transcripts were present in each of these cell populations. In contrast, methylation was the usual situation in MDS and AML marrows. The presence of methylation of the p15INK4B gene did not always indicate an absence of expression nor was expression always present if methylation was absent. P15INK4B methylation was studied in the marrows of nine patients (one studied twice) who had been cured of lymphoma and in whom hemopoiesis was believed to be normal. Increased methylaton was present in all 10 marrows. These data indicate that p15INK4B methylation is likely to be a very early event in the development of the secondary hematologic disorders.
- Published
- 2001
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