1. First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil.
- Author
-
Rêgo FD, da Silva ES, Lopes VV, Teixeira-Neto RG, Belo VS, Fonseca Júnior AA, Pereira DA, Pena HP, Laurenti MD, Araújo GV, da Matta VLR, Chouman IH, Burrin TB, Sandoval CM, Barrouin-Melo SM, de Pinho FA, de Andrade HM, Nunes RV, Gontijo CMF, Soccol VT, Klocek D, Grybchuk D, Macedo DH, do Monte-Neto RL, Yurchenko V, and Soares RP
- Subjects
- Humans, Animals, Dogs, Brazil, RNA-Dependent RNA Polymerase, Leishmania infantum genetics, Leishmaniasis, Visceral veterinary
- Abstract
Background: Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania., Objectives: In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions., Methods: A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene., Findings: We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, respectively., Main Conclusions: To the best of our knowledge, this is the first detection of LRV2 in the New World.
- Published
- 2023
- Full Text
- View/download PDF