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Your search keyword '"Edson H. Teixeira"' showing total 3 results
Start Over Author "Edson H. Teixeira" Topic lectin
3 results on '"Edson H. Teixeira"'

1. Purification, biochemical characterization of a lectin from marine sponge Ircinia strobilina and its effect on the inhibition of bacterial biofilms

Author

ALEXANDRA S. DE ALMEIDA, DAYARA N.M. MENDONÇA, RÔMULO F. CARNEIRO, ULISSES PINHEIRO, ELIELTON FRANCISCO DO NASCIMENTO, ALEXANDRE L. ANDRADE, MAYRON A. DE VASCONCELOS, EDSON H. TEIXEIRA, CELSO S. NAGANO, and ALEXANDRE H. SAMPAIO

Subjects
marine sponge, lectin, antibiofilm effect, amino acid sequencing, Science
Abstract

Abstract A new lectin from marine sponge Ircinia strobilina, denominated IsL, was isolated by combination of affinity chromatography in Guar gum matrix followed by size exclusion chromatography. IsL was able to agglutinate native and enzymatically treated rabbit erythrocytes, being inhibited by galactosides, such as α-methyl-D-galactopyranoside, β-methyl-D-galactopyranoside and α-lactose. IsL hemagglutinating activity was stable at neutral to alkaline pH, however the lectin loses its activity at 40° C. The molecular mass determinated by mass spectrometry was 13.655 ± 5 Da. Approximately 40% of the primary structure of IsL was determined by mass spectrometry, but no similarity was observed with any protein. The secondary structure of IsL consists of 28% α-helix, 26% β-sheet, and 46% random region, as determined by dichroism circular. IsL was a calcium-dependent lectin, but no significant variations were observed by circular dichroism when IsL was incubated in presence of calcium and EDTA. IsL was not toxic against Artemia nauplii and did not have antimicrobial activity against bacterial cells. However, the IsL was able to significantly inhibit the biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis.

Published
2023
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2. New lectin isolated from the tropical sponge Haliclona (Reniera) implexiformis (Hechtel, 1965) shows antibiofilm effect

Author

FRANCISCO R.N. ANDRADE, PEDRO A.S. TABOSA, RENATO C.F. TORRES, RÔMULO F. CARNEIRO, MAYRON A. VASCONCELOS, ALEXANDRE L. ANDRADE, ELIELTON NASCIMENTO, ULISSES PINHEIRO, EDSON H. TEIXEIRA, CELSO S. NAGANO, and ALEXANDRE H. SAMPAIO

Subjects
sponge, Multidisciplinary, purification, lectin, Antibiofilm, circular dichroism, mass spectrometry
Abstract

A lectin from the marine sponge Haliclona (Reniera) implexiformis (HiL) was isolated by affinity chromatography on Sepharose™ matrix. HiL showed specificity for galactose and its derivatives. The glycoproteins porcine stomach mucin (PSM) and bovine stomach mucin (BSM) were potent inhibitors. Hemagglutinating activity of the lectin was maximal between pH 5.0 and 9.0. The lectin remained active until 60°C. The presence of CaCl2 and EDTA did not affect the hemagglutinating activity. In SDS-PAGE, HiL showed a single band of 20 kDa under reduced conditions, whereas in the non-reducing conditions, it showed a band of 20 kDa and one additional band of 36 kDa. The average molecular mass determined by Electrospray Ionization Mass Spectrometry (ESI-MS) was 35.874 ± 2 Da in native and non-reducing conditions, whereas carboxyamidomethylated-lectin showed 18,111 Da. These data indicated that HiL consists in a dimer formed by identical subunits linked by disulfide bonds. Partial amino acid sequence of HiL was determined by mass spectrometry, and revealed that it is a new type of lectin, which showed no similarity with any protein. Secondary structure consisted of 6% α-helice, 31% β-sheet, 18% β-turn and 45% random coil. HiL showed significant reduction in the number of viable cells of Staphylococcus biofilms.

Published
2023

3. Renal alterations promoted by the lectins from Canavalia ensiformis (ConA) and Dioclea guianensis (DguiL) seeds

Author

Alexandre, Havt, Paulo S F, Barbosa, Ticiana M, Sousa, Alice M C, Martins, Arlandia C L, Nobre, Kyria S, Nascimento, Edson H, Teixeira, Vicente P T, Pinto, Alexandre H, Sampaio, Manassés C, Fonteles, Benildo S, Cavada, and Helena S A, Monteiro

Subjects
Male, biology, Potassium, Rat kidney, Lectin, chemistry.chemical_element, chemical and pharmacologic phenomena, General Medicine, biology.organism_classification, Kidney, Biochemistry, Rats, Canavalia, chemistry, Structural Biology, Canavalia ensiformis, Seeds, biology.protein, Animals, Dioclea guianensis, Plant Lectins, Rats, Wistar, Perfusion
Abstract

The lectin from the seeds of Canavalia ensiformis (ConA) and Dioclea guianensis (DguiL) was tested upon its renal effects using the isolated perfusion rat kidney method. Both lectins (10 microg/ml) affected perfusion pressure and renal vascular resistance, but DguiL showed a much greater action than ConA. However, ConA, but not DguiL, affected potassium tubular transport.

Published
2003

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