6 results on '"DAUZAT, MYRIAM"'
Search Results
2. Combined Genetic and Modeling Approaches Reveal That Epidermal Cell Area and Number in Leaves Are Controlled by Leaf and Plant Developmental Processes in Arabidopsis
- Author
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Tisné, Sébastien, Reymond, Matthieu, Vile, Denis, Fabre, Juliette, Dauzat, Myriam, Koornneef, Maarten, and Granier, Christine
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- 2008
- Full Text
- View/download PDF
3. Natural variation of Arabidopsis thaliana responses to Cauliflower mosaic virus infection upon water deficit
- Author
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Bergès, Sandy, Vasseur, François, Bediée, Alexis, Rolland, Gaëlle, Masclef, Diane, Dauzat, Myriam, van Munster, Manuella, Vile, Denis, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, rant #2015005464 from European Union and the Region LanguedocRoussillon 'Chercheur d'Avenir' (FEDER FSE IEJ 2014-2020, Project APSEVIR)., Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
- Subjects
Leaves ,Genotype ,QH301-705.5 ,Arabidopsis Thaliana ,[SDV]Life Sciences [q-bio] ,Arabidopsis ,Plant Pathogens ,Plant Science ,Brassica ,Research and Analysis Methods ,Microbiology ,Plant Viral Pathogens ,Model Organisms ,Caulimovirus ,Plant and Algal Models ,Virology ,Plant Resistance to Abiotic Stress ,Plant-Environment Interactions ,Natural Resources ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Plant Defenses ,Biology (General) ,Flowering Plants ,ComputingMilieux_MISCELLANEOUS ,Plant Diseases ,Dehydration ,Ecology ,Plant Anatomy ,Plant Ecology ,Ecology and Environmental Sciences ,fungi ,Organisms ,Genetic Variation ,Biology and Life Sciences ,Eukaryota ,food and beverages ,Plants ,Plant Pathology ,RC581-607 ,[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy ,[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding ,Experimental Organism Systems ,Plant Physiology ,Animal Studies ,Water Resources ,Immunologic diseases. Allergy ,Viral Transmission and Infection ,Research Article - Abstract
Plant virus pathogenicity is expected to vary with changes in the abiotic environment that affect plant physiology. Conversely, viruses can alter the host plant response to additional stimuli from antagonism to mutualism depending on the virus, the host plant and the environment. Ecological theory, specifically the CSR framework of plant strategies developed by Grime and collaborators, states that plants cannot simultaneously optimize resistance to both water deficit and pathogens. Here, we investigated the vegetative and reproductive performance of 44 natural accessions of A. thaliana originating from the Iberian Peninsula upon simultaneous exposure to soil water deficit and viral infection by the Cauliflower mosaic virus (CaMV). Following the predictions of Grime’s CSR theory, we tested the hypothesis that the ruderal character of a plant genotype is positively related to its tolerance to virus infection regardless of soil water availability. Our results showed that CaMV infection decreased plant vegetative performance and annihilated reproductive success of all accessions. In general, water deficit decreased plant performance, but, despite differences in behavior, ranking of accessions tolerance to CaMV was conserved under water deficit. Ruderality, quantified from leaf traits following a previously published procedure, varied significantly among accessions, and was positively correlated with tolerance to viral infection under both well-watered and water deficit conditions, although the latter to a lesser extent. Also, in accordance with the ruderal character of the accession and previous findings, our results suggest that accession tolerance to CaMV infection is positively correlated with early flowering. Finally, plant survival to CaMV infection increased under water deficit. The complex interactions between plant, virus and abiotic environment are discussed in terms of the variation in plant ecological strategies at the intraspecific level., Author summary Virus pathogenicity may be influenced by changes in the abiotic environment. A common change is decrease in soil water availability, which is detrimental to plant productivity and the occurrence of which is expected to increase due to climate change, has recently been shown to interfere with plant–virus interactions. We investigated the performance of 44 natural accessions of the plant species Arabidopsis thaliana infected by Cauliflower mosaic virus under well-watered and water deficit conditions. We showed that viral infection decreased plant vegetative performance and annihilated reproductive success of all accessions, and that these pathogenic effects were increased by water deficit. Intrinsic characteristics of the accessions were related to their tolerance to the virus so that accessions with low leaf tissue density and rapid growth rate were more tolerant to viral infection regardless of watering condition. Finally, plant survival upon viral infection increased under water deficit. We discuss the role of intrinsic plant characteristics, seen as ecological strategies, in plant tolerance to viral infections under contrasting environmental conditions, and the consequences for the study of viral epidemiology.
- Published
- 2020
4. Are compound leaves more complex than simple ones? A multi-scale analysis.
- Author
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Koch, Garance, Rolland, Gaëlle, Dauzat, Myriam, Bédiée, Alexis, Baldazzi, Valentina, Bertin, Nadia, Guédon, Yann, and Granier, Christine
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LEAVES ,EPIDERMAL growth factor ,ANTISENSE genetics ,PHENOTYPES ,TRANSGENIC animals - Abstract
Background and Aims The question of which cellular mechanisms determine the variation in leaf size has been addressed mainly in plants with simple leaves. It is addressed here in tomato taking into consideration the expected complexity added by the several lateral appendages making up the compound leaf, the leaflets. Methods Leaf and leaflet areas, epidermal cell number and areas, and endoreduplication (co-) variations were analysed in Solanum lycopersicum considering heteroblastic series in a wild type (Wva106) and an antisense mutant, the Pro
35S : Slccs52AAS line, and upon drought treatments. All plants were grown in an automated phenotyping platform, PHENOPSIS, adapted to host plants grown in 7 L pots. Key Results Leaf area, leaflet area and cell number increased with leaf rank until reaching a plateau. In contrast, cell area slightly decreased and endoreduplication did not follow any trend. In the transgenic line, leaf area, leaflet areas and cell number of basal leaves were lower than in the wild type, but higher in upper leaves. Reciprocally, cell area was higher in basal leaves and lower in upper leaves. When scaled up at the whole sympodial unit, all these traits did not differ significantly between the transgenic line and the wild type. In response to drought, leaf area was reduced, with a clear dose effect that was also reported for all size-related traits, including endoreduplication. Conclusions These results provide evidence that all leaflets have the same cellular phenotypes as the leaf they belong to. Consistent with results reported for simple leaves, they show that cell number rather than cell size determines the final leaf areas and that endoreduplication can be uncoupled from leaf and cell sizes. Finally, they re-question a whole-plant control of cell division and expansion in leaves when the Wva106 and the Pro35S : Slccs52AAS lines are compared. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
5. PYM: a new, affordable, image-based method using a Raspberry Pi to phenotype plant leaf area in a wide diversity of environments.
- Author
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Valle, Benoît, Simonneau, Thierry, Boulord, Romain, Sourd, Francis, Frisson, Thibault, Ryckewaert, Maxime, Hamard, Philippe, Brichet, Nicolas, Dauzat, Myriam, and Christophe, Angélique
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RASPBERRY Pi ,PHENOTYPES ,LEAVES ,BIOLOGICAL systems ,IMAGE processing - Abstract
Background: Plant science uses increasing amounts of phenotypic data to unravel the complex interactions between biological systems and their variable environments. Originally, phenotyping approaches were limited by manual, often destructive operations, causing large errors. Plant imaging emerged as a viable alternative allowing non-invasive and automated data acquisition. Several procedures based on image analysis were developed to monitor leaf growth as a major phenotyping target. However, in most proposals, a time-consuming parameterization of the analysis pipeline is required to handle variable conditions between images, particularly in the field due to unstable light and interferences with soil surface or weeds. To cope with these difficulties, we developed a low-cost, 2D imaging method, hereafter called PYM. The method is based on plant leaf ability to absorb blue light while reflecting infrared wavelengths. PYM consists of a Raspberry Pi computer equipped with an infrared camera and a blue filter and is associated with scripts that compute projected leaf area. This new method was tested on diverse species placed in contrasting conditions. Application to field conditions was evaluated on lettuces grown under photovoltaic panels. The objective was to look for possible acclimation of leaf expansion under photovoltaic panels to optimise the use of solar radiation per unit soil area. Results: The new PYM device proved to be efficient and accurate for screening leaf area of various species in wide ranges of environments. In the most challenging conditions that we tested, error on plant leaf area was reduced to 5% using PYM compared to 100% when using a recently published method. A high-throughput phenotyping cart, holding 6 chained PYM devices, was designed to capture up to 2000 pictures of field-grown lettuce plants in less than 2 h. Automated analysis of image stacks of individual plants over their growth cycles revealed unexpected differences in leaf expansion rate between lettuces rows depending on their position below or between the photovoltaic panels. Conclusions: The imaging device described here has several benefits, such as affordability, low cost, reliability and flexibility for online analysis and storage. It should be easily appropriated and customized to meet the needs of various users. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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6. Keep on growing under drought: genetic and developmental bases of the response of rosette area using a recombinant inbred line population S. Tisné et al. Leaf development and drought stress.
- Author
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TISNÉ, SÉBASTIEN, SCHMALENBACH, INGA, REYMOND, MATTHIEU, DAUZAT, MYRIAM, PERVENT, MARJORIE, VILE, DENIS, and GRANIER, CHRISTINE
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ARABIDOPSIS thaliana ,ARABIDOPSIS ,PLANT water requirements ,LEAVES ,EPIDERMIS - Abstract
Variation in leaf development caused by water deficit was analysed in 120 recombinant inbred lines derived from two Arabidopsis thaliana accessions, L er and An-1. Main effect quantitative trait loci (QTLs) and QTLs in epistatic interactions were mapped for the responses of rosette area, leaf number and leaf 6 area to water deficit. An epistatic interaction between two QTLs affected the response of whole rosette area and individual leaf area but only with effects in well-watered condition. A second epistatic interaction between two QTLs controlled the response of rosette area and leaf number with specific effects in the water deficit condition. These effects were validated by generating and phenotyping new appropriate lines. Accordingly, a low reduction of rosette area was observed for lines with a specific allelic combination at the two interacting QTLs. This low reduction was accompanied by an increase in leaf number with a lengthening of the vegetative phase and a low reduction in individual leaf area with low reductions in epidermal cell area and number. Statistical analyses suggested that responses of epidermal cell area and number to water deficit in individual leaves were partly caused by delay in flowering time and reduction in leaf emergence rate, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
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