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The rhizosphere is an important hotspot for microbial activity, organic carbon input, and carbon turnover in soils. The interplay of these rhizosphere components results in small scale gradients of organic molecules in the zone around a root. Mass spectrometric imaging (MSI) can reveal the spatial distribution of individual plant metabolites in the soil, which cannot be achieved using bulk analysis. Using non-fragmenting ionization techniques such as laser desorption ionization (LDI) allows for the detection of intact molecules without the need for labeling with e.g. fluorescent tags.Direct MSI for the chemical imaging of intact molecules of the rhizosphere has been recognized as a still existing analytical gap. Here we present a novel method allowing mass spectrometric molecular rhizosphere imaging directly in a complex soil matrix.Our novel approach consists of sampling the roots and the surrounding soil of Zea mays plants in either field- or lab-scale experiments using small metal cylinders. After excavation, the loam soil pellets were embedded in gelatin and cryosectioned to 100 µm sections. After selecting regions of interest on the soil section, the root and the soil surrounding the root was analysed using ultra-high resolution laser desorption ionization Fourier-transform ion cyclotron resonance mass spectrometry (LDI-FT-ICR-MS).Given the large background of soil-derived organic carbon, the high mass resolution and sensitivity of FT-ICR-MS allow distinguishing root-derived molecules from soil organic matter based on their exact masses. We show that our method is capable to recover rhizosphere gradients of a dihexose (C12H22O11, e.g. sucrose, maltose) directly in the soil with a spatial resolution of 25 µm.Molecular gradients for the dihexose showed a high abundance of this metabolite in the root and a strong depletion of the signal intensity within 150 µm from the root surface. Analysing several sections from the same soil pellet allowed to recover 3D molecular gradients from one root segment. Utilizing the potential to easily change the mass window a variety of potential metabolites can be analysed in the same region around the root. Thus the chemical diversity of potential root exudates can be revealed.Our workflow enables the study of root-derived organic carbon with high spatial resolution directly in a soil context. For the first time, direct molecular imaging of the rhizosphere via LDI-FT-ICR-MS will allow for a non-target or targeted analysis of complex soil samples.Visualizing the root structure via X-ray computed tomography in a soil sample before the embedding would enable a guided sampling approach to analyse molecular distributions at certain parts of the root. Moreover, the molecular LDI-MSI results could be correlated with elemental imaging via laser ablation – inductively coupled plasma – mass spectrometry directly at the same sample position - allowing for an even more detailed insight into chemical processes in the rhizosphere.

To date, several numerical models predicting the properties of TIG-arcs are available. Recently, effort has been put into testing the reliability of these models for varying process parameters by means of comparing plasma temperatures and arc voltages against measured data. However, from an engineering point of view, the goal of these models is to predict the properties of weld beads. Therefore, the heat input into and the pressure applied onto the workpiece have to be predicted. This paper deals with the comparison of measured arc pressure data and the results of an approach which couples a simplified cathode sheath model with an LTE arc model.

Laser-based joining is a potential key manufacturing process for realizing metal plastic hybrids. Therefore, surface preparation is essential to achieve a mechanical form fit. A new approach for surface preparation is based on a tungsten inert gas (TIG) arc process with anodic polarity. This TIG structure was characterized compared to fiber laser manufactured structures in cw and pw mode. The comparison of different preparations was based on pure ultimate tensile strength tests on spot joints. Finally, a transfer to overlap joints was successfully carried out and characterized by tensile shear tests using fiber reinforced plastics.

To date, several numerical models representing the tungsten inert gas (TIG) arc are available. However, little has been done on testing the reliability of these models for parameter values differing from the ones used in the respective papers. This paper deals with the comparison of measured arc welding data provided by E. Siewert from Linde AG and the results of the two most common arc and cathode sheath models. These models make different degrees of simplification of the actual processes in the arc, especially the cathode sheath. Different shapes of the electrodes, different welding currents and shielding gases are taken into account as well as the resolution of the numerical grid on which the calculations are carried out. The findings of this paper are used to show when a simplified approach still leads to valid results and when more sophisticated models have to be used.

In this paper, the results for keyhole welding of stainless steel 1.4301 and mild steel 1.0117 with a cathode focussed gas tungsten arc process are presented. These tests were carried out in preparation for welding the root faces of Y- or double V-groove weld preparations of thick metal plates. Welded plate thicknesses range from 6 to 10 mm for stainless steels and 6 to 12 mm for mild steels. Welding speeds are in the range of 20 to 115 cm/min at currents from 400 to 800 A. Especially for stainless steel, a big range of applicable and stable process parameters could be found. The process also works fine for mild steel even though the achievable parameter range is smaller.

This paper presents extensive investigations about the influence of He-additions to argon TIG-arc properties. Results of diagnostic methods, numerical simulations and welding trials are combined to improve the comprehension about the mode of action of gas mixtures. Additionally, selected results of the investigation with H2- and N2-additions are summarized. The investigations show that all gas additions cause an increase of the heat input into the workpiece. However, the stagnation pressure depends on the gas composition: He-additions result in a decrease of the stagnation pressure which depends on the arc length, whereas H2-and N2-additions increase the pressure. By a systematic choice of the gas mixture the weld depth and also the maximum feed speed can be influenced.

Achievable benefits in hybrid laser-arc welding are closely related to suitable parameter settings. Basic optimizations consequently need a profound understanding of the relevance of involved interaction mechanisms. These are however differently evaluated and discussed in literature. This paper gives an over-view on the most popular hypotheses in the field of laser-arc processing. The importance of direct interactions between laser radiation and arc plasma as well as the role of metal evaporation are particularly considered by own experimental and numerical investigations. The results of this analysis suggest that these phenomena cannot be regarded as main driving mechanisms for synergistic achievements in hybrid laser-arc welding with respect to process stability and performance.Achievable benefits in hybrid laser-arc welding are closely related to suitable parameter settings. Basic optimizations consequently need a profound understanding of the relevance of involved interaction mechanisms. These are however differently evaluated and discussed in literature. This paper gives an over-view on the most popular hypotheses in the field of laser-arc processing. The importance of direct interactions between laser radiation and arc plasma as well as the role of metal evaporation are particularly considered by own experimental and numerical investigations. The results of this analysis suggest that these phenomena cannot be regarded as main driving mechanisms for synergistic achievements in hybrid laser-arc welding with respect to process stability and performance.

The paper presents a transient three-dimensional model of an anti-phase-synchronized pulsed tandem gas–metal arc welding process, which is used to analyse arc interactions and their influence on the gas shield flow. The shielding gases considered are pure argon and a mixture of argon with 18% CO2. Comparison of the temperature fields predicted by the model with high-speed images indicates that the essential features of the interactions between the arcs are captured. The paper demonstrates strong arc deflection and kinking, especially during the low-current phase of the pulse, in agreement with experimental observations. These effects are more distinct for the argon mixture with 18% CO2. The second part of the paper demonstrates the effects of arc deflection and instabilities on the shielding gas flow and the occurrence of air contamination in the process region. The results allow an improved understanding of the causes of periodic instabilities and weld seam imperfections such as porosity, spatter, heat-tint oxidation and fume deposits.