1. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
- Author
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Elia A Junco, Hartawan Laksmono, S. Michael Soltis, Tara Michels-Clark, Christina Y. Hampton, Mengning Liang, Claudiu A. Stan, Iris D. Young, Aaron S. Brewster, Raymond G. Sierra, Franklin D. Fuller, Hasan DeMirci, Michael J. Bogan, Ruchira Chatterjee, Sheraz Gul, Brandon Hayes, Nicholas K. Sauter, Mark S. Hunter, Sébastien Boutet, Jason E. Koglin, Andrew Aquila, Vittal K. Yachandra, Junko Yano, Joseph D. Puglisi, Cornelius Gati, Mohamed Ibrahim, Elisabetta Viani Puglisi, E. Han Dao, Jan Kern, and Athina Zouni
- Subjects
Models, Molecular ,0301 basic medicine ,Technology ,Materials science ,Photosystem II ,macromolecular substances ,ambient temperature crystallography ,Medical and Health Sciences ,Biochemistry ,Ribosome ,Article ,law.invention ,03 medical and health sciences ,Electrokinetic phenomena ,Models ,Thermolysin ,law ,antibiotic ,SFX ,Geobacillus stearothermophilus ,Mother liquor ,Molecular Biology ,electrospinning ,concentric-flow ,Crystallography ,biology ,photosystem II ,Photosystem II Protein Complex ,Molecular ,Cell Biology ,Injector ,Biological Sciences ,Thermus thermophilus ,biology.organism_classification ,030104 developmental biology ,ribosome ,bacteria ,Ribosomes ,Developmental Biology ,Biotechnology - Abstract
In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-temperature X-ray crystal structure of the 30S subunit bound to the antibiotic paromomycin was obtained in its native mother liquor. Compared to previous cryo-cooled structures, this new structure showed that paromomycin binds to the decoding center in a different conformation.
- Published
- 2015