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Objective·To analyze infertility couples, dyadic coping level by using latent profile analysis (LPA), and explore the heterogeneity and related factors of different profiles.Methods·From September to November 2023, 257 newly diagnosed infertility couples in pre-infertility treatment with assisted reproductive technology (ART) were recruited from Reproductive Medicine Center, Renji Hospital, Shanghai Jiao Tong University School of Medicine. All couples were evaluated by using general information questionnaire, Fertility Problem Inventory (FPI), Dyadic Coping Inventory (DCI), and Fertility Quality of Life (FertiQoL) Tool. LPA was used to explore the dyadic coping profiles of the couples before ART treatment, and general information, FPI scores and FertiQoL scores were compared among the profiles. Multinomial Logistic regression analysis was used to explore the related factors of different profiles.Results·A total of 257 couples with infertility were included, with an average age of (30.15±3.07) years for females, (31.82±3.82) years for males, (3.75±2.16) years for marriage, and (2.90±1.92) years for infertility; there were 118 couples caused by male infertility, 109 couples caused by female infertility, and 30 couples caused by both infertility; the average DCI score for males was (128.25±19.15) points, while for females it was (129.91±18.32) points. According to the dyadic coping levels, the infertile couples were divided into four profiles: common positive coping group (153 couples, 59.5%), common negative coping group (85 couples, 33.1%), male positive coping group (12 couples, 4.7%), and male negative coping group (7 couples, 2.7%). There were statistically significant differences in the infertile couples' age, FPI score, FertiQoL score, and remarriage rate among the four profiles (P