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Background: Recently, laser systems have become available which generate ultrashort laser pulses with a duration of 100-200 femtoseconds (fs). By generating micro-plasmas inside the corneal stroma with fs pulses, it is possible to achieve a cutting effect inside the tissue while leaving the anterior layers intact. The energy threshold to generate a micro-plasma with fs pulses is some orders of magnitude lower than it is for picosecond or nanosecond pulses. This results in a strong reduction of the thermal and mechanical damage of the surrounding tissue., Methods: With a titanium:sapphire fs laser system, the cutting effect on corneal tissue from freshly enucleated porcine eye globes was investigated with different pulse energies. The irradiated samples were examined by light and electron microscopy. The laser-induced pressure transients and the laser-induced bubble formation were analysed with a broadband acoustic transducer and by flash photography., Results: With fs laser pulses, the extent of thermal and mechanical damage of the adjacent tissue is in the order of 1 microm or below and therefore comparable with the tissue alterations after ArF excimer laser ablation. Using pulse energies of approximately 1-2 microJ and a spot diameter of 5-10 microm, intrastromal cuts can be performed very precisely in order to prepare corneal flaps and lenticules., Conclusion: Femtosecond photodisruption has the potential to become an attractive tool for intrastromal refractive surgery.