1. High-speed high-resolution laser diode-based photoacoustic microscopy for in vivo microvasculature imaging
- Author
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Xiufeng Li, Victor T C Tsang, Lei Kang, Yan Zhang, and Terence T W Wong
- Subjects
Photoacoustic microscopy ,Laser diode ,In vivo imaging ,Microvasculature imaging ,Drawing. Design. Illustration ,NC1-1940 ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Computer software ,QA76.75-76.765 - Abstract
Abstract Laser diodes (LDs) have been considered as cost-effective and compact excitation sources to overcome the requirement of costly and bulky pulsed laser sources that are commonly used in photoacoustic microscopy (PAM). However, the spatial resolution and/or imaging speed of previously reported LD-based PAM systems have not been optimized simultaneously. In this paper, we developed a high-speed and high-resolution LD-based PAM system using a continuous wave LD, operating at a pulsed mode, with a repetition rate of 30 kHz, as an excitation source. A hybrid scanning mechanism that synchronizes a one-dimensional galvanometer mirror and a two-dimensional motorized stage is applied to achieve a fast imaging capability without signal averaging due to the high signal-to-noise ratio. By optimizing the optical system, a high lateral resolution of 4.8 μm has been achieved. In vivo microvasculature imaging of a mouse ear has been demonstrated to show the high performance of our LD-based PAM system.
- Published
- 2021
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