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Fluorescent nanoparticles, such as quantum dots, hold great potential for biomedical applications, mainly sensing and bioimaging. However, the inefficient cell uptake of some nanoparticles hampers their application in clinical practice. Here, the effect of the modification of the quantum dot surface with fluorinated ligands to increase their surface activity and, thus, enhance their cellular uptake was explored. Fluorination of nanoparticles (NPs) is presented as a strategy to promote the interactions between NPs and the cell membrane, resulting in efficient cellular uptake of NPs. The increased surface activity of fluorinated NPs, which is demonstrated by using Langmuir monolayers as cell membrane models, seems to be the key feature for their enhanced cellular uptake. [ABSTRACT FROM AUTHOR]

Abstract: Physico-chemical processes at air/liquid interfaces are of paramount importance in nature. The Langmuir technique offers the possibility of forming a well-defined monolayer of amphiphilic molecules under study at the air/liquid interface, with a unique control of the area per molecule and other experimental conditions. Despite being a traditional technique in Colloid and Interface science, there is an ever growing interest in Langmuir studies. Herein, recent developing fields of research currently taking advantage of the Langmuir technique are reviewed, comprising the interfacial structure of: water, biomolecules and inorganic/organic hybrids. The good state of the Langmuir technique at present and the foreseeable increase of its usage are discussed. [Copyright &y& Elsevier]

Abstract: This work focuses on the interaction of mycolic acids (MAs) and two antimycobacterial compounds (Rifabutin and N′-acetyl-Rifabutin) at the pulmonary membrane level to convey a biophysical perspective of their role in disease. For this purpose, accurate biophysical techniques (Langmuir isotherms, Brewster angle microscopy, and polarization-modulation infrared reflection spectroscopy) and lipid model systems were used to mimic biomembranes: MAs mimic bacterial lipids of the Mycobacterium tuberculosis (MTb) membrane, whereas Curosurf® was used as the human pulmonary surfactant (PS) membrane model. The results obtained show that high quantities of MAs are responsible for significant changes on PS biophysical properties. At the dynamic inspiratory surface tension, high amounts of MAs decrease the order of the lipid monolayer, which appears to be a concentration dependent effect. These results suggest that the amount of MAs might play a critical role in the initial access of the bacteria to their targets. Both molecules also interact with the PS monolayer at the dynamic inspiratory surface. However, in the presence of higher amounts of MAs, both compounds improve the phospholipid packing and, therefore, the order of the lipid surfactant monolayer. In summary, this work discloses the putative protective effects of antimycobacterial compounds against the MAs induced biophysical impairment of PS lipid monolayers. These protective effects are most of the times overlooked, but can constitute an additional therapeutic value in the treatment of pulmonary tuberculosis (Tb) and may provide significant insights for the design of new and more efficient anti-Tb drugs based on their behavior as membrane ordering agents. [Copyright &y& Elsevier]

Abstract: The Brewster Angle Microscopy (BAM) is a powerful microscopy technique allowing the in situ visualization of the morphology of Langmuir monolayers at the air/water interface. The use of the BAM for attaining structural insights in the molecular arrangement of the Langmuir monolayers is widespread. In this review, we examine the reflection of a Langmuir monolayer under a rather different perspective than classical: the influence of the polar headgroup of the amphiphiles in the BAM images is taken into account. The relevance of the polar headgroup as the main cause of the BAM features has been the focus of a reduced number of BAM studies. An emerging experimental and theoretical framework from recent bibliography is discussed. Different theoretical scenarios are considered, concerning the size and absorption of radiation of the polar headgroup. Two qualitative examples showing physical phenomena regarding the reflectivity changes in a BAM experiments are discussed. The anisotropy in the BAM images as inner textures is of special interest. Quantitative structural information of the molecular arrangement of the monolayer is obtained by simulating the textures of the domains observed. The quantitative assessment of the detailed molecular arrangement of the polar headgroup by BAM is highly valuable, as this information can hardly be obtained from other experimental techniques. The procedure for extracting quantitative structural data from the experimental BAM pictures is revised in detail from the recent bibliography for further application of this model to different Langmuir monolayers. [Copyright &y& Elsevier]

Biomineralization is of paramount importance for life on Earth. The delicate balance of physicochemical interactions at the interface between organic and inorganic matter during all stages of biomineralization resembles an extremely high complexity. The coordination of this sophisticated biological machinery and physicochemical scenarios is certainly a wonderful show of nature. Understanding of the biomineralization processes is still far from complete. The recent advances in biomineralization research from the Colloid and Interface Science perspective are reviewed herein. The synergy between this two fields of research is demonstrated. The unique opportunities offered by purposefully designed fluid interfaces, mainly Langmuir monolayers are presented. Biomedical applications of biomineral-based nanostructures are discussed, showing their improved biocompatibility and on-demand delivery features. A brief guide to the array of state-of-the-art experimental techniques for unraveling the mechanisms of biomineralization using fluid interfaces is included. In summary, the fruitful and exciting crossroad between Colloid and Interface Science with Biomineralization is exhibited. Unlabelled Image • The relevance of fluid interfaces in biomineralization processes is reviewed. • The niche of the Langmuir technique for studying biomineralization is discussed. • A basic guide on the experimental techniques for studying biogenic minerals at interfaces is provided. • Biomedical applications of nanominerals are examined. [ABSTRACT FROM AUTHOR]

Graphical abstract Abstract Monolayers of a cytosine-based nucleolipid (1,2-dipalmitoyl-sn-glycero-3-(cytidine diphosphate) (ammonium salt), CDP-DG) at basic subphase have been prepared at the air-water interface both in absence and presence of guanine. The formation of the complementary base pairing is demonstrated by combining surface experimental techniques, i.e., surface pressure (π)–area (A), Brewster angle microscopy (BAM), infrared spectroscopy (PM-IRRAS) and computer simulations. A folding of the cytosine-based nucleolipid molecules forming monolayer at the air-water interface occurs during the guanine recognition as absorbate host and is kept during several compression-expansion processes under set experimental conditions. The specificity between nitrogenous bases has been also registered. Finally, mixed monolayers of CDP-DG and a phospholipid (1,2-dimyristoyl-sn-glycero-3-phosphate (sodium salt), DMPA) has been studied and a molecular segregation of the DMPA molecules has been inferred by the additivity rule. [ABSTRACT FROM AUTHOR]

Highlights: [•] Langmuir monolayers with phosphatidylcholines were applied as model of biomembranes. [•] Brewster angle micrographs and π–A isotherms were examined after peroxidation. [•] Trolox, α-tocopherol and peroxidation products induced changes on lipid domains. [•] π–A isotherms showed different profile upon peroxidation conditions. [ABSTRACT FROM AUTHOR]