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Objective: The aim of the present study is to investigate the effects of topiramate on the fat mass/obesity-associated protein (FTO) and on the neuropeptide Y (NPY) level in the hypothalamus depending on the recently increased prevalence of obesity. Method: In this study, twenty-four female rats were divided into four equal groups: Non-obese control, obese control, non-obese topiramate, and obese topiramate. Obese groups were fed with a 40% high-fat diet. At the end of the 9th week, the drug treatment started and the subjects were treated with topiramate once a day for 6 weeks. All animals underwent cardiac perfusion under high-dose anesthesia on the 15th week. Tissues were analyzed using biochemical, histological, and stereological methods. Results: In terms of neuron number in the arcuate nucleus area, a significant difference was observed among all groups (P < 0.01). The neuron number of the non-obese topiramate group was found to be significantly higher than that of the non-obese control group (P < 0.01). In the examination of the ventromedial nucleus of the entire group, it was observed that the neuron number of the non-obese control group was significantly lower than those of the other groups (P < 0.01). A significant increase in the NPY levels of the obese groups compared to the groups treated with topiramate was observed. Furthermore, the amount of the FTO protein increased in obese rats, while FTO and NPY levels decreased in the groups treated with topiramate. Discussion: In conclusion, the mechanism of the effect of topiramate to create a state of obesity is thought to involve the decrease in the levels of NPY and FTO. [ABSTRACT FROM AUTHOR]

Gamma radiation, a form of ionizing radiation, is used in many different areas, especially in the health field and in the treatment of cancer. However, gamma radiation used for therapeutic purposes also has numerous harmful effects on human health. This study was planned to investigate the impacts of exposure to gamma radiation on the hippocampal area and the preventive effects of myricitrin and chebulinic acid against that damage. Thirty-six male Wistar albino rats were randomly divided into six groups. The control group was exposed to no treatment. The chebulinic acid and myricitrin groups were injected with the relevant drug at a dosage of 0.033 mg/kg) (vehicle; normal saline) per day. The gamma groups were placed in a plexiglass test setup with their heads positioned close to the source. The subjects were exposed to radiation with a mixed source containing radioactive Cs-137 and Co-60 isotopes obtained from Ondokuz Mayıs University Physics Department Nuclear Physics Laboratory for 1 h. Gamma radiation was applied 16 mGy for one hour per day for 10 days. The gamma radiation+chebulinic acid and the gamma radiation myricitrin groups also received 0.033 mg/kg per day of these drugs via injection. Immediately after the experimental procedure, all animals were subjected to behavioural tests, and perfused brain tissues were analyzed using stereological methods. Stereological analysis showed that gamma radiation caused a decrease in the numbers of neurons in the hippocampal area (p < 0.01; One-way ANOVA) and that chebulinic acid and myricitrin reduced this decrease (p < 0.01; One-way ANOVA). Decreases in learning and memory capacity were detected in behavioural tests in rats from the Gamma group. The study findings showed that that the adverse health effects of Gamma radiation can be ameliorated using myricitrin and chebulinic acid. Myricitrin was more effective in terms of cell proliferation and defence against oxidative stress than chebulinic acid, and exhibited a more neuroprotective effect. However, more detailed analyses should be performed before using either antioxidant for therapeutic purposes. • Neuron damage in the hippocampal region was observed in subjects exposed to gamma radiation. • Chebulunic acid and myricitrin reduced neuron damage due to gamma radiation. • Cell damage due to gamma radiation also caused a decrease in learning and memory functions, and antioxidant treatment reduced the loss of function. [ABSTRACT FROM AUTHOR]