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A label‐free and fast approach for positive electrochemiluminescence (ECL) imaging of single cells by bipolar nanoelectrode array is proposed. The reduction of oxygen at a platinized gold nanoelectrode array in a closed bipolar electrochemical system is coupled with an oxidative ECL process at the anodic side. For elevating the ECL imaging contrast of single cells, a driving voltage of −2.0 V is applied to in situ generate oxygen confined beneath cells that is subsequently used for ECL imaging at 1.1 V. High oxygen concentration in the confined space resulting from steric hindrance generates prominent oxygen reduction current at the cathodic side and higher ECL intensity at the anodic side, allowing positive ECL imaging of the cells adhesion region with excellent contrast. Cell morphology and adhesion strength can be successfully imaged with high image acquisition rate. This approach opens a new avenue for label‐free imaging of single cells. [ABSTRACT FROM AUTHOR]

Keywords: bipolar nanoelectrodes; electrochemiluminescence; label-free imaging; oxygen concentration gradient; single cells EN bipolar nanoelectrodes electrochemiluminescence label-free imaging oxygen concentration gradient single cells 1 1 1 01/18/22 20220113 NES 220113 B Label-free electrochemiluminescence b imaging of single cells has been achieved through a bipolar nanoelectrode array. Front Cover: Label-Free Electrochemiluminescence Imaging of Single-Cell Adhesions by Using Bipolar Nanoelectrode Array (Chem. Eur. J. 3/2022) Bipolar nanoelectrodes, electrochemiluminescence, label-free imaging, oxygen concentration gradient, single cells. [Extracted from the article]